Generative Artificial Intelligence for Biology: Toward Unifying Models, Algorithms, and Modalities

Diffusion models (DMs) are a class of generative machine learning methods that sample a target distribution by transforming samples of a trivial (often Gaussian) distribution using a learned stochastic differential equation. In standard DMs, this is done by learning a "score function" that reverses the effect of adding diffusive noise to the distribution of interest. Here we consider the generalisation of DMs to lattice systems with discrete degrees of freedom, and where noise is added via Markov chain jump dynamics. We show how to use tensor networks (TNs) to efficiently define and sample such "discrete diffusion models" (DDMs) without explicitly having to solve a stochastic differential equation. We show the following: (i) by parametrising the data and evolution operators as TNs, the denoising dynamics can be represented exactly; (ii) the auto-regressive nature of TNs allows to generate samples efficiently and without bias; (iii) for sampling Boltzmann-like distributions, TNs allow to construct an efficient learning scheme that integrates well with Monte Carlo. We illustrate this approach to study the equilibrium of two models with non-trivial thermodynamics, the d=1 constrained Fredkin chain and the d=2 Ising model.

The contemporary scope of nuclear nephrology extends from non-imaging techniques for glomerular filtration rate calculation through dynamic renal scintigraphy and cortical imaging with planar or single photon emission computed tomography (SPECT) approaches to emerging applications in positron emission tomography (PET) renography and theranostics-based renal toxicity risk assessment. Artificial intelligence (AI) shares a long history with nuclear nephrology that started with expert systems and statistical machine learning (ML) approaches, transitioned through feed forward neural networks (FFNN), landed with convolutional neural networks (CNNs) and deep learning (DL), and has emerging opportunities across the gamut of generative AI like large language models (LLMs), diffusion models, generative adversarial networks (GANs) and multimodal models like vision language models (VLMs). A range of AI tools across the nuclear nephrology ecosystem describe bespoke AI algorithms, commercial AI products, embedded AI tools from vendors, general-purpose and cross-domain AI frameworks. Applications in clinical workflow, research and development, and imaging are explored, highlighting the potential of AI in detection, classification, segmentation, prediction, data analysis and image enhancement. Emerging AI opportunities from generative AI, LLMs, VLMs, and segmentation foundation models such as the SAM, offer exciting multi-modal, few-shot learning that may re-imagine nuclear nephrology. There remains the need for considerable development and validation for widespread clinical utility of AI opportunities, and the need for consideration of ethical limitations and social justice.

Technologically advanced artificial intelligence (AI) is making a significant contribution to strengthening national security. AI algorithms facilitate the processing of vast amounts of information, increasing the speed and accuracy of decision-making. Artificial intelligence and machine learning (AI/ML) are crucial for state and integrated hybrid attacks and protecting new threats in cyberspace. Existing AI capabilities have significant potential to impact national security by leveraging existing machine learning technology for automation in labor-intensive activities such as satellite imagery analysis and defense against cyber attacks. This article examines selected aspects of the impact of artificial intelligence on enhancing a state’s ability to protect its interests and its citizens., artificial intelligence through the use of neutron networks, predictive analytics and machine learning algorithms enables security agencies to analyse vast amounts of data and identify patterns indicative of potential threats. Integrating artificial intelligence into surveillance, border control and threat assessment systems enhances the ability to respond preemptively to security challenges. In addition, artificial intelligence algorithms facilitate the processing of vast amounts of information, increasing the speed and accuracy of decision-making by police authorities. The rapid development of AI raises a number of questions for its use in securing not only national security but protecting all citizens. In particular, it is worth answering the question How does artificial intelligence affect national security and clarifying the issue of how law enforcement agencies can use artificial intelligence to maximise the benefits of the new technology in terms of security and protecting communities from rising crime. The analysis is based on a descriptive method in describing the phenomenon; by explaining the concepts and applications of artificial intelligence to determine its role in the national security sphere. An analysis of the usefulness of artificial intelligence in particular in police operations is undertaken, with the aim of defending the thesis that, despite some threats to the protection of human rights from AI, it is becoming the best tool in the fight against all types of crime in the country. Technological advances in AI can also have many positive effects for law enforcement, and useful for law enforcement agencies, for example in facilitating the identification of persons or vehicles, predicting trends in criminal activities, tracking illegal criminal activities or illegal money flows, flagging and responding to fake news. Artificial intelligence (AI) has emerged as one of the biggest threats to information security, but efforts are being made to mitigate this new threat, but also to find solutions on how AI can become an ally in the fight against cyber-security, crime and terrorist threats. Artificial intelligence algorithms search huge datasets of communication traffic, satellite images and social media posts to identify potential cyber security threats, terrorist activities and organized crime. It is advisable, when analyzing the opportunities and threats that AI poses to national and public security, to gain a strategic advantage in the context of rapid technological change and also to manage the many risks associated with AI. The conclusion highlights the impact of AI on national security, creating a range of new opportunities coupled with challenges that government agencies should be prepared for in addressing ethical and security dilemmas. Furthermore, AI improves predictive analytics, thereby enabling security agencies to more accurately anticipate potential threats and enhance their preparedness by identifying vulnerabilities in the national security infrastructure

<sec><title>Objective</title> As urban design faces increasing demands for contextual responsiveness, iterative optimization, and data-informed reasoning, integrating artificial intelligence into the design process has gained renewed relevance. Among emerging technologies, generative artificial intelligence (GAI) shows strong potential for automating content creation and simulating spatial configurations. This research provides a comprehensive review of recent developments in the application of GAI to urban design. The research identifies representative technical pathways, their respective intervention stages, and the functional mechanisms by which generative models are reshaping the design workflow. This research presents a structured, theory-informed synthesis of how different generative models contribute to tasks such as intention modeling, spatial reasoning, and performance-driven design. Building on design thinking and a descriptive lens informed by the technology acceptance model (TAM), the research examines how model type, data modality, and task characteristics affect GAI’s functional role, usability, and acceptance. Particular attention is given to mapping deployment forms, from isolated tools to coordinated multi-model workflows, and to characterizing cross-cutting challenges of controllability, transparency, and contextual adaptability in urban design settings. </sec><sec><title>Methods</title> Following the PRISMA protocol, the research conducts a multi-stage literature review combining automated search and expert screening. A total of 125 peer-reviewed articles and high-impact preprints are selected from Web of Science, CNKI, arXiv, and selected industry sources, covering the period from 2014 to July 2025. Search terms such as “generative AI”, “AIGC”, “GAN”, “diffusion model”, “variational autoencoder”, “autoregressive model”, “large language model”, and urban-related keywords are used in various combinations. Based on the collected literature, four types of generative models are summarized as image-driven, language-driven, structure-driven, and feedback-optimized models, according to their application characteristics in urban design tasks. These types are aligned with four stages of the design process: preliminary analysis, scheme generation, evaluation and decision-making, and outcome expression. On this basis, a two-dimensional framework to examine how different GAI pathways intervene across tasks is formed. To refine the mapping, each design stage is further broken down into three representative sub-tasks. Preliminary analysis includes public demand analysis, urban data enhancement, case/task framing, and spatial element recognition. Scheme generation covers design intention modeling, spatial layout generation, and 3D form construction. The evaluation and decision-making stage includes multi-objective optimization, scheme evaluation, and scenario prediction. The final expression stage involves textual documentation, 2D representation, and visual rendering. A quantitative analysis is also conducted to show the distribution of model types over design stages, identify common combinations, and trace the evolution of research focus over time. TAM informs a descriptive synthesis of perceived usefulness (PU) and perceived ease of use (PEU) across model types to illuminate adoption patterns. </sec><sec><title>Results</title> The findings reveal that GAI models are increasingly integrated into urban design workflows but exhibit uneven adoption across task types and modalities. Image-driven models dominate in both early-stage analysis and final visual representation due to their high interpretability, usability, and compatibility with existing design practices. Language-driven models are commonly used in public demand analysis, participatory planning, and scenario scripting, enabled by the rise of large language models (LLMs) such as ChatGPT and DeepSeek. Structure-driven models, though less prevalent, show promise in generating street networks, land-parcel layouts, and spatial typologies using graph-based logic. Feedback-optimized models, which rely on reinforcement learning, evolutionary algorithms, and performance simulation are the least adopted, but demonstrate strong potential in multi-objective optimization and iterative decision-making. Recent research indicates an increasing use of multi-model workflows, such as text-to-image pipelines integrated with urban simulation or feedback loops. While GAI applications increasingly support design iteration, their adoption is heavily influenced by the controllability, explainability, and contextual adaptability of models. PU and PEU vary significantly by model type, with image-driven models rated highest and structure-driven and feedback-optimized models facing usability challenges due to complexity and low transparency. </sec><sec><title>Conclusion</title> Although GAI has demonstrated broad applicability across the urban design process, current implementations are largely procedural and auxiliary in nature. Most models recombine existing inputs rather than construct original logic, and few possess autonomous reasoning or normative awareness. This limits their role to content augmentation rather than conceptual guidance in design development. Moreover, issues such as opaque decision logic, lack of domain-specific knowledge embedding, and poor adaptability to local planning norms hinder practical adoption. Addressing these challenges requires multi-level efforts: 1) Construct structured, regionally grounded urban design datasets; 2) improve model interpretability, controllability, and responsiveness to professional input; and 3) develop modular, multi-model systems that support seamless interaction across design stages. Human – AI collaboration mechanisms — especially those based on iterative prompts and semantic feedback-must be enhanced to enable AI not just as a tool, but as an active design partner. This review offers a comprehensive reference for scholars and practitioners seeking to understand how GAI is reshaping the logic, structure, and agency of urban design. </sec>

This review examines the rapidly expanding landscape of Generative Artificial Intelligence (GenAI) in healthcare, focusing on how models such as GANs, VAEs, diffusion models, and large language models are being explored across medical imaging, clinical documentation, synthetic data generation, drug discovery, and decision-support workflows. Despite GenAI's growing influence, persistent challenges, including limited annotated datasets, concerns over model generalizability, privacy risks, and the opacity of generative architectures, underscore the need for careful evaluation and governance. Accordingly, this study aims to map current applications, assess methodological and ethical constraints, and identify future research opportunities. Using a structured search across ScienceDirect, Scopus, and other sources, the study follows a structured narrative review complemented by quantitative descriptive analysis of the literature. The review also adopts PRISMA-guided screening and standardized data extraction, the review synthesizes evidence from 110 studies published up to October 2025. The findings indicate that the literature frequently reports improvements in imaging quality, data augmentation, molecular modeling workflows, and clinical documentation through generative approaches, particularly in technically constrained settings; however, evidence of clinically validated impact remains uneven across domains. While issues of bias, hallucination, and limited interpretability persist as significant obstacles, imaging-focused applications appear comparatively more mature than decision-support and patient-level modeling tasks. Across domains, diffusion models are commonly associated with higher visual fidelity in biomedical image generation, whereas LLMs demonstrate promise in narrative-oriented tasks but require stronger factual grounding and external verification mechanisms. Overall, the evidence suggests that GenAI’s potential in healthcare is highly context-dependent and contingent upon robust validation frameworks, transparent governance, and human-in-the-loop oversight. The review concludes that responsible integration of GenAI—guided by ethical, legal, and clinical safeguards, will be essential for ensuring safe, equitable, and sustainable adoption in healthcare research, delivery, and policy.

Large language models (LLMs) are increasingly adopted in educational contexts to provide personalized support to students and teachers. The unprecedented capacity of LLM‐based applications to understand and generate natural language can potentially improve instructional effectiveness and learning outcomes, but the integration of LLMs in education technology has renewed concerns over algorithmic bias, which may exacerbate educational inequalities. Building on prior work that mapped the traditional machine learning life cycle, we provide a framework of the LLM life cycle from the initial development of LLMs to customizing pre‐trained models for various applications in educational settings. We explain each step in the LLM life cycle and identify potential sources of bias that may arise in the context of education. We discuss why current measures of bias from traditional machine learning fail to transfer to LLM‐generated text (eg, tutoring conversations) because text encodings are high‐dimensional, there can be multiple correct responses, and tailoring responses may be pedagogically desirable rather than unfair. The proposed framework clarifies the complex nature of bias in LLM applications and provides practical guidance for their evaluation to promote educational equity. Practitioner notes What is already known about this topic The life cycle of traditional machine learning (ML) applications which focus on predicting labels is well understood. Biases are known to enter in traditional ML applications at various points in the life cycle, and methods to measure and mitigate these biases have been developed and tested. Large language models (LLMs) and other forms of generative artificial intelligence (GenAI) are increasingly adopted in education technologies (EdTech), but current evaluation approaches are not specific to the domain of education. What this paper adds A holistic perspective of the LLM life cycle with domain‐specific examples in education to highlight opportunities and challenges for incorporating natural language understanding (NLU) and natural language generation (NLG) into EdTech. Potential sources of bias are identified in each step of the LLM life cycle and discussed in the context of education. A framework for understanding where to expect potential harms of LLMs for students, teachers, and other users of GenAI technology in education, which can guide approaches to bias measurement and mitigation. Implications for practice and/or policy Education practitioners and policymakers should be aware that biases can originate from a multitude of steps in the LLM life cycle, and the life cycle perspective offers them a heuristic for asking technology developers to explain each step to assess the risk of bias. Measuring the biases of systems that use LLMs in education is more complex than with traditional ML, in large part because the evaluation of natural language generation is highly context‐dependent (eg, what counts as good feedback on an assignment varies). EdTech developers can play an important role in collecting and curating datasets for the evaluation and benchmarking of LLM applications moving forward.

Artificial Intelligence (AI) is a rapidly evolving field that is influencing every aspect of life. Generative AI (GenAI) as a sub-branch of AI is used to create content in various formats such as text, images, video, and audio. This paper discusses the implications of GenAI for Open Educational Practices (OEP), highlighting the potential affordances and challenges. GenAI can address the challenges within the OEP by leveraging openness and ethical use. GenAI&rsquo;s &ldquo;generative&rdquo; nature and human-like language capability can provide resources such as course material, activities, examples, questions, assessment, and learning outcomes in the context of OEP. With machine learning and deep learning infrastructure, it can support the discoverability and accessibility of open resources by increasing the metadata quality. GenAI can automatically score student assignments, answer their questions, and provide instant feedback to address the lack of interaction and feedback that arises due to the large number of students, especially in massive open online courses (MOOC). On the other hand, GenAI brings challenges such as data privacy and security, copyright, biased outputs, and the generation of false information. The conclusions emphasize the importance of a nuanced approach that considers not only the advantages but also the risks associated with adopting GenAI in the OEP world. Researching and developing how to apply these technologies to education is important to shape the future of education.

Artificial intelligence (AI) and language models such as ChatGPT-4 (Generative Pretrained Transformer) have made tremendous advances recently and are rapidly transforming the landscape of medicine. Cardiology is among many of the specialties that utilize AI with the intention of improving patient care. Generative AI, with the use of its advanced machine learning algorithms, has the potential to diagnose heart disease and recommend management options suitable for the patient. This may lead to improved patient outcomes not only by recommending the best treatment plan but also by increasing physician efficiency. Language models could assist physicians with administrative tasks, allowing them to spend more time on patient care. However, there are several concerns with the use of AI and language models in the field of medicine. These technologies may not be the most up-to-date with the latest research and could provide outdated information, which may lead to an adverse event. Secondly, AI tools can be expensive, leading to increased healthcare costs and reduced accessibility to the general population. There is also concern about the loss of the human touch and empathy as AI becomes more mainstream. Healthcare professionals would need to be adequately trained to utilize these tools. While AI and language models have many beneficial traits, all healthcare providers need to be involved and aware of generative AI so as to assure its optimal use and mitigate any potential risks and challenges associated with its implementation. In this review, we discuss the various uses of language models in the field of cardiology.

Generative artificial intelligence (GAI), a specialized branch of artificial intelligence, has developed as a dynamic discipline that drives innovation and creativity across several domains. It is concerned with creating models that can autonomously produce novel text, images, videos, music, 3D, code, and more. GAI is distinguished by its capacity to acquire knowledge from extensive datasets, identify recurring patterns, capture distributions, and produce novel content demonstrating similar features. This review presents a comprehensive historical overview of the development and progression of GAI techniques over the years. Various essential methodologies employed in developing GAI are also discussed, including Generative Adversarial Networks (GANs), Variational AutoEncoders (VAEs), transformers, and diffusion models. Moreover, a detailed overview of the technologies used in GAI is provided for generating images, videos, music, code, and text, such as ChatGPT, DALL-E, Midjourney, Claude, Bard, GitHub Copilot, and others. The research subsequently introduces the different datasets used to train the GAI models and the evaluation metrics for evaluating their performances. Ultimately, the research investigates the diverse applications of GAI across various domains, challenges, and ethical implications.

HKUST Electronic Theses Image restoration, editing, and assessment with generative artificial intelligence by Liya Ji thesis 2024 1 online resource (xiii, 92 pages) : illustrations (chiefly color) Generative Artificial Intelligence (AI), such as diffusion models and large language models, can…Read more ›

Harnessing Generative Artificial Intelligence to Improve Efficiency Among Urologists: Welcome ChatGPT.

Artificial General Intelligence (AGI) represents a significant leap in the field of artificial intelligence, defined by its ability to perform any intellectual task that a human can. Unlike narrow AI, which is task-specific, AGI is characterized by versatility, adaptability, autonomy, and reasoning capabilities. This comprehensive review explores the defining features of AGI, including its cognitive flexibility and capacity for autonomous decision-making and problem-solving. The current state of AGI research is examined, highlighting key areas such as cognitive architectures, neuroscience-inspired AI, and machine learning and deep learning advancements. The review also addresses the technical challenges and ethical considerations associated with AGI development, including potential impacts on employment, privacy, and security, as well as the necessity for robust safety and control measures. Looking ahead, the potential benefits of AGI in various domains, such as healthcare, climate change, education, and economic growth, are discussed. Finally, the importance of establishing ethical frameworks and governance structures to guide AGI development and usage is emphasized. By addressing these challenges and fostering collaboration among scientists, policymakers, and society, AGI can be developed and deployed to benefit humanity as a whole.

The amazing technological developments in Artificial Intelligence (AI) cast important questions for all scholars. Prominently, can AI replace human beings in all fields, which might be a double-edged weapon? Because, from one hand, it will have positive sides. For example, it will give more accurate results, increase production, save time and energy, make better use of natural resources, and find creative solutions for problems…etc. But, if it replaced human beings, AI will also have negative effects on them; varying from the socio-economic effects to the psychological and/or even the political ones. The control of the machines on most (or even all) fields of life may be startling. Moreover, can AI be misused by evil persons or competitive parties? These persons or parties may easily forge photos, videos, voices of their competitors. They are able to exploit fake documentations against their enemies using the powerful intelligent abilities of AI programmes. Hence, can mankind be ready to face AI future consequences? Will it be smarter than humans because of its surprising capabilities for quick self-learning? One of the astonishing developments of AI is Generative Artificial Intelligence (GAI), which has several language models. These models can do highly innovative tasks like writing neat poems of different styles, about numerous subjects, or from various literary ages. This complicated task poses new significant questions: Will GAI replace the poets? Will it put an end to human innovation? Yet, other sides of the matter make it dubious and controversial; as poetry mainly is the product of human emotions, feelings, and experiences. Thus, this research paper tries to find answers to these questions, differentiate between human-produced poetry and that generated by AI models, trace the effect of the GAI models on the production of poetry, and show how GAI turned from an imitator to an author. An example of this kind of poetry is the collection entitled: I Am Code: An Artificial Intelligence Speaks (2023). Samples of these poems are examined and discussed.

Artificial intelligence (AI) has emerged as a transformative tool in the diagnosis and management of gastrointestinal (GI) and liver diseases. In clinical practice AI consists of overlapping technologies such as machine learning (ML), deep learning, natural language processing, computer vision, and generative AI. ML is a computer learning system that can provide insight into disease risk factors and phenotypes. Deep learning is an advanced and complex form of ML, structured with different levels of specific algorithms known as convolutional neural networks that can rapidly and accurately process unstructured, high-dimensional data, such as texts, images, and waveforms. Natural language processing is dedicated to facilitating interactions between computers and humans using natural language and helps to analyze, understand, and derive actionable information from unstructured healthcare data, including electronic health records, clinical notes, medical literature, and patient-generated content. Computer vision focuses on enabling computers to see and interpret images and videos and serves as an augmentation tool for endoscopists, improving accuracy and decreasing procedural time. Generative AI is capable of creating new forms of content by learning from a large body of data in the form of text, audio, images, or video and includes large language models. AI has been used in several GI diseases such as esophageal neoplasia, gastric cancer, Helicobacter pylori infection, gastritis, GI stromal tumors, colorectal polyps, inflammatory bowel disease, irritable bowel syndrome, GI bleeding, and pancreatobiliary diseases. The potential applications of AI in liver diseases encompass a variety of conditions such as liver masses, metabolic dysfunction-associated steatotic liver disease, viral hepatitis, cirrhosis, and liver transplantation. This review discussed the common terminologies and the current status of AI in gastroenterology and hepatology, exploring its applications and ethical issues.

Recent advancements in Artificial Intelligence (AI) and Machine Learning (ML) technology have brought on substantial strides in predicting and identifying health emergencies, disease populations, and disease state and immune response, amongst a few. Although, skepticism remains regarding the practical application and interpretation of results from ML-based approaches in healthcare settings, the inclusion of these approaches is increasing at a rapid pace. Here we provide a brief overview of machine learning-based approaches and learning algorithms including supervised, unsupervised, and reinforcement learning along with examples. Second, we discuss the application of ML in several healthcare fields, including radiology, genetics, electronic health records, and neuroimaging. We also briefly discuss the risks and challenges of ML application to healthcare such as system privacy and ethical concerns and provide suggestions for future applications.