죽염이 초기인공우식법랑질의 재광화에 미치는 영향

Objectives: Silver diamine fluoride (SDF) offers a minimally invasive and inexpensive alternative to traditional restorative caries treatment. Aim of this study was to evaluate the mineral density value and remineralization effect of 38% SDF solution and 5% sodium fluoride (NaF) varnish on artificial primary tooth dentin caries lesions. Materials and Methods: 54 extracted sound primary molars were demineralized and allocated into two groups: SDF and NaF groups. After pH-cycling, mineral density values (MDV) were assessed with micro computed tomography (micro-CT) and surface morphology were studied via scanning electron microscopy (SEM). Results: After remineralization agents were apllied and specimens pH-cycled, the MDVs of the Region of Interest (ROI) in lesions increased in both NaF and SDF group. The mean MDV of SDF applied specimens was found to be higher than NaF applied specimens at statistically significant level. In the SEM images, it was observed that the dentinal tubules orifices were occluded almost completely in SDF group while the orifices in NaF group were partially occluded. Conclusions: The use of 38% SDF demonstrated a great remineralization effect on primary molar dentin than 5% NaF varnish. Further clinical studies are needed to investigate the effect of 38% SDF on primary molars which remains in the mouth longer than incisors and have a great function on chewing.

The acute toxicity of hydrofluoric acid (HFA) was investigated in a 24-h lethal dose study of intravenous infusion in rats. The lethal dose lowest (LDLo) and LD50 were 13.1 and 17.4 mg/kg, respectively. Harmful systemic effects were also studied 1 h after acute sublethal exposure to HFA. The maximum dose was set at 9.6 mg/kg (LD5). Rats were injected with HFA (1.6, 3.2, 6.4 or 9.6 mg/kg), saline, sodium fluoride (NaF) or HCl solution. NaF and HCl solution concentrations corresponded to the F- and H+ concentrations of 9.6 mg/kg HFA. Blood urea nitrogen (BUN) and Cr were significantly increased in response to HFA concentrations greater than 3.2mg/kg. Acute glomerular dysfunction also occurred at HFA concentrations greater than 3.2 mg/kg. HCO3- and base excess (BE) were significantly decreased in the 6.4 and 9.6 mg/kg groups. Ca2+ was significantly decreased, and K+ was increased in the 9.6 mg/kg group. BUN was significantly increased in the NaF and HFA groups and was increased in the HFA group compared with that in the NaF group. Cr was significantly increased in the HFA group only. HCO3- and BE were significantly decreased in the NaF and HFA groups and were increased in the HFA group compared with values in the NaF group. Ca2+ was significantly decreased in the NaF and HFA groups, and K+ was significantly increased in the NaF and HFA groups. F- exposure directly affected serum electrolytes. Mortality was thought to be due to cardiac arrhythmia resulting from hypocalcemia and hyperkalemia. Metabolic acidosis and renal failure were more severe in response to HFA exposure than in response to NaF exposure because of more free F-, which has strong cytotoxicity, in the HFA group than in the NaF group. Lethal effects of HFA are promoted by exposure routes such as inhalation that cause rapid absorption into the body. Even low exposure to HFA can cause acute renal dysfunction, electrolyte abnormalities and metabolic acidosis. These complications result in a poor prognosis.

Objective To observe the preventive effect of type Ⅱ collagen on experimental rat articular cartilage damage induced by T-2 toxin,to explore molecular biomarkers of articular cartilage damage and repair,and to provide a theoretical basis for control of articular cartilage damage.Methods Eighty Wistar rats were randomly divided into 4 groups according to their body weights:negative control,positive control,high-dose intervention,and low-dose intervention groups,20 rats in each group.Animals in negative control group were fed with standard rat chow,and animals in other three groups were fed with T-2-toxin-contaminated chow( 100 ng/kgfeed).Animals in negative and positive control groups drank distilled water,animals in high-dose intervention and low-dose intervention groups drank water containing type Ⅱ collagen(0.5,5.0 g/L,respectively).These rats were sacrificed after 3 and 5 months,respectively,and bilateral knee joints were collected.Histopathologic changes in hyaline cartilage were examined by light microscope,serum levels of type Ⅱ collagen carboxyl terminal peptide (CTX-Ⅱ ),cartilage oligomeric matrix protein (COMP) and urinary deoxypyridinoline (DPD) were determined by enzyme-linked immunosorbent assay(ELISA).Results HE staining showed,that the positive control articular chondrocytes were disarranged,deformated,degenerated,with necrosis and extensive areas of chondrocyte loss;but the two intervention groups only showed fibril formation and swelling and surface cartilage cells became round,flat cartilage cells decreased in number,and cartilage cells clustered and so on early pathological changes of osteoarthritis.At the ends of 3 month and 5 month experiment,the levels of serum CTX- Ⅱ in different groups were,negative control[(18.77 ± 4.61),(25.07 ± 9.17)μg/L],high-dose intervention[ (21.11 ± 5.02),(33.20 ± 9.74)μg/L ],low-dose intervention [ ( 19.87 ± 4.53 ),( 29.73 ± 9.32 ) μg/L ] and positive control [ ( 24.43 ± 5.23 ),( 39.17 ±10.49 ) μg/L ] ; the levels of serum COMP were,negative control group [ (5.43 ± 2.75 ),( 6.38 ± 2.23 ) μg/L ],highdose intervention group[ (17.27 ± 4.77),(20.32 ± 4.74)μg/L],low-dose intervention group[(20.13 ± 5.07),(19.44 ± 4.92)μg/L] and positive control group[ (21.37 ± 4.72),(24.52 ± 4.26)μg/L].At the end of 3 month,compared with negative control group,the level of serum CTX- Ⅱ in other three groups increased,but only positive control group increased significantly(P ＜ 0.05) ; at the end of 5 month,compared with negative control group,the level of serum CTX-Ⅱ in other three groups increased significantly,and the difference was statistically significant (all P ＜ 0.05),and the level of CTX-Ⅱ in the two intervention groups was significantly lower compared with that of positive control group(all P ＜ 0.05).Compared with negative control group,the level of serum COMP in other groups increased significantly at the end of 3 month (all P ＜ 0.05) and only the level of serum COMP in high-dose intervention group was significantly lower compared with that of positive control group(P ＜ 0.05).At the end of 5 month,compared with negative control group,the level of serum COMP in other three groups increased significantly,the difference were statistically significant (all P ＜ 0.05) ; the levels of serum COMP in the two intervention groups were significantly lower than that of positive control group(all P ＜ 0.05).At the ends of 3 month and 5 month,the content of urinary DPD in negative control group were[ (3.47 ± 2.20),(4.14 ± 1.06)μg/L],positive control group[ (4.09 ± 2.48),(4.33 ± 3.43)μg/L],high-dose intervention group[ (3.86 ± 2.31 ),(5.72 ± 3.89)μg/L] and low-dose intervention group[ (3.58 ± 2.77),(4.23 ± 2.90)μg/L].The difference between the 4 groups were not statistically significant (F =2.608,2.436,all P ＞ 0.05).Conclusions Type Ⅱ collagen could effectively reduce the level of serum CTX-Ⅱ and COMP in experimental rats and delay the process of articular cartilage damage induced by T-2 toxin. Key words: Cartilage, articular; Collagen type Ⅱ; T-2 toxin

Background: Dental caries is a prevalent dental problem affecting primary and permanent teeth. Early demineralization of enamel lesions can be reversed through remineralization. Many studies have focused on caries prevention and disease progression arrest using silver diamine fluoride (SDF). No in vitro studies have compared the remineralization effects of different 38% SDF solutions on artificially demineralized enamel lesions. This study aimed to compare the remineralization potential of three commercial 38% silver diamine fluoride formulations on artificial enamel lesions in primary teeth using a pH cycling model. The hypothesis was as follows: different commercial SDF formulations would exhibit varying remineralization effects, as measured by surface microhardness, due to potential differences in their compositions. Materials and Methods: In this study, 75 primary molars were randomized into five groups (N = 15): I: baseline, II: SDF Riva Star Aqua® 38%, III: Riva Star® 38%, IV: SDF Advantage Arrest® 38%, and V: control. Artificial caries were created by submerging teeth in 10 mL of demineralization solution (pH 4.5) for three days in a light-resistant container, ensuring distinct visual changes in the enamel as per the International Caries Detection and Assessment System (ICDAS level 2). After pH cycling, all samples underwent a standardized Vickers microhardness test (VMHT) with a 50 g load for 15 s. Data were analyzed using one-way ANOVA and Tukey’s post hoc test, with a significance level set at p ≤ 0.05. Results: The one-way ANOVA test indicated a significant difference in microhardness among the groups (SDF Riva Star Aqua, SDF Riva Star, and SDF Advantage Arrest), with an F-value of 167.73 and p < 0.001. The post hoc Scheffé test showed that SDF Riva Star Aqua and SDF Riva Star were not significantly different (p = 0.388). However, SDF Advantage Arrest had a significantly higher mean microhardness compared to both groups (p < 0.001). Overall, these results show that SDF Advantage Arrest leads to greater microhardness than SDF Riva Star Aqua or SDF Riva Star. Conclusions: SDF Advantage Arrest showed superior performance among the SDF-treated groups, significantly increasing microhardness compared to SDF Riva Star Aqua and SDF Riva Star. This suggests that SDF Advantage Arrest offers enhanced remineralization and structural strengthening, making it the most effective option for managing demineralized primary teeth. Future research should investigate the long-term performance and mechanisms of these treatments to optimize clinical protocols for preserving primary tooth integrity.

Objective: The motivation of this study was to investigate the effect of grape seed extract on dentin remineralization. Methodology: Sound dentin disks were prepared from the buccal surfaces of human impacted third molars. The specimens were stored in demineralizing solution for 72 hours at room temperature to create artificial carious lesions. They were divided into four groups according to the treatment used; 15% GSE, 1000 ppm sodium fluoride, artificial saliva meanwhile teeth in the fourth group were kept without treatment as a negative control group. An in vitro pH cycling model for eight successive days was done. Subsequently micro-hardness of the specimens and their micro-morphological appearance were evaluated using micro-indenter and Environmental scanning electron microscope (ESEM) respectively. Data were statically analyzed. Results: The results indicated that there was a significant increase in dentin micro-hardness values of GSE and NaF groups in comparison to the demineralized and artificial saliva groups. The ESEM micrographs confirmed the micro-hardness results. Conclusions: GSE is a promising natural remineralizing agent for treatment of the demineralized dentin.

Objective: The mechanism of cerebral ischemia-reperfusion injury is closely related to inflammation, and miRNA-135b-5p may play a key role in the inflammation of cerebral ischemia injury. In this study we investigate the effect of miRNA-135b-5p through GSK3 β-TLR3 signal axis on inflammatory response in mice with cerebral ischemia-reperfusion injury. Methods: 48 mice were randomly divided into sham operation group (n = 12), model group (n = 12), negative control group (n = 12) and overexpression group (n = 12). After neurobehavioral score, the left brain of mice was killed and the water content of brain tissue was measured, the expression levels of TNF- α and IL-6 in brain tissue and serum were detected by ELISA method, the expression levels of TLR3, GSK3β and ERK protein in brain tissue were detected by Western Blot, and the mRNA expression levels of TLR3, GSK3 β and ERK in each group were detected by RT-PCR. Results: The neurobehavioral scores in the model group, negative control group and overexpression group were significantly higher than those in the sham operation group (P < 0.05), and the neurobehavioral scores in the overexpression group were significantly lower than those in the negative control group and model group (P < 0.05). The expression level of miRNA-135b-5p in brain tissue of mice in model group and negative control group was significantly lower than that in sham operation group, while the expression level of miRNA-135b-5p in overexpression group was significantly higher than that in other three groups. The expression levels of TNF- α and IL-6 in brain tissue and serum of mice in model group and negative control group were significantly higher than those in sham operation group, while the expression levels of TNF- α and IL-6 in overexpression group were significantly lower than those in model group and negative control group. The mRNA expression levels of TLR3, GSK3 β and ERK in the model group and negative control group were significantly higher than those in the sham operation group, while the mRNA expression levels of TLR3, GSK3 β and ERK in the overexpression group were significantly lower than those in the model group and negative control group. Conclusion: Overexpression of miRNA-135b-5p could inhibit the expression of GSK3β-TLR3 signal axis related genes and reduce the inflammatory response during cerebral ischemia-reperfusion injury, which may be a new strategy for the treatment of cerebral ischemia-reperfusion injury.

Objective: To explore the effect of down-regulation of retinol binding protein 2 (RBP2) expression on the biological characteristics of ovarian cancer cells and its mechanism. Methods: Knockdown of RBP2 and cisplatin (DDP)-resistant ovarian cancer cell line SKOV3/DDP-RBP2i was established, the negative control group and blank control group were also set. Cell counting kit 8 (CCK-8) was used to detect the cell proliferation ability, flow cytometry was used to detect cell apoptosis, scratch test and Transwell invasion test were used to detect cell migration and invasion ability, real-time fluorescent quantitative polymerase chain reaction (RT-qPCR) and western blot were used to detect the expressions of molecular markers related to epithelial-mesenchymal transition (EMT). The effect of RBP2 on the growth of ovarian cancer was verified through experiment of transplanted tumors in nude mice, and the relationships between RBP2 expression and tumor metastasis and patient prognosis were analyzed using the clinical data of ovarian cancer in TCGA database. Results: After down-regulating the expression of RBP2, the proliferation ability of SKOV3/DDP cell was significantly reduced. On the fifth day, the proliferation activities of SKOV3/DDP-RBP2i group, negative control group and blank control group were (56.67±4.16)%, (84.67±3.51) and (87.00±4.00)% respectively, with statistically significant difference (P<0.001). The apoptosis rate of SKOV3/DDP-RBP2i group was (14.19±1.50)%, higher than (8.77±0.75)% of the negative control group and (7.48±0.52)% of the blank control group (P<0.001). The number of invasive cells of SKOV3/DDP-RBP2i group was (55.20±2.39), lower than (82.60±5.18) and (80.80±7.26) of the negative control group and the blank control group, respectively (P<0.001). The scratch healing rate of SKOV3/DDP-RBP2i group was (28.47±2.72)%, lower than (50.58±4.06)% and (48.92±4.63)% of the negative control group and the blank control group, respectively (P<0.001). The mRNA and protein expressions of E-cadherin in the SKOV3/DDP-RBP2i group were higher than those in the negative control group (P=0.015, P<0.001) and the blank control group (P=0.006, P<0.001). The mRNA and protein expression of N-cadherin in SKOV3/DDP-RBP2i group were lower than those in the negative control group (P=0.012, P<0.001) and the blank control group (P=0.005, P<0.001). The mRNA and protein expressions of vimentin in SKOV3/DDP-RBP2i group were also lower than those in the negative control group (P=0.016, P=0.001) and the blank control group (P=0.011, P=0.001). Five weeks after the cells inoculated into the nude mice, the tumor volume of SKOV3/DDP-RBP2i group, negative control group and blank control group were statistically significant different. The tumor volume of SKOV3/DDP-RBP2i group was smaller than those of negative control group and blank control group (P=0.001). Bioinformatics analysis showed that the expression of RBP2 in patients with metastatic ovarian cancer was higher than that without metastasis (P=0.043), and the median overall survival of ovarian cancer patients with high RBP2 expression was 41 months, shorter than 69 months of low RBP2 expression patients (P<0.001). Conclusion: Downregulation of the expression of RBP2 in SKOV3/DDP cells can inhibit cell migration and invasion, and the mechanism may be related to the inhibition of EMT.

The aim of this study is to describe the remineralization effect of the nano-HA on artificial caries. The artificial dental caries are made by using sour solution, and constructing an artificial mouth' plaque culture system in vitro respectively. The scanning electron microscope (SEM) and the optical microscope are used to observe the surface of enamel specimens. The effect of remineralization is assessed with the polarized light microscopy (PLM) and the confocal laser scanning microscope (CLSM) by measuring three lesion parameters (area, total and average fluorescence). The SEM analysis shows that the cavities and defects of enamel surface are decreased and many mineral salts are sedimentated, which indicate that the nano-HA could promote remineralization for the demineralized enamel. The inhibitory effect of the spherical nano-HA on dental caries is detected in the artificial mouth test. The CLSM shows that demineralization of enamel is reduced by adding the spherical nano-HA in artificial mouth. The results show that the spherical nano HA have a remineralization effect on the artificial dental caries dramatically, and can prevent and decrease caries.

The main purpose of this study was to explore the effects of Spirulina (Spirulina platensis) as a feed supplement by replacing Vitamin-Mineral Premix on the performance of broiler. The study explored that final body weight was 1039, 1070, 1044, 1065, 1117 and 893 g/bird in group 1, 2, 3, 4, 5 and 6 where significantly (p&lt;0.5) higher weight observed in Spirulina group (5) but lower in negative control group (6). Feed intake observed more or less similar in all groups (1483±24g; p&gt;0.5). Feed conversion ratio (kg FI/kg LWG) observed significantly different like 1.72, 1.65, 1.69, 1.62, 1.61 and 1.99 in group 1, 2, 3, 4, 5 and 6 respectively (p&lt;0.5) where higher in negative group (6) and control group (1), but is lowest and similar in 75% Spirulina group (4) and highest (100%) Spirulina group (5). %Ash of different dietary groups were 46.35, 46.54, 48.93, 49.93, 55.07 and 46.92 of group 1, 2, 3, 4, 5 and 6 respectively. The %ash of bone was gradually increased among dietary groups and the best result was obtained in 100% Spirulina group 5 (55.07). It also revealed that diets containing no vitamin-mineral premix with 100% Spirulina improve the performance of broiler. The findings suggest that poultry farmer can use spirulina as a feed item for broiler for more yield.

Objective To explore the role of microRNA- 194(miR- 194)in proliferation, migration,invasion and the epithelial- mesenchymal transition(EMT)of human basal- like breast cancer cell line HCC1937. Methods Three groups were set up:experimental group[HCC1937 with lentivirus- miR - 194- enhanced green fluorescent protein(EGFP), MOI= 50], negative control group(HCC1937 with lentivirus- CON157- EGFP), and blank control group(HCC1937 without lentivirus). The miR- 194 expression was detected by real- time quantitative reverse transcriptase- polymerase chain reaction(RT-qPCR). Cell growth ability of HCC1937 was determined by cell counting kit- 8(CCK- 8)assay. Cell migration and invasion ability of HCC1937 were mearsured by a Transwell assay, and the protein of epithelial - mesenchymal transition phenotype was measured by Western blotting. Results The expression of miR-194 in experimental group(432.93±0.01) was higher than in negative control group(0.90±0.02) and blank control group(1)(P< 0.01) The cells in experimental group grew more slowly[(44.81± 0.11)%]than negative control group[(104.29±0.13)%]and blank control group[(100±0)%, P< 0.01].The number of migration and invasion was both less in experimental group than in negative control group and blank control group(P< 0.05).Western blotting revealed that the expression of E- cadherin in experimental group(0.175±0.053) was significantly decreased as compared with negative control group (0.516±0.032)and blank control group(0.433±0.047, P< 0.05), and the expression of N- cadherin in experimental group(0.342±0.012)was significantly increased as compared with negative control group (0.137±0.007)and blank control group(0.142±0.021)(P<0.05) Conclusion miR- 194 can inhibit proliferation, migration and invasion of basal- like breast cancer cells(HCC1937), and also affect EMT process in HCC1937 cells. Key words: Basal- like breast cancer; MicroRNA- 194; Proliferation

Objective To investigate the expression of lysine-specific demethylase 1 (LSD1) in hepatocellular carcinoma tissues, and to explore the effects of down-regulation of LSD1 gene expression on proliferation and invasion of human hepatoma HepG2 cells. Methods A total of 91 cases of hepatocellular carcinoma undergoing elective surgery in our hospital and Henan Cancer Hospital were selected. Immunohistochemistry was used to detect the expression of LSD1 protein in hepatocellular carcinoma tissues and adjacent tissues. Human hepatoma HepG2 cells were cultured and divided into LSD1 interference sequence group, negative control group and blank group. Real-time fluorescent quantitative polymerase chain reaction (FQ-PCR) was used to detect the expression of LSD1 gene in cells. Methyl thiazol tetrazolium (MTT) assay was used to detect cell proliferation. Transwell chamber was used to detect cell invasion. Western blotting was used to detect the expression of LSD1, Snail, E-cadherin and N-cadherin proteins in cells. Results The positive expression rate of LSD1 protein in hepatocellular carcinoma tissues was 81.32%, which was significantly higher than that in adjacent tissues (35.16%, P<0.01). The expression of LSD1 protein in hepatocellular carcinoma tissues was related to the degree of differentiation, TNM staging and vascular invasion (P<0.05). The relative expression level of LSD1 mRNA in LSD1 interference sequence group was 1.19±0.10, which was significantly lower than that in the negative control group and blank group (2.29±0.14 and 2.34±0.11, P<0.05). The absorbance (A) values at 24, 48, 72 and 96 h in the LSD1 interference sequence group were 0.23±0.05, 0.32±0.08, 0.38±0.08 and 0.52±0.03, respectively, which were significantly lower than those in the negative control group (0.38±0.08, 0.49±0.05, 0.67±0.04 and 0.77±0.10) and blank group (0.33±0.03, 0.52±0.09, 0.70±0.08 and 0.78±0.07, P<0.05). The number of invasive cells in the LSD1 interference sequence group was (86.3±3.9), which was significantly less than that in the negative control group (110.6±9.4) and the blank group (117.7±7.0, P<0.05). The relative expression levels of LSD1, Snail, and N-cadherin proteins in the LSD1 interference sequence group were (0.25±0.03, 0.30±0.02 and 0.32±0.03), which were significantly lower than those in the negative control group (0.77±0.06, 0.76±0.10 and 0.67±0.04) and blank group (0.84±0.14, 0.74±0.07 and 0.69±0.06, P<0.05), and the relative expression level of E-cadherin protein (0.71±0.05) was significantly higher than in the negative control group (0.37±0.02) and blank group (0.34±0.04, P<0.05). Conclusion LSD1 protein was highly expressed in hepatocellular carcinoma tissues, and might play an important role in the proliferation and invasion of human hepatoma HepG2 cells through epithelial-mesenchymal transition. Key words: Carcinoma, hepatocellular; Lysine-specific demethylase 1; Proliferation; Invasion; Epithelial-mesenchymal transition

Background: With rising demand for esthetic restorations, shade matching in dentistry blends art and scienceaffected by several external factors. While visual shade selection with a guide remains common for its simplicity, newer technologies like spectrophotometers and digital photography offer more objective, detailed color measurement and improved communication. Aim: The Aim of the study is to compare and evaluate the remineralizing ability of five different remineralizing agents on artificially created white spot lesions. Materials and Methods: Fifty maxillary premolars extracted for orthodontic purposes were taken for the study. Teeth are cleaned using ultrasonics and stored in 0.1 % thymol solution. 4 × 4 mm label placed on buccal surface of the crown. Remaining portion of tooth was painted with nail varnish. The samples were subjected to a demineralizing solution (0.264 gm of monosodium phosphate,0.244 gm of calcium chloride, 2.86 ml of acetic acid in 1l of deionised water) for 4 days to create an artificial white spot lesion. The samples are divided into 5 groups based on the remineralizing agent used. Group I : CPP-ACP; group 2: egg shell solution; group 3: red marine algae; group 4: bioactive glass nanoparticle solution; group 5: acclaim toothpaste. All the samples are sectioned to obtain the buccal cusp containing the experiment window and the EDAX analysis performed to obtain the ca, p and F values. The samples will be immersed in respective remineralising agents for 5 minutes, twice daily for a period of 6days and subjected to EDAX to obtain the ca p and f values after remineralization. The Ca, P and F values obtained after demineralization and remineralization will be compared. Results: Bioactive glass nanoparticle solution showed higher amount of remineralization compared to all the other study groups. The Ca, P and F values indicates the remineralization potential of all the study groups. Conclusion: Within the limits; the present study concluded that the Ca, P and F values of Bioactive glass nanoparticle solution have been increased significantly followed by Acclaim tooth paste, CPP ACP, Red marine algae and Egg shell solution

In this study we used pregabalin, diazepam and glucose for evaluating which one are more effective agents against glutamate induced neurotoxicity. After cortex culture preparation glutamate toxicity induced by adding 10-5 mM glutamate to each wells except negative control group. After 10 min different dose of diazepam, pregabalin and glucose were added for 24 h and then 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), Total Antioxidant Status (TAC) and Total oxidant status (TOS) assays were done. Diazepam, dose dependently shows neuroprotective effects and low dose of diazepam did not increase cell viability higher than 80 %. Combination groups only in high dose increased neurons viability. Total Antioxidant capacity of Pregabalin also show correlation with diazepam groups and highest dose of both show nearest value to negative control group. In addition, lowest and highest oxidant level were gained by the negative and positive control groups respectively. Except high dose of combination group, all groups show statically difference in compare with the negative control group P

This study aimed to compare the apical sealing of epoxy resin-based and mineral trioxide aggregate (MTA)-based rootcanal sealer. Twenty four permanent central incisors were selected at random and divided into 4 groups (N = 24),namely positive control group, negative control group, Fillapex obturator group (MTA, and TopSeal group (epoxyresin). The samples were decoronated, root canal preparation, then kept in incubator of 37 C for 24 hours. Sampleswere immersed in india ink for 7 days. The samples were washed with distilled water, dried and nail varnish removed.The samples was grooved longitudinally on both side, and then carefully sectioned. Penetration was measured using astereo microscope and given score 0-4. Measurements were analyzed statistically. By using the Mann Whitney andKolmogorov-Smirnov tests, there is no significant difference between the apical sealing of the epoxy resin root canalsealer with MTA root canal sealer based (p&gt;0.05). It means that the apical sealing of the epoxy resin root canal sealerbased comparable with MTA root canal sealer based. It was concluded that the apical sealing of epoxy resin-based sealer does not different to the MTA-based sealer.

Aims and Objectives:The aim of this study was to compare the in vitro efficacy of different remineralization (RM) agents on RM of artificial caries by evaluating the density, light reflection, and the crystal size of the newly formed mineral in primary and permanent teeth.Materials and Methods:Caries-free primary and permanent molars were divided into seven groups (n = 20) and treated with: G1 – Deionized water, G2 – Colgate Cavity Protection, G3 – Sensodyne Rapid Relief, G4 – GC MI Paste Plus, G5 – Clinpro Tooth Creme, G6 – Clinpro 5000, and G7 – Sensodyne Repair and Protect. Lesion depths were evaluated using laser fluorescence (DIAGNOdent), and polarized light microscopy (PLM), and the minerals were evaluated using X-ray diffractometry (XRD).Results:The highest decrease in laser fluorescence readings was observed in G6 for both primary and permanent teeth. No significant difference was observed between G2, G4, G5, and G6 in permanent teeth and G4 and G6 in primary teeth (P > 0.05). In PLM analyses, lesions depths were found to be lower in G6 compared to the other groups. No significant difference was observed between G2, G4, and G6 (P < 0.05). XRD evaluation showed that the newly formed mineral in G6 was denser and highly crystallized compared to the other groups.Conclusion:This in vitro study demonstrated that Clinpro 5000 is more efficient in remineralizing incipient enamel lesions compared to the other agents tested.