Cloud Point Extraction and Spectrophotometric Determination Zinc II Ion Complex in Real Samples Using BTAD Reagent

Using natural plant extracts as food additives is a promising approach for improving food products' quality, nutritional value, and safety, offering advantages for both consumers and the environment. Therefore, the main goal of this study was to develop a sustainable method for extracting polyphenols and carotenoids from horned melon peel using the cloud point extraction (CPE) technique, intending to utilize it as a natural food additive. CPE is novel promising extraction method for separation and pre-concentration of different compounds while being simple, inexpensive, and low-toxic. Three parameters within the CPE approach, i.e., pH, equilibrium temperature, and equilibrium time, were investigated as independent variables through the implementation of Box-Behnken design and statistical analyses. The optimized conditions for the maximum recovery of both polyphenols and carotenoids, reaching 236.14 mg GAE/100 g and 13.80 mg β carotene/100 g, respectively, were a pH value of 7.32, an equilibrium temperature of 55 °C, and an equilibrium time of 43.03 min. The obtained bioactives' recovery values under the optimized conditions corresponded to the predicted ones, indicating the suitability of the employed RSM model. These results highlight the effectiveness of CPE in extracting bioactive compounds with varying polarities from agricultural by-products, underscoring its potential for enhancing the value of food waste and advancing sustainable practices in food processing. According to microbiological food safety parameters, the optimal CPE extract is suitable for food applications, while its storage under refrigerated and dark conditions is particularly beneficial. The CPE extract's enhanced stability under these conditions makes it a more viable option for long-term storage, preserving both safety and quality.

The purpose of the study was to identify a quick, simple and sensitive spectrophotometric technique for the assessment of salbutamol (SAL). The proposed approach depends on SAL’s azo-coupling reaction with BEN. The orange color of the reaction product was analyzed at λmax (480 nm) against a blank solution. It was obeyed to beer-lambert show over concentration between (2.5-17.5 mg.L−1) with LOD (1.283 mg.L−1), LOQ (4.234 mg.L−1) and molar absorptivity (12922.74 L.mol−1.cm−1). The procedure revealed high sensitivity for assessment of chosen drug. The new cloud point extraction technique was also successfully utilized for the extraction of pharmaceutically pure SAL drug, (also known as 2-[4-(2,4,4-trimethylpentan-2-yl) phenoxy] ethanol), was selected as green extraction solvent due to its properties and structure. The influence of various parameters, such as the kind and volume of surfactant, salt, temperature and incubation time on the CPE of salbutamol was investigated in detail and a set of perfect conditions was established. A correlation coefficient (R2) was 0.9914 for the calibration curve was obtained. The LOD, LOQ and molar absorptivity were 0.041 mg.L−1, 0.12 mg.L−1 and 47024.61 L.mol−1.cm−1, respectively. We believe that our suggested method is rapid, very convenient, and cost effective for the determination of salbutamol drug in the various samples.

Development of a precise and delicate reaction has been acquired for the determination of vancomycin hydrochloride using batch and cloud point extraction (CPE) methods. The first method is based on the formation of azo dye as a result of diazotized dapsone coupled with vancomycin HCl (VAN) in a basic medium. The sensitivity of this reaction was enhanced by utilizing a nonionic surfactant (Triton X-114) and the cloud point extraction technique (second method). The azo dye formed was extracted into the surfactant-rich phase, dissolved in ethanol and detected at λmax 440 nm spectrophotometrically. The reaction was investigated using both batch and CPE methods (with and without extraction), and a simple comparison between the two developed methods was made. The conditions that affect the extraction process and the sensitivity of the methods have been carefully examined. The linearity of the calibration curves was in the range of 3-50 and 0.5- 25 µg.mL-1 with limits of detection of 0.806 and 0.462 µg.mL-1 for VAN in both batch and CPE procedures, respectively. The percentage of relative standard deviation (R.S.D.%) for the two methods was better than 2.54% and 2.83%, respectively. The recommended procedures have been effectively used to assay VAN in commercial injections.

Comparison of preconcentration and determination methods of a textile dye by spectrophotometry: cloud point extraction and solid-phase extraction

In this work, cloud point extraction (CPE) technique was developed for the separation and pre-concentration of Cd(II). CPE was used with lipophilic hexadentate (N4O2) Schiff base ligand, L22pysa (1, C24H26N4O2). The methodology is based upon the formation of a Cd(II)/L complex soluble in a micellar phase the non-ionic surfactant Triton X-114. This complex is then extracted into the surfactant-rich phase above its cloud point temperature. Several important variables that affect the CPE were investigated and optimized. Under the optimum experimental conditions, the calibration graph was linear over the range 1–100 ng mL−1 with a correlation coefficient of 0.9997. The detection limit obtained under optimum conditions was 0.44 ng mL−l. The proposed method was successfully applied to the determination of Cd(II) in rice and various water samples.

Two simple, rapid, and useful spectrophotometric methods were suggest or the determination of sulphadimidine sodium (SDMS) with and without using cloud point extraction technique in pure form and pharmaceutical preparation. The first method was based on diazotization of the Sulphdimidine Sodium drug by sodium nitrite at 5 ºC, followed by coupling with α –Naphthol in basic medium to form an orange colored product . The product was stabilized and its absorption was measured at 473 nm. Beer’s law was obeyed in the concentration range of (1-12) μg∙ml-1. Sandell’s sensitivity was 0.03012 μg∙cm-1, the detection limit was 0.0277 μg∙ml-1, and the limit of Quantitation was 0.03605μg∙ml-1.The second method was the cloud point extraction (CPE) using Trtion X-114 as surfactant. Beer’s law was obeyed in the concentration range of (1-12) μg∙ml-1. Sandell’s sensitivity was 0.02958 μg∙cm-1, the detection limit was 0.01745 μg∙ml-1, and the limit of quantitation was 0.028303 μg∙ml-1. All variables including the reagent concentration, reaction time, color stability period, and mole ratio were studied in order to optimize the reaction conditions. The mole ratio for the composition of product is (1:1). Both methods were effectively useful to the determination of sulphdimidine sodium in pharmaceutical dose form. The attained results were in a good agreement with the official and other methods in the literature .No interference were observed from the commonly encountered additives and excipients.

Liquid-liquid extraction (LLE) and cloud point extraction (CPE) of vanadium(V) ternary complexes with 4-(2-pyridylazo)resorcinol (PAR) and 2,3,5-triphenyl-2H-tetrazolum chloride (TTC) were investigated. The optimal conditions for vanadium extraction and spectrophotometric determination were identified. The composition (V : PAR : TTC) of the extracted species was 1 : 2 : 3 (optimal conditions; LLE), 2 : 2 : 2 (low reagents concentrations; LLE), 1 : 1 : 1 (short heating time; CPE), and 1 : 1 : 1 + 1 : 1 : 0 (optimal extraction conditions; CPE). LLE, performed in the presence of 1,2-diaminocyclohexane-N,N,N’,N’-tetraacetic acid and NH4F as masking agents, afforded the sensitive, selective, precise, and inexpensive spectrophotometric determination of vanadium. The absorption maximum, molar absorptivity, limit of detection, and linear working range were 559 nm, 1.95 × 105 dm3 mol−1 cm−1, 0.7 ng cm−3, and 2.2-510 ng cm−3, respectively. The procedure thus developed was applied to the analysis of drinking waters and steels. The relative standard deviations for V(V) determination were below 9.4 % (4-6 × 10−7 mass %; water samples) and 2.12 % (1-3 mass %; steel samples).

Cloud Point Extraction of Bisphenol A from Water Utilizing Cationic Surfactant Aliquat 336

Development of a cloud point extraction and spectrophotometry-based microplate method for the determination of nitrite in human urine and blood

A fast, economical and efficient cloud point extraction (CPE) method is presented for the enrichment of copper (II) in aqueous samples. Hydrophobic chelate was formed in the surfactant rich phase after complexing copper(II) with 2- amino derivative-1,2,3-thiadiazole (R) in presence of Triton X-11 as micellar medium. The copper content was measured by uv-visible spectrometry after all experimental conditions were optimized for CPE such as pH, concentration for (R), concentration for Triton X-114, equilibration temperature, incubation time and centrifugation (rate and time). At the optimized conditions of a 5.0 mL sample gave the enrichment factor and Sandell's sensitivity (41.6, 5.5493x10-3µg.cm-2/0.001AU) respectively. The calibration curve linear was obtained in the range of (0.9-4.2) µg.mL−1 with a correlation coefficient of (0.9994) and molar absorptivity (ε=11450.9 L.mol-1.cm-1). Precision, expressed as relative standard deviation (R.S.D, n=7, 15.36 µg.mL−1 Cu (II) ) was 1.9 %, the limit of detection was (LOD=0.11 µg.mL−1) and Limit of quantitation (LOQ=0.388 µg.mL-1).

A new cloud-point extraction and preconcentration method using a cationic surfactant, Aliquat-336 (tricaprylylmethylammonium chloride), has been developed for the determination of cyanobacterial toxins, microcystins, in natural waters. Sodium sulfate was used to induce phase separation at 25 degrees C. The phase behavior of Aliquat-336 with respect to concentration of Na2SO4 was studied. The cloud-point system revealed a very high phase volume ratio compared to other established systems of nonionic, anionic, and cationic surfactants. At pH 6-7, it showed an outstanding selectivity in analyte extraction for anionic species. Only MC-LR and MC-YR, which are known to be predominantly anionic, were extracted (with averaged recoveries of 113.9 +/- 9% and 87.1 +/- 7%, respectively). MC-RR, which is likely to be amphoteric at the above pH range, was not detectable in the extract. Coupled to HPLC/UV separation and detection, the cloud-point extraction method (with 2.5 mM Aliquat-336 and 75 mM Na2SO4 at 25 degrees C) offered detection limits of 150 +/- 7 and 470 +/- 72 pg/mL for MC-LR and MC-YR, respectively, in 25 mL of deionized water. Repeatability of the method was 7.6% for MC-LR and 7.3% for MC-YR. The cloud-point extraction process can be completed within 10-15 min with no cleanup steps required. Applicability of the new method to the determination of microcystins in real samples was demonstrated using natural surface waters collected from a local river and a local duck pond spiked with realistic concentrations of microcystins. Effects of salinity and organic matter (TOC) content in the water sample on the extraction efficiency were also studied.

Species selective preconcentration and quantification of gold nanoparticles using cloud point extraction and electrothermal atomic absorption spectrometry

Salvia miltiorrhiza (SM) contains the tanshinones, a compound with various pharmacological effects, and has been extensively studied as a pharmaceutical material. However, conventional methods for extracting tanshinones face challenges such as environmental hazards and high cost. In this study, we aimed to effectively extract tanshinones from SM using cloud point extraction (CPE) with lecithin, a natural surfactant. By optimizing various extraction conditions including the solid-to-liquid ratio, lecithin concentration, NaCl concentration, pH, and equilibrium temperature, the optimal extraction efficiency was achieved using 20 mL of solvent per 1 g of sample, 3% lecithin (w/v), 2% NaCl (w/v), pH 6, and room temperature (25 ± 2 °C). The CPE method, which minimizes the use of organic solvent and is eco-friendly, demonstrated improvements in extraction efficiency, with a 4.55% increase for dihydrotanshinone I, 8.32% for cryptotanshinone, 15.77% for tanshinone I, and 6.81% for tanshinone IIA compared to the conventional water extraction method. These results suggest that CPE is a promising, environmentally friendly, and efficient approach for extracting hydrophobic components from pharmacologically active materials such as SM, with potential applications across various fields of natural product extraction.

New simple sensitive spectrophotometric methods are developed for the estimation of Sulfadoxine (SFD) in pure and pharmaceutical formulations.The first method includes a conversion primary amine to azo-dye by reacting sulfadoxine with sodium nitrite and hydrochloric acid followed by coupling with4-methoxyphenol in alkaline medium to obtain a stable reddish-orange colored dye at λmax495nm.Concentration ranges 0.25-60 μg / mL, obeyed Beer's law, correlation coefficient was 0.9996, molar absorptivity was 0.589×10 4 L.mol -1 .cm - and the detection limit was 0.157μg/mL.The second method was cloud point extraction (CPE) for estimating trace amount in an aqueous solution that produced from diazotization and measuring with a UV-visible spectrophotometer as are reddish-purple colored product at λmax500 nm.The concentration range obeyed the Beer's law was 0.25-6μg / mL, correlation coefficient was 0.9998, molar absorptivity was 0.877×10 5 L.mol -1 .cm - , detection limit was 0.023μg/mL, pre-concentration factor was 25 and Distribution coefficient(D) was 320.88.The last method was flow injection analysis it's simple for estimation the sulfadoxine.The concentration range was1-150μg / mL, obeyed Beer's law,the correlation coefficient was 0.9997, molar absorptivity was 0.273×10 4 L.mol -1 .cm - and the detection limit was 0.375μg/mL.The offered methods were successful, useful for estimating sulfadoxine in traditional medications

In the present study, an inexpensive, selective, and micellar sensitive spectrophotometric method for Sn(IV) was developed at 537 nm. The method is based on the formation of ternary complex of Sn(IV) with tartaric acid and Celestine blue in the presence of sodium dodecyl sulfate (SDS) and polyethylene glycol tert-octylphenyl ether (Triton X-114) at pH 3.5. The analytical variables affecting cloud point extraction (CPE) efficiency were optimized in detail. Under the optimized conditions, the calibration graph was linear in the range of 4–350 μg L−1 with changing dye concentration for Sn(IV) ions. The limits of detection and quantification of the method were found to be 1.3 and 4.3 μg L−1 for Sn(IV), respectively. Relative standard deviation (RSDs) for five replicate determinations at 20 and 100 μg L−1 concentration levels were 4.4 and 1.5 %, respectively. Recovery values of spiked samples were obtained in range of 97.7–103.3 %. Matrix effect on the extraction efficiency were also investigated. The method was successfully applied to the determination of Sn(II), Sn(IV) and total Sn in some selected beverages before and after pretreatment with H2O2 in acidic media, prior to analysis. Validity of the method was controlled through the analysis of two certified reference materials. It has been observed that the results are highly compatible with the certified values.