Comparison of Microbial Ecology Between Conventional and Side-Stream Full-Scale Ebpr Systems

P11.13 Comparison of microbial ecology and antibiotic resistance before and after routine cleaning in the intensive care unit

From the west to the east across Northeast China, there are three major land use types, ranging from agricultural-pastoral interlaced land, crop land, and forest land. The soil microbial community of each land use type has been reported; however, a thorough comparison of the soil microbial ecology of soils from each land use type has not been made. In the current study, soil samples from agricultural-pastoral land, crop land, and an artificial economic forest were collected from Tongliao, Siping, and Yanji, respectively. The structure and composition of bacterial and fungal communities was investigated by a next generation sequencing protocol, and soil physicochemical properties were also determined. Pair-wise analysis showed some soil parameters were significantly different between agricultural-pastoral land and crop land or forest land, while those soil parameters shared more similarities in crop land and forest land soils. Principal coordinates analysis and dissimilarity analyses jointly indicated that bacterial and fungal communities from each sampling site were quite different. Canonical correspondence analysis and a partial Mantel test showed that the community structures of bacteria and fungi were mainly affected by clay, pH, water soluble organic carbon (WSOC), and total soluble nitrogen (TN). Co-occurrence network analysis and the associated topological features revealed that the network of the bacterial community was more complex than that of the fungal community. Clay, pH, WSOC, and NH4+-N were major drivers and pH and WSOC were major factors in shaping the network of the bacterial community and the fungal community, respectively. In brief, our results indicated that microbial diversity, co-occurrence network patterns, and their shaping factors differed greatly among soils of distinct land use types in Northeast China. Our data also provided insights into the sustainable use of soils under different land use types.

The water deer, although an internationally endangered species, is designated as a nuisance wild animal in South Korea and occupies a unique ecological niche. Studying the gut microbiome of this species is crucial for understanding its ecology. We amplified 16S rRNA DNA and compared the gut microbiomes of wild water deer from three regions with those of captive water deer from one region. Our results showed that the gut microbiome diversity of water deer did not differ significantly across regions in the wild but decreased significantly when raised in captivity. The similar microbiomes of water deer living in different regions are believed to be due to dietary diversity rather than dietary homogeneity. Furthermore, the monotony of the food supply appears to lead to significant variation in captive environments. From a conservation biology and biorestoration perspective, we suggest the importance of conserving the gut environments of animals conserved and restored outside their native habitats.

Agronomic management modifies the soil bacterial communities and may alter the carbon fractions. Here, we identify differences in several chemical and biological soil variables, as well as bacterial composition between organic (Org) and conventional (Conv) agronomic management in pecan (Carya illinoinensis) orchards located in Coahuila, Mexico. The analyzed variables were pH, N, P, K, soil organic matter, organic matter quality, soil organic carbon, C/N ratio, carbon fractions, microbial biomass carbon, easily extractable Glomalin, colony-forming units, CO2 emissions, and the enzyme activity. The DNA of soil bacteria was extracted, amplified (V3-V4 16S rRNA), and sequenced using Illumina. To compare variables between agronomic managements, t tests were used. Sequences were analyzed in QIIME (Quantitative Insights Into Microbial Ecology). A canonical correspondence analysis (CCA) was used to observe associations between the ten most abundant phyla and soil variables in both types of agronomic managements. In Org management, variables related to the capture of recalcitrant carbon compounds were significant, and there was a greater diversity of bacterial communities capable of promoting organic carbon sequestration. In Conv management, variables related to the increase in carbon mineralization, as well as the enzymatic activity related to the metabolism of labile compounds, were significant. The CCA suggested a separation between phyla associated with some variables. Agronomic management impacted soil chemical and biological parameters related to carbon dynamics, including bacterial communities associated with carbon sequestration. Further research is still necessary to understand the plasticity of some bacterial communities, as well as the soil–plant dynamics.

Microbes are the most varied category of soil creatures, although little is recognized about them. Until recently, research focused on culturable organisms; however, a lot of information is now being acquired from both culturable and, as of yet, unculturable organisms. The functions of the soil microbial community have an impact on various soil processes and, as a result, productivity. There would be no other life forms without microorganisms and their roles. A better understanding of soil microbial ecology can only improve land management decisions. Low-input agriculture systems seek to reduce the usage of synthetic fertilizers and pesticides in order to promote long-term productivity and ecosystem health. Despite the soil microbiome's critical role in agricultural output, we still have a limited grasp of microbial diversity's complex response to organic and conventional farming. The application of high input conventional farming practices, such as synthetic insecticides, herbicides, chemical fertilizers, genetically modified microorganisms, growth regulators, and other chemicals, has adversely impacted not only soil fertility but also poses serious risks to human health and the environment. Nowadays, organic farming is a viable substitute for traditional farming methods. Using sustainable agricultural practices, organic farming attempts to preserve and improve the health of the land, biodiversity, and natural resources. It plays a vital part in lowering soil erosion, nutrient leaching (especially nitrate and phosphorus), improving soil's ability to retain water, and reducing greenhouse gas emissions. One of the soil functions that agricultural land offers to civilization is the supply of habitat variety.