AOAC SMPR® 2015.007

AOAC Standard Method Performance Requirements (SMPRs ) describe the minimum recommended performance characteristics to be used during the evaluation of a method. The evaluation may be an on-site veri cation, a single-laboratory validation, or a multi-site collaborative study. SMPRs are written and adopted by AOAC stakeholder panels composed of representatives from industry, regulatory organizations, contract laboratories, test kit manufacturers, and academic institutions. AOAC SMPRs are used by AOAC expert review panels in their evaluation of validation study data for method being considered for Performance Tested Methods or AOAC O cial Methods of Analysis , and can be used as acceptance criteria for veri cation at user laboratories.

AOAC SMPRs describe the minimum recommended performance characteristics to be used during the evaluation of a method. The evaluation may be an on-site veri cation, a single-laboratory validation, or a multi-site collaborative study. SMPRs are written and adopted by AOAC stakeholder panels composed of representatives from industry, regulatory organizations, contract laboratories, test kit manufacturers, and academic institutions. AOAC SMPRs are used by AOAC expert review panels in their evaluation of validation study data for methods being considered for Performance Tested Methods or AOAC O cial Methods of Analysis , and can be used as acceptance criteria for veri cation at user laboratories.

AOAC SMPRs describe the minimum recommended performance characteristics to be used during the evaluation of a method. The evaluation may be an on-site veri cation, a single-laboratory validation, or a multi-site collaborative study. SMPRs are written and adopted by AOAC stakeholder panels composed of representatives from the industry, regulatory organizations, contract laboratories, test kit manufacturers, and academic institutions. AOAC SMPRs are used by AOAC expert review panels in their evaluation of validation study data for methods being considered for Performance Tested Methods or AOAC O cial Methods of Analysis , and can be used as acceptance criteria for veri cation at user laboratories.

Intended Use: Surveillance and Monitoring of Infant Formula and Adult/Pediatric Formula by Trained Technicians 1 Purpose AOAC SMPRs describe the minimum recommended performance characteristics to be used during the evaluation of a method. The evaluation may be an on-site veri cation, a single-laboratory validation, or a multi-site collaborative study. SMPRs are written and adopted by AOAC stakeholder panels composed of representatives from industry, regulatory organizations, contract laboratories, test kit manufacturers, and academic institutions. AOAC SMPRs are used by AOAC expert review panels in their evaluation of validation study data for method being considered for Performance Tested Methods or AOAC O cial Methods of Analysis , and can be used as acceptance criteria for veri cation at user laboratories.

Intended Use: Surveillance and Monitoring by Trained Technicians 1 Purpose AOAC SMPRs describe the minimum recommended performance characteristics to be used during the evaluation of a method. The evaluation may be an on-site veri cation, a single-laboratory validation, or a multi-site collaborative study. SMPRs are written and adopted by AOAC stakeholder panels composed of representatives from industry, regulatory organizations, contract laboratories, test kit manufacturers, and academic institutions. AOAC SMPRs are used by AOAC expert review panels in their evaluation of validation study data for method being considered for Performance Tested Methods or AOAC O cial Methods of Analysis , and can be used as acceptance criteria for veri cation at user laboratories.

1 Purpose AOAC SMPRs describe the minimum recommended performance characteristics to be used during the evaluation of a method. The evaluation may be an on-site veri cation, a single-laboratory validation, or a multi-site collaborative study. SMPRs are written and adopted by AOAC stakeholder panels composed of representatives from industry, regulatory organizations, contract laboratories, test kit manufacturers, and academic institutions. AOAC SMPRs are used by AOAC expert review panels in their evaluation of validation study data for methods being considered for Performance Tested Methods or AOAC O cial Methods of Analysis , and can be used as acceptance criteria for veri cation at user laboratories.

AOAC SMPRs describe the minimum recommended performance characteristics to be used during the evaluation of a method. The evaluation may be an on-site veri cation, a single-laboratory validation, or a multi-site collaborative study. SMPRs are drafted by AOAC working groups composed of representatives from industry, regulatory organizations, contract laboratories, test kit manufacturers, and academic institutions. Approved by AOAC, AOAC SMPRs may be used for method development, are used by AOAC expert review panels in their evaluation of validation study data for methods being considered for AOAC Performance Tested Methods or AOAC O cial Methods of Analysis and can be used as acceptance criteria for veri cation at user laboratories.

AOAC SMPRs describe the minimum recommended performance characteristics to be used during the evaluation of a method. The evaluation may be an on-site verification, a single-laboratory validation, or a multi-site collaborative study. SMPRs are written and adopted by AOAC stakeholder panels composed of representatives from the industry, regulatory organizations, contract laboratories, test kit manufacturers, and academic institutions. AOAC SMPRs are used by AOAC expert review panels in their evaluation of validation study data for method being considered for Performance Tested Methods or AOAC Official Methods of Analysis , and can be used as acceptance criteria for verification at user laboratories.

Barcoding animal species

A single-laboratory validation of a method using Folin & Ciocalteu's phenol reagent (Folin-C reagent) for determination of total phenolic content of selected dietary supplement extracts was performed. The method is composed of a water extraction of dried extracts with sonication followed by reaction with the Folin-C reagent. The resulting colorimetric reaction is measured at 765 nm and compared with a standard curve generated with gallic acid standard solutions. The validation results were compared with Standard Method Performance Requirement (SMPR®) 2015.009, developed by the Stakeholder Panel on Dietary Supplements. The method demonstrated acceptable within-day RSDr of 1.96-7.47% for the five matrixes studied (grape seed extract, grape skin extract, black tea extract, green coffee extract, and cocoa extract). When gallic acid was spiked into maltodextrin (a surrogate dietary supplement carrier) at 30 or 70%, the recovery ranged from 91 to 104%, within the acceptable range established by SMPR 2015.009. Selectivity testing with glucose, fructose, and sucrose demonstrated no positive interference by these compounds. Finally, ruggedness studies demonstrated no significant effects due to changes in the heating apparatus, test material weight, read time after reaction, amount of Folin-C reagent, reaction time, reaction temperature, and amount of Na2CO3. The single-laboratory validation results support adoption of the method as First Action Official MethodSM 2017.13 and further evaluation in a collaborative study.

AOAC SMPRs describe the minimum recommended performance characteristics to be used during the evaluation of a method. The evaluation may be an on-site verification, a single-laboratory validation, or a multi-site collaborative study. SMPRs are written and adopted by AOAC through its stakeholder-based integrated science programs and projects, which are composed of representatives and experts from the academic, government, industry, and nonprofit sectors. AOAC SMPRs are used by AOAC method review experts, including expert review panels, in their evaluation of validation study data for methods being considered for AOAC Performance Tested Methods , Reviewed and Recognized , or AOAC Official Methods of Analysis , and can be used as acceptance criteria for verification at user laboratories.

What: AOAC Standard Method Performance Requirements (SMPRs) are voluntary consensus standards developed in accordance with the AOAC policy, "AOAC Due Process for Development of AOAC Non-Method Consensus Standards and Documents." SMPRs describe a scientific community's recommended minimum method performance characteristics and analytical requirements for a specific method related intended use.

AOAC SMPR® 2011.006.

AOAC SMPR 2016.014.

AOAC SMPR 2016.013.