Methods for Characterization of Surface Charge and Solid–Liquid Interaction Studies of Biomaterials

Ultrasensitive detection of nucleic acids and proteins using quartz crystal microbalance and surface plasmon resonance sensors based on target-triggering multiple signal amplification strategy

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTQuartz Crystal Microbalance and Surface Plasmon Resonance Study of Surfactant Adsorption onto Gold and Chromium Oxide SurfacesFrank Caruso, Takeshi Serizawa, D. Neil Furlong, and Yoshio OkahataCite this: Langmuir 1995, 11, 5, 1546–1552Publication Date (Print):May 1, 1995Publication History Published online1 May 2002Published inissue 1 May 1995https://pubs.acs.org/doi/10.1021/la00005a023https://doi.org/10.1021/la00005a023research-articleACS PublicationsRequest reuse permissionsArticle Views1194Altmetric-Citations112LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts

Liposome and proteoliposome fusion onto solid substrates, studied using atomic force microscopy, quartz crystal microbalance and surface plasmon resonance. Biological activities of incorporated components

Volatile emissions can be efficiently reduced by membrane separation processes. Selecting the most adequate membrane polymer can be a time and resource‐intensive screening process. It is demonstrated that surface‐sensitive techniques such as the quartz crystal microbalance (QCM) and surface plasmon resonance (SPR) can be valuable tools to significantly cut down on the time needed to characterize and quantify vapor‐polymer interactions. Both techniques are shown to be highly complementary. QCM allows also obtaining qualitative data on changes in the polymer viscoelasticity upon vapor sorption which ultimately might permit a correlation with the mechanical membrane stability.

Biotinylated Steroid Derivatives as Ligands for Biospecific Interaction Analysis with Monoclonal Antibodies Using Immunosensor Devices

Summary:Monotype functionalizations with different types of functional groups (OH, NH2, COOH) on polypropylene and poly(tetrafluoroethylene) surfaces were synthesized using pulsed plasma‐initiated homo‐ or copolymerization of functional group‐carrying monomers. The maximum concentrations of functional groups were 31 OH, 18 NH2or 24 COOH groups per 100 C atoms using allyl alcohol, allylamine or acrylic acid respectively as the monomer. The measured peel strengths of aluminium deposits increased linearly with the concentration of functional groups. Near the maximum concentration of OH (>27 OH/100 C atoms) or at moderate concentrations of COOH groups (>10 COOH/100 C atoms), constant (maximum) peel strengths were measured due to the mechanical collapse of one component in the composite (cohesive failure). Interface failures in Al‐PP composites were found with COOH, NH2and OH groups and cohesive failures were seen when higher concentrations of COOH groups were applied (>10 COOH/100 C atoms).Dependence of Al peel strength in Al‐plasma polymer‐PP systems on the type and density of functional groups in plasma polymer interlayers.imageDependence of Al peel strength in Al‐plasma polymer‐PP systems on the type and density of functional groups in plasma polymer interlayers.

Comparison of surface plasmon resonance spectroscopy and quartz crystal microbalance techniques for studying DNA assembly and hybridization

We demonstrate that a new, stable, artificial TATA (T — thymine, A — adenine) box is recognized by amino acids recognizing the natural TATA box. Here, the former mimicked, as a minimal motif, oligodeoxyribonucleotide interactions with amino acids of proteins involved in repairing of damaged dsDNA. By electropolymerization, we molecularly imprinted non-labeled 5′-TATAAA-3′ via Watson-Crick nucleobase pairing, thus synthesizing, in a one-step procedure, the hexakis[bis(2,2′-bithien-5-yl)] TTTATA and simultaneously hybridizing it with the 5′-TATAAA-3′ template. That is, a stable dsDNA analog having a controlled sequence of nucleobases was formed in the molecularly imprinted polymer (MIP). The 5′-TATAAA-3′ was by the X-ray photoelectron spectroscopy (XPS) depth profiling found to be homogeneously distributed both in the bulk of the MIP film and on its surface. The 5′-TATAAA-3′ concentration in the 2.8(±0.2)-nm relative surface area, ~140-nm thick MIP film was 2.1 mM. The MIP served as a matrix of an artificial TATA box with the TATAAA-promoter sequence. We comprehensively characterized this artificial DNA hybrid by the polarization-modulation infrared reflection-absorption spectroscopy (PM-IRRAS) and X-ray photoelectron spectroscopy (XPS). Further, we examined interactions of DNA repairing TATA binding protein (TBP) amino acids with the artificial TATA box prepared. That is, molecules of l-phenylalanine aromatic amino acid were presumably engaged in stacking interactions with nucleobase steps of this artificial TATA box. The nitrogen-to‑phosphorus atomic % ratio on the surface of the MIP-(5′-TATAAA-3′) film increased by ~1.6 times after film immersing in the l-glutamic acid solution, as determined using the XPS depth profiling. Furthermore, l-lysine and l-serine preferentially interacted with the phosphate moiety of 5′-TATAAA-3′. We monitored amino acids interactions with the artificial TATA box using real-time piezoelectric microgravimetry at a quartz crystal microbalance (QCM) and surface plasmon resonance (SPR) spectroscopy under flow injection analysis (FIA) conditions.

Quartz crystal microbalance (QCM) and surface plasmon resonance (SPR) were used to study zipper and layer-by-layer multilayer assemblies of artificial photosystems based on naphthalenediimides (NDIs) attached to an oligophenylethynyl (OPE-NDI) or p-oligophenyl (POP-NDI) backbone in dry and wet state. For the most interesting OPE-NDI zipper, one obtains for the dry film a monolayer thickness of 1.85 nm and a density of 1.58 g/cm(3), while the wet film has a larger monolayer thickness of 3.6 nm with a water content of 36%. The dry thickness of a monolayer in OPE-NDI zippers corresponds to about one-half of the length of the OPE scaffold in agreement with the proposed structure of the zipper. The low water content of the OPE-NDI films confirms their compact structure. The dry monolayer thickness of the POP-NDI films of 1.45 nm is smaller than that for the OPE-NDI films, which is probably related to a tilt of the POP scaffolds within the adsorbed layer. The POP-NDI films swell in water much more substantially, suggesting a much more open structure. These features are in excellent agreement with the better photophysical performance of the OPE-NDI assemblies when compared to the POP-NDI films.

Mechanism of lysozyme adsorption onto gold surface determined by quartz crystal microbalance and surface plasmon resonance

Gold nanohybrid systems with tunable fluorescent feature: Interaction of cysteine and cysteine-containing peptides with gold in two- and three-dimensional systems

Perchlorate removal by activated carbon adsorption

4-Aminophenyl boronic acid modified gold platforms for influenza diagnosis

The object of the present investigations was to study the effect of the adsorption of charged organic ions on electrically charged, solid-liquid interfaces. To that end, symmetrical quater nary ammonium ions were adsorbed on a silver iodide-electrolyte interface at various surface charges. The electrochemistry of this model interface is well developed in comparison with other solid liquid interfaces and it is very suited for fundamental electrosorption studies. Special attention was paid to some general prob lems in the field of electrosorption:- The effect of solvent- and other dipoles on interfacial properties.- The presence of a maximum as a function of the surface poten tial in the adsorption of organic compounds.- The applicability of equilibrium interfacial thermodynamics.- The discrepancy between the specific surface areas obtained according to different methods.It appears that the study of the adsorption of tetraalkylammonium (TAA) ions contributes to the solution of some of these general problems.Electrosorption of TAA +ions has been investigated in 0.1 M KNO 3 in order to maintain a constant ionic strength and to suppress diffuse double layer effects. The electrical properties are ob tained from experimental charge-potential curves and from measure ments of the electrophoretic mobility. Adsorption of TAA +ions at various valves of the surface potential has been measured analytically. This thesis can be subdivided into three more or less separate parts: the theoretical chapters 2 and 3, the automation chapter 4, and the experimental chapters 5, 6 and 7 on the electrosorption of TAA +ions.Interfacial thermodynamics has been elaborated for a system containing strongly adsorbing organic ions. Ionic components have been introduced into Gibbs' adsorption equation, and the solidliquid boundary was taken as Gibbs' dividing plane. interfacial excess quantities were related to the temperature, concentrations of the potential determining ions, concentration of the organic compound, and the ionic strength. A discussion has been given on the definition of the surface charge. It was shown that a consistent thermodynamical definition of the surface charge eliminates non-thermodynamic terms in the equations for the temperature dependency of the surface charge. The adsorption isotherm for a binary solution was derived from a quasi- thermodynamic approach. It was discussed how electrical effects can be introduced in the adsorption isotherm. The occurrence of congruency in the potential or the charge facilitates the evaluation of the adsorption Gibbs energy.A new approach of the contribution of dipolar - or Χpotentials to the properties of a solid-electrolyte interface in the absence of an organic adsorbate has been described. It was assumed that dipolar potentials at the solid-liquid boundary arise as a consequence of local properties of the interface (hydration) and not from the action of a homogeneous electrical field. The hydration of solid particles is thus treated as a patchwise phenomenon, in which the orientation of water dipoles varies in a patchwise fashion along the surface. The idea is applied to the AgI system, where besides the neutral surface, Ag +patches and I -patches are distinguished. A first-order elaboration is offered including an analysis of the relation between overall macroscopic quantities and local properties. It was assumed that the large difference in hydration free energies of Ag +and I -leads to a difference in the contribution of the adsorbed Ag +and I -to the dipolar potential across the interface. The model offers an interpretation of two characteristics of double layers on silver iodide: the asymmetry of its p.z.c. and the conspicuous capacitance rise on the positive side, and it may serve as a starting point for a better description of the typical differences between the double layers on AgI and Hg.The surface charge of a silver iodide suspension can be determined as a function of the surface potential (or pAg) by potentiometric titration with Ag +and I -ions. This determination is very important, because the charge-potential relation can be considered as a "finger- print" in electrosorption studies. However, the manual procedure is very time-consuming. Therefore, the procedure has been automatized with a PDP11-computer and a CAMAC data acquisition system. This laboratory automation system enables the measurement of one complete titration curve independent of human observation. The reliability of the system appears to be excellent.The major part of the experimental study was concerned with the electrosorption of tetrapropyl-, tetrabutyl-, and tetraamylammonium ions on Agl. The results enabled the development of a selfconsistent picture of the adsorption mechanism.Charge-potential curves in the presence of TAA +ions are rather similar to those obtained for e.g. n-butanol, except that the curves tend to merge at the positive side, implying complete desorption of TAA +at increasing positive potentials. The most noteworthy common feature is the occurrence of a more or less welldefined common intersection point. According to interfacial thermodynamics this point corresponds to an adsorption maximum as a function of the surface potential. This result may appear surprising. From the coulombic interaction between a positive ion and an increasingly negative surface we would expect an increase in affinity The observation that, after surpassing a certain surface potential, the affinity decreases is an indication that the dipolar contribution due to the energy of displacement of water molecules is able to overcompensate even the coulombic interaction.The desorption at increasing positive potentials enables a quantitative test of Gibbs' law by comparing calculated adsorption values with those measured analytically. It has been shown that both for tetrapropyl- and tetrabutylammonium. ions the agreement be tween calculated and direct values is within about 20%. This is probably within experimental error. This basically is a very important result. because it is the first quantitative verification of the applicability of Gibbs' law to the AgI system. It proves that adsorption of both potential determining ions and tetraalkylammonium ions is reversible and obeys the thermodynamic laws.Tetraalkylammonium nitrates are strong electrolytes. The presence of an adsorption maximum could suggest that TAA +ions are (mainly) adsorbed as neutral entities, as was suggested for tetraalkylammonium halides on mercury. However, from measurements of the electrophoretic mobility of silver iodide sols it follows, that the major part of the ions are adsorbed as non-paired ions.It appears that the adsorption Gibbs energy of tetraalkylammonium ions consists of three contributions of an approximately comparable order. The dipolar and the coulombic contributions were already mentioned above. Hydrophobic bonding is the third contribution, the operationality of which can be inferred from comparison of the adsorption of the homologous series of TAA +ions and the effect of an increasing number of CH 2 groups.Analysis of the adsorption data shows that the isotherms are neither perfectly congruent in the surface charge nor in the surface potential. However, from interpretation according to the Frumkin, Fowler- Guggenheim isotherm equation congruency in the charge seems more probable because only in that case a positive interaction parameter is found. Congruency in charge implies that the field strength at the adsorption site is more relevant than the potential.Significant progress has been made in the interpretation of the presence and the position of the adsorption maximum (or common intersection point). An equation for the position of the common intersection point has been derived both for the case of congruency in charge and in potential taking into account the effect of the adsorption of an organic ion on the various contributions to the total Galvani potential. According to the equation derived, the shift of the common intersection point with increasing length of the alkyl chains of the TAA +ions is correctly accounted for if the thickness of the Stern layer and the radius of the tetraalkylammonium ion are identical.Several properties of the silver iodide-electrolyte interface were compared with the mercury interface in the presence of adsorbed n-butanol, tetrapropylammonium and tetrabutylammonium ions. It appears that the potential of the common intersection point is about the same for both systems with the three adsorbates, whereas the charge of that point is a factor of 2 higher for mercury. The monolayer coverage for TBuA +is about a factor of 3 higher for mercury. These results are not completely understood but may be related to the difference in the natures of the surface charges for both systems or to the problems in choosing a proper specific surface area for silver iodide.The specific surface area as determined with the adsorption of tetraalkylammonium ions or dyes differs by about a factor of 3 to 3.5 from the area obtained by comparing double layer capacitances of AgI and Hg at low salt concentrations. The difference must either be due to a failure in the comparison of double layer capacitances of both surfaces or to a different behaviour in the adsorption of the organic adsorbate. As by negative adsorption also the capacitance area is obtained, the latter factor was chosen. An adsorption model was introduced to explain the difference in monolayer coverage between AgI and Hg in terms of differences in lateral mobility of the adsorbate.Resuming, it can be concluded that the electrosorption of tetraalkylammonium ions on silver iodide has given new insight into some general and specific interfacial properties. Significant progress has been made in the experimental verification of Gibbs' law, in the description of the adsorption maximum, and in the behaviour of water dipoles on the surface.

Detection and binding interactions of pharmaceutical contaminants using quartz crystal microbalance – Role of adsorbate structure and surface functional group on adsorption