SLAM is one of the most critical technologies for mobile robots. Vision-based SLAM techniques have recently become popular. In this study, a high-density reconstruction technique based on monocular camera, MDF-SLMA, is proposed to solve the constraints of stereo and RGB-D cameras. The DSO-based tracking thread is used for RGB input images. To estimate the depth of key frames, an updated deep learning technique is used. Attitude optimization and density mapping are based on the results of depth estimation. Experimental results show that the proposed method can achieve monocular density mapping and reduce SLAM trajectory error. MDF-SLAM lays the foundation for mobile robots to detect their surroundings and perform complex tasks.