AI in cellular engineering and reprogramming

Background The use of artificial intelligence (AI) algorithms for the diagnosis of skin diseases has shown promise in experimental settings but has not yet been tested in real-life conditions. The COVID-19 pandemic led to a worldwide disruption of health systems, increasing the use of telemedicine. There is an opportunity to include AI algorithms in the teledermatology workflow. Objective The aim of this study is to test the performance of and physicians’ preferences regarding an AI algorithm during the evaluation of patients via teledermatology. Methods We performed a prospective study in 340 cases from 281 patients using patient-taken photos during teledermatology encounters. The photos were evaluated by an AI algorithm and the diagnosis was compared with the clinician’s diagnosis. Physicians also reported whether the AI algorithm was useful or not. Results The balanced (in-distribution) top-1 accuracy of the algorithm (47.6%) was comparable to the dermatologists (49.7%) and residents (47.7%) but superior to the general practitioners (39.7%; P=.049). Exposure to the AI algorithm results was considered useful in 11.8% of visits (n=40) and the teledermatologist correctly modified the real-time diagnosis in 0.6% (n=2) of cases. Algorithm performance was associated with patient skin type and image quality. Conclusions AI algorithms appear to be a promising tool in the triage and evaluation of lesions in patient-taken photographs via telemedicine. Conflicts of Interest None declared.

Trusted evidence. Informed decisions. Better health.

This article is devoted to the creation of a model of observational diagnostics for patients in dentistry using artificial intelligence as an information and measurement system. Artificial intelligence technology and algorithms in the field of clinical and radiographic image analysis in general dentistry and orthopedics have become the most common systems, contributing to accurate and timely diagnosis of a particular patient. Along with clinical practice images, radiographs are key tools for graphic visualization of the patient's dentition. A model of observational diagnostics for patients using artificial intelligence technology as the latest tool in the healthcare industry can become a structured method of information standardization and measurements in general dentistry. The model of observational diagnostics can serve as a fundamental component of functional diagnostics based on artificial intelligence algorithms, since they have a special potential for increasing the reliability and consistency of diagnostic measurements and information used by a dentist during treatment planning and restoration of the dentition. The model of observational diagnostics of dental patients presented by the authors implies a significant step forward in the development of highprecision methods of functional diagnostics, monitoring and analysis of patient condition measurements. The algorithm of the observational diagnostics model was integrated into the program, the operation of which is based on artificial intelligence technology in order to increase the efficiency, consistency, optimality and accuracy of the processes carried out in clinical practice. The purpose of the study is to determine the characteristics of the model of observational diagnostics of dental patients using artificial intelligence as an information and measuring system. Main results of the study: 1) the characteristics of the practical application of artificial intelligence technology for radiographs, clinical images of patients' teeth are provided; 2) the essential aspects of the model of observational diagnostics of dental patients, as well as the features of its practical application for planning dental treatment of patients are highlighted; 3) the results of practical testing of the model of observational diagnostics of dental patients are reflected (using the example of detection and treatment of caries). Research materials: the results of academic studies on the assessment of functional diagnostics of oral cavity and dentition pathologies using artificial intelligence technology; practical testing of the model of observational diagnostics for dental patients (using the example of detection and treatment of caries in a 49yearold woman). Research methods: systematization, comparative analysis, contrastive analysis, generalization, abstractlogical method, induction, deduction, systems approach, data conceptualization, graphical and tabular visualization of data, construction of informationmeasuring systems, artificial intelligence methods, study of clinical images and radiographs of patients' dental arches. The main conclusions of the study: 1) information and measuring instruments, the operation of which is based on artificial intelligence technology, have become the basis for the rapid processing of significant amounts of information, increasing the accuracy and efficiency of clinical decisions in dentistry. These information and measuring systems allow us to determine complex patterns on radiographs and clinical images of dental arches, to increase the degree of accuracy of functional diagnostics, in particular, in the presence of subtle pathologies and disorders; 2) artificial intelligence technology cannot replace a qualified dentist, such information and measuring systems only complement the accumulated human professional experience, allowing us to jointly carry out functional diagnostics of patients with a dental profile; 3) the potential of artificial intelligence technologies to create comprehensive plans and reports for dental treatment and dental restoration forms the basis for the joint activities of the latest tools and a dentist. In the context of the study, it is noted that artificial intelligence algorithms have become a powerful informationmeasuring tool for improving the decisionmaking process in dental practice, since digital technology promptly and quickly provides feedback to the dentist about missed anomalies or suboptimal methods of dental treatment and restorative medicine. Integration of the model of observational diagnostics for dental patients will make it possible to form an algorithm for the operation of artificial intelligence that fully complies with the recommendations for functional diagnostics and dental treatment of patients, developed on the basis of factual information. The use of artificial intelligence algorithms in dental practice will increase the efficiency of the general industry standard for providing dental services to patients.

Mesenchymal stem cells are found to have the potential to differentiate into many lineages, thus regulating diverse signalling cascades. This unique property of stem cells, called trans differentiation/linear reprogramming, aided in regenerative medicine and tissue repair. The mechanism of such regeneration is still unclear and requires further analysis. Due to the use of external or oncogenic factors, one of the approaches for mending cardiac, renal, and neurological disorders after an injury by induced pluripotent stem cells in the form of reprogramming does not show much benefit in the clinical setting. Consequently, cellular reprogramming may enable the application of clinical research to cell therapy, disease modelling, drug screening, and the fabrication of artificial organs. Studies related to this distinctive phenomenon of stem cells, where the cells could reprogramme themselves into completely different cell lineages, showed a promising future in therapeutic applications. However, unrelenting development in cellular reprogramming has prepared the ways for novel strategies in which signalling pathway manipulation may decide cellular destiny. This cellular reprogramming has got bright prospects in the field of regenerative medicine. Therefore, understanding the relationship between stochasticity and defining cell fate can help decipher molecular regulatory mechanisms of cellular reprogramming.

Artificial intelligence research started with computers trying to take the place of what humans have to do intelligently. Research started in the 1950s went through several ups and downs and reached the same period of the third boom as it is now. With the shocking situation where Alpha Go defeated Lee Se-dol, the strongest player in Go, in 2016, interest in artificial intelligence increased rapidly, and stories about the future situations that artificial intelligence will bring about, such as the possibility and singularity of artificial intelligence, are talked about even among the general public. Certainly, artificial intelligence has the potential to fundamentally change the way humans live, not just technological advances. The real value of artificial intelligence algorithms is prediction. At the center of all the algorithmic revolutions that have taken place in the various fields of modern society, there is one unchanging purpose: prediction. The algorithm for predicting the risk of recidivism used in US law enforcement fits precisely with the principle that individual humans behave in a predictable and consistent manner. While using the algorithm of predicting the risk of a second offender, an artificial intelligence algorithm, not a judge, actually takes the place of the defendant s decision on whether or not to be arrested or sentenced. Automated decision making continues to grow at an unprecedented rate and benefits because machine learning offers the possibility to extend the automated decision-making process, allowing wider, deeper decisions without human intervention. It is also very large and is expected to continue to expand its use. However, the risks are as great as the benefits of artificial intelligence algorithms. The risks of artificial intelligence algorithms outweigh the individual risks of any algorithmic technology specifically applied. Despite the widespread use of artificial intelligence in the social sphere, the reality is that the problems of artificial intelligence cannot be properly grasped. However, people who were treated differently due to automatic decision-making by artificial intelligence began to question the fairness or fairness of artificial intelligence, and in particular, while causing sensitive problems such as discriminatory results, controversy surrounding artificial intelligence algorithms gradually became more and more controversial. It is expanding. Even if a decision made by artificial intelligence is wrong or causes negative effects such as discrimination, there is a big problem that there are few means to object to this. In a situation where automated decision-making is more likely to threaten human autonomy and dignity, in order to secure an individual s autonomous personality, make sure that the entire process of the individual s automatic decision-making that affects him or her is fair and error-free. It is of utmost importance to know and allow humans to directly intervene if necessary. Although available data is increasing and algorithms are gradually improving, artificial intelligence algorithms do not completely eliminate the uncertainty posed by decision making. Eventually, there are cases in which humans, as finalists, have to make important decisions in situations where uncertainty has not been resolved. It is necessary to build a system in which artificial intelligence algorithms and human experts cooperate well to make the best decisions, and in addition to the direction of such a collaboration system establishment, the problem of legal responsibility due to errors in decision-making in artificial intelligence algorithms or collaboration systems is necessary. I want to deal with it in this paper.

BackgroundHighly accurate detection of intracranial hemorrhages (ICH) on head computed tomography (HCT) scans can prove challenging at high-volume centers. This study aimed to determine the number of additional ICHs detected by an artificial intelligence (AI) algorithm and to evaluate reasons for erroneous results at a level I trauma center with teleradiology services.MethodsIn a retrospective multi-center cohort study, consecutive emergency non-contrast HCT scans were analyzed by a commercially available ICH detection software (AIDOC, Tel Aviv, Israel). Discrepancies between AI analysis and initial radiology report (RR) were reviewed by a blinded neuroradiologist to determine the number of additional ICHs detected and evaluate reasons leading to errors.Results4946 HCT (05/2020-09/2020) from 18 hospitals were included in the analysis. 205 reports (4.1%) were classified as hemorrhages by both radiology report and AI. Out of a total of 162 (3.3%) discrepant reports, 62 were confirmed as hemorrhages by the reference neuroradiologist. 33 ICHs were identified exclusively via RRs. The AI algorithm detected an additional 29 instances of ICH, missed 12.4% of ICH and overcalled 1.9%; RRs missed 10.9% of ICHs and overcalled 0.2%. Many of the ICHs missed by the AI algorithm were located in the subarachnoid space (42.4%) and under the calvaria (48.5%). 85% of ICHs missed by RRs occurred outside of regular working-hours. Calcifications (39.3%), beam-hardening artifacts (18%), tumors (15.7%), and blood vessels (7.9%) were the most common reasons for AI overcalls. ICH size, image quality, and primary examiner experience were not found to be significantly associated with likelihood of incorrect AI results.ConclusionComplementing human expertise with AI resulted in a 12.2% increase in ICH detection. The AI algorithm overcalled 1.9% HCT.Trial registrationGerman Clinical Trials Register (DRKS-ID: DRKS00023593).

Idiopathic pulmonary fibrosis (IPF) is a chronic, progressive, and heterogeneous interstitial lung disease with a median survival of 2–5 years. Though the diagnosis has been improved due to newly published guidelines, the recognition of the prognosis of IPF remains a challenge. Recently, several studies attempted to build prognostic models by extracting predictive variates from pulmonary function data, basic information, or chest computed tomography (CT) and CT-derived parameters with clinical characteristics. Artificial intelligence (AI) algorithms, including principal component analysis, support vector machine, random survival forest, and convolutional neural network, could be applied to the procedure of IPF prognostic model, that is, region of interest extraction, image feature selection, clinical feature selection, and model construction. Compared to human visualization, AI algorithms show a higher efficiency in calculating and extracting deep features and a lower inter-observer variation. Thus, this review provides a comprehensive CT evaluation of IPF prognostic models and discusses the role of AI in constructing IPF prognostic models. The potential improvements of AI in CT assessments, including time-series CT analysis, optimization of AI algorithms, utilization of multi-modal data, and discovery of new biomarkers through unsupervised algorithms, could be introduced to make a more accurate and convenient assessment for the prognosis of IPF patients. This review describes the status quo and future direction of AI applications in CT analysis for prognostic models of IPF.Take home messageThe review summarizes the applications of CT and AI algorithms for prognostic models in IPF and procedures of model construction. It reveals the current limitations and prospects of AI-aid models, and helps clinicians to recognize the AI algorithms and apply them to more clinical work.

The convergence of edge computing with Artificial Intelligence (AI) constitutes a watershed point in technological growth, with transformational implications for a wide range of sectors. This chapter explores the mutually beneficial interaction between edge computing and AI, as well as the inherent difficulties and broad implications for the direction of computing in the future. With its decentralized processing model, edge computing puts data analysis closer to the point of origin, facilitating improved efficiency and real-time insights. Edge devices enable sophisticated cognitive processes and autonomous decision-making through seamless integration with AI algorithms. However, this integration creates architectural challenges that call for creative solutions to handle privacy and security issues and strike a balance between processing power and other resources. The chapter examines how edge computing-enabled AI can revolutionize manufacturing, transportation, healthcare, and smart cities. It shows how AI can change predictive maintenance, individualized healthcare monitoring, and urban infrastructure optimization. New advances offer avenues for collaborative applications and decentralized AI training. Federated learning models, the spread of edge AI chips and algorithms, and the democratization of AI capabilities are some of these themes. In summary, this chapter provides direction on navigating the rapidly evolving area of artificial intelligence (AI) facilitated by edge computing, encouraging collaboration and innovation to fully achieve AI's transformative potential and address digital age concerns.

Evaluation of Atrial Fibrillation Risk and Outcome after Allogeneic Transplantation Using an Artificial Intelligence ECG Algorithm and Echocardiographic Parameters in an MDS Cohort

This review delves into the fascinating realm of microbial insights enabled by artificial intelligence (AI), unveiling the mysteries of the intricate gut environment. Research into the human microbiome has evolved due to the fast development of AI and Machine learning (ML). Never before have such novel avenues for individualized medical care and therapeutic therapies been available as a result of this. Our first stop is at software developed specifically for microbiome data analysis. Complex datasets can be accessed and valuable information extracted using AI algorithms and machine learning approaches. Next, we take a look at predictive modeling of gut microbial interactions. Here we see how AI can foretell the actions of microorganisms and their effects on host health and illness. Afterwards, we investigate the efficacy of AI in detecting microbe biomarkers, which are crucial indicators of gut health and potential dangers of disease. A disease's root cause can be identified and a treatment strategy developed using this innovative approach. We also delve into the realm of personalized microbiome analysis and demonstrate how AI may assist in making dietary and lifestyle adjustments that are most suited to each individual in order to enhance their health. The impact of AI extends beyond the realm of research and assessment and include the development of novel medications. Our focus is on the ways AI is assisting the hunt for novel probiotics and microbiome-based therapies, which could one day lead to the development of effective remedies for various medical conditions. However, although we anticipate AI's potential, we must equally consider the ethical considerations involved in studying microbiota. This paper highlights the significance of data protection, transparency, and bias reduction in ensuring the responsible and fair use of AI. We can maximize AI's potential without trampling on people's rights or exacerbating existing inequalities if we adhere to ethical guidelines and work to earn the public's trust. Finally, this study demonstrates the potential power of AI-driven microbiome discoveries. By being committed to ethical principles and vigilant in our pursuit of new challenges, we may advance microbiota research toward a future of data-driven, customized healthcare that utilizes AI as a valuable tool for optimal health and wellness.

The article explores the issue of the possible use of artificial intelligence (AI) algorithms in the field of game development. The increasing complexity of video games (game mechanics, diverse plots, game environments) necessitates the effective use of the latest AI technologies for game development. The aim of the work is to investigate the possibility of using algorithms in game development and gameplay. The hypothesis of the study is that the use of AI in game development (GameDev) can lead to a reduction in development costs. AI can be used to generate game environment landscape textures that adapt to the actions of real characters. Dialogues of background non-player characters (NPCs) and sound effects can be generated using AI. AI can generate NPCs whose behavior can change depending on the actions of real players. This creates an exciting gaming experience for players. This approach can reduce the cost and duration of production and positively affect the diversity of the gameplay. The article reviews and systematizes AI algorithms that can be used in game development, provides examples, and discusses the specifics and advantages of their use. For this purpose, a research methodology was used that combined the analysis of literary sources and conducting research based on information about the implementation of AI in GameDev. AI algorithms have great potential for effective implementation in GameDev, because their implementation helps to diversify the gameplay, provides a unique gaming experience for each player. The player receives significant freedom of action, various possible dynamically changing scenarios and dialogues with NPCs. AI can not only respond to the player's actions, but also change the environment to make the gameplay interesting and exciting for the player, regardless of the level of his progress in the game. The implementation of AI technologies in GameDev mostly shows good efficiency, but they require high qualifications of developers and consideration of risks in the process of its implementation.

A Comprehensive Review of AI Algorithms for Performance Prediction, Optimization, and Process Control in Desalination Systems.

Background As per the Global Burden of Disease Study (GBD) 2019, chronic obstructive pulmonary disease (COPD) and asthma had a significant global burden. COPD is the fourth leading cause of death in the world and the second leading cause of death and disability-adjusted life years (DALYs) in India. Pulmonary function tests (PFTs) are commonly used diagnostic tools. They include spirometry, body plethysmography, and diffusion capacity. In regions with limited resources, pulmonologists often only have access to spirometry. Additionally, PFT pattern interpretation is usually unreliable and subjective. Recent rapid advances in artificial intelligence (AI) algorithms can bridge the gaps. Objectives This study aims to compare the accuracy of the predictions made by AI algorithms with pulmonologists using limited clinical data and spirometry. It also examines the consistency and accuracy of pulmonologists' predictions based on the same information. Methodology Different AI algorithms were trained, and their accuracy was evaluated. Spirometry and limited clinical data from 440 patients were interpreted by an AI algorithm and eight senior pulmonologists. Accuracy, sensitivity, specificity, positive predictive value (PPV), and negative predictive value (NPV) were calculated for the different patterns. Results Approximately 60% of the cases involved male patients, and about 70% were between the ages of 21 and 60. The Fleiss's kappa was 0.46. While the accuracy of pulmonologists against the gold standard was 65.82%, the accuracy of the AI was 86.59%. Conclusions PFTs, when interpreted by pulmonologists with limited clinical and spirometry data, have lower accuracy and higher variability. AI algorithms can consistently produce high accuracy. Adopting such technology among clinicians, especially in resource-constrained regions, could be pivotal for offering quality healthcare. In addition, it will also help in getting rid of inter-observer variability.

Real-World Surveillance of FDA-Cleared Artificial Intelligence Models: Rationale and Logistics.

Purpose This study aims to investigate ethical consideration into the data quality management and biases for fairness in artificial intelligence (AI) algorithms. It looks at how biases in AI systems could make it difficult to maintain data quality control in medical travel and tourism. Design/methodology/approach The systematic review of the literature from January 2019 to March 2025 (72 out of 925 articles) considers a variety of keyword combinations used for data quality control in AI systems. The process of systematic literature review aids in identifying studies on data quality management for medical tourism and travel. Findings The results of the study show that data quality visibility should be increased, data rectification should be done consistently and data momentum should be managed. It indicates that audience behavior in medical travel and tourism is significantly impacted by AI-powered digital branding and marketing. Research limitations/implications The research is limited to the single source of methodology, and it is limited in terms of articles collected from Jan 2019 to March 2025. It can be extended to the last decade for more insights and issues in AI use for medical travel and tourism. Practical implications Practical applications include efficient patient processing for international medical travel and tourism, as well as automated AI machine learning. Future research investigations should consistently segment data evaluation to overcome the limited regulatory compliance of data quality management. Social implications Patients (medical tourism) are primary elements in healthy social settings that can be facilitated through AI’s efficient use and maintaining the quality of the data management. Originality/value This study aims to address the main ethical concerns about the quality of AI data, data use monitoring and ethical support for data decisions and implementations inside the AI framework. The usage of digital branding, mobile app development, digital audience behavior, digital marketing principles and artificial intelligence in marketing are some of the important concepts used to accomplish the research goals.