Extraction, Purification, and Characterization of Galactomannan From Algerian Carob Seeds
ABSTRACTThe aim of this study is the extraction, purification, and characterization of a locust bean gum (LBG) biopolymer extracted from the endosperm of Algerian locust bean seeds using the hot water extraction method. Once the seeds were swollen, the endosperm was separated, dried and finally ground, sieved, and purified. The physicochemical, structural, rheological, thermal, and morphological properties, as well as antimicrobial activity of purified locust bean gum (pLBG), were studied. After purification, the resulting LBG powder was white, smooth, light, odorless, and tasteless, with a yield of around 23.17%. Fourier transform infrared spectroscopy analysis of the gum confirms galactomannan structure, attesting to successful extraction, while x‐ray diffraction (XRD) and thermogravimetric analysis (TGA) revealed an amorphous structure and good thermal stability, respectively. Gum morphology examined by scanning electron microscopy (SEM) revealed a porous, fibrous structure. The antimicrobial activity of pure LBG demonstrated a significant effect on all pathogenic bacteria tested. After 24 h, baseline cultures (106 CFU/mL) were reduced by 51% for methicillin‐resistant Staphylococcus aureus, 39% for Pseudomonas aeruginosa, and 36% for Klebsiella pneumoniae. In addition, carob galactomannan had a more pronounced effect on the pathogenic yeast Candida albicans, whose growth was reduced by 75.5%. Pure LBG has shown promising potential as an antibacterial agent, so it can be used in the food and pharmaceutical industries.
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