A gender difference in choice reaction time (RT) has been reported in the literature (e.g., Adam et al., 1999; Noble et al., 1964). Noble, Baker and Jones found that females had slower choice RT than their male counterparts many years ago. Later study by Adam et al. also reported a faster choice RT for male participants. Since Weiss (1965) introduced the measurement of RT components with electromyography (EMG), pre-motor time (PMT) and motor time (MT) have been used to identify the central (cognitive) and peripheral (neuromuscular) processing of the initiation of voluntary movement in human performance research. PURPOSE: To examine whether choice reaction time and its fractionated components differ between genders and dominant and non-dominant hands. METHODS: Twenty right-hand dominant college students (10 male, 26.0 ± 3.06 yrs; 10 female, 25.4 ± 2.27 yrs) were tested for their visual-manual choice reaction time for four consecutive days using both left and right hands. Based on the EMG activity of the pronator teres and biceps brachii, fractionated RTs of pronation and supination were measured for each hand. The learning effect was identified and a reliable measure was obtained. A 2 (gender) by 2 (hand) two-way mixed design analysis of variance (ANOVA) was used to analyze possible gender and hand difference as well as their interaction. RESULTS: Choice RT decreased across days (p < .05), however, no significant change was found between days 3 and day 4. No Gender x Hand interaction or hand difference was observed for choice RT. A significant gender difference was observed for total choice RT (F1,18 = 6.43, p < .05). The mean choice RT for the males was 278 ms, and for the females it was 308 ms. A further analysis of PMT and MT indicated that male participants had a faster PMT (218 ms) than female (246 ms) participants (F1,18 = 6.51, p < .05), but no MT difference was observed between the gender groups. CONCLUSIONS: There is no choice reaction time difference between dominant and non-dominant hands for the college students. Male participants demonstrated a faster choice RT than female participants, and the difference in total choice RT can be attributed to the PMT component, but not MT. The finding supports the proposed explanations that males might have employed a binary strategy while females used a serial strategy in choice reaction time tasks.