Appendix E: HPLC Troubleshooting Quick Reference

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This section is designed to assist in troubleshooting system problems.It is not a systematic approach as described in Chapter 10, which always yields better results.Keeping this in mind, I have listed a series of commonly seen problems, possible causes, and suggested treatments.Problem 1: No power.(Display does not light up on module or system.)Cause a: Not plugged in.Treatment: Check the plug at the socket and at the module.It may have worked loose or been unplugged accidentally.Cause b: No fuse, blown fuse, or incorrect fuse.Treatment: On a new system, make sure fuse(s) were installed.If variable voltage, make sure the selection card is correctly positioned.New systems are often shipped with fuses in a bag.If fuse is broken, replace it and contact service.Cause c: Not switched on.Treatment: Turn on the switch (from the front, try the upper right-hand side of the back plate).Someone may have been helping you to conserve energy.I always set my system up on a common surge protector with an on/off switch.That way I can turn everything on with one switch and protect against line surges and ground loops at the same time.Problem 2: Leaking frits, puddles on desk top, fountains of solvent.Cause a: Compression fittings not tight enough.Treatment: Tighten leaking fitting another 1/4 turn or until leak stops.(Leaks will be at the back of the fitting around the tubing.)