Evaluation of detection methods for Legionella species using seeded water samples

Various industries throughout the world require Standard methods for trade of products and referee analysis. Over the past 20-30 years, a number of wet-chemical Standard methods have been developed and are in use. Due to the large number of elements required for analysis in most products, XRF methods have become attractive as techniques to handle many elements simultaneously with a simple single sample preparation. Despite XRF being in daily use in many industries as an {open_quote}in-house{close_quote} method, there are relatively few National or International Standard XRF methods. For some years, Norrish and his associates (CSIRO Division of Soils, Adelaide, Australia) have been involved in the preparation of Australian and International Standard XRF methods based on borate fusions. A method for the analysis of nine elements in iron ores has recently been published by the International Standards Organization (ISO 9516, 1992). This method has been expanded to include iron and trace elements, some twenty elements in all, and will be tested on an international scale in 1995. A method document for the analysis of twenty elements in titaniferous sands has been drafted and submitted to Standards Australia for public review (DR 93129, 1993) prior to publication as an Australian Standard method.more » In addition, a test program has been completed for the analysis of fourteen elements in zircon sands and the method document is currently being drafted. This paper will outline the general approach and techniques of the Standard methods, using the methods developed for the Australian mineral sands industry for the analysis of titaniferous mineral sands (ilmenite and rutile) and for zircon sands as examples. 3 refs., 9 tabs.« less

Water samples collected during routine biomonitoring of the upper Ganges river were analysed for coliform counts by the multiple tube (MPN) and membrane filter (MF) techniques. The data obtained were subject to statistical analysis. Consistently greater coliform counts were recorded using the MPN technique, although all the MF values produced fell within 95% of those obtained by the MPN method, with a linear co‐relation of +0.982. The effect of turbidity on the lower recovery by MF technique was observed.

Because Clostridium perfringens spores are both specific to sewage contamination and environmentally stable, they are considered as possible conservative indicators of human fecal contamination and possible surrogates for environmentally stable pathogens. This review discusses the reasons and summarizes methods for monitoring spores in water. Cultural methods are still preferred over qPCR for routine water quality monitoring because of their low costs. Membrane filter (MF) methods are preferred over the more laborious and less accurate most probable number methods. The most commonly used MF media are TSC medium and mCP medium. TSC normally allows higher recoveries than mCP. TSC produces fewer false-positive results than mCP; however, it does produce more false-negatives. Two newer methods have substantial potential, CP Chromo Select agar, which allows better recoveries and greater specificity than mCP, and the Fung double tube method, which creates anaerobic conditions and allows enumeration of colonies in tubes in 5-6 hours. Aerobic spores are not associated with fecal contamination but they can be surrogates for environmentally stable pathogens in monitoring water for treatment efficacy; Bacillus cereus spores are normally measured on nutrient agar by the MF method.

Monitoring of waterborne pathogens is improved by using concentration methods prior to detection; however, direct microbial enumeration is desired to study microbial ecology and human health risks. The aim of this work was to determine Salmonella presence in river water with an ultrafiltration system coupled with the ISO 6579:1993 isolation standard method (UFS-ISO). Most probable number (MPN) method was used directly in water samples to estimate Salmonella populations. Additionally, the effect between Salmonella determination and water turbidity was evaluated. Ten liters or three tenfold dilutions (1, 0.1, and 0.01 mL) of water were processed for Salmonella detection and estimation by the UFS-ISO and MPN methods, respectively. A total of 84 water samples were tested, and Salmonella was confirmed in 64/84 (76%) and 38/84 (44%) when UFS-ISO and MPN were used, respectively. Salmonella populations were less than 5 × 10(3) MPN/L in 73/84 of samples evaluated (87%), and only three (3.5%) showed contamination with numbers greater than 4.5 × 10(4) MPN/L. Water turbidity did not affect Salmonella determination regardless of the performed method. These findings suggest that Salmonella abundance in Sinaloa rivers is not a health risk for human infections in spite of its persistence. Thus, choosing the appropriate strategy to study Salmonella in river water samples is necessary to clarify its behavior and transport in the environment.

Microbial contamination and extensive growth of bacteria in the drilling mud impact drilling operation and reservoir integrity. To reduce the damaging effect of microbial growth, drilling mud is generally treated with biocides for microbial control and preventing loss of rheological properties from biodegradation. Make-up water and drilling mud samples from reserve tank and mixing tank were collected from three drilling rigs in Saudi Aramco oilfields. The baseline number of general aerobic bacteria (GAB) and sulfate-reducing bacteria (SRB) in make-up water and drilling mud samples were determined using the Most Probable Number (MPN) method. Microbial growth in the drilling mud and microbial control using glutaraldehyde-based biocide were evaluated under the conditions simulating short-term (<24 hours) and long-term (up to 7 days) downtime of drilling operations. In short operational downtime, the current practice in Saudi Aramco with no biocide addition resulted in high number of GAB (105-107/ml) in the mud samples. In long operational downtime, addition of 1500 ppm of biocide (1/3 of normal dosage) at Day 3 was effective in controlling GAB and SRB growth in drilling mud. Biocide performance was significantly affected by sulfide content in the mud; higher dosage and more frequent treatment may be required to control the microbial growth in the drilling mud. The study indicated that biocide treatment is essential for microbial control in drilling operation, especially in the period of operational downtime. Finally, drilling mud operation and field best practice for microbial control were recommended to be implemented in all Saudi Aramco drilling rigs.

Binding of prolactin and monoclonal antibody to prolactin receptors immobilized on a nitrocellulose membrane filter

Assessment of physicochemical and antibacterial properties of structured water samples from Ota, Ogun State, Nigeria

Legionella Pneumonia and AIDS

Legionella Gratiana sp Nov. isolated from French SPA water

Journal of the Society of Chemical IndustryVolume 32, Issue 22 p. 1039-1041 London Section Analysis of crude glycerin by the international standard methods, 1911 R. G. Grimwood, R. G. GrimwoodSearch for more papers by this author R. G. Grimwood, R. G. GrimwoodSearch for more papers by this author First published: 29 November 1913 https://doi.org/10.1002/jctb.5000322201Citations: 1AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinked InRedditWechat Citing Literature Volume32, Issue2229 November 1913Pages 1039-1041 RelatedInformation

Although direct fluorescent-antibody (DFA) testing has been used successfully for a number of years to detect legionellae in clinical specimens, the number of known species and serogroups of Legionella has now increased to such an extent that the performance of DFA testing for all serological variants is impractical. Lung homogenates that were submitted to the Centers for Disease Control, Atlanta, Ga., from patients with suspected legionellosis, from November 1977 through May 1982, were originally screened by DFA testing. In our study 498 of these lung homogenates were screened by indirect fluorescent-antibody (IFA) testing, using a panvalent antiserum pool containing antibodies to 25 serological variants of Legionella spp. Fluorescein isothiocyanate-labeled goat anti-rabbit immunoglobulin was used as the second antibody of the sandwich system. For positive homogenates, i.e., those containing Legionella organisms, species and serogroup identification was made by IFA staining with polyvalent serum pools and then with monovalent antiserum. Of the 498 homogenates screened, 39 (7.8%) were positive by IFA testing. Four (0.8% of total; 10.3% of positive homogenates) of these had previously been negative by DFA testing, but subsequent testing showed that they contained Legionella organisms for which DFA reagents were not available at the initial screening. All specimens that were positive by DFA testing were also positive by IFA testing. IFA testing with polyvalent antisera is a simple, efficient method which is at least as sensitive as DFA testing and which can be used by clinical laboratories to cope with the increasing number of known serological variants of Legionella spp.

Relatam os autores isolamento de Legionella pneumophila sorogrupo 1, acompanhado de evidências sorológicas de infecção atual, em homem de 40 anos com infecção respiratória grave que evoluiu para insuficiência respiratória aguda. Esta foi caracterizada por hipoxemia severa refratária a altas concentrações de oxigênio e radiograficamente por infiltrados difusos em ambos pulmões. Com introdução de clindamicina, amicacina, ceftriaxone e ventilação à volume com Pressão Expiratória Positiva Final (PEEP) de 14 cm de H(2)0, houve estabilização do quadro e gradual recuperação. Suspeitando-se de legionelose, foi colhido sangue e secreção traqueal para exames específicos. A secreção traqueal foi semeada em meio BCYE com isolamento de bacilo gram-negativo, identificado como Legionella pneumophila sorogrupo 1 por características culturais, bioquímicas e reações de imunofluorescência direta e de aglutinação em lâmina. O estudo sorológico revelou títulos de anticorpos 128, 1024, 4096 e 8192 para amostras coletadas na 1ª, 3ª, 4ª e 6ª semanas após o início do quadro. Os resultados definitivos foram obtidos com o paciente em recuperação. É realçada a comprovação da presença de Legionella sp. como agente patológico em nosso meio; a importância das medidas de suporte na evolução do paciente; a necessidade de se pensar neste agente no diagnóstico diferencial de pneumonias e de se pesquisar mais esta etiologia com metodologia laboratorial específica.

Impact of Improved Sanitation Standard Operating Procedures on Microbial Populations at Three Tree Fruit Packing Facilities.

An annual cycle of abundance and activity was determined for bacteria in Newfoundland coastal water that is dominated by the Labrador Current. Both in abundance and activity, the population showed characteristics similar to those reported for bacteria in cold ocean environments. The number of bacteria per litre determined by the acridine orange direct count method ranged from an average low of 0.25 × 108 in winter to an average high of 2.8 × 108 in summer. Activity, determined by the kinetic method, was correlated with temperature. The average heterotrophic potential (Vmax) was 3 ng glutamate. L−1∙h−1 in winter and 54.7 ng glutamate. L−1∙h−1 in summer. The average turnover times were 2632 and 256 h, respectively, during the same periods. The population of hydrocarbonoclastic bacteria, estimated by a most probable number method, constituted 4.4% of the viable bacterial population estimated by the standard plate count method, although the former were most abundant near the water's surface. The number of hydrocarbonoclastic bacteria was correlated with temperature and with Vmax of the heterotrophic bacterial population.

Polyphosphate provides a substitute for ATP and energy source when phosphorus is a limiting resource in nature. The present study focuses on the role ofpolyphosphate for the survival of Vibrio cholerae in the aquatic habitats as an autochthonous bacterium. The survival advantages of polyphosphate of V. cholerae O1 having (wild type) and lacking (mutant) polyphosphate kinase (ppk) gene in surface water and with Anabaena variabilis were compared by cultural, Direct Fluorescent Antibody (DFA) and polymerase chain reaction methods in natural water microcosms. The microcosm's water was prepared by filtering and physicochemical parameters were also investigated by standard methods. The results revealed that both fresh and saline water, the wild type strain enhanced survival in cultural conditioned than ppk mutant strain. However, Fluorescent Antibody Direct Viable Counts (FADVC) and Polymerase Chain Reaction (PCR) results noted both strains have the equal survival strategy in viable but nonculturable state (VNC). In conclusion, it could be hypothesized that the polyphosphate inclusion body might keep cultivable and survivable at low phosphate natural environment of the aquatic bacterium.