Compression Techniques for DNA Sequences: A Thematic Review

Glycerol-3-phosphate acyltransferases (GPATs; EC2.3.1.15) catalyze the first step in the de novo synthesis of neutral lipids (triglycerides) and glycerophospholipids. The existence of multiple enzyme isoforms with GPAT activity was predicted many years ago when GPAT activities with distinct kinetic profiles and sensitivity to inhibitors were characterized in two subcellular compartments, mitochondria and microsomes. We now know that mammals have at least four GPAT isoforms with distinct tissue distribution and function. GPAT1 is the major mitochondrial GPAT isoform and is characterized by its resistance to sulfhydryl-modifying reagents, such as N-ethylmaleimide (NEM). GPAT2 is a minor NEM-sensitive mitochondrial isoform. The activity referred to as microsomal GPAT is encoded by two closely related genes, GPAT3 and GPAT4. GPAT isoforms are important regulators of cellular triglyceride and phospholipid content, and may channel fatty acids toward particular metabolic fates. Overexpression and knock-out studies suggest that GPAT isoforms can play important roles in the development of hepatic steatosis, insulin resistance, and obesity; GPAT isoforms are also important for lactation. This review summarizes the current state of knowledge on mammalian GPAT isoforms.

INTRODUCTION: Genetic data plays a key role in the healthcare area in specific, but they are typically very large in size. Many research shows that absence of DNA information at the right time is one of the major causes of error in the healthcare area. The more genomics information that analysts secure, the better the prospects for individual and general wellbeing. Persevering and retrieving genetic information in the right form within the given time is a big challenge in the field of Healthcare. Effectively, pre-birth DNA tests screen for formative variations from the norm. Before long, patients will have their blood sequenced to detect any nonhuman DNA that may flag an irresistible illness. Later on, somebody managing malignancy will most likely track the movement of the sickness by having the DNA and RNA of single cells from various tissues sequenced every day. DNA sequencing of whole population will give a complete and better prediction of population wellbeing.OBJECTIVES: Hereditary data is growing exponentially; hence it is hard to deal with the consistently developing hereditary database. The human genome in its base configuration occupies almost thirty terabyte of storage space. Computational assets are constrained. Not just storage, transmission abilities and run time memory is likewise constrained. Data Compression is a test when the hereditary information is exponentially expanding. It is critical to save the integrity of hereditary information while packing it. Hence the main objective of this paper is to develop a lossless DNA compression algorithm that not only gives better compression but also help in retrieval of Information for efficient use in the area of Healthcare.METHODS: In this paper a lossless hereditary data compression method is being proposed. The proposed calculation works in a horizontal mode and utilization a reference based substitution technique for compression. The principle thought of this paper is in the kind of similarity scanned. All the predominant hereditary Compression methods search for similarity within the chromosome. These calculations either pursue flat mode or vertical mode for accomplishing compression. But whichever method the existing genetic compression algorithms use, they are all based on searching similarities within the chromosome i.e. they exploit only inter chromosomal similarities. The current studies focus will show that compression ratio achieved by analyzing individual chromosome is always less than the method in which we analyze and compress intra chromosomal similarities.RESULTS: This study shows that by simply using exactly matching repeats amongst all the chromosomes of the same genome, not only the compression ratio is improving but also a detailed study of all the similarities and differences between two genomes of the same species can be conducted.CONCLUSION: In this study, a new compression algorithm is being proposed for compressing DNA. Along with Inter chromosomal similarities, Intra chromosomal similarities are considered for this method. The results clearly shows that intra chromosomal matches are bigger and more than inter chromosomal matches which helps us to achieve better compression ratio.

BackgroundThe development of high-throughput sequencing technologies and, as its result, the production of huge volumes of genomic data, has accelerated biological and medical research and discovery. Study on genomic rearrangements is crucial owing to their role in chromosomal evolution, genetic disorders, and cancer.ResultsWe present Smash++, an alignment-free and memory-efficient tool to find and visualize small- and large-scale genomic rearrangements between 2 DNA sequences. This computational solution extracts information contents of the 2 sequences, exploiting a data compression technique to find rearrangements. We also present Smash++ visualizer, a tool that allows the visualization of the detected rearrangements along with their self- and relative complexity, by generating an SVG (Scalable Vector Graphics) image.ConclusionsTested on several synthetic and real DNA sequences from bacteria, fungi, Aves, and Mammalia, the proposed tool was able to accurately find genomic rearrangements. The detected regions were in accordance with previous studies, which took alignment-based approaches or performed FISH (fluorescence in situ hybridization) analysis. The maximum peak memory usage among all experiments was ∼1 GB, which makes Smash++ feasible to run on present-day standard computers.