Combining frequency navigator and optical prospective motion correction for functional MRS during motor activation at 3T: A feasibility study.

Real-time multislice-to-volume motion correction for task-based EPI-fMRI at 7T.

IntroductionAnodal direct current stimulation (a-tDCS) of the visual cortex is a potential rehabilitation tool for vision disorders such as amblyopia and macular degeneration. However, the underlying neural mechanisms are currently unknown. When applied to the human motor cortex, a-tDCS reduces the concentration of gamma-aminobutyric acid (GABA), an inhibitory neurotransmitter that modulates neuroplasticity. Our primary aim was to assess whether the same a-tDCS paradigm alters local GABA concentration when applied to the healthy primary visual cortex. We also measured the effect of a-tDCS on visual cortex resting-state connectivity and sought to replicate reported observations of an association between visual cortex GABA concentration and the dynamics of binocular rivalry.MethodsFourteen participants with normal vision completed two brain imaging sessions at least 48 hours apart. In each session, binocular rivalry dynamics, primary visual cortex GABA and glutamate-glutamine (Glx) concentrations (via magnetic resonance spectroscopy (MRS)) and resting-state functional connectivity (via task-free fMRI) were measured at baseline. Real or sham a-tDCS (20 min, 2mA) was then applied to the visual cortex in a randomized sequence followed by a second set of MRS and fMRI measurements.ResultsNo between-session effects of a-tDCS on GABA or Glx concentration or resting-state functional connectivity were observed. A pre-planned within-session analysis revealed a significant increase in Glx following a-tDCS that did not withstand multiple comparisons correction. No consistent relationships between binocular rivalry dynamics and GABA concentration were apparent.DiscussionTogether, our results suggest that a-tDCS effects on the visual cortex may differ from the GABA-associated mechanism in motor cortex.

Increased Glutamate concentrations during prolonged motor activation as measured using functional Magnetic Resonance Spectroscopy at 3T

Recent functional magnetic resonance spectroscopy (fMRS) studies have shown changes in glutamate/glutamine (Glx) concentrations between resting-state and active-task conditions. However, the types of task used have been limited to sensory paradigms, and the regions from which Glx concentrations have been measured limited to sensory ones. This leaves open the question as to whether the same effect can be seen in higher-order brain regions during cognitive tasks. Cortical midline structures, especially the medial prefrontal cortex (MPFC), have been suggested to be involved in various such cognitive tasks. We, therefore set out to use fMRS to investigate the dynamics of Glx concentrations in the MPFC between resting-state and mental imagery task conditions. The auditory cortex was used as a control region. In addition, functional magnetic resonance imaging was used to explore task-related neural activity changes. The mental imagery task consisted of imagining swimming and was applied to a large sample of healthy participants (n = 46). The participants were all competitive swimmers, ensuring proficiency in mental-swimming. Glx concentrations in the MPFC increased during the imagery task, as compared to resting-state periods preceding and following the task. These increases mirror BOLD activity changes in the same region during the task. No changes in either Glx concentrations or BOLD activity were seen in the auditory cortex. These findings contribute to our understanding of the biochemical basis of generating or manipulating mental representations and the MPFC's role in this.

The present study evaluates the neuroprotective effect of α-lipoic acid (ALA) and/or metformin (MET) on the behavioral and neurochemical changes induced by hypothyroidism. Rats were divided into control, rat model of hypothyroidism induced by propylthiouracil, and rat model of hypothyroidism treated with ALA, MET, or their combination. Behaviorally, hypothyroid rats revealed impaired memory and reduced motor activity as indicated from the novel object recognition test and open-field test, respectively. Hypothyroidism induced a significant increase in lipid peroxidation (malondialdehyde [MDA]) and a significant decrease in reduced glutathione (GSH) and nitric oxide (NO) in the cortex and hippocampus. These were associated with a significant increase in tumor necrosis factor-α (TNF-α) and a significant decrease in brain-derived neurotrophic factor (BDNF). Hypothyroidism decreased significantly the levels of serotonin (5-HT), norepinephrine (NE), and dopamine (DA) and reduced the activities of acetylcholinesterase (AchE) and Na+, K+-ATPase in the cortex and hippocampus. Treatment of hypothyroid rats with ALA and/or MET showed an improvement in memory function and motor activity. Moreover, ALA and/or MET prevented the increase in MDA and TNF-α, and the decline in GSH, NO, BDNF, 5-HT, NE, and DA. It also restored AchE and Na+, K+-ATPase activities in the studied brain regions. ALA and/or MET has a potential neuroprotective effect against the adverse behavioral and neurochemical changes induced by hypothyroidism in rats.

The prefrontal cortex (PFC) plays an important role in cognitive function, involved in Executive Functions (EFs) such as planning, working memory, and inhibition. Activation in the PFC also occurs during some motor activities. One commonly used tool to assess EF is the Tower of Hanoi, demonstrating sensitivity to PFC dysfunction. However, limited neuroimaging evidence is available to support the contribution of the PFC in the Tower of Hanoi task. In the current study, we use functional near infrared (fNIR) spectroscopy to examine hemodynamic responses associated with neural activity in the PFC in adults as they participate in the Tower of Hanoi task. We compared changes in cerebral oxygenation during resting, a motor task (tapping), and the Tower of Hanoi in 16 neurotypical adults, with measures of relative changes in concentration of oxygenated hemoglobin (Δoxy-Hb) and deoxygenated hemoglobin (Δdeoxy-Hb) taken throughout tasks, as well as total hemoglobin (ΔHbT) and oxygenation (Δoxy). Performance on the Tower of Hanoi was measured by the number of moves used to complete each level and the highest level of successful performance (3, 4, or 5 disks). We found a significant higher value of Δoxy-Hb and Δoxy in dorsolateral PFC (DLPFC) during the Tower of Hanoi as compared to tapping and resting. Significant changes in Δdeoxy-Hb and ΔHbT during the Tower of Hanoi were found in the right DLPFC only. These results support the notion that the Tower of Hanoi task requires higher levels of PFC activity than a similar motor task with low executive function demands.

Blood Oxygen Level Dependent (BOLD) is a commonly-used MR imaging technique in studying brain function. The BOLD signal can be strongly affected by specific sequence parameters, especially in small field strengths. Previous small-scale studies have investigated the effect of TE on BOLD contrast. This study evaluates the dependence of fMRI results on echo time (TE) during concurrent activation of the visual and motor cortex at 1.5 T in a larger sample of 21 healthy volunteers. The experiment was repeated using two different TE values (50 and 70 ms) in counterbalanced order. Furthermore, T2* measurements of the gray matter were performed. Results indicated that both peak beta value and number of voxels were significantly higher using TE = 70 than TE = 50 ms in primary motor, primary somatosensory and supplementary motor cortices (p < 0.007). In addition, the amplitude of activation in visual cortices and the dorsal premotor area was also higher using TE = 70 ms (p < 0.001). Gray matter T2* of the corresponding areas did not vary significantly. In conclusion, the optimal TE value (among the two studied) for visual and motor activity is 70 ms affecting both the amplitude and extent of regional hemodynamic activation.

Arterial spin labeling (ASL) is a non-invasive MRI technique that allows the quantitative measurement of perfusion, (regional cerebral blood flow (rCBF)). The ASL techniques use the labeling of the blood, by inverting or saturating the spins of water molecules of the blood supplying the imaged region. When reaching the capillary bed, these will be exchanged with tissue water giving rise to a perfusion-weighted signal. The subtraction of control (without label) from labeled images yields a signal difference that directly reflects the local perfusion. Being a non-invasive method, it can be repeated as many times as needed allowing the brain perfusion variation quantification associated with endogenous and exogenous stimuli. In this study, the authors have evaluated the CBF variation induced by the neural activity during a common motor task. The study was conducted on a Siemens Verio 3T system using a 12-channel head coil and a pulsed ASL Q2TIPS-PICORE sequence with a GE-EPI readout. The sequences were driven in 3D PACE mode for prospective motion correction. Fifteen healthy volunteers were studied using a simple motor task consisting in sequential thumb-digit opposition. Two different functional ASL protocols were used: #1 one perfusion scan was obtained during rest and another one during an equal period of motor task (total scan time ~8 min) (TI1 = 700 ms, TI1s = 1600 ms, TI2 =1800 ms; 91 Interleaved tag and control volumes were acquired; TR/TE = 2500/25 ms and flip angle = 90°; nine contiguous axial slices of 8 mm thickness acquired in-line with the AC-PC axis, positioned from the vertex of the brain to the top of cerebellum; FOV = 256 × 256 mm(2); matrix 64 × 64; gap between the labeling slab and the proximal 18.8 mm) and #2 a block design alternating five 25s periods of motor task with five 25s periods of rest (total scan time ~4 min) (TI1 = 700 ms, TI1s = 1600 ms, TI2 = 1800 ms; 101 interleaved tag and control volumes were acquired; TR/TE = 2500/11 ms and flip angle = 90°; nine contiguous axial slices of 6 mm thickness acquired in-line with the AC-PC axis, positioned from the vertex of the brain to the top of cerebellum; FOV = 256 × 256 mm(2) ; matrix 64 × 64; gap between the labeling slab and the proximal 18.8 mm). The post-processing was performed using FSL (www.fmrib.ox.uk/fsl). The mean CBF values obtained for protocols #1 / #2 were: CBFrest = 61.0 / 69.4 ml/100g/min; CBFactivation = 104.8 / 109.9 ml/100g/min; and CBFvariation = CBFactivation - CBFrest = 43.7 / 40.5 ml/100g/min. The relative perfusion changes during activation [defined as CBFvariation / CBFrest (%)] were 73±6 % and 62±7 % (mean±SE) for protocols #1 and #2, respectively. These results show that both activation vs rest and block design functional protocols were capable to detect consistent variations in perfusion associated with a simple motor task. However, the block design has the advantages of requiring shorter acquisitions, directly comparing rest and activation conditions and allowing the acquisition of simultaneous Blood oxygenation level dependent (BOLD) contrast information, while still providing comparable results with the more conventional activation vs rest protocol. In conclusion, our results indicate that a block design ASL-BOLD protocol may be a preferable approach for the evaluation of perfusion changes to endogenous stimuli.

Developmental exposure to low level ambient ultrafine particle air pollution and cognitive dysfunction

Previous studies have shown that performing a motor action toward a target decreases the perceptual asynchrony observed in a temporal order judgment (TOJ) of a change in the target's visual attributes. We examined the temporal limit of this effect and whether this temporal limit can be extended through sensorimotor adaptation. Participants performed a TOJ task related to changes of the position and color of a visual stimulus in a perceptual and a motor task. A fixed change (color or position) occurred 250 or 500ms following an auditory cue (perceptual task) or the end of a manual reaching action (motor task), whereas the variable change (position or color) occurred randomly within a time window of ±200ms locked to the fixed change. The points of subjective simultaneity (PSSs) revealed that performing a voluntary action decreased the temporal asynchrony observed in the perceptual task, but only in the 250-ms delay condition. In Experiment 2, the fixed change occurred 1s after either an auditory cue or the end of a manual reaching action, and the variable change occurred either simultaneously (new sensorimotor contingencies, 60% of trials) or within a time window of ±200ms (40% of trials). The PSSs revealed that temporal asynchrony decreased in the motor task, but only after adaptation to the 1-s delay. Taken together, these data show that voluntary motor action affects the temporal binding of visual attributes for a period of less than 500ms after the end of the action. Sensorimotor adaptation can nevertheless extend this time interval, at least up to 1s.

Objective: To investigate the clinical features and neurochemical changes in Parkinson's disease with depression (PD-D).Methods: A total of 478 PD patients were divided into PD-D and PD patients without depression (PD-ND) groups according to the 24-item Hamilton Depression Rating Scale (HAMD) score. Demographic variables, motor and non-motor symptoms and activities of daily living were evaluated. The independent influencing factors of PD-D were investigated via binary logistic regression analysis. The levels of neurotransmitters in cerebrospinal fluid (CSF) were measured and their correlations with HAMD score were analyzed.Results: The proportion of PD-D was 59.0%, of which 76.95, 20.92, and 2.13% had mild, moderate, and severe depression, respectively. Anxiety/somatization was the most prevalent sub-factor of HAMD in PD-D. The scores of UPDRS III, postural instability/gait difficulty (PIGD) type and the scores of 14-item Hamilton Anxiety Scale (HAMA) and 14-item Chalder Fatigue Scale (FS) were independently associated with PD-D. The levels of dopamine (DA) and 5-hydroxytryptamine (5-HT) were all significantly reduced in PD-D group compared with those in PD-ND group. HAMD scores were negatively correlated with the DA levels in CSF.Conclusions: PD patients have a high proportion of depression, mainly of mild and moderate levels. The profile of depression in PD population is subtly different from that of the general population. Motor symptoms, PIGD type, anxiety and fatigue are the significant influencing factors of PD-D. Compared to 5-HT, DA may play a more important role in PD-D.

Application of functional data analysis to explore movements: walking, running and jumping - A systematic review

Symptomatic behaviors frequently accompany Alzheimer’s disease (AD) and include disturbances of mood, perception, and motor activity. The frequency of such symptoms reported in the literature varies depending upon symptom definition and stage of illness, but usually ranges between 20 to 40%. The biological bases that underlie these disturbances are not well understood, but probably related to the presence of specific pathological and neurochemical substrates (e.g., disproportionate pathology in limbic/paralimbic structures of the frontal-temporal lobes or changes in biogenic amine systems). A number of investigators, using different strategies and analytic methods, have shown that symptomatic behaviors are associated with a faster rate of cognitive decline. The biological significance of this association between behavior and cognition is unknown. It is conceivable, however, that the same distribution of pathologic lesions and neurochemical changes that predispose to psychosis may also hasten the rate of cognitive decline.

The effects of knee osteoarthritis on neural activity during a motor task: A scoping systematic review

This paper describes route-learning experiments with an autonomous mobile robot in which map building is achieved through a process of unsupervised clustering of sensory data. The resulting topological mapping of the robot's perceptual space is used for subsequent navigation tasks such as route following. After the autonomous mapbuilding process is completed, the acquired generalised perceptions are associated with motor actions, enabling the robot to follow routes autonomously. The navigation system has been tested extensively on a Nomad 200 mobile robot, it is reliable and copes with noise and variation inherent in the environment. One important aspect of the map building and route following system described here is that relevance or irrelevance of perceptual features is determined autonomously by the robot, not through predefinition by the designer. Secondly, the presented route learning system enables the robot to use the map for association of perception with action, rather than localisation alone.