Is hyperleptinemia a risk factor for hypertension?

Background.Hypertension is frequently found in obesity, although the mechanisms for obesity related hypertension are still unclear. Several studies showed the role of endothelin-1 in the development of hypertension asso-ciated with obesity. More recent studies showed the possibility for the role of leptin in obesity related hypertension. In vitro study with human umbilical vein endothelial cells (HUVECs) showed correlation between leptin and endothelin-1. The aim of this study was to clarify the relation-ship between leptin, body mass index (BMI), blood pressure (BP) and plasma endothelin-1 in obese hypertensive subjects. Methods.A cross sectional study was performed at National Cardiovas-cular Centre, Harapan Kita Jakarta, which included 80 Indonesian men. The subjects were divided into lean hypertensive and obese hypertensive. The plasma immunoreactive ET-1 and leptin were determined by ELISA method. Results.ET-1 level and leptin level were significantly higher in obese hy-pertensive subjects compared with lean hypertensive subjects. There are significant correlation between endothelin level with BMI (r = 0.32; p < 0.005). There are also significant correlation between leptin level with BMI (r = 0.533; p <0.01), but there are no correlation between plasma leptin and endothelin-1 level in obese hypertensive men. Conclusions.Leptin and ET-1 level were significantly increase in obese hypertensive subjects compared with lean hypertensive subjects. There is no correlation between leptin and ET-1 level in obese hypertensive subjects.

Pro-inflammatory adipocytokines, oxidative stress, insulin, Zn and Cu: Interrelations with obesity in Egyptian non-diabetic obese children and adolescents

Background Obstructive sleep apnea (OSA) is characterized by a combination of structural issues in the upper airway and imbalances in the respiratory control system. While numerous studies have linked OSA with obesity, it remains uncertain whether leptin, a hormone associated with fat, plays a role in the functional and anatomical defects that lead to OSA. Therefore, the aim of this study was to investigate whether leptin levels could be used as a predictor of OSA syndrome (OSAS). Methodology A case-control observational study was conducted, enrolling study participants who reported obesity (BMI > 30) within the range of >30 to <35 kg/m2, along with a short neck and a history of snoring, excessive daytime drowsiness, fatigue, or insomnia. Leptin levels and fasting blood sugar (FBS) were measured in all individuals. Additionally, the study evaluated the severity of OSAS using indicators such as the STOP BANG scores, apnea-hypopnea index, uvula grade score, and Epworth Sleepiness Scale scores. Results A total of 80 participants (40 cases and 40 controls) were included in the study. The mean leptin and FBS levels were significantly higher in cases compared to controls. Moreover, leptin levels exhibited a significant correlation with the severity indices of OSAS. Conclusion The study findings indicate that individuals with higher leptin levels tend to exhibit more severe OSAS symptoms. Furthermore, these elevated leptin levels contribute to the worsening of various OSA symptoms. Larger controlled studies have suggested that pharmacologically restoring the altered leptin levels may serve as a beneficial adjunct to treatment for alleviating OSAS symptoms.

Circulating concentrations of leptin are exceedingly low in severe malnutrition as seen in the acute state of anorexia nervosa (AN). During refeeding therapy plasma leptin levels increase to normal and in some cases peak at values in excess of the BMI of matched controls even before a normal body weight has been achieved. Peak leptin levels are possibly the cause of an increased energy expenditure during this stage of the disorder and might predispose to renewed weight loss (rebound phenomenon). In this study we investigated the role of leptin fluctuations as a prognostic factor of therapeutic success in AN. In 11 anorectic female patients serum leptin levels, BMI and body fat percentage were evaluated in four-week intervals during a conventional refeeding program over three months (group 1). The results of the first two measurements were used to determine a range of increases in leptin levels in relation to increases in BMI. The values between the 25th and 75th percentiles determined the reference range. In a second group of 9 anorectic female patients serum leptin levels, BMI, body fat percentage and the increase in the leptin level in relation to the BMI of each subject were investigated for three months every two weeks. These patients were also treated according to the same conventional refeeding program, but the caloric intake was reduced or increased (+/-250 kcal/d) if the increase in the leptin level, in relation to the increase in the BMI, had exceeded or fallen short of the reference range. During the refeeding therapy every subject of each group experienced increases in serum leptin levels, BMI and body fat percentage. Six subjects of group 1 and six subjects of the second group had an increase in leptin levels in relation to the increase of the BMI out of the reference range at least once. To investigate the therapeutic outcome of leptin monitoring and the following alteration of caloric intake, weight gain of the patients of both groups during the whole treatment was compared. No significant difference was found. Our results probably do not support the findings that high leptin levels predispose to a renewed loss of weight. The outcome in our patients whose caloric intake was modified due to their serum leptin levels was not significantly improved.

Serum leptin and cholesterol values in violent and non-violent suicide attempters

Hypertension is the leading risk factor for death and disability worldwide and a major contributor to premature mortality. Individuals with hypertension often exhibit alterations in lipid and glucose metabolism, further increasing their risk of severe cardiovascular complications. The present study evaluated the lipid profile and blood sugar among hypertensive individuals in Yenagoa, Nigeria. A total of 246 subjects (31-60 years) were recruited for the study. They consisted of 172 hypertensives attending the Cardiology Clinic of Niger Delta University Teaching Hospital (NDUTH) and 74 normotensive control subjects drawn from the staff of NDUTH. Body mass index, fasting blood sugar and lipid profile were measured following standard protocols. The result of this study shows a significantly elevated mean levels of fasting blood sugar (FBS), total cholesterol (TC), triglycerides (TG), and low-density lipoproteins (LDL) among hypertensive subjects compared to the normotensive controls (p&amp;lt;0.05). Fasting blood sugar showed sex and age-dependent variations among the hypertensives. Male hypertensive subjects had a significantly higher fasting blood sugar compared to the female hypertensives (p&amp;lt;0.05). Also, fasting blood sugar increased with age for both hypertensive and normotensive subjects regardless of gender. The current evidence has identified elevated BMI, FBS, TC, TG, and LDL among hypertensive patients receiving medical care at a tertiary healthcare facility in Yenagoa, Nigeria. These findings underscore the significant association between hypertension and metabolic risk factors and recommend routine assessments of these metabolic parameters among hypertensive patients.

To investigate the acute effect of dexamethasone administration on serum leptin levels and the relationships between dehydroepiandrosterone (DHEAS), androstenedione, testosterone and the IGF-I/IGFBP system and leptin levels in healthy elderly humans. In 209 healthy elderly individuals (95 men, 114 women, aged 55-80 years) measurements were made in the fasting state (0800 h) and after an overnight dexamethasone suppression test (1 mg p.o. at 2300 h. Mean leptin levels increased from 6.2 +/- 0.4 (SE) micrograms/l to 7.3 +/- 0.5 (SE) micrograms/l in men and from 18.9 +/- 1.4 (SE) micrograms/l to 23.9 +/- 1.8 (SE) micrograms/l in women after 1 mg dexamethasone overnight ('post treatment')(P < 0.001 for both sexes). There was a significant relationship between post-treatment leptin and dexamethasone levels (men: P = 0.002; women: P < 0.001). The increase in leptin levels after dexamethasone administration was only partially related to the increase in plasma insulin concentrations. Cortisol levels were not related to leptin. In multivariate analyses the relationship between post-treatment leptin and dexamethasone levels remained after adjustment for post-treatment insulin levels, BMI, waist:hip ratio (WHR) and age (men: P < 0.001; women: P = 0.001). Plasma (free and total) IGF-I and IGFBP-3 levels were not related to leptin levels in men or women. IGFBP-1 levels were inversely related to leptin levels (P = 0.02), but this relationship was lost after adjustment for insulin, and/or BMI. In multivariate analyses the relationship between leptin and DHEAS was inverse in women (P = 0.04) (after adjustment for BMI, WHR, insulin and glucose), while there was no relationship between leptin and DHEAS in men. Administration of dexamethasone acutely increased leptin levels within 9 h in this elderly population. This increase was only partly related to changes in circulating insulin concentrations, but was independent of BMI and waist:hip ratio. No relation existed between leptin and (free or total) IGF-I and IGFBP-3 in men or women. Dehydroepiandrosterone was inversely related to leptin in women. These findings suggest a contributory regulatory role for corticosteroids in modulating circulating leptin concentrations in elderly healthy individuals of both sexes, which is at least in part independent of insulin, BMI and waist:hip ratio. Dehydroepiandrosterone might play a role in the gender-specific differences in serum leptin levels.

General Background: Diabetes Mellitus (DM) is a chronic disease characterized by hyperglycemia. Specific Background: A severe complication of DM is diabetic nephropathy, marked by progressive kidney function decline. Blood sugar and urea levels serve as crucial indicators in diagnosing DM and its complications. Knowledge Gap: Limited comparative studies exist on fasting blood sugar and urea levels between diabetes mellitus patients and diabetic nephropathy patients. Aims: This study aims to compare fasting blood sugar levels and urea levels in diabetes mellitus patients and diabetic nephropathy patients. Results: Conducted from April to June 2024, this quantitative, laboratory experimental study sampled 30 diabetes mellitus patients and 30 diabetic nephropathy patients from RSUD dr. Wahidin Sudiro Husodo, Mojokerto City. Using the SPSS Mann-Whitney test, the study found significant differences (p=0.000) in fasting blood sugar levels (125.20±20.348 mg/dL vs. 221.07±65.545 mg/dL) and urea levels (13.30±2.059 mg/dL vs. 54.10±26.699 mg/dL) between the two patient groups. Novelty: This study provides novel insights into the metabolic differences between diabetes mellitus and diabetic nephropathy patients, emphasizing the progressive impact of high blood sugar on kidney function. Implications: The findings underscore the importance of early intervention and continuous monitoring of blood sugar and urea levels to prevent or mitigate diabetic nephropathy in DM patients. Highlights : Significant Differences: The study found significant differences in fasting blood sugar and urea levels between diabetes mellitus and diabetic nephropathy patients. Metabolic Insights: Provides insights into the metabolic impact of high blood sugar on kidney function in diabetic nephropathy patients. Clinical Implications: Highlights the need for early intervention and continuous monitoring to prevent or mitigate diabetic nephropathy in DM patients. Keywords : Diabetes Mellitus, Diabetic Nephropathy, Fasting Blood Sugar Levels, Urea Levels, Kidney Function

Leptin is an anorexigenic hormone well recognized by its role in mediating energy homeostasis. Recently, leptin has been associated with psychiatric disorders and interestingly, leptin treatment has shown antidepressant and anxiolytic effects. We examined the association of leptin levels and leptin (LEP) gene rs3828942 polymorphism with anxiety disorders considering sex differences. A cross-sectional population-based study, including 1067 young adults, of whom 291 presented anxiety disorders diagnosed by the Mini International Neuropsychiatric Interview (MINI 5.0). The rs3828942 polymorphism was genotyped by real-time PCR and ELISA measured leptin levels. Leptin levels were not associated with anxiety disorders after adjusting for sex and body mass index (BMI) [ß = - 0.009 (- 0.090-0.072); p = 0.832]. The distribution of rs3828942 genotypes was not associated with anxiety disorders. However, in a sex-stratified sample, the A-allele of rs3828942 polymorphism was associated with risk for GAD in women even when adjusting for confounding variables [OR = 1.87 (1.17-2.98); p = 0.008]. In a subsample of 202 individuals with GAD and control matched by sex and BMI, results suggest an interaction between genotypes and GAD diagnosis based on leptin levels only in the male group [F (1, 54) = 6.464; p = 0.0139]. Leptin is suggested to be related with the neurobiology of anxiety disorders in a sex-dependent manner since women carrying the A-allele of LEP rs3828942 present a higher risk for GAD, while leptin levels seem to be lower in men with GAD carrying A-allele. Studies on the relationship between leptin polymorphisms and levels are scarce and, therefore, further research is necessary.

The purpose of the present study was to determine the impact of alcohol consumption on the incidence of hypertension. A total of 32,389 male coal mine workers from Kailuan Coal Group in northern China (Kailuan study), free of hypertension, myocardial infarction, stroke, transient ischemia attack and cancer, were followed up every 2 years for 4 years. The average alcohol intake during the past year was reported as baseline. During 4 years of follow-up, 9151 out of 32,389 workers developed hypertension categorically. At the end of the follow-up, the cumulative incidence of hypertension in relation to daily alcoholic intake of none, 1-24, 25-49, 50-99, 100-149 and at least 150 g was 25.03, 28.82, 30.10, 37.07, 40.14 and 42.49%, respectively. After adjusting for age, we found that the relative risk of hypertension in those who were never exposed to alcohol was the lowest, with the group 25-49 g/day being the next. This trend was unchanged after adjustment for age, exercise, smoking status, job type and salt intake. After further adjustment for BMI, history of high cholesterol and diabetes mellitus, a positive, linear association between alcohol consumption and the risk of hypertension was found. Models stratified by baseline SBP (<120 and 120-139 mmHg) or DBP (<80 and 80-89 mmHg) did not alter the trend. The Kailuan study demonstrates that long-term alcohol intake is an independent risk factor of incident hypertension in a large cohort of coal mine workers. Even light-to-moderate alcohol consumption increases the risk of incident hypertension.

Background: Leptin is associated with body weight (BW) regulation in adults and adolescents. Decrease in adiponec- tin in obese adults and adolescents may be associated with the development of obesity and insulin resistance. This study investigated the relationship of plasma leptin and adiponectin levels to changes in BW and body mass index (BMI) over fi ve years among adolescents in Taiwan. Methods: This was a prospective study involving school adolescents aged 12 to 15 years. Demographic data, anthropometric variables, and blood specimens were collected from 1500 adolescents at baseline. Body height, weight and body mass index (BMI) were evaluated at baseline, and after fi ve years in 580 ado- lescents. Plasma leptin and adiponectin levels were measured using radioimmune assay (RIA). Results: Baseline serum levels of leptin and adiponectin were higher in girls than in boys. Baseline plasma leptin levels correlated positively, and adiponectin levels negatively with BW and BMI at baseline and after fi ve years. Plasma leptin levels were negatively as- sociated with fi ve-year changes in BW and BMI both in normal-weight boys (β= -0.57, p < 0.01, and β= -0.25, p < 0.001, respectively) and girls (β= -0.28, p < 0.001, and β= -0.11, p < 0.001, respectively). The correlation between plasma adiponectin levels and fi ve-year change in BW and BMI in both genders was not statistically signifi cant. Conclusions: In Taiwanese adolescents, plasma leptin levels were negatively and signifi cantly associated with the changes in BW and BMI during the fi ve-year follow-up period in both genders, but adiponectin levels were not. Such fi nding suggests that plasma leptin levels may be a negative marker of body weight gain over time.

Objective To explore the level of plasma adiponectin, leptin level in elderly patients with bacterial pneumonia and its correlation with procalcitonin (PCT), C-reactive protein (CRP) and pneumonia severity index (PSI). Methods 32 patients with severe pneumonia (SP group), 30 patients with non-severe pneumonia (NSP group), and 30 patients without pneumonia (healthy control) were included into this study.The levels of adiponectin, leptin, PCT and CRP were detected, and PSI was performed. Results Compared with the healthy control group, the levels of adiponectin in NSP and SP group decreased significantly, the lowest was in SP group; The levels of leptin in NSP and SP group were increased, the highest was in SP group.The levels of adiponectin had positive correlation with PCT, CRP and PSI (r=-0.376、-0.397、-0.410, P<0.05或<0.01), but the levels of leptin had negative correlation with PCT, CRP and PSI (r=0.677、0.353、0.256, P<0.05 or<0.01). Conclusions As an index, adiponectin and leptin can reflect the severity of the infection.The level of adiponectin and leptin can evaluate the severity of elderly patients with bacterial pneumonia. Key words: Adiponectin; Leptin; Elderly; Bacterial pneumonia

BackgroundExperimental and clinical studies have suggested that symbiotics might effectively manage type 2 diabetes mellitus (T2DM) by modulating the intestinal microbiota. However, these studies’ limited sources, small sample sizes, and varied study designs have led to inconsistent outcomes regarding glycaemic control. This study aimed to investigate the effects of symbiotics on the anthropometric measures, glycaemic control, and lipid profiles of patients with T2DM.MethodsA double-blind, placebo-controlled, parallel clinical trial was conducted at two diabetes outpatient clinics. The main researcher and participants were blinded to the capsule content throughout the study. Sixty-six patients with T2DM aged 30–75 years were randomly allocated, using even and odd numbers, into two equal groups. These groups received either symbiotic capsules containing 200 million colony-forming units plus fructo-oligosaccharide or a placebo for 12 weeks. The primary objective was a decrement in glycated haemoglobin [HbA1c]. The patients’ anthropometric measures, fasting blood sugar, high-density lipoprotein [HDL], low-density lipoprotein [LDL], total serum cholesterol and serum triglyceride levels were also assessed at baseline and after 12 weeks of intervention. Non-parametric tests were used for statistical analyses.ResultsWithin-group analysis revealed significant decreases in body mass index (BMI) and waist circumference (P = 0.005 and 0.023, respectively) and a significant increase in HDL levels in the symbiotic group (P = 0.04). HbA1c levels significantly increased in the placebo group (P = 0.016) but were not significantly reduced in the symbiotic group. The between-group analysis revealed significantly lower fasting blood sugar (FBS) levels in the symbiotic group, and higher in the placebo group (P = 0.02). No significant changes existed in total serum cholesterol, LDL, and triglyceride levels in either the symbiotic or placebo group.ConclusionsSymbiotics improve BMI, waist circumference, HDL, and FBS levels and prevent the worsening of HbA1c levels in patients with T2DM. Our preliminary results indicate the potential benefits of symbiotics in patients with T2DM, which may lead to better diabetes control. However, this evidence requires further assessment in larger trials.Trial registrationThe trial was registered retrospectively at the International Standard Registered Clinical/Social Study Number Registry (ISRCTN34652973) on 05/01/2024.

Objective To observe the levels of adiponection,leptin,blood fat and body mass index (BMI) after the probiotics intervention.Method We assessed the levels of adiponection,leptin,blood fat and BMI in 80 simple obesity children.They were divided into 2 groups.There were 20 boys and girls in each group.One group of children were given bifidobacterium,lactobacillus and streptococcus thermophilus 15 days.Results The levels of BMI and TG,TC,LDL-C of treatment group decreased.The levels of HDL-Cof treatment increased.But there were no significant difference between two groups (P ＞ 0.05).The levels of leptin (5.72 ± 0.39 vs 8.53 ±0.37) in treatment group decreased.The levels of adiponection (10.15 ±0.48 vs 8.81 ±0.37) Increased.There were significant difference between two groups (P ＜ 0.05).Conclusions Probiotics could decrease the levels of leptin and increase the levels of adiponection.Probiotics could become a new method to treat the simple obesity. Key words: Adiponection ; Leptin ; Probiotics ; Blood fat ; Simple obesity

Aim. To study the associations of angiotensinogen (AGT) (s4762(С521Т), rs699(Т704C)) and angiotensin II receptor type I (AGTR1) (rs5186(A1166C)) genetic polymorphisms with serum levels of insulin, glucagon, C-peptide, leptin, as well as with dyslipidemia and glycemic levels in Dagestan residents with combination of type 2 diabetes (T2D) and hypertension (HTN), as well as with isolated T2D/HTN.Material and methods. We examined 16 patients with isolated T2D, 59 patients with T2D+HTN and 51 patients with isolated HTN from Dagestan. Genetic polymorphisms of the AGT and AGTR1 genes were studied. The levels of insulin, glucagon, C-peptide, and leptin were studied by enzyme-linked immunosorbent assay (ELISA), while lipid and carbohydrate metabolism — by biochemical methods.Results. In patients with T2D, the association of CC genotype of AGT gene rs4762(С521Т) polymorphism with a leptin decrease was determined, while its CT genotype was associated with an increase in serum level of triglycerides. The TC genotype of AGT gene rs699(Т704C) polymorphism was associated with an increase in leptin, triglyceride and glucose levels. The AA genotype of AGTR1 gene rs5186(A1166C) polymorphism was associated with an increase in insulin and glucose levels, as well as a decrease in leptin level. In patients with a combination of T2D and HTN, CC and CT genotypes of AGT gene rs4762(С521Т) polymorphism was associated with a decrease in glucagon level. The TT genotype of AGT gene rs699(Т704C) polymorphism was associated with an increase in insulin, triglyceride, glucose and body mass index (BMI) levels. In isolated HTN, the CC and CT genotypes of AGT gene rs4762(С521Т) polymorphism were associated ith a decrease in glucagon level. The TT genotype of AGT gene rs699(Т704C) polymorphism was associated with increased levels of insulin, low density lipoproteins, and BMI.Conclusion. Associations of AGT (s4762(С521Т), rs699(Т704C)) and AGTR1 (rs5186(A1166C)) genetic polymorphisms with carbohydrate and lipid metabolism changes are an important pathogenetic link of T2D and HTN, which allows developing an individual prognosis of these diseases in Dagestan residents.