Management of Periodontal Intrabony Defect Using Sticky Bone and GTR Membrane: A Case Report

Background: Periodontal regeneration, a multifactorial and complex procedure relates to the reconstruction of the periodontal tissues to maintain a clinically healthful state. Attaining a complete periodontal regeneration with the current regenerative procedures offer a limited success. Platelet Rich Fibrin is a polymerized fibrin matrix having a simple strategy of enhancing healing capacity of natural blood clot by supplementing the natural blood clot with growth factors. This unique structure acts as a vehicle for growth factor delivery system and also carry cells that are essential for tissue regeneration. The potential limitations of PRF include cross-contamination with silica, a lack of rigidity, and fast degradation. To overcome these limitations, the third generation of platelet concentrate Titanium PRF was introduced by Tunali et al 2013 by using biocompatible material titanium, where the fibrin network has a longer absorption time, increased capacity for osseointegration, better hemocompatibility, greater cellular support, and it promotes periodontal regeneration. Aims and Objectives: The aim and objectives of this controlled clinical trial is to compare the clinical and Radiographic effectiveness of Leukocyte Rich Platelet rich fibrin [L-PRF] with open flap debridement, Titanium -Platelet Rich Fibrin[T-PRF] and Open Flap Debridement [OFD] in the management of 3 walled intrabony periodontal defects. Materials and Methods: This Randomized controlled clinical trial was conducted in the Department of Periodontics, Rural Dental College, Loni .25 patients with 3 walled intrabony defects at three sites were treated with OFD and L-PRF (experimental group), OFD and T-PRF (experimental group), OFD alone (control group). Evaluation of change in probing pocket depth, Gain in clinical attachment level was done at 9 Months, and defect fill and alveolar crestal bone resorption were evaluated at 6 and 9 Months after surgery by Analysis of Variance (ANOVA) with Post hoc Tukey’s test. Probing Pocket depth was measured from the gingival margin to the base of the pocket, Clinical attachment Level was measured from the cementoenamel junction of each tooth to the soft tissue base of the pocket by using a stent. Prior to surgery a standardized digital periapical radiograph was made using the customized bite-plate and the paralleling technique. Radiographic measurements was done as (1) distance from the Cemento Enamel Junction [CEJ] to the deepest point of the vertical bone defect (BD), (2) distance from the CEJ to the alveolar crest (AC). Measurements were obtained utilizing a millimetre grid. The differences between 6, 9 months and baseline values of CEJ-BD indicated the amount of bone fill. The differences between CEJ-AC will be identified as the amount of crestal bone resorption respectively. Results: The Change in Plaque Scores from Baseline to 9 Months at different time intervals depicted that the difference in mean PI Scores from Baseline to 1 Month, Baseline to 3 Months, Baseline to 6 Months and Baseline to 9 Months was statistically significant in all the patients. For the clinical parameters, the comparison of Change of PPD and gain in CAL (Baseline-9 Months) between OFD, L-PRF & T-PRF depicted that the mean change was highest for T-PRF, followed by L-PRF, followed by OFD. Radiographic Parameters which included defect fill demonstrated that for the T-PRF group the bone formation was more followed by L-PRF followed by OFD where bone formation was least at 6 and 9 Months. When the crestal bone resorption was assessed from baseline to 9 Months the mean distance in OFD Group was observed to be increased whereas in L-PRF there was no change and in T-PRF the mean distance from CEJ to AC was observed to be decreased (depicting bone formation only in T-PRF Group). Conclusion: With in the limitations of this study it can be concluded that the application of Titanium Platelet-rich fibrin presents new possibilities for enhanced healing and functional recovery in the treatment of IBDs with greater reduction in Pocket Depth, more Clinical Attachment gain and could produce more new bone in less time when compared to L-PRF and OFD.

The objective of the present study was to evaluate the clinical and radiographic outcomes of intrabony defects treated with decortication (intramarrow penetration) alone versus decortication combined with platelet-rich fibrin in periodontitis patients followed up for 6 months postsurgery. A total of 46 intrabony defects from periodontitis patients with a mean age of 36.30 ± 6.10 years were randomly assigned into two treatment groups. The control group (n = 23) intrabony sites were accessed with simplified papilla preservation flap (SPPF) followed with debridement, decortication, and closure. The test group (n = 23) sites were accessed with SPPF, followed with debridement, decortication, platelet-rich fibrin placement, and closure. The clinical parameters Plaque Index, Gingival Index, probing pocket depth, relative attachment level, gingival marginal level, along with radiographic defect depth and defect width were recorded at baseline, 3 months, and 6 months postsurgery. Gain in clinical attachment level was the primary outcome, and probing pocket depth reduction and radiographic bone fill were secondary outcomes of the study. The Plaque Index and Gingival Index scores showed nonsignificant difference on intra- and intergroup comparison at baseline, 3 months, and 6 months. The probing pocket depth was 8.17 ± 1.56 mm, 6.65 ± 1.30 mm, and 5.26 ± 1.18 mm for the control group, and 8.17 ± 2.01 mm, 6.26 ± 1.42 mm, and 4.78 ± 1.28 mm for the test group, at baseline, 3 months, and 6 months, respectively. The relative attachment level was 8.83 ± 1.40 mm, 6.78 ± 1.31 mm, and 5.39 ± 1.16 mm for the control group, and 8.39 ± 1.62 mm, 6.96 ± 1.36 mm, and 5.48 ± 1.20 mm for the test group at baseline, 3 months, and 6 months, respectively. Statistically significant reductions were observed for probing pocket depth for the control (2.91 mm, P < .001) and test groups (3.39 mm, P < .001), as well as for relative attachment level for the control (3.44 mm, P < .001) and test groups (2.91 mm, P < .001). However, intergroup differences were nonsignificant for probing pocket depth and relative attachment level. The radiographic defect depth was reduced by 0.31 mm for the control and 1.57 mm for the test group. The radiographic defect width was reduced by 0.18 mm for the control and 0.83 mm for the test group. Intergroup statistically significant differences were observed at the 6-month follow-up (P < .001) for radiographic defect depth and width. Within the limitations of the present study, the results demonstrate statistically significant intragroup improvements in clinical outcomes with decortication alone and decortication combined with platelet-rich fibrin in the treatment of intrabony defects in periodontitis patients. The addition of platelet-rich fibrin did not improve the clinical results beyond decortication alone, and unacceptable postsurgery residual pockets were observed in both the protocols. Considering the small sample size, the addition of platelet-rich fibrin resulted in significant bone fill over and above that of decortication alone.

Autologous platelet-rich fibrin (PRF) and barrier membranes in the treatment of intrabony defects in chronic periodontitis patients have shown significant clinical benefits. This study evaluates the additive effect of autologous PRF in combination with a barrier membrane versus the use of barrier membrane alone for the treatment of intrabony defects in chronic periodontitis patients. A randomized split-mouth design was used. Sixteen patients with 32 paired intrabony defects were included. In each patient 1 defect was treated using a resorbable collagen membrane along with PRF (test group) and the other defect by guided tissue regeneration alone (control group). The following clinical parameters were measured at baseline and after 9 months: plaque index, modified sulcus bleeding index, probing pocket depth, clinical attachment level, and gingival marginal level. The radiographic defect depth was also assessed at baseline and after 9 months. Test group showed a statistically significant improvement for probing depth (P = 0.002), clinical attachment level (P = 0.001), and radiographic defect depth (P < 0.001) after 9 months as compared with the control sites. Radiographic defect depth reduction was 58.19 ± 13.24% in the test group as compared with 24.86 ± 9.94% reduction in the control group. The adjunctive use of PRF in combination with barrier membrane is more effective in the treatment of intrabony defects in chronic periodontitis as compared with barrier membrane alone.

Platelet-rich plasma for periodontal regeneration in the treatment of intrabony defects: a meta-analysis on prospective clinical trials

Background and Objectives: Unfavorable intrabony defects (IBDs) are associated with the progression of periodontal disease and tooth loss. Growing scientific evidence has demonstrated the effectiveness of platelet concentrations in periodontal treatment. The aim of our study was to demonstrate the non-inferiority of an autogenous bone graft (ABG) associated with leukocyte- and platelet-rich fibrin (L-PRF) compared to ABG + Collagen Membrane in the treatment of IBDs. Material and Methods: Sixty-four patients with at least one IBD were randomly assigned to two groups: ABG+L-PRF and CM+ABG. Clinical and radiographic evaluations were performed at baseline and 12-month follow-up. Clinical attachment level (CAL), gingival recession (GR), probing pocket depth (PPD), and radiograph defect bone level (DBL) were compared between the two treatments. To evaluate the effectiveness of ABG+L-PRF, a non-inferiority margin of =1 mm (-1 mm for GR) was chosen; a second non-inferiority margin of =0.5 mm (-0.5 mm for GR) was set for clinical relevance. Results: At 12-month follow up, both treatments showed clinical and radiographic improvements. The 90% confidence intervals of the CM+ABG-L-PRF+ABG mean difference for CAL gain (-0.0564 mm [-0.316 to 0.203]), DBL gain (-0.433 mm [-0.721 to -0.145]), and PPD reduction (0.232 mm [0.015 to 0.449]) were below the 0.5 mm non-inferiority margin; the GR increase (0.255 mm [0.0645 to 0.445]) stayed above the -0.5 mm. Conclusions: the L-PRF+ABG treatment of unfavorable IBDs is non-inferior with respect to the CM+ABG therapy for CAL gain, but with a lower GR, a slightly higher PPD, and DBL gain.

Periodontitis is a chronic, multifactorial infectious disease affecting the oral cavity, destroying the periodontal supporting tissues, including alveolar bone resorption and loss of clinical attachment. These changes can result in the formation of intrabony defects and if left untreated, it can ultimately result in tooth loss. To enhance periodontal regeneration, several pharmacologic agents-alendronate (ALN), rosuvastatin (RSV), atorvastatin (ATV), metformin (MF), and melatonin (ML)-have demonstrated promising osteoanabolic and anti-inflammatory effects. When combined with platelet-rich fibrin (PRF), these agents may further enhance clinical and radiographic outcomes. This study aimed to assess the adjunctive benefits of various drug-PRF combinations on clinical attachment level (CAL) gain and bone fill (BF) in chronic periodontitis patients. This network meta-analysis (NMA) adhered to PRISMA-NMA standards and was recorded in the PROSPERO registry (CRD42024600432). An extensive literature search was conducted using the PubMed/ Medline, Wiley Online Library, Embase, and CENTRAL databases identified randomized controlled trials (RCTs) published after 2016. The analysis included ten studies comprising RCTs with 393 participants containing intrabony or furcation region defects and follow-up periods of 6 and 9months. PRF with MF demonstrated the highest efficacy for CAL gain (SUCRA 1.00), followed by PRF with ALN (0.60) and RSV (0.70). For bone fill, PRF with ALN ranked highest (SUCRA 0.90), followed by RSV (0.90) and MF (0.60). ML and ATV combinations showed modest effects, while PRF alone and placebo consistently ranked lowest (SUCRA ≤ 0.20). PRF combined with MF, ALN, and RSV appears to be the most effective therapeutic option for managing bony defects in chronic periodontitis. Future studies should confirm these findings through additional large and long-term clinical trials. The study provides clinically relevant evidence supporting the adjunctive use of pharmacologic agents with PRF to optimize regenerative outcomes. Such combinations can improve bone fill and attachment gain, offering predictable benefits for patients with chronic periodontitis.

BackgroundThe term periodontium encompasses the supporting structures around a tooth, including gingival tissue, alveolar bone, cementum, and periodontal ligament. Periodontal disorders are highly prevalent, affecting approximately 95% of the Indian population. Restoring the missing attachment apparatus is the main goal of regenerative therapy in cases of periodontal disease. The reproduction or rebuilding of damaged or lost periodontal tissue to restore the structure and functionality of the periodontium is known as regeneration. This study investigates the effectiveness of autologous sticky bone (ASB), autologous platelet-rich fibrin (PRF), and octacalcium phosphate–coated deproteinized bovine bone material (OCP-DBBM). These materials are being evaluated for their potential to enhance bone regeneration in infrabony defects, which are a significant concern in periodontal therapy. Effective bone regeneration is critical for the successful treatment of periodontal defects, as it can lead to improved clinical outcomes, including better attachment levels and reduced probing depths. This study aims to provide insights into the most effective methods for achieving these goals.ObjectiveWe aim to assess the efficacy of ASB and autologous PRF in conjunction with OCP-DBBM in infrabony defects at 6 months after surgery with regard to radiographic bone fill, reduction in probing pocket depth, and increase in clinical attachment level.MethodsThis randomized controlled clinical trial will be performed on 20 defects in patients diagnosed with stage 2 and 3 grade B periodontitis. It will be a parallel-designed study where group 1 (n=10) will be treated with ASB and group 2 (n=10) will be treated with autologous PRF and OCP-DBBM. One sitting will be required to perform the treatment, and a follow-up checkup will be done at 6 months.ResultsRecruitment procedures started in June 2025. All data are anticipated to be collected by February 2026. Full trial results are anticipated to be analyzed and submitted for publication by March 2026. The study’s anticipated end date is March 2026.ConclusionsBoth treatment approaches are expected to lead to notable gains in periodontal health and bone regeneration. The primary outcome, radiographic bone fill, and the secondary outcomes, clinical attachment level gain and probing pocket depth reduction, are critical indicators of treatment success.Trial RegistrationClinical Trials Registry-India CTRI/2024/06/069603; https://www.ctri.nic.in/Clinicaltrials/pmaindet2.php?EncHid=MTAzODk0&Enc=&userName=International Registered Report Identifier (IRRID)PRR1-10.2196/69666

Periodontitis is the most common condition, which causes bony defects; the ultimate goal of periodontal therapy is the regeneration of the destroyed tissues. There is always a need to search for better biomaterials that can be used for the treatment of intrabony defects. This study evaluated the effect of Moringa oleifera (MO) gel and platelet-rich fibrin (PRF) in the treatment of bone defects. We hypothesized that MO gel may increase the bone mineral contents and density of bone. The study was conducted on 16 defects in 8 adult male rabbits divided into 2 groups; group (1) buccal bone defect treated with moringa hydrogel and PRF (right site), group (2) buccal bone defect treated with PRF (left site). Computed tomography (CT) radiography and histological examination were assessed at baseline, 14 and 28 days. The defects were induced in the form of one osseous wall defect between the 1st and the 2nd molars. Comparisons between groups were done using an unpaired t-test. For comparison within each group, analysis of variance (ANOVA) was used. CT radiograph results showed there was a significant increase in bone density at 28 days in group 1 than in group 2 (843.13 ± 97.82 to 713.0 ± 51.09). The histological result revealed the defect area on the (PRF + Moringa) was almost filled completely by newly formed bone with few spots of retarded calcification. While (PRF) showed complete filling of the defect area by more fibrous tissue. The healing score showed a significant elevation of bone defect healing score in (PRF + Moringa group) when compared to (PRF group) at both times of evaluation. Radiographical examination, and histological and healing scores confirmed the superiority of Moringa + PRF results in an increase in bone fill and density in induced periodontal intrabony defects regeneration. Clinical trials should be considered to detect the effectiveness of MO in intrabony defects.

Introduction: Attempts to regenerate the periodontal osseous defect, which is lost as a result of periodontal disease, require the tapping of the innate healing potential of periodontium through appropriately designed therapeutic strategies. A multitude of grafted and non-grafted approaches have been used in the management of Intra-bony defects. However, they do not provide predictable periodontal regeneration. The aim of this study was to evaluate the combined effect of low-level laser therapy (LLLT) and platelet-rich fibrin (PRF), in site modulated intra-bony defects (decortication), which were accessed using a simplified papilla preservation flap (SPPF), on the clinical and radiographic outcomes of periodontal disease. Methods: A total of 30 patients with intra-bony defects were recruited for the study and randomly distributed in two groups (n=15). Test group sites were accessed with SPPF and the defects received intra-marrow Penetration (IMP) following debridement and were irradiated with a low-level laser followed by PRF grafting and suturing done. The control group defects were accessed with SPPF and grafted with PRF before being secured by sutures. The plaque and bleeding score, PPD, CAL, and the position of the gingival margin with radiographic defect depth were recorded and analyzed at baseline and six months post-intervention using the student's t test and Wilcoxon signed rank test. Results: The test group showed a clinically relevant increase in mean PPD reduction, CAL gain, and radiographic bone fill (3.6 ± 1.35 mm, 3.26 ± 1.16 mm and 2.44 ± 1.24 mm) compared to the control group (2.93 ±1.1 mm, 2.267 ± 1.33 mm and 1.26 ± 0.99 mm) six months post-intervention. However, intergroup comparison between the test and control groups did not show any statistically significant difference. Conclusion: These results highlights that test protocol had greater amelioration of the effects of periodontal disease and all the investigated clinical and radiographic parameters showed considerable improvement from baseline to 6 months within test and control group, but intergroup comparison between the test and control groups did not show any statistically significant difference, indicating statistical equivalence between the test and control protocol.

Background:Bone morphogenetic proteins have a powerful osteoinductive capacity and have been used as a new adjunct to graft materials for bone regeneration. The objectives of this systematic review are to assess the amount of radiographic bone fill, clinical attachment level (CAL) gain, and reduction in pocket depth (PD) in patients with intrabony defects in periodontitis patients following the use of recombinant human bone morphogenetic protein-2 (rhBMP-2).Materials and Methods:Electronic bibliographic databases search of Medline, Science Direct, and Google Scholar was made from January 1980 to December 2017. Studies using rhBMP-2 to treat periodontal intrabony defects of the maxillary or mandibular region with follow-up period of at least 6 months were searched. Two reviewers performed the systematic review using the PRISMA Statement for reporting and the Cochrane risk-of-bias tool was used for quality assessment.Results:It was found that rhBMP-2 showed statistically significant results with respect to radiographic defect resolution, CAL, and PD reduction at 9 months compared to open-flap debridement but showed statistically significant results only with respect to radiographic bone fill when compared with platelet-rich fibrin at 6 months.Conclusion:The rhBMP-2 may provide a promising alternative to traditional grafting procedures therapy that can enhance periodontal regeneration in patients having intrabony defects. Due to limited human studies, it can be concluded that no definitive evidence exists to ascertain the effectiveness of rhBMP-2 in the treatment of intrabony defects in periodontal diseases.

The three life stages that each have a unique impact on a woman's oral health are puberty, pregnancy, and menopause. The menopause affects women's periodontal and dental health. The aim of the study is to evaluate the effects of duration of menopause on periodontal clinical parameters such as clinical attachment loss (CAL), probing pocket depth (PPD), bleeding on probing (BOP), and plaque index (PI) in menopause women. 74 menopausal women between 45 to 70 years were participated. The patient was informed about the study protocol and consent was taken. Information about the onset of menopause, periodontal clinical parameters such as CAL, PPD, BOP, PI and demographic data were recorded in the specialized case history Performa. The most commonly lost tooth was the mandibular first molar. A significant positive correlation was found between age and tooth loss (p = 0.010). While age did not significantly correlate with CAL, PPD, BOP, or PI. The duration of menopause showed significant positive correlations with all parameters: PPD (p = 0.003), CAL (p = 0.009), BOP (p = 0.013) and PI ( p = 0.010). The primary causes of tooth loss were grossly decayed teeth (33.7%), caries (27.0%), and periodontal disease (20.2%). This study found that the duration of the menopause is positively correlated with CAL, PPD, BOP, and PI, and that age is significantly associated with the number of tooth loss in menopausal women. In order to address periodontal and dental health issues, these results emphasise the significance of customised oral health interventions for menopausal women.

Background and Objectives:The primary goal of periodontal therapy is to restore the tooth supporting tissues lost due to periodontal disease. The aim of the present study was to compare the efficacy of combination of type I collagen (GTR membrane) and xenogenic bone graft with open flap debridement (OFD) in treatment of periodontal intrabony defects.Materials and Methods:Twenty paired intrabony defects were surgically treated using split mouth design. The defects were randomly assigned to treatment with OFD + collagen membrane + bone graft (Test) or OFD alone (Control). The clinical efficacy of two treatment modalities was evaluated at 9 month postoperatively by clinical, radiographical, and intrasurgical (re-entry) parameters. The measurements included probing pocket depth (PD), clinical attachment level (CAL), gingival recession (GR), bone fill (BF), bone density (BD) and intra bony component (INTRA).Results:The mean reduction in PD at 0–9 month was 3.3±0.82 mm and CAL gain of 3.40±1.51 mm occurred in the collagen membrane + bone graft (Test) group; corresponding values for OFD (Control) were 2.20±0.63 mm and 1.90±0.57 mm. Similar pattern of improvement was observed when radiographical and intra-surgical (re-entry) post operative evaluation was made. All improvement in different parameters was statistically significant (P< 0.01).Interpretation and Conclusion:Treatment with a combination of collagen membrane and bone graft led to a significantly more favorable clinical outcome in intrabony defects as compared to OFD alone.

Platelet-Rich Fibrin (PRF) and bioactive glass putty have been shown to be effective in promoting reduction in probing depth, gain in clinical attachment, and defect fill in intrabony periodontal defects. The individual role played by bioactive glass putty in combination with PRF is yet to be elucidated. To compare the clinical effectiveness of the combination of PRF and bioactive glass putty and bioactive glass putty alone as regenerative techniques for intrabony defects in humans. Ten pairs of intrabony defects were surgically treated with PRF and bioactive glass putty (Test group) on one side or bioactive glass putty alone (Control group) on other side. The primary outcomes of the study included changes in probing depth; attachment level and bone fill of osseous defect. The clinical parameters were recorded at baseline, 3, 6, and 9 months. Radiographic assessment was done using standardized intraoral periapical radiographs. Differences between baseline and postoperative measurementsbetween the control and test groups were calculated using independent t-test. Comparisons were made within each group between baseline, 3 months, 6 months and 9 months using the ANOVA test followed by Bonferroni test. The mean probing depth reduction was greater in the test group (bioactive glass putty and PRF) i.e., (3.2±2.3 mm) than in the control group (bioactive glass putty alone) i.e., (3.15±1.06 mm). The mean CAL gain was also greater in the test group (4.1±1.73 mm) as compared to the control group (3.15±1.06 mm), (p-value<0.95). Furthermore significantly greater mean bone fill was found in the test group (7.1±1.37 mm) as compared to the control group (5.7 ± 1.64 mm), (p-value<0.043). The results of this study showed both the groups bioactive glass putty alone (Control Group) and the combination of PRF and bioactive glass putty (Test Group) are effective in the treatment of intrabony defects. The bioactive glass putty appears to be a suitable vehicle to administer biologic substances like PRF and growth factors to induce the new bone regeneration.

Objective: This study aimed to evaluate the efficacy of bovine pericardium as a barrier membrane for guided periodontal tissue regeneration.Material and Methods: The records of 20 periodontitis patients with a median age of 44.50 (IQR17) who underwent guided tissue regeneration (GTR) using bovine pericardium membranes (PericardLEMB, Tissue Bank, USM, Malaysia) were retrieved. The parameters, including plaque score (PS), gingivitis score (GS), probing pocket depth (PPD), clinical attachment loss (CAL), and alveolar bone loss from pre- to post-6-months of GTR, were compared using the Wilcoxon Paired Signed-Ranks test.Results: We found statistically significant improvements in all of the parameters 6 months post-GTR (p-value&lt;0.05). The median and interquartile range (IQR) for PS and GS decreased from 20.68% (IQR18.60) to 12.62% (IQR11.10) and 25.90% (IQR25.58) to 10% (IQR17.73), respectively. Similarly, after 6 months, median PPD and CAL had decreased to 3.25mm (IQR1.56) and 3.25mm (IQR1.92), respectively, from the baseline values of 7.00mm (IQR3.38) and 7.50mm (IQR3.61). Consistently, significant radiographic alveolar bone gain (20%) was observed 6 months after GTR.Conclusion: Despite this study’s limitations, such as small sample size, lack of control group, and short follow-up duration, it is conceivable to propose that utilizing BPM as bioabsorbable barrier membranes in the GTR procedure promotes periodontal tissue regeneration.

Introduction: Intrabony periodontal defects respond well to regenerative periodontal therapy. Numerous grafts and non graft materials are available for regeneration. Careful use of nonallogenic bone graft could enhance radiographic defect fill. Aim: To compare the clinical and radiographical evaluation of bovine derived xenograft (OsseograftTM) alone versus a combination of bovine derived xenograft and calcium sulphate hemihydrate (OsseomoldTM) in the treatment of intrabony defects in chronic periodontitis. Materials and Methods: A prospective, single blinded randomised clinical trial was conducted in the department of Periodontics, Nair Hospital Dental College, Mumbai, India (December 2017- August 2019). A total of 42 patients presenting with 43 intrabony defects were randomly assigned to Control Group (CG) (n=21) or Test Group (TG) (n=22). Clinical parameters {Probing Pocket Depth (PPD) and Clinical Attachment Level (CAL)} were assessed at baseline (M0), one month (M1), three months (M3) and six months (M6) and radiographic parameters {Bone Fill (BF)} were measured using Intraoral Periapical Radiograph (IOPA) at baseline (M0) and six months (M6). Two patients (three defects) were lost to follow-up. Descriptive and inferential statistical analyses were performed, results on continuous measurements were presented on Mean±SD. Statistical software IBM SPSS statistics 20.0. Level of significance was fixed at p=0.05. Student’s t-test was used to find the significant difference between and within the groups. Repeated measures Analysis of variance (ANOVA) was used to find the significance of study parameters within the group (at different time intervals). Results: PPD was lowest at six months for TG (3.95±0.61) and CG (3.30±0.66) and it gradually improved from baseline to six- months (p&lt;0.001 for both TG and CG). CAL gain was highest at six-months for TG (4.4±0.50) and CG (3.65±0.75) (p&lt;0.001). Significant reduction in Radiographic Defect Depth (RDD) was noted in both the groups (CG: 6.65±1.08 at M0 and 4.92±1.00 at M6 (p&lt;0.001); TG: 7.06±0.96 at M0 and 5.14±0.77 at M6) (p&lt;0.001). Intergroup analysis was statistically significant for clinical parameters with greater improvement seen in CG control group {PPD and CAL at M3 and M6 (p&lt;0.001)} and statistically insignificant for radiographic parameters (p&gt;0.5). BF was higher at M6 in TG (1.87) as compared to CG (1.72), which was statistically insignificant. Conclusion: Both treatments were clinically effective showing a significant improvement in clinical and radiographic parameters and there was significant difference between the two groups- clinically in terms of reduction in PPD and CAL gain at three months and six months with greater improvement seen in CG as compared to TG, with no difference radiographically. Further studies are needed to show the stability over time of the present results.