Developing and validating a holistic welfare assessment tool for zoo-housed great apes: Integrating resource-based measures with behavioural ecology insights.

Simple SummaryEquine Quality of Life is an important concern in decision making in veterinary medicine and is especially relevant for chronically ill or aged horses when euthanasia is considered. To our knowledge, there is no assessment tool for chronically ill or aged horses that assesses equine Quality of Life defined as the horse’s evaluation of their life. However, tools exist to assess equine welfare in different contexts. Therefore, this study aimed to analyse how equine welfare, Quality of Life, well-being and happiness assessment tools define and attempt to measure these concepts. We discuss the tools’ suitability to assess equine Quality of Life in the context of end-of-life decisions for chronically ill or aged horses. Fourteen publications were found via a systematic literature search, describing ten equine welfare assessment tools and one approach to assessing equine Quality of Life in veterinary practice. Some of these welfare assessment tools have the potential to inform the development of a Quality-of-Life assessment tool supporting well-considered decision making towards the end of horses’ lives if they are adjusted to focus on the horses’ experiences, to provide an overall grade of Quality of Life and are tailored to chronically ill or geriatric horses.Equine Quality of Life (QoL) is an important concern in decision making in veterinary medicine and is especially relevant for chronically ill or geriatric horses towards the end of their lives. To our knowledge, there is no currently available QoL assessment tool for chronically ill or geriatric horses that assesses equine QoL defined as the horse’s evaluation of their life. However, tools exist to assess equine welfare in different contexts. Hence, the aims of this study were to analyse how equine welfare, QoL, well-being and happiness assessment tools label, define and operationalise the concepts and to discuss the tools’ suitability to assess equine QoL in the context of end-of-life decisions for chronically ill or geriatric horses. Fourteen articles were found through a systematic literature search, describing ten equine welfare assessment tools and one approach to integrating equine QoL in veterinary practice that suggests QoL assessment parameters. We discuss that some welfare assessment tools have the potential to support the development of a QoL assessment tool informing decisions towards the end of horses’ lives if they are adjusted to focus on the horses’ experiences, to provide an integration into an overall QoL grade and are tailored to chronically ill or geriatric horses.

Human adenoviruses (HAdVs) of species Human mastadenovirus B (HAdV-B) are genetically highly diverse and comprise several pathogenic types. AdVs closely related to members of HAdV-B infect African great apes and the evolutionary origin of HAdV-B has recently been determined in ancient gorillas. Genetic evidence for intra- and inter-species recombination has been obtained for AdVs of humans and captive great apes, but evidence from wild great apes is lacking. In this study, potential HAdV-B members of wild Eastern gorillas were analysed for evidence of recombination. One near-complete genome was amplified from primary sample material and sequenced, and from another six individuals genome fragments were obtained. In phylogenomic analysis, their penton base, pVII-pVI, hexon and fiber genes were compared with those of all publicly available HAdV-B full-genome sequences of humans and captive great apes. Evidence for intra-species recombination between different HAdV-B members of wild gorillas as well as between HAdV-B members of chimpanzees and gorillas was obtained. Since zoonotic AdVs have been reported to cause respiratory outbreaks in both humans and monkeys, and humans in West and Central Africa frequently hunt and butcher primates thereby increasing the chance of zoonotic transmission, such HAdV-B recombinants might widen the pool of potential human pathogens.

Welfare assessment in broiler farms: Transect walks versus individual scoring

Objective welfare assessments play a fundamental role in ensuring that positive welfare is achieved and maintained for animals in captivity. The Animal Welfare Assessment Grid (AWAG), a welfare assessment tool, has been validated for use with a variety of both domestic and exotic species. It combines both resource- and animal-based measures but relies heavily on knowledge of the species to effectively assess welfare. Many zoo species are understudied in the wild due to their cryptic nature or habitat choice; therefore, the published literature needs to be supported with captive behavioural observations and zoo records. Here we adapted previously published AWAG templates to assess the welfare of Crossarchus obscurus. A total of 21 factors were identified, and the final template was used to retrospectively score the welfare of two male and two female C. obscurus at Marwell Zoo, UK, validating the use of this process for preparing a welfare assessment for a species where the published literature is scarce.

In the wake of the chimpanzee genome publication, Pascal Gagneux, James J. Moore and Ajit Varki consider the ethical and scientific challenges for scientists who work on captive great apes. The cover photo by Kevin Langergraber shows the adult female chimpanzee ‘Jolie’ in Kibale National Park, Uganda. This was taken on 16 August 2004, a few weeks before Jolie gave birth to her first infant. This week marks a landmark in the study of our closest living relative: the publication by the Chimpanzee Sequencing and Analysis Consortium of the initial sequence of the chimpanzee genome, together with a comparison with the human genome. The paper describes changes that have shaped human and chimpanzee species since the split from our common ancestor, and hints at what makes us uniquely human: 35 million single-nucleotide substitutions, 5 million small insertions and deletions, local rearrangements and a chromosome fusion. A comparison of gene duplications in chimpanzee and human genomes reveals gene expression differences that may underlie disease susceptibility. A study of primate genomes shows that subtelomeres are hot spots of recent chromosomal duplication and gene conversion. Conservation of Y-linked genes during human evolution is revealed by comparative sequencing in the chimpanzee. The final research paper in this collection fills a big gap in our knowledge: the first chimpanzee fossils ever found show that chimps and early humans inhabited the same environments in which they evolved and diverged. The fossils — three teeth — are from half-million-year-old sediments in Kenya that also yielded fossils of Homo. Four Progress reviews accompany these papers, looking at chimp culture, social behaviour, psychology and cognition. Elsewhere in the issue, researchers talk about working with chimpanzees, a feature summarizes other primate genome projects, and in two Commentaries, important ethical issues surrounding research on great apes are considered.

Cardiovascular disease (CVD) is a significant cause of morbidity and mortality in great apes that are managed in zoological institutions. The impact of CVD on captive great ape populations is concerning and detection can be difficult because of the lack of definitive clinical signs prior to death. The purpose of this paper is to provide an overview of the efforts of the Great Ape Heart Project (GAHP) and current understanding of CVD in captive great apes, and outline the key considerations for assessing and treating CVD in these populations.

Good zoos have four aims-to conserve species, educate the public, engage in research, and provide recreation-all of which can only be achieved when underpinned by high animal welfare standards. In this paper, we share the approach that The Royal Zoological Society of Scotland's (RZSS) Edinburgh Zoo and Highland Wildlife Park take to animal welfare. We highlight the role that animal welfare research, in collaboration with universities, has had in enabling the zoo to take an evidence-based approach to welfare and to put findings into practice. We share the collaborative process through which we developed and piloted the current animal welfare assessment tools, how they were validated, and how they were tested for reliability as part of a long-term collaboration between the Royal Zoological Society of Scotland and the University of Stirling: (1) the RZSS Welfare Assessment Tool, a 50-question animal welfare assessment adapted from the British and Irish Association of Zoos and Aquariums (BIAZA) Toolkit; and (2) the Stirling Toolkit, a package of evidence-based resources for behavioural-data collection. Our aim is to facilitate standardised, evidence-based approaches to assessing animal welfare which, when finalised, can be used collaboratively across zoos.

Development of a welfare assessment protocol for practical application in Argentine feedlots

Simple SummaryZoological collections are constantly working to find new ways of improving their standards of care for the animals they keep. Many species kept in zoos are also found in private animal collections. The knowledge gained from studying these zoo-housed populations can also benefit private animal keepers and their animals. Waterfowl (ducks, geese, and swans) are examples of species commonly kept in zoos, and in private establishments, that have received little attention when it comes to understanding their core needs in captivity. Measuring welfare (how the animal is coping with the environment that it is in) is one way of understanding whether a bird’s needs are being met by the care provided to it. This paper provides a method for measuring the welfare of waterfowl that can be applied to zoo-housed birds as well as to those in private facilities. The output of such a welfare measurement method can be used to show where animal care is good (and should be continued at a high standard) and to identify areas where animal care is not meeting the bird’s needs and hence should be altered or enhanced to be more suitable for the birds being kept.Welfare assessment is a tool to both identify welfare challenges and to evidence where current husbandry practices support positive welfare outcomes. Such tools are becoming more available and can be amended based on the nature of the facility and needs of taxonomic groups. Currently, welfare assessment has a strong mammalian theme, and some behavioural measures of welfare commonly applied to mammals do not translate well for other taxa. This paper provides a method for welfare assessment of Anseriformes; widely housed, diverse bird species kept under a range of management styles. A mixture of resource-based (i.e., determination of aspects of the physical environment or the bird’s physical appearance or activity) and animal-based (i.e., observations that equate to a bird’s feelings or personality characteristics) measures are integrated to enable a full review of potential predictors of welfare. The method provides a rapid and valid way for all personnel to collect information that evaluates quality-of-life experiences of the Anseriformes under their care. Explanations of key terminology are provided to enable repeatable and reliable assessment for all persons using the tool. Suggestions for follow-up actions are provided to emphasise why the welfare assessment process needs to be one of continual re-evaluation of animal care.

Respiratory infections are a leading cause of death in developing countries. Infants and young children are especially susceptible to disease because they lack immunity, whereas adults who have acquired immunity can be infected asymptomatically. Great ape species, all of which are endangered, are similarly susceptible to respiratory illnesses caused by human respiratory pathogens. We obtained 432 nasopharyngeal swab samples (127 from adults and 305 from children) in a cross-sectional study that took place between February and October 2022 at four sites in Western Uganda (Budongo Central Forest Reserve, Bulindi Town Council, Bwindi Impenetrable National Park, and Kibale National Park) where the participants live in communities where interaction with apes is frequent. Prior research at Kibale has shown that locally circulating human respiratory pathogens have led to multiple lethal outbreaks in wild eastern chimpanzees (Pan troglodytes schweinfurthii). We used a multiplex PCR panel to characterize respiratory pathogens, with the goal of assessing whether respiratory illnesses in the chimpanzees of Budongo and Bulindi and the mountain gorillas (Gorilla beringei beringei) of Bwindi might have originated in local children and been introduced to the apes via asymptomatic adult carriers. The prevalence of respiratory pathogens was twice as high in Bwindi (44.0%) as it was in Budongo (24.0%) and Bulindi (20.8%), while the prevalence was intermediate at Kibale (34.4%). Rates of respiratory pathogen detection were higher but statistically indistinguishable in children compared to adults at Budongo and Bulindi, and children were 15.0 times more likely than adults to have positive detections at Kibale. At Bwindi, however, the pattern was reversed, with adults 2.6 times more likely than children to have positive detections. Rhinovirus, metapneumovirus, human parainfluenza virus 3, respiratory syncytial virus, and coronavirus OC43, all of which have been identified as causative agents of respiratory disease outbreaks in great ape populations across sub-Saharan Africa, accounted for three quarters (73.6%) of detected pathogens. Our data support the idea that human respiratory pathogens that can infect great apes occur at high frequencies in human populations in Western Uganda that live close to and interact with wild apes that have suffered from lethal outbreaks caused by these same pathogens. Reducing respiratory infections in local children, thereby reducing both carriage of those infections into the forest by people and ape exposure to these pathogens when they enter human spaces, should decrease the risk of respiratory disease outbreaks in apes.

There has been a growing interest in developing and implementing animal welfare assessment schemes on farms, zoos and aquaria, experimental situations, and even on free-ranging animal populations. Inspection bodies and organizations that carry out farm inspection for retailers and for government have started to use animal welfare outcome-based measures (OBMs), like behaviour or physical condition, as a progression from resource-based measures (RBMs), such as the number of feeders or the size of the living accommodation, in their inspection schemes. Measures based directly on the animals can provide welfare indicators under a variety of conditions, as welfare is a characteristic of the individual animal, not just of the system in which animals are kept. Many modern farms, zoos, aquaria, laboratories and other facilities are keen to identify welfare-monitoring tools that can be applied readily, allow for rapid assessment and provide timely feedback for management decisions. Improvements in welfare can be achieved through the combination of: (i) measurement; (ii) analysis of risk and environmental factors; (iii) provision of information resulting from the assessment; and (iv) promotion of positive change by supporting management decisions. In principle, individual measures can also be combined to give aggregate scores that can be presented to the animal keeper or the consumer. This requires the attribution of weighted values to the measures, to assess the impact of each measure with respect to animal welfare. Four questions arise about any approach assessing the animals themselves: is it practical, is it valid (providing 'real' information about welfare), is it repeatable and is it robust (not influenced by weather, etc.)? Yet animal experience cannot be reduced to a mechanistic assessment: animals are variable, living and sentient beings and this must be addressed realistically in practical assessment systems. A multi-pronged strategy involving various RBMs and OBMs is most likely to provide the capacity for comprehensive welfare monitoring. Such a strategy may include regularly updated species care guidelines, accreditation standards, longitudinal, multidisciplinary and multi-institutional studies, and assessment tools for continuous welfare monitoring. Some case studies are discussed in detail: welfare assessments of broiler chickens on farms, and clouded leopards, elephants and dolphins in zoos. An application is then described related to scoresheets for refinement measures in laboratory animal research.

Welfare assessments have been largely successful in improving management and quality of life for animals in human care. This has prompted an increased interest in their use for free-ranging wild animals to assess health, environment, and human-induced impacts that influence policy decisions. The North Atlantic Right Whale (NARW, Eubalaena glacialis) is one of the most endangered whale species. NARWs constantly face serious injuries and mortalities due to human activities, which poses both a species conservation and an individual welfare concern. Establishing a standardized welfare assessment for the NARW is a holistic way to understand the cumulative effects of anthropogenic activities at both the individual and population levels. To investigate the potential use of welfare assessments in NARWs, we performed a brief literature review to explore the history and utility of animal welfare assessments. Following the review, we developed a welfare assessment tool specific to the NARW. The goal is for biologists to apply this tool to understand NARW welfare in conjunction with research in the field. Ultimately, the information gained from this review can aid in public dissemination of the results of human impacts on NARW welfare and may help influence future conservation policies.

Measurement of zoo animal welfare states enables improvement to husbandry and management to be evidence-based and implemented according to species’ needs. Theoretical welfare concepts are often discussed, and whilst it is helpful to ensure wide consensus across all stakeholders on what welfare comprises, practical application of such evidence-based information is as equally important. All species housed in zoos will have specific needs that must be met by their housing, husbandry, and care to enable them to thrive. Therefore, this paper examined how to identify key animal care needs and an animal’s responses to them to form a basis for species-specific welfare assessment approaches. There are examples of familiar-to-the-zoo species that still pose challenges regarding delivery of optimal husbandry and management. As such, the identification and evaluation of core concepts of the biology, behaviour, “needs and wants” of these species is required to support validation and refinement of physical, behavioural, and psychological welfare measures. This article evaluated the use of evidence to build capacity in welfare measurement for a familiar zoo-housed species, the giraffe (Giraffa camelopardalis, Linnaeus 1758), by outlining seven key needs and requirements that must be provided in the zoo (“giraffe W-E-L-F-A-R-E” = Warmth, Enrichment, Leaves, Feeding, Alfalfa, Rumination, Exercise). Provision of these inputs, and opportunities for all giraffes in a herd to engage with them, provides the foundation for further welfare assessment to be implemented. Specifically, the validation and measurement of mental states that are more likely to be positive if key behavioural and ecological needs have already been met. This paper advocated for this evidence-based approach to “welfare-focussed husbandry”, with distillation of key information that supports species-relevant care, to be developed for other zoo-housed species as support for their welfare assessment protocols. Such welfare-focussed husbandry is layered on top of the basic animal care requirements of the species in the zoo to ensure all individuals have the best opportunity to attain positive welfare states. In this way, and once validated, foundational welfare assessment can be easily completed by busy animal care staff, capacity is built into zoo operations as all stakeholders are aware of exactly what each species needs, and deeper dive welfare assessment (especially concerning animal mental states) can be targeted more effectively. Further evolution of these seven steps for the giraffe is suggested and extrapolation of this approach, to aid identification of key welfare indicators across all zoo-housed species, is encouraged.

Animal welfare is increasingly important for the Australian livestock industries, to maintain social licence to practice as well as ensuring market share overseas. Improvement of animal welfare in the livestock industries requires several important key steps. Paramount among these, objective measures are needed for welfare assessment that will enable comparison and contrast of welfare implications of husbandry procedures or housing options. Such measures need to be versatile (can be applied under a wide range of on- and off-farm situations), relevant (reveal aspects of the animal’s affective or physiological state that is relevant to their welfare), reliable (can be repeated with confidence in the results), relatively economic to apply, and they need to have broad acceptance by all stakeholders. Qualitative Behavioural Assessment (QBA) is an integrated measure that characterises behaviour as a dynamic, expressive body language. QBA is a versatile tool requiring little specialist equipment suiting application to in situ assessments that enables comparative, hypothesis-driven evaluation of various industry-relevant practices. QBA is being increasingly used as part of animal welfare assessments in Europe, and although most other welfare assessment methods record ‘problems’ (e.g. lameness, injury scores, and so on), QBA can capture positive aspects of animal welfare (e.g. positively engaged with their environment, playfulness). In this viewpoint, we review the outcomes of recent QBA studies and discuss the potential application of QBA, in combination with other methods, as a welfare assessment tool for the Australian livestock industries.

Wild penguins are facing increased threats to their populations and their welfare as a consequence of human activities. Understanding the perception of animal welfare is essential to identify ethical concerns related to the negative impact of anthropogenic factors on wild species and to guide conservation efforts that reflect societal values. Since penguin conservation is of general interest, we examined the human dimension of welfare assessment across a range of interest groups concerned with penguins, seabird biology and wildlife conservation. We provided participants with a Penguin Welfare Assessment Tool (PWAT) based on the five domains model. The PWAT supports consideration of the impact of four physical aspects on welfare-relevant mental states. Bibliometric analysis of keywords from 347 scientific articles indicated that penguins around the world face five main types (themes) of anthropogenic factors and we then developed five hypothetical scenarios, each related to one theme. Seventy-five participants scored the overall impact of the events described in the scenarios on penguin welfare as negative using the PWAT. Participants rated short-duration, high-intensity events (i.e., being trapped in a ghost fishing net) as having a significantly more severe impact on penguin welfare than low-intensity, long-duration events (P < 0.0001). Scores provided by participants for each domain for each scenario were largely as expected and we found good correlation (all P < 0.0001) between the physical domains and “mental state” for all scenarios, indicating that the tool was facilitating the participants' assessment of welfare. No evidence was found that experience of working or studying penguins, or indeed any other demographic factor investigated, influenced the assessments of welfare. We found little agreement between participants in the scores provided (unalike scores mostly between 0.7 and 0.8), and agreement between participants with experience of working with penguins was no better than between participants without such experience. We discuss the possibility that low agreement within different interest groups may be improved by providing more scientific information to support the evaluation of penguin welfare. We conclude that scientific knowledge of penguin biological responses to anthropogenic factors is vital to support the evaluation of wild penguin welfare by the public and other stakeholders.