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  • Grading iris color of post‐mortem human eyes
  • https://doi.org/10.1111/j.1755-3768.2016.0670Copy DOI Icon

Grading iris color of post‐mortem human eyes

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Abstract

PurposeIris colour encompasses a continuum from pale blue to very dark brown and is usually classified via a descriptive three colour scale: blue, green‐hazel or brown. Digital imaging technologies provide an opportunity to quantify iris colour, and are increasingly used for studies of genetic variations in iris colour. We explored the use of digital imaging and colour space information for grading of iris colour in post‐mortem eyes, including eyes with choroidal naevi.MethodsPost‐mortem adult human irises (n = 25) were examined and photographed using a Jenoptik digital camera and ProgResCaptureProv2.8.9 software. Standard lighting (colour temperature) and parameters for imaging were established and used for all specimens. Iris colour was graded (n = 5) (using a nine‐category system; Mackey et al. Clin Exp Ophthalmol, 2011). We also developed a method in Photoshop to express iris colour as Hue in the Hue, Saturation, Brightness (HSB) colour space, for comparison with the category grading.ResultsUsing the nine‐category grading end‐point grades (light blue and dark brown) were consistently graded. Intermediate grades were categorised differently for some observers, usually with adjacent categories. Green irises were not observed in this small series. Digital imaging using standard iris images and an averaging filter, provided colour information (Hue) for each iris. This allowed discrimination of iris colour compared to category grading.ConclusionsAs expected category‐grading was not always consistent between observers. We developed a digital imaging approach using HSB color space to give a H value for each iris. We are exploring the utility of processing functions such as gaussian blur. This approach provides a standard iris colour for post‐mortem tissue and will be used for comparison with fundus colour.

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