Enhanced solubility of chicken liver insoluble protein by pH-shifting modification: physicochemical and structural properties.

With increasing consumer demand for healthy and sustainable food, plant proteins have been used widely in meat substitutes, dairy alternatives, and functional foods. However, in comparison with animal proteins, plant proteins often exhibit weaker functional properties, such as solubility, emulsifying, and gelation, which limit their application in food processing. The aim of this study was to investigate the effects of high-intensity ultrasound treatments (HIUTs) on the physicochemical properties, structural characteristics, emulsifying properties, and antioxidant capacity of shiitake mushroom protein isolate (SMPI). This study investigates how HIUTs affect SMPI. It also provides insights into potential applications within the food industry. When treated with a 300 W intensity ultrasound, SMPI exhibited higher solubility, water-holding capacity (WHC), oil-holding capacity (OHC), and antioxidant activity in comparison with the control (0 W). These results indicate that HIUT can enhance the functional properties of SMPI significantly. The molecular structure of SMPI after ultrasound treatment also underwent significant changes, suggesting that the treatment affects not only the physical properties but also the spatial conformation of SMPI. The concentration of free sulfhydryl (-FSH) group concentrations increased from 3.615 ± 0.080 μmol g-1to 13.020 ± 0.061 μmol g-1, and the emulsifying ability index (EAI) significant increased, indicating that a significant change produced in the tertiary structure of SMPI. The findings suggest that ultrasonic treatment can alter the physicochemical and functional attributes of SMPI significantly, increasing its potential for use within the food industry. © 2024 Society of Chemical Industry.

Towards sustainable production of edible cricket protein concentrates: Impact of different defatting pre-treatments and extraction methods on the structural, physicochemical, and techno-functional properties of Acheta domesticus L. proteins.

Characterization of the physicochemical, thermal and rheological properties of cashew kernel starch

Response surface methodology was used to optimize the processing conditions in the preparation of cowpea tempeh. The independent factors studied were boiling time (varying from 5 to 30 min), incubation time (varying from 12 to 48 h) and incubation temperatures (varying from 25 to 50C), whereas the dependent factors were protein content, protein solubility, pH, titratable acidity and total color difference (using L, a* and b*). Regression models were generated and adequacy was tested with regression coefficients (R2) and the lack‐of‐fit tests. Optimum processing conditions were determined by method of superimposition. There was a strong and significant influence (P < 0.01) of the quadratic effect of the incubation time on the protein content of the cowpea tempeh, with similar significance (P < 0.01) noted in protein solubility with increasing boiling time. The optimum processing conditions observed for the preparation of cowpea tempeh were boiling time of about 20 min, incubation time of about 28 h and incubation temperature of about 37C.PRACTICAL APPLICATIONSResponse surface methodology (RSM), as a statistical tool, has been effectively used in food process applications. This study embraced the use of RSM in the optimization of the processing conditions involved in the preparation of cowpea tempeh. Superimposition of the contour plots developed from the regression models indicated that cowpea with optimum quality characteristics should be processed at a boiling time of 20 min, incubation time of 28 h and incubation temperature of 37C. These conditions could be adopted for the industrial production of cowpea tempeh.

Sixteen commercially wet‐extracted protein isolates and 13 commercially air‐classified protein concentrates were evaluated for their particle size, physicochemical properties (protein solubility, differential scanning calorimetry, surface hydrophobicity, and surface charge) and functional properties (water holding capacity, oil absorption capacity, emulsifying capacity and stability, foaming capacity and stability, and least gelling concentration). Protein concentrates exhibited lower average values for particle size, surface charge, surface hydrophobicity, and water holding capacity, but higher values for oil absorption capacity, protein solubility, emulsion capacity and stability, as well as foaming capacity and stability, compared to protein isolates. Intensive processing appeared to denature proteins in most isolates, leading to reduced functional properties. Changes in pH and the addition of 2% NaCl influenced the properties of both protein isolates and concentrates. Protein solubility, emulsion capacity and stability, and foaming stability of the protein samples were superior at pH 10, followed by pH 3. The addition of 2% NaCl enhanced foaming capacity and reduced the least gelling concentration. A significant interaction between sample and treatment conditions ( p < 0.001) in protein isolates and protein concentrates was observed. Crop type differences in protein solubility, oil emulsion capacity and foaming stability of protein concentrates under certain conditions were detected using maximum likelihood factor analysis (MFLA). However, these differences were not evident in protein isolates, possibly due to more variable processing conditions.

Static magnetic field-assisted fermentation of corn dietary fiber by Hericium erinaceus: Insight into fermentation mechanisms and related characteristics.

Pasting, gelatinization, and retrogradation characteristics related to structural properties of tea seed starches

The physicochemical and functional properties of convection oven- and freeze-dried gluten meals of two corn varieties were evaluated. The physicochemical properties (water solubility index, water absorption index, Hunter color parameters, and bulk density) and functional properties (water absorption, oil absorption, least gelation concentration, protein solubility, and emulsification properties) of convection-oven and freeze-dried corn gluten meals were compared with each other and soy flour. Freeze-dried corn gluten meals was observed to have lower bulk density (0.244–0.263 kg/m3) and was lighter in color (high L and ΔE) compared to their counterpart convection oven–dried gluten meals. Freeze-dried gluten meals from both corn varieties showed significantly higher oil absorption, water absorption, pH, emulsification, and protein solubility compared to oven-dried corn gluten meals. The gluten meals from both corn varieties had lower water absorption and bulk density but higher oil absorption than soy flour, suggesting the hydrophobic nature of corn proteins. Corn gluten meals formed thin (pourable) emulsions compared to soy flour emulsions, which were thick salad dressing type. Freeze- and convection oven–dried corn gluten meals showed significantly lower protein solubility measured at different pH than soy flour.

The effects of polyphenol removal from common buckwheat seed flours with cold aqueous organic solvents (including 95% ethanol, 70% 2-propanol, and 80% methanol, v/v) on the physicochemical and conformational properties of their protein isolates (BPI) were investigated. The extraction resulted in considerable reduction in its polyphenol content, especially protein-bound polyphenol content, and concomitant increase in its protein content. The efficiency of the removal of the polyphenols was much better in the 2-propanol case than in other two cases. The surface hydrophobicity of the proteins changed slightly, while the disulfide bond contents remarkably increased, partially at the expense of free sulfhydryl group contents. The protein solubility in the pH range of 7.0-11.0 and the proportion of undenatured globulins in BPI products were variably improved by the organic solvent extraction, and the extent of the improvements was highest in the 2-propanol case. Intrinsic emission fluorescence and far-UV and/or near-UV CD spectra showed that polyphenol removal resulted in significant changes in tertiary and/or secondary conformations of the proteins in BPI, and the changes were also related to the efficiency of the removal of the polyphenols. These results suggest that the physicochemical and conformational properties of BPI are closely related to its polyphenol level, and there is also a close relationship between its physicochemical properties and tertiary and/or secondary conformations.

Yeast protein as a novel dietary protein source: Comparison with four common plant proteins in physicochemical properties

Optimizing aquasoya protein: Insights of various pre-treatments for enhancing the upcycling potential from small black bean by-product

Vegetable milks containing antioxidants, fatty acids and vitamins can be recommended as an alternative to animal milks cause some health problems like lactose intolerance and milk protein allergy. Hazelnut oil cake which is a waste from hazelnut oil production cannot be used for human nutrition. Cold press hazelnut cake must be evaluation as food materials. The aim of this study was to evaluate the effect of cold pressed hazelnut cake concentration (5, 10, 15% w/v) and high pressure homogenization (HPH) (up to 100 MPa) on the physicochemical, structural and sensory properties of hazelnut milk and also determined the best conditions for hazelnut milk production by TOPSIS approach. Hazelnut milks produced from 15% hazelnut cake had the best physicochemical properties and physical stability, but the sensory properties of the milks were not acceptable due to viscoelastic behaviors. Physicochemical properties, physical stability and sensory characteristics of hazelnut milks were significantly affected by homogenization pressure (P < 0.05). Colloidal stability and sensory properties of hazelnut milks were improved by increasing homogenization pressure. The viscosity values of hazelnut milks with 10 and 15% hazelnut cakes were significantly decreased by increasing the homogenization pressure. TOPSIS approach showed that 10% hazelnut cake concentration and 100 MPa homogenization pressure was the best condition for an acceptable hazelnut milk production.

Sheep placenta is a functional food with proven pharmacological benefits, but its quality is often compromised by conventional drying methods. This study aimed to identify the optimal drying method for preserving the quality of sheep placenta by evaluating the effects of hot air drying (HAD), baking (BK), and vacuum freeze drying (VFD) on its appearance, physicochemical properties, and odor. Physicochemical properties were assessed using colorimetry, texture profile analysis, low-field nuclear magnetic resonance, and amino acid profiling. Volatile compound analysis was performed by headspace gas chromatography-mass spectrometry coupled with an ultrafast gas chromatography electronic nose. Results showed that vacuum freeze dried samples maintained higher lightness, more intact microstructure, better retention of essential amino acids, and significantly lower fishy odor compared to samples treated with hot air drying or baking. Multimethod analysis (Mantel test, Pearson correlation, and TOPSIS) consistently identified vacuum freeze drying as the most effective process. These findings demonstrate that vacuum freeze drying is scientifically preferable for minimizing quality degradation in sheep placenta, providing valuable insights for its application in functional food processing.

Impacts of pyrolysis temperatures on physicochemical and structural properties of green waste derived biochars for adsorption of potentially toxic elements

Effects of salting-in/out-assisted extractions on structural, physicochemical and functional properties of Tenebrio molitor larvae protein isolates