Fabrication of Chemofluidic Integrated Circuits by Multi-Material Printing

Multiscale and multimaterial three-dimensional (3D) printing is new frontier in additive manufacturing (AM). It has shown great potential to implement the simultaneous and full control for fabricated object including external geometry, internal architecture, functional surface, material composition and ratio as well as gradient distribution, feature size ranging from nano-, micro-, to macro-scale, embedded components and electrocircuit, etc. Furthermore, it has the ability to construct the heterogeneous and hierarchical structured object with tailored properties and multiple functionalities which cannot be achieved through the existing technologies. That paves the way and may result in great breakthrough in various applications, e.g., functional tissue and organ, functionally graded (FG) material/structure, wearable devices, soft robot, functionally embedded electronics, metamaterial, multifunctionality product, etc. However, very few of the established AM processes have now the capability to implement the multimaterial and multiscale 3D printing. This paper presented a single nozzle-based multiscale and multimaterial 3D printing process by integrating the electrohydrodynamic jet printing and the active mixing multimaterial nozzle. The proposed AM technology has the capability to create multifunctional heterogeneously structured objects with control of the macroscale external geometry and microscale internal structures as well as functional surface features, particularly, the potential to dynamically mix, grade, and vary the ratios of different materials. An active mixing nozzle, as a core functional component of the 3D printer, is systematically investigated by combining with the theoretical analysis, numerical simulation, and experimental verification. The study aims at exploring a feasible solution to implement the multiscale and multimaterial 3D printing at low cost.

In the Fused Filament Fabrication (FFF/FDM) technology, the multi-material manufacturing additive method is achieved by a single nozzle or multiple nozzles working simultaneously with different materials. However, the adhesion between different materials at the boundary interface in FDM multi-material printing is a limiting factor. These studies are concerned with improving and study the adhesion between two polymers.Due to the numerous applications and possibilities of 3D printed objects, combining different materials has become a subject of interest. PLA is an alternative to the use of petrochemical-based polymers. Thermoplastic Polyurethane is a flexible material that can achieve different characteristics when combined with a rigid filament, such as PLA. To improve the adhesion between PLA and TPU in multi-material FFF/FDM, we propose the comparison of different processes: post-processing with acetone immersion, surface activation during printing with Acetone, surface activation during printing with tetrahydrofuran, post-processing annealing, and connection of printed parts with tetrahydrofuran.Modifying the 3D printing process improved the quality of the adhesive bond between the two different polymers. Activation of the surface with THF is the treatment method recommended by the authors due to the low impact on the deformation/degradation of the object.In the study, adhesion was considered in relation to the circular pattern of surface development. Further analysis should include other surface development patterns and changes in printing parameters, e.g. process temperatures and layer application speed.3D printing with multi-materials, such as PLA biopolymer and thermoplastic polyurethane, allows for the creation of flexible connections. The strengthening of the biopolymer broadens the possibilities of using polylactide. Examples of applications include: automotive (elements, where flexible TPU absorbs vibrations and protects PLA from cracking), medicine (prostheses with flexible elements ensuring mobility in the joints).Multi-material printing is a new trend in 3D printing research, and this research is aimed at promoting the use and expanding the possibilities of using PLA biopolymer.

We present development of multi-material Fused deposition modeling (FDM) 3D printer. Most of the FDM type of 3d printers are able to print plastics such as ABS, PLA and Nylon in a single run. In order to overcome this problem, we have developed an affordable multi-material 3D printer that can print an object with a different kind of soft and hard material in a single run. The combination of printing soft and hard materials in a single run will open the doors to various applications such as soft robotics, actuators and sensors. One of the main printing materials for this printer is the conductive based soft filament made by our laboratory "Soft & Wet Matter Engineering Laboratory", along with other different soft filament and hard plastic filament to create a fully functional, multi-material printer to print objects in a single printing run with greater diversity and lower cost than any other single material printer. We have developed a single screw-based extruder 3D printer extruder type 3D printer that can print both soft and hard materials. Instead of using a filament to 3D print objects, we have used pellets of different materials, including carbon-based conductive composites. Such type of single screw base extruder 3D printer will be a new addition to the additive manufacturing process.

Multi-material Direct Ink Writing 3D Food Printing using Multi-channel Nozzle

Primary and Secondary Recycling of Thermoplastics: A Review

Fused Deposition Modeling (FDM) is a popular style of additive manufacturing (AM) where a 3D object is fabricated by a molten material deposited into successive 2D layers. Multi-material 3D printing is particularly challenging due to poor adhesion between dissimilar plastics. The majority of currently available multi-material printers use separate nozzles for each material. Therefore, they are incapable of producing devices made of functionally gradient materials (FGM). FGM is a special class of engineering material exhibiting spatially inhomogeneous content, which tailors the material for specific functional and performance requirements. In this work, we present the design and characterization of a bi-material co-extruder system. This specialized extruder is capable of printing two thermoplastic materials through a single nozzle and is also capable of altering the composition of the deposited material while printing. It is also exceptional in that the internal structure of the hot end can be easily accessed and is a more adaptable design than previously reported co-extruders. The extruder was installed on a Geeetech PI3 Pro C printer. Then, some simple structures were printed using pairs of materials including polylactic acid (PLA), acrylonitrile butadiene styrene (ABS) and high impact polystyrene (HIPS). Without any internal mixing features, the composition of the output (for instance, different colored PLA) was determined from microscopic images of cross-section of the extrudates.

The adhesion in 3D-printed composites between dissimilar layers was investigated in response to demand for multimaterial prints. Previously, we reported on the thermal properties for printability of thermoplastic polyurethane (TPU) blends~\cite{harris001}. Here, we report the qualitative and quantitative adhesion properties between commonly-printed materials, acrylonitrile butadiene styrene (ABS) or acrylonitrile styrene acrylate (ASA), and flexible TPU. ASTM D1002/D3164 lap shear adhesion tests were performed to measure the adhesion strength between the acrylonitrile plastics and TPU, finding values of $4.7~\mathrm{MPa}$ between ABS and TPU and $6.4~\mathrm{MPa}$ between ASA and TPU. These values are on the same order of magnitude of reported commercial adhesives, suggesting that multimaterial 3D printing can provide an automated, industrially-relevant alternative to handmade fabrication. SEM images are reported to show the quality of adhesion between materials in the composites. These images expose qualitative differences in the interlayer adhesion between materials in the composites that depend on the \textit{order of printing}. Thermal (DSC and TGA) and rheological characterizations were performed to investigate the processing conditions of the extruded plastics while they are in their melt states. The role of viscosity is shown to have major implications on the adhesion, as materials with lower viscosities displayed improved filling of the interlayer voids and increased strength of adhesion.

Despite great progress in four-dimensional (4D) printing, i.e. three-dimensional (3D) printing of active (stimuli-responsive) materials, the relatively low actuation force of the 4D printed structures often impedes their engineering applications. In this study, we use multimaterial inkjet 3D printing technology to fabricate shape memory structures, including a morphing wing flap and a deployable structure, which consist of active and flexible hinges joining rigid (non-active) parts. The active hinges, printed from a shape memory polymer (SMP), lock the structure into a second temporary shape during a thermomechanical programming process, while the flexible hinges, printed from an elastomer, effectively increase the actuation force and the load-bearing capacity of the printed structure as reflected in the recovery ratio. A broad range of mechanical properties such as modulus and failure strain can be achieved for both active and flexible hinges by varying the composition of the two base materials, i.e. the SMP and the elastomer, to accommodate large deformation induced during programming step, and enhance the recovery in the actuating step. To find the important design parameters, including local deformation, shape fixity and recovery ratio, we conduct high fidelity finite element simulations, which are able to accurately predict the nonlinear deformation of the printed structures. In addition, a coupled thermal-electrical finite element analysis was performed to model the heat transfer within the active hinges during the localized Joule heating process. The model predictions showed good agreement with the measured temperature data and were used to find the major parameters affecting temperature distribution including the applied voltage and the convection rate.

In 1997, the European Communities Confederation of Clinical Chemistry and Laboratory Medicine (EC4) set up a Register for European Specialists in Clinical Chemistry and Laboratory Medicine. The operation of the Register is undertaken by a Register Commission (EC4RC). During the last 10 years, more than 2000 specialists in Clinical Chemistry and Laboratory Medicine have joined the Register. In 2007, EC4 merged with the Federation of European Societies of Clinical Chemistry and Laboratory Medicine (FESCC) to form the European Federation of Clinical Chemistry and Laboratory Medicine (EFCC). A Code of Conduct was adopted in 2003 and a revised and updated version, taking account particularly of the guidelines of the Conseil Européen des Professions Libérales (CEPLIS) of which EFCC is a member, is presented in this article. The revised version was approved by the EC4 Register Commission and by the EFCC Executive Board in Paris on 6 November, 2008.

Article Congress of Clinical Chemistry and Laboratory Medicine / 10th Annual Meeting of the German Society for Clinical Chemistry and Laboratory Medicine (DGKL), Dresden, Germany, 23rd–26th October, 2013*) was published on October 1, 2013 in the journal Clinical Chemistry and Laboratory Medicine (CCLM) (volume 51, issue 10).

Fibre jamming is a new and understudied soft robotic mechanism that has previously found success in stiffness-tunable arms and fingers. However, to date researchers have not fully taken advantage of the freedom offered by contemporary fabrication techniques including multi-material 3D printing in the creation of fibre jamming structures. In this research, we present a novel, modular, multi-material, 3D printed, fibre jamming tendon unit for use in a stiffness-tunable compliant robotic ankle, or Jammkle. Its multimaterial printed design offers unparalleled design freedom, enabling application specific tendon design. We develop analytical and finite element models of the tendon unit, showing good agreement with experimental data and numerically explore the design space. Finally, we demonstrate a practical application by integrating multiple tendon units into a robotic ankle and perform extensive testing and characterisation. We show that the Jammkle outperforms comparative leg structures in terms of compliance, damping, and slip prevention.

The European Communities Confederation of Clinical Chemistry and Laboratory Medicine (EC4) opened a Register for European Specialists in Clinical Chemistry and Laboratory Medicine in 1997. The operation of the Register is undertaken by a Register Committee (EC4RC). During the last 6 years more than 1500 specialists in clinical chemistry and laboratory medicine have joined the Register. In this article a Code of Conduct for Registrants which was approved at the EC4 Register Committee meeting in Amsterdam, 8 November 2003 is presented.

Laboratory diagnostics develop through different phases that span from test ordering (pre-preanalytical phase), collection of diagnostic specimens (preanalytical phase), sample analysis (analytical phase), results reporting (postanalytical phase) and interpretation (post-postanalytical phase). Although laboratory medicine seems less vulnerable than other clinical and diagnostic areas, the chance of errors is not negligible and may adversely impact on quality of testing and patient safety. This article, which continues a biennial tradition of collective papers on preanalytical quality improvement, is aimed to provide further contributions for pursuing quality and harmony in the preanalytical phase, and is a synopsis of lectures of the third European Federation of Clinical Chemistry and Laboratory Medicine (EFLM)-Becton Dickinson (BD) European Conference on Preanalytical Phase meeting entitled 'Preanalytical quality improvement. In pursuit of harmony' (Porto, 20-21 March 2015). The leading topics that will be discussed include unnecessary laboratory testing, management of test request, implementation of the European Union (EU) Directive on needlestick injury prevention, harmonization of fasting requirements for blood sampling, influence of physical activity and medical contrast media on in vitro diagnostic testing, recent evidence about the possible lack of necessity of the order of draw, the best practice for monitoring conditions of time and temperature during sample transportation, along with description of problems emerging from inappropriate sample centrifugation. In the final part, the article includes recent updates about preanalytical quality indicators, the feasibility of an External Quality Assessment Scheme (EQAS) for the preanalytical phase, the results of the 2nd EFLM WG-PRE survey, as well as specific notions about the evidence-based quality management of the preanalytical phase.

Biological variation (BV) data have many fundamental applications in laboratory medicine. At the 1st Strategic Conference of the European Federation of Clinical Chemistry and Laboratory Medicine (EFLM) the reliability and limitations of current BV data were discussed. The EFLM Working Group on Biological Variation is working to increase the quality of BV data by developing a European project to establish a biobank of samples from healthy subjects to be used to produce high quality BV data. The project involved six European laboratories (Milan, Italy; Bergen, Norway; Madrid, Spain; Padua, Italy; Istanbul, Turkey; Assen, The Netherlands). Blood samples were collected from 97 volunteers (44 men, aged 20-60 years; 43 women, aged 20-50 years; 10 women, aged 55-69 years). Initial subject inclusion required that participants completed an enrolment questionnaire to verify their health status. The volunteers provided blood specimens once per week for 10 weeks. A short questionnaire was completed and some laboratory tests were performed at each sampling consisting of blood collected under controlled conditions to provide serum, K2EDTA-plasma and citrated-plasma samples. Samples from six out of the 97 enroled subjects were discarded as a consequence of abnormal laboratory measurements. A biobank of 18,000 aliquots was established consisting of 120 aliquots of serum, 40 of EDTA-plasma, and 40 of citrated-plasma from each subject. The samples were stored at -80 °C. A biobank of well-characterised samples collected under controlled conditions has been established delivering a European resource to enable production of contemporary BV data.

The improving quality of laboratory testing requires a deep understanding of the many vulnerable steps involved in the total examination process (TEP), along with the identification of a hierarchy of risks and challenges that need to be addressed. From this perspective, the Working Group "Laboratory Errors and Patient Safety" (WG-LEPS) of International Federation of Clinical Chemistry and Laboratory Medicine (IFCC) is focusing its activity on implementation of an efficient tool for obtaining meaningful information on the risk of errors developing throughout the TEP, and for establishing reliable information about error frequencies and their distribution. More recently, the European Federation of Clinical Chemistry and Laboratory Medicine (EFLM) has created the Task and Finish Group "Performance specifications for the extra-analytical phases" (TFG-PSEP) for defining performance specifications for extra-analytical phases. Both the IFCC and EFLM groups are working to provide laboratories with a system to evaluate their performances and recognize the critical aspects where improvement actions are needed. A Consensus Conference was organized in Padova, Italy, in 2016 in order to bring together all the experts and interested parties to achieve a consensus for effective harmonization of quality indicators (QIs). A general agreement was achieved and the main outcomes have been the release of a new version of model of quality indicators (MQI), the approval of a criterion for establishing performance specifications and the definition of the type of information that should be provided within the report to the clinical laboratories participating to the QIs project.