Classification of Blood Cells with Convolutional Neural Network Model

Understanding the learning mechanism of convolutional neural networks in spectral analysis

Land-cover and land-use classification from aerial images is a challenging problem due to high intra-class diversity and inter-class similarities of the images. To analyze the performances of deep convolutional neural network (CNN) models in this domain, we provide three pre-trained CNN models that are adapted to NWPU-RESISC45 dataset using three different training splits, i.e., 80%, 20%, and 10% ratios. The architectures of all three models are redesigned to be modest in size and their structure is kept simple, yet when tested with the NWPU-RESISC45 dataset, all three models perform comparably to the state-of-the-art models. Each of these models is then used to classify the scenes taken from five well-known datasets in this domain without any fine-tuning. We aim to assess the generalization capabilities of these models on the selected datasets. For better analysis, we considered using top-3 and top-5 accuracies of the models in addition to the best predicted category (top-1) that is usually reported by the models. This way of interpretation is very suitable in this domain, since the datasets contain a high number of fine grained categories with large semantic overlaps. We empirically show that the proposed CNN models actually learn the relevant semantic features in the aerial images better than we observed via standard measures. To the best of our knowledge, this is the first work in this domain that analyzes and presents model generalization performances the way we presented it here.

This study leveraged Convolutional Neural Network (CNN) models to estimate canopy height in Southeast Australian forests before and after the 2019–2020 bushfire event, using inputs from Sentinel-1, Sentinel-2, spectral indices, and terrain features and GEDI canopy height (rh99) as target variables. Our research consisted of three primary objectives: (1) an evaluation of GEDI rh99 canopy height in relation to an nDSM derived from airborne LiDAR, (2) the development of two CNN models for pre- and post-fire scenarios, and (3) utilizing the trained CNN models to generate pre- and post-fire canopy height maps and canopy height change maps across the continuous landscape. GEDI rh99 accuracy analysis revealed R 2 = 0.85, RMSE = 7.25 m and nRMSE = 0.23 for pre-fire GEDI and R 2 = 0.78, RMSE = 10.1 m and nRMSE = 0.3 for post-fire GEDI. The pre-fire CNN model achieved R 2 = 0.75, RMSE = 7.29 m and nRMSE = 0.22, and post-fire CNN model achieved R 2 = 0.62, RMSE = 11.67 m, and nRMSE = 0.34 when validated against nDSM. Bias analysis was conducted, revealing that GEDI rh99 underestimated nDSM across all canopy height ranges, and slope has no significant impact on GEDI accuracy. Notably, post-fire GEDI rh99 exhibited significant underestimation in high to extreme fire severity. The CNN models’ prediction displayed overestimation in short forests < 20 m and increasing underestimation in taller forests > 20 m and underestimation in high and extreme fire severity. Subsequent investigation indicated that canopy layer thinning in high fire severity conditions resulted in weak GEDI waveform signals and consequent bias. Additionally, the GEDI rh99 bias was propagated to the CNN models, together with insensitivity of spectral band values to canopy height in dense and burnt forests potentially contributed to models’ bias. Finally, we demonstrated CNN models’ ability to monitor forest recovery by generating canopy height maps spanning East Gippsland from 2019 to 2023.

Thousands of women worldwide are diagnosed with breast cancer yearly, which may be fatal if not treated. The diagnosis of the condition may take years, by which time the patient has little choice except to have the affected breast removed. Early diagnosis and treatments are the best ways to stop this disease's spread. In this study, the authors presented a Computer Aided Diagnosis (CAD) system to assist in breast cancer diagnosis. The study uses the Wisconsin breast cancer dataset to classify benign and malignant data. For the classification, three pre-trained Deep learning algorithms: Convolutional Neural Network (CNN), Long Short-Term Memory (LSTM), Multilayer Perceptron (MLP), were used. A novel CNN model that exceeds the performance efficiency of three pre-trained models and requires minimal compilation time is proposed. A number of evaluation matrices are used to analyze the models' classification abilities. Upon closer inspection, it has been established that the proposed CNN model outperforms CNN, LSTM, and MLP models with validation accuracy of 97.85%. CNN and LSTM performed with accuracies of 94.12% with the Adagrad optimizer and 93.5% with the Adam optimizer, respectively. Furthermore, MLP performance with 92.44% accuracy using the Adam optimizer. The proposed CNN model achieves the lowest Loss value and compilation time. In addition, the models' recall value, precision, and f1-score are computed to pick out the most effective model for diagnosing breast cancer on numeric data.

Purpose: Parkinson’s disease (PD) diagnosis algorithms based on Quantitative Susceptibility Mapping and image algorithms rely on SN labeling. However, the difference between SN labels from different experts (or segmentation algorithms) will have a negative impact on downstream diagnostic tasks, such as the decrease of the algorithm's accuracy or different diagnostic results for the same sample. In this article, we quantify the accuracy of the algorithm on different label sets, and then improve the Convolutional Neural Network Model to obtain a high-precision and high-robust diagnosis algorithm. Methods: The logistic regression model and convolutional neural network (CNN) model were first compared for classification between PD patients and healthy controls (HC), given different sets of substantia nigra (SN) labeling. Then, based on the CNN model with better performance, we further proposed a novel "Gated Pooling" operation and integrated it with deep learning to attain a joint framework for image segmentation and classification. Results: The experimental results show that, with different sets of SN labeling that mimic different experts, the CNN model can maintain the stable classification accuracy around 86.4%, while the conventional logistic regression model yields a large fluctuation ranging from 78.9% to 67.9%. Furthermore, the "Gated Pooling" operation after being integrated for joint image segmentation and classification can improve the diagnosis accuracy to 86.9% consistently, which is statistically better than the baseline. Conclusion: The CNN model, compared with the conventional logistic regression model using radiomics features, has better stability in PD diagnosis. Furthermore, the joint end-to-end CNN model is shown to be suitable for PD diagnosis from the perspectives of accuracy, stability, and convenience in actual use.

Automatic prediction of citrus fruit diseases requires the selection of a suitable classification model among various Convolutional Neural Network (CNN) models. The CNN models are the most effective for image classification. However, more than one CNN model occasionally performs closer to each other. Choosing the best classification model requires considerable effort in this situation. In this study, 17 CNN models were used for citrus fruit disease identification. Citrus fruit diseases include anthracnose, blackspot, canker, scab, and healthy. In this research, the performance of CNN models was analysed, and a two-phase statistical analysis was carried out to determine which CNN model provided the most accurate predictions of citrus crop disease. The 17 CNN models used were AlexNet, Darknet, Densenet, efficientnet, nasnetlarge, googlenet, inceptionresnetv2, inceptionv3, mobilenetv2, nasnetmobile, Xception, squeezenet, resnet18, resnet50, resnet101, vgg16, and vgg19. The two-phase statistical analysis was Duncan's multiple range test, followed by the Wilcoxon signed-rank test is the second one. Again, in order to do a statistical analysis, nine different indicators, including “accuracy, sensitivity, specificity, precision, FPR, F1 Score, MCC, Kappa, and computing time”, were taken into consideration. After the execution of 17 CNN models and twophase statistical analysis, it was revealed that resnet101 is superior among CNN models for citrus fruit disease prediction.

To better understand and improve the prediction of the seasonal surface temperature (TS) across the United States, we employed convolutional neural network (CNN) models trained on the Community Earth System Model Version 2 Large Ensemble (CESM2 LENS). We used lagged sea surface temperatures (SST) over the tropical Pacific region, containing the information of the El Niño Southern Oscillation (ENSO), as input for the CNN models. ENSO is the principal driver of variability in seasonal US surface temperatures (TSUS) and employing CNN models allows for spatiotemporal aspects of ENSO to be analyzed to make seasonal TSUS predictions. For predicting TSUS, the CNN models exhibited significantly improved skill over standard statistical multilinear regression (MLR) models and dynamical forecasts across most regions in the US, for lead times ranging from 1 to 6 months. Furthermore, we employed the CNN models to predict seasonal TSUS during extreme ENSO events. For these events, the CNN models outperformed the MLR models in predicting the effects on seasonal TSUS, suggesting that the CNN models are able to capture the ENSO‐TSUS teleconnection more effectively. Results from a heatmap analysis demonstrate that the CNN models utilize spatial features of ENSO rather than solely the magnitude of the ENSO events, indicating that the improved skill of seasonal TSUS is due to analyzing spatial variation in ENSO events. The proposed CNN model demonstrates a promising improvement in prediction skill compared to existing methods, suggesting a potential path forward for enhancing TSUS forecast skill from subseasonal to seasonal timescales.

BackgroundIdentification of lung cancer subtypes is critical for successful treatment in patients, especially those in advanced stages. Many advanced and personal treatments require knowledge of specific mutations, as well as up- and down-regulations of genes, for effective targeting of the cancer cells. While many studies focus on individual cell structures and delve deeper into gene sequencing, the present study proposes a machine learning method for lung cancer classification based on low-magnification cancer outgrowth patterns in a 2D co-culture environment.MethodsUsing a magnetic well plate holder, circular pattern lung cancer cell clusters were generated among fibroblasts, and daily images were captured to monitor cancer outgrowth over a 9-day period. These outgrowth images were then augmented and used to train a convolutional neural network (CNN) model based on the lightweight TinyVGG architecture. The model was trained with pairs of classes representing three subtypes of NSCLC: A549 (adenocarcinoma), H520 (squamous cell carcinoma), and H460 (large cell carcinoma). The objective was to assess whether this lightweight machine learning model could accurately classify the three lung cancer cell lines at different stages of cancer outgrowth. Additionally, cancer outgrowth images of two patient-derived lung cancer cells, one with the KRAS oncogene and the other with the EGFR oncogene, were captured and classified using the CNN model. This demonstration aimed to investigate the translational potential of machine learning-enabled lung cancer classification.ResultsThe lightweight CNN model achieved over 93% classification accuracy at 1 day of outgrowth among A549, H460, and H520, and reached 100% classification accuracy at 7 days of outgrowth. Additionally, the model achieved 100% classification accuracy at 4 days for patient-derived lung cancer cells. Although these cells are classified as Adenocarcinoma, their outgrowth patterns vary depending on their oncogene expressions (KRAS or EGFR).ConclusionsThese results demonstrate that the lightweight CNN architecture, operating locally on a laptop without network or cloud connectivity, can effectively create a machine learning-enabled model capable of accurately classifying lung cancer cell subtypes, including those derived from patients, based upon their outgrowth patterns in the presence of surrounding fibroblasts. This advancement underscores the potential of machine learning to enhance early lung cancer subtyping, offering promising avenues for improving treatment outcomes in advanced stage-patients.

Accurately identifying epileptogenic zone (EZ) using high-frequency oscillations (HFOs) is a challenge that must be mastered to transfer HFOs into clinical use. We analyzed the ability of a convolutional neural network (CNN) model to distinguish EZ and non-EZ HFOs. Nineteen medically intractable epilepsy patients with good surgical outcomes 2 years after surgery were studied. Five-minute interictal intracranial electroencephalogram epochs of slow-wave sleep were selected randomly. Then 5 s segments of ripples (80–200 Hz) and fast ripples (FRs, 200–500 Hz) were detected automatically. The EZs and non-EZs were identified using the surgery resection range. We innovatively converted all epochs into four types of images using two scales: original waveforms, filtered waveforms, wavelet spectrum images, and smoothed pseudo Wigner–Ville distribution (SPWVD) spectrum images. Two scales were fixed and fitted scales. We then used a CNN model to classify the HFOs into EZ and non-EZ categories. As a result, 7,000 epochs of ripples and 2,000 epochs of FRs were randomly selected from the EZ and non-EZ data for analysis. Our CNN model can distinguish EZ and non-EZ HFOs successfully. Except for original ripple waveforms, the results from CNN models that are trained using fixed-scale images are significantly better than those from models trained using fitted-scale images (p < 0.05). Of the four fixed-scale transformations, the CNN based on the adjusted SPWVD (ASPWVD) produced the best accuracies (80.89 ± 1.43% and 77.85 ± 1.61% for ripples and FRs, respectively, p < 0.05). The CNN using ASPWVD transformation images is an effective deep learning method that can be used to classify EZ and non-EZ HFOs.

The main goal of this research was to accurately diagnose diseases in pepper plants using convolutional neural network (CNN) models and visual parameters features. Thus, we aimed to build a computing system that can utilise visual cues to identify plant diseases with almost 100% reliability. A total of 2,200 images were trained and tested on a dataset, which was divided into healthy leaves and those with bacterial ailments. The images were preprocessed, resized to 300x300 pixels, and then segmented to extract the relevant traits from them. This CNN model, in a word, is accurate because it detects diseases with 99.65% accuracy. If we want to speak of a high accuracy rate, that's the CNN model. It learns to identify the intricate patterns and features in pepper plants that are associated with diseases. CNN can make revolutionary changes to the identification of plant diseases and help prevent crop damage through timely treatments. Automatic identification of the disease makes life easier for both agriculturists and scientists. The results underscore the importance of image preprocessing, segmentation, and feature extraction in improving the ability of CNN models to locate diseases. Additionally, the performance of the CNN model was compared with other machine learning models such as KNN, Decision Tree, ANN, and Random Forest. It was found that the developed CNN model can predict the response with greater accuracy than the others. Modern high-tech precision farming systems might utilise proposed CNN models for plant disease diagnosis.

Apple leaf diseases can cause significant crop failure and impact the economy of farmers and the agricultural industry. With the increasing demand for quality apples, it is important to develop effective and efficient solutions to detect apple plant diseases early. This research aims to develop an automated system that can identify diseases in apple plants based on leaf images using the Convolutional Neural Network (CNN) model. This model was developed with the ResNet50V2 architecture to classify four leaf conditions: three types of common diseases and one healthy condition. This research applies the CNN model for leaf image processing and the Waterfall system development method. The stages start from needs analysis by collecting data to be processed by the cnn model, interface design of the classification system, program code implementation, and functionality testing using black-box testing. CNN model development includes the stages of collecting datasets sourced from Malang apple plantations as many as 150 images and Kaggle public datasets totalling 3,071 images, then image preprocessing, model development and training. Our research results produced an apple plant disease classification system by implementing the CNN model. Based on the results of testing the system and the model used, it shows that the CNN model applied in the system achieves a classification accuracy of 99.01%, and the functionality of the system built runs well.

Rice is the staple food in Bangladesh. Every year many crops are lost because of the disease of the rice plant. Our farmers are facing a great loss every year as well as rice productivity is declining. Some diseased and healthy leaf images of rice plants have been collected. The images have been classified into four classes. The classes are Brown Spot, Hispa, Leaf Blast diseased leaves, and Healthy leaves, usually known as Oryza Saiva diseases of rice. Deep learning is very common and popular to analyze and make predictions from these kind of image data. In this research, the CNN (Convolutional Neural Network) models have been used to predict the classes. Among various CNN models, VGG16, MobileNet, ResNet50, and DenseNet121 pre-trained models have been applied. Among those pre-trained models, the highest accuracy 95.3% has been found from the VGG16 model. In addition, an Android mobile app has been developed using the highest performant VGG16 trained model. Using this mobile app, farmers will be able to upload a photo and predict the disease of that rice plant or not in a convenient way.

Dengue hemorrhagic fever (DHF) is a public health problem in Indonesia whose number of cases continues to increase and its spread is increasingly widespread.Tests used to diagnose dengue fever include a white blood cell count test.White blood cells are cells that play a role in the body's defense system. The reason is, white blood cells help fight infections in the body, such as viruses, bacteria, parasites and fungi. In patients infected with dengue fever, the white blood cell count is usually normal but then decreases (leukopenia) and neutropenia becomes dominant. Thisresearch was conducted from March to April 2023.The aim of this study was to measure the number of white blood cells in dengue fever patients.This type of research is descriptive. The research was carried out using an automatic analysis method (blood analysis) in the laboratory ofKlinik Dr.Trisna Garut, using samples from 30 dengue fever patients who were tested. In this study, the number of white blood cells in dengue fever patients at Klinik Dr.Trisna Garut was 14 (46.7%).Wich is Normal And the white blood cell count results of dengue fever patients at Klinik Dr.Tisna Garut General Hospital decreased by 16 (54.3%).

BACKGROUND AND AIM: Outdoor ultrafine particles (UFP; 100 nm) and black carbon (BC) vary greatly within cities and may have adverse impacts on human health such as cardiovascular mortality and brain tumour incidence. Traditional regression methods for estimating spatial distributions of ambient air pollution depend on extensive, curated geospatial information system databases. This has resulted in a disparity wherein little is known about local air pollution in the data-sparse settings of low- and middle- income countries. We used a hybrid approach to develop new models to estimate within-city spatial variations in outdoor UFP and BC concentrations across Bucaramanga, Colombia. METHODS: We conducted a large-scale mobile monitoring campaign over twenty days in 2019. Land use regression (LUR) models were developed using land use parameters from curated and open-source databases. Convolutional Neural Network (CNN) models were developed using Google Maps satellite view and street view images. Combined models were developed by combining predictions from the LUR and CNN models. After training in the training and validation sets, predictions were generated in the test set and compared to measured values. RESULTS:The combined UFP model (R2=0.54) outperformed the CNN (R2=0.47) and land use regression (LUR) models (R2=0.47) on their own. Similarly, the combined BC model also outperformed the CNN and LUR BC models (R2 = 0.51 vs 0.43 and 0.45 respectively). Spatial variations in model performance were more stable for the CNN and combined models compared to the LUR models suggesting that the combined approach may be less likely to contribute to differential exposure measurement error in epidemiological studies. CONCLUSIONS:Estimates from these models can then be applied to population-based cohorts in order to evaluate population health risks. Additionally, our findings demonstrated that satellite and street-level images can be combined with a traditional LUR modelling approach to improve predictions of within-city spatial variations in outdoor UFP and BC concentrations. KEYWORDS: Ultrafine particles, Black Carbon, Deep learning, Images, Land use regression

Among the many deep learning methods, the convolutional neural network (CNN) model has an excellent performance in image recognition. Research on identifying and classifying image datasets using CNN is ongoing. Animal species recognition and classification with CNN is expected to be helpful for various applications. However, sophisticated feature recognition is essential to classify quasi-species with similar features, such as the quasi-species of parrots that have a high color similarity. The purpose of this study is to develop a vision-based mobile application to classify endangered parrot species using an advanced CNN model based on transfer learning (some parrots have quite similar colors and shapes). We acquired the images in two ways: collecting them directly from the Seoul Grand Park Zoo and crawling them using the Google search. Subsequently, we have built advanced CNN models with transfer learning and trained them using the data. Next, we converted one of the fully trained models into a file for execution on mobile devices and created the Android package files. The accuracy was measured for each of the eight CNN models. The overall accuracy for the camera of the mobile device was 94.125%. For certain species, the accuracy of recognition was 100%, with the required time of only 455 ms. Our approach helps to recognize the species in real time using the camera of the mobile device. Applications will be helpful for the prevention of smuggling of endangered species in the customs clearance area.