Network Modelling on Tropical Diseases vs. Climate Change

Iinsulin Levels and Homeostasis Model Assessment of Insulin Resistance (HOMA-IR) May Explain a Possible Important Role of the Pancreas In Sickle Cell Disease Patients Outcome

Effects of FSH extracted from in vitro cultured anterior pituitary cells of male buffalo calves on body and testes weight, serum FSH and total cholesterol and hematological variables in male rabbits

Summary: The haematological effects following ingestion of shellfish exposed to crude oil polluted water or the pollutant perse were investigated in albino Wistar rats. Feeding of four groups of rats for 28 days duration with two reference casein or shellfish protein control diets (Group A and B), and two test diets (Group C and D) supplemented at varying levels with shellfish which had been previously exposed to crude oil polluted water and the oral gavaging with crude oil at the rate of 3, 6 and 9 ml/kg body weight per day to three groups (groups II, III and IV respectively) of rats for 7 days duration resulted in changes in packed cell volume (PCV), red blood cell (RBC) and white blood cell (WBC) counts, and haemoglobin concentration (Hb) of rats. Group C and D respectively fed 10% and 20% polluted shellfish diets recorded significant (P < 0.05) decreases in PCV and RBC counts while Hb concentration and WBC counts increased. Groups II, III and IV gavaged with varying doses of crude oil recorded significant (P < 0.05 – 0.01) dose dependent decrease in PCV and RBC counts when compared to controls (group 1). Hb and WBC counts also increased for these groups but the increase was only significant for WBC counts (P < 0.05) when compared with controls. The results suggest that the ingestion of shellfish exposed to crude oil polluted water or the polluted perse results in haematotoxicity.

Diagnosis of Periprosthetic Joint Infection

Hyperuricemia is a common metabolic disorder linked to various health conditions. Its prevalence varies among populations and genders, and high-altitude environments may contribute to its development. Understanding the connection between blood cell parameters and hyperuricemia in high-altitude areas can shed light on the underlying mechanisms. This study aimed to investigate the relationship between blood cell parameters and hyperuricemia in high-altitude areas, with a particular focus on gender differences. We consecutively enrolled all eligible Tibetan participants aged 18-60 who were undergoing routine medical examinations at the People's Hospital of Chaya County between January and December 2022. During this period, demographic and laboratory data were collected to investigate the risk factors associated with hyperuricemia. Among the participants, 46.09% were diagnosed with hyperuricemia. In the male cohort, significant correlations were found between serum uric acid (SUA) levels and red blood cell (RBC) count, creatinine (Cr). Urea, alanine transaminase (ALT), and albumin (ALB). Notably, RBC exhibited the strongest association. Conversely, in the female cohort, elevated SUA levels were associated with factors such as white blood cell (WBC) count. Urea, ALT, and ALB, with WBC demonstrating the most significant association. Further analysis within the female group revealed a compelling relationship between SUA levels and specific white blood cell subtypes, particularly neutrophils (Neu). This study revealed gender-specific associations between SUA levels and blood cell parameters in high-altitude areas. In males, RBC count may play a role in hyperuricemia, while in females, WBC count appears to be a significant factor. These findings contribute to our understanding of metabolic dynamics in high-altitude regions but require further research for comprehensive mechanistic insights.

Background: Severe acute malnutrition (SAM) remains a critical public health issue, particularly in resource-limited settings, significantly impacting child morbidity and mortality. Malnutrition adversely affects hematopoiesis, leading to anemia, leukocyte abnormalities, and thrombocytopenia, which further compromise immune function and increase susceptibility to infections. Understanding the hematological alterations in children with SAM is essential for early detection, appropriate management, and improved clinical outcomes. This study aimed to assess the hematological profile of children with SAM and identify specific abnormalities associated with the condition. Objective: To evaluate the hematological parameters of children with severe acute malnutrition and compare them with healthy controls. Methods: This descriptive cross-sectional study was conducted over nine months, from January to September 2023, at the Nutrition Unit of PEDS Shaikh Zayed Hospital, Larkana. A total of 216 children aged 7 months to 5 years were included, with 108 diagnosed with SAM based on WHO criteria and 108 healthy controls. Hematological parameters, including red blood cell (RBC) count, hemoglobin (Hb), hematocrit (HCT), mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), mean corpuscular hemoglobin concentration (MCHC), white blood cell (WBC) count, and platelet count, were analyzed using an automated hematology analyzer (XN-350). Socioeconomic and demographic data were collected through structured questionnaires. Results: Among the 108 children with SAM, 56 (51.8%) were male, and 52 (48.1%) were female. The majority, 68 (49.2%), were aged 7–24 months. Rural residency was reported in 67 (62%) cases, and 74 (68.5%) belonged to low-income families. Normochromic normocytic anemia was the most prevalent type, affecting 42 (38.88%) children, followed by iron deficiency anemia in 28 (25.9%) and megaloblastic anemia in 2 (1.85%). Hemoglobin levels were significantly lower in SAM cases (8.13±1.09 g/dL) than in controls (9.80±1.21 g/dL) (p=0.0005). Hematocrit was also reduced in cases (25.07±3.34%) compared to controls (30.88±4.02%) (p=0.0005). RBC count was lower in cases (3.99±0.71 ×10⁶/µL) than controls (4.10±0.51 ×10⁶/µL) (p=0.0003). MCV, MCH, and MCHC were all significantly reduced in cases (60.45±4.93 fL, 18.75±2.15 pg, and 30.11±1.74 g/dL, respectively) compared to controls (70.35±6.14 fL, 24.39±2.99 pg, and 32.94±1.72 g/dL, respectively) (p&lt;0.05). WBC count was lower in the SAM group (6.99±1.64 ×10³/µL) compared to controls (8.24±4.28 ×10³/µL) (p=0.005), and platelet count was also significantly reduced (262.36±85.22 ×10³/µL vs. 302.11±128.15 ×10³/µL) (p=0.002). Conclusion: Children with severe acute malnutrition exhibited significant hematological abnormalities, including anemia, leukocyte alterations, and thrombocytopenia. The high prevalence of normochromic normocytic anemia and iron deficiency anemia underscores the profound impact of malnutrition on hematopoiesis. These findings emphasize the need for early identification, targeted nutritional interventions, and routine hematological monitoring to improve health outcomes in malnourished children.

Comparative pharmacokinetics and a clinical laboratory evaluation of intravenous acetaminophen in Beagle and Galgo Español dogs

Sulphamethoxazole/trimethoprim (SMX/T) is an antimicrobial agent that has been used for decades against both Gram-positive and Gram-negative bacteria as well as protozoa. It is particularly used as prophylaxis against Pneumocystis carinii pneumonia (PCP) in patients with acquired immune-deficiency syndrome (AIDS). Its use in dogs with associated adverse reactions has necessitated an experimental study that may enhance clinical management of the toxicities. The study aimed to determine the effects of prolonged treatment with high doses of sulphamethoxazole/trimethoprim on the haematological profile of dogs. Dogs were given SMX/T at 30, 60 and 120 mg/kg body weight at 12-h intervals for 21 days. The packed cell volume (PCV), haemoglobin concentration (HbC), red blood cell (RBC) count and total and differential white blood cell (WBC) counts were performed using standard techniques. Data generated was analysed by one-way analysis of variance (ANOVA). Alopecia, epistaxis and skin eruptions were seen at the dose of 120 mg/kg. There were also dose-dependent decreases in the PCV and RBC count. HbC was significantly (p < 0.05) lower than the control only at the dose of 120 mg/kg. Total WBC count was significantly (p < 0.05) lower than the control only on day 7 of treatment at 30, 60 and 120 mg/kg. Beyond day 7, the white cell response, total and differential WBC counts, were fluctuating with significant depression at the dose of 120 mg/kg. Increase in dose and prolonged use of SMX/T in the dogs decreased the haematological parameters and increased the occurrence of toxic reactions and may outweigh the benefits of treatment with the drug in recommended cases.

Background: Cerebrospinal fluid (CSF) analysis is essential for diagnosing various disorders affecting the central nervous system (CNS). Traditionally, CSF cell count analysis is performed manually using a Neubauer chamber hemocytometer, which is labor-intensive and prone to subjective interpretation. Methods: In this study, we evaluated the analytical and clinical performance of the Microscanner C3, an automated cell counting system, for CSF analysis using artificially prepared samples and 150 clinical CSF samples. Results: The lowest detectable white blood cell (WBC) count was 3.33 cells/µL, and the lowest detectable red blood cell (RBC) count was 3.67 cells/µL. The coefficients of variation (CV%) for the Microscanner C3 were lower than those for the Neubauer chamber at all cell concentrations. The correlation coefficients (R) between the Microscanner C3 and conventional methods were high: 0.9377 for WBCs and 0.9952 for RBCs when compared with the Neubauer chamber, and 0.8782 for WBCs and 0.9759 for RBCs when compared with the flow cytometer. Additionally, the Microscanner C3 showed good agreement with both the Neubauer chamber and flow cytometer in the Passing–Bablok regression analysis and Bland–Altman analysis for WBC count at all concentrations and RBC count at concentrations of 0–1000 cells/µL. Conclusions: The Microscanner C3 proved to be more sensitive, precise, and consistent compared to the conventional hemocytometer. The new system is also compact, convenient, and cost-effective, making it a valuable option for clinical laboratories.

Objective Lusutrombopag is a thrombopoietin receptor agonist approved to treat thrombocytopenia in patients with chronic liver disease (CLD). This post hoc analysis of the Japanese L-PLUS 1 and global L-PLUS 2 trials aimed to clarify factors related to platelet count increase after lusutrombopag treatment. Methods In L-PLUS 1, Pearson’s correlation coefficients were used to evaluate correlations between platelet count and spleen index, thrombopoietin concentration, white blood cell (WBC) counts, and red blood cell counts (intent-to-treat [ITT] population). Associations between platelet count increase after lusutrombopag treatment and each parameter were assessed by regression analysis and mixed-effect model for repeated measures (MMRM). Associations between time-dependent changes in platelet count increase and each parameter were also examined in the L-PLUS 2 lusutrombopag ITT population by MMRM. Results In L-PLUS 1, the baseline platelet count was correlated with pretreatment spleen index (r = −0.23, 95% confidence interval [CI] −0.41 to −0.03) and WBC count (r = 0.26, 95% CI 0.06 to 0.43). No selected parameters were associated with the maximum platelet count increase from baseline. Patients with WBC counts below the normal range showed smaller platelet count increases after lusutrombopag treatment than patients with WBC counts within the normal range (p = .0028). In L-PLUS 2 (p = .0533), findings were similar and confirmed by larger pooled data of L-PLUS 1/L-PLUS 2 (p = .0021). Conclusions This post hoc analysis revealed a possible association between baseline WBC count and platelet count increase after lusutrombopag treatment. WBC count could be a relevant factor for lusutrombopag efficacy.

To the Editor: Li et al1 evaluated workplace exposures to 1-bromopropane (CASRN 106-94-5) at three separate manufacturing facilities in China (ie, Yixing, Yancheng, and Weifang).1 The authors identified a number of statistically significant changes in various endpoints evaluated in female and male workers. Li et al1 reported that women were more susceptible than men and identified a lowest adverse effect level (LAEL) of 1.28 ppm in women on the basis of vibration sense in toes (as graded by different observers) and red blood cell (RBC) count. Herein, we discuss several issues regarding these workplaces and associated cohorts, the endpoints evaluated, and the conclusions drawn by Li et al.1 First, several manufacturers of 1-bromopropane have performed a number of mammalian toxicology studies under good laboratory practice standards and according to validated testing guidelines. The route of exposure in these good laboratory practice studies was primarily but not exclusively via inhalation. Toxicity studies included acute, prenatal developmental, 28-day repeated concentration, 90-day repeated concentration, and two-generation reproduction. The US National Toxicology Program's Center for the Evaluation of Risks to Human Reproduction summarized and critically evaluated the aforementioned studies, along with studies performed in academic laboratories.2 On the basis of the results of the foregoing studies, safety data sheets were developed and are provided to customers. For example, Albemarle Corporation's Asia Pacific safety data sheet for 1-bromopropane states the following under the “Hazard Statements” and “Exposure controls/personal protection”:3 “Hazard Statements May damage fertility or the unborn child May cause drowsiness and dizziness Harmful: danger of serious damage to health by prolonged exposure through inhalation” “Exposure controls/personal protection Ventilation controls Use with local exhaust ventilation. Use only in well ventilated areas. Hygiene measures Avoid contact with skin, eyes and clothing. Wash thoroughly after handling. Personal protective equipment Wear suitable protective clothing, gloves and eye/face protection. Respiratory protective equipment Full facepiece approved respirator fitted with an organic vapour canister. The Albemarle Workplace Exposure Guideline of the major substance of the preparation is 25 ppm TWA8.” These measures are communicated to customers in the native language of the region to ensure the safe handling and use of this substance. In contrast, Li et al1 stated: “[n]one of the workers wore masks or gloves during the operation.” The authors also noted that “[t]he factory windows and doors were wide open during working hours, and a few exhaust fans were installed in each factory.” For one of the factories (ie, Yixing), Li et al1 cited to their previous description of this workplace, where they observed: “[i]n each plant, a ventilating fan was ineffectively installed 6 m from the floor; no local ventilation fan was installed in the vicinity of the areas where workers might be exposed to [1-bromopropane].”4 Li et al1 observed the following exposure opportunities in each of the three factories: “” adding of the chemicals into the reaction pots; sitting close to the reaction pots to observe and record the temperature; taking out the crude product; adding hydrogen carbonate and stirring; or pouring the product into the 1000-L drums. “In the final step, the workers added the product with hand scoops to adjust the product volume in the drum.” The situation noted earlier is troubling because the known hazards of 1-bromopropane have been properly communicated in the public domain, along with the necessary mechanical measures and personal protective equipment to ensure safe use. On the basis of the unacceptable practices reported by Li et al,1 the workers may have received significant exposures via the inhalation and dermal routes (eg, splashing). Though not reported by the authors, oral exposures might have also occurred if the workers were drinking and eating at their workstations, or contacting their hands with their mouths. Therefore, because airborne exposure levels were used as a surrogate for internal dose, this study may have suffered from underestimation of actual exposures by not considering dermal and oral exposures to the participants. Second, Li et al1 used various sex-, age-, and region-matched controls against the three cohorts, including workers from a beer factory (2001, Yixing), a refrigeration equipment factory (2003, Yixing), a knitting workshop (2004, Yixing), and a steel operation factory (2005, Weifang). No exposure monitoring data were provided on the unique types of exposures that the worker controls may have encountered in their specific industries or locales (eg, blood lead or methyl mercury levels). Chemical exposure information was limited to worker recall. Third, the following biochemistry, endocrinology, and hematology endpoints were reported as being statistically significantly different in exposed female workers and the controls: lactate dehydrogenase, thyroid stimulating hormone, follicle stimulating hormone, estradiol, white blood cell count, RBC count, hemoglobin, and hematocrit. Because several of these measures experience temporal fluctuations related to the menstrual cycle, due to hydration state, etc, Li et al1 should have obtained and controlled for these variables. This is particularly important considering that Li et al1 based their LAEL of 1.28 ppm, in part on RBC count in female workers. Furthermore, these types of measures can be subject to interlaboratory variation; therefore, the authors should have included information on the laboratory control specimens, along with the mean, standard deviation, and coefficient of variation, that were evaluated with each batch of worker samples. Providing normal clinical ranges for measures would have aided with further evaluating these types of data. The authors should also have considered data clustering associated with the plant and the laboratory. Ignoring data dependency generally inflates the significance level. Given the availability of exposure estimates for individual workers, the regression model should have been fit with respect to individual exposure of 1-bromopropane instead of the group median, and the benchmark dose then applied. The impact of grouping workers into low, medium, and high exposure on the LAEL is less unclear but masks the uncertainty of the LAEL. The lack of uniform linear dose–response trend in many of the health parameters (see Li et al's Tables 3 and 6) with respect to group median exposure raises the question of how well the linear regression model fit each health outcome with median exposure. Fourth, Li et al1 reported that tibial motor distal latency and sural nerve conduction velocity were statistically significantly increased or decreased, respectively, in female workers. As with the endpoints discussed previously, providing normal clinical ranges (eg, tibial motor distal latency, normal range = 2.1 to 6.0 ms; sural nerve conduction velocity, normal range = 47.48 to 58.84 m/s)5,6 for these values would have been helpful with evaluating the clinical significance of the reported statistically significant changes. Furthermore, the other endpoint from which Li et al1 based their LAEL of 1.28 ppm, that is, vibration sense in the toe of female workers, was statistically significantly associated with the examining, unblinded, neurologist. Fifth, the authors reported that fatigue on the profile of mood states scale was statistically significantly different in exposed female workers compared with controls. They also reported that vigor and confusion were statistically significantly different when the workers were categorized on the basis of cumulative exposure. Li et al1 also reported statistically significant differences in male workers for the Santa Ana test (nonpreferred hand). Because reference values for individuals from China have been published,7 providing normal reference ranges would have aided readers discriminating between clinically significant versus statistically significant differences. Furthermore, it is noteworthy that the World Health Organization's neurobehavioral core test battery (NCTB), which includes the profile of mood states and the Santa Ana tests, are confounded by education. Anger et al8 reported that “[t]he NCTB is effective in testing adults with more than 12 years of education, it is less effective in some populations with 8-10 years of formal education, but it can not reliably test persons with less education.”8 In their previous description of the Yixing workers, Ichihara et al4 identified four controls with an elementary school–level education and 19 with a junior high school–level education. In the 1-bromopropane–exposed workers, four had an elementary school–level education, 12 had a junior high school–level education, six had a high school–level education, and 1 had university-level education. Li et al1 did not report the level of education of the respective workers. Because the NCTB may be susceptible to floor effects, utilizing matched controls with limited education will not alleviate this source of bias.8 Finally, Li et al1 stated: “[t]he authors have no financial interest related to this research.” However, the corresponding author (Dr Gaku Ichihara) served as an expert witness in a plaintiffs' suit against a furniture manufacturer that used 1-bromopropane as an adhesive.9 This disclosure might have suggested a particular viewpoint to the readership of the Journal of Occupational and Environmental Medicine. In conclusion, Li et al1 evaluated 1-bromopropane exposures in three workplaces, where the mechanical controls were inadequate, and where personal protective equipments were either not used or fell woefully short of accepted practices, resulting in potentially higher levels of exposure than reported. The study evaluated a number of metrics subject to interobserver variability, but neither the researchers nor the research subjects were blinded, which calls into question the validity of the observer-graded and self-reported measures. The authors identified statistically significant changes in a number of endpoints, which they attributed to 1-bromopropane. However, no reference ranges were provided. This is particularly important in studies of this type, where it is difficult to obtain true controls. Notwithstanding the foregoing issues, we feel that the true value of Li et al's1 contribution is the importance of emphasizing compliance with occupational safety standards to optimize worker safety. Carr J. Smith, PhD Health, Safety and Environment Division, Albemarle Corporation, Baton Rouge, La Giffe T. Johnson, PhD Raymond D. Harbison, PhD Center for Environmental and Occupational Risk Analysis and Management, College of Public Health University of South Florida, Tampa, Fla Yiliang Zhu, PhD Department of Epidemiology and Biostatistics, College of Public Health University of South Florida, Tampa, Fla Richard V. Lee, MD Department of Medicine and Obstetrics State University of New York, Buffalo, NY Marek Banasik, MD, PhD Institute of Public Health and Environmental Protection, Warsaw, Poland Todd Stedeford, PhD, JD Health, Safety and Environment Division Albemarle Corporation, Baton Rouge, La

Telomere length (TL) is considered a marker of biological aging and has been associated with the presence of various coronary risk factors in patients. Much less is known about the relationships between TL and classic coronary risk factors in other populations. We measured TL in peripheral blood leukocytes of 343 middle-aged blood donors (mean age 40.2 ± 12.4 years; 201 men, 142 women) using quantitative polymerase chain reaction. Median TL was 0.86 (range: 0.48–1.85) relative TL units. In linear regression analyses with natural log-transformed T to S ratio as the dependent variable, there was a significant association with age (per year: beta = -0.007, p<0.001) and sex (males vs. females: beta = 0.075, p = 0.007) with longer telomeres in men. After adjusting for these two variables, we observed no association of TL with classic coronary risk factors including cholesterol (p = 0.36), triglyceride (p = 0.09), HDL-cholesterol (p = 0.26), LDL-cholesterol (p = 0.36), smoking (p = 0.97), and personal (p = 0.46) or family history (p = 0.63) of cardiovascular disease. However, we did find a significant positive association with white (p = 0.011) and red blood cell count (p = 0.031), hemoglobin (p = 0.014) and hematocrit (p = 0.013); we also found a borderline positive association with thrombocytes (p = 0.074). Positive associations remained significant for hemoglobin (p = 0.017), hematocrit (p = 0.023), and leukocytes (p = 0.009) in a subgroup with no reported vascular disease; associations were of borderline significance for erythrocytes (p = 0.053) and thrombocytes (p = 0.088) in this subgroup. The data do not support the concept that classic coronary risk factors contribute to telomere attrition in a blood donor population. However, telomere attrition may be a marker for reduced proliferation reserve in hematopoietic progenitor cells.

The hematological findings and the complaints of 1, 742 radiation workers in 229 industrial enterprises were investigated by the hematological examination records and the C.M.I. -Health Questionaire. As for the blood picture of participants, the mean values for the red blood cell counts (R.B.C.), white blood cell counts (W.B.C.) and hemoglobin contents of both male and female employees were found to be normal. Any particular changes of the elements of W.B.C. of 845 workers were not observed, while a slight decrease in the number of R.B.C. and W.B.C. of male workers could be recognized as their years of experience increased. In the scale of enterprises, R.B.C. and W.B.C. counts of the employees in the enterprises of small scale were significantly lower than those of the middle and the large scale ones. Number of workers who had R.B.C. counts of less than 4 million per mm3 in the group of the exposure dose of under 10 mrem per year was fewer than that of the group of 500 to 1, 000 mrem per year. The R.B.C. counts and hemoglobin contents of workers handling industrial gamma rays were lower than those of workers handling industrial x-rays, radioactive materials or medical x-rays. The W.B.C. counts of the industrial gamma ray and the medical x-ray groups were significantly lower than those of the industrial x-ray and the radioactive material groups. No particular differences in the classification of W.B.C. could be observed by classifying the scale of enterprises, kind of radiation sources, and exposure dose. The number of complaints of each radiation worker by the C.M.I. -Health Questionnaire, distributed as Polya-Eggenberger type. Ninety percent of the workers had less than 64 complaints by the C.M.I. -Health Questionnaire. There was no significant correlation between the number of complaints and the exposure dose. It seemed that the radiation workers had many complaints, especially on fatigue and uneasiness, compared with high school students as the control group.

Introduction: The productivity, quality of platelet apheresis collection has improved because of the considerable advancement in the automated cell separators. Automated cell separators have lot of sizeable scientific advances, but the alertness has been centered to Platelet Concentrates (PCs) quality than on safety of donor. Aim: To find the changes in haematological parameters and the consequences of apheresis and plateletpheresis on donor’s health. Materials and Methods: It was observational cross-sectional study done in laboratory at RL Jalappa Blood Bank, Tamaka, Kolar, Karnataka, India. The study was done from March 2019 to August 2020. A total of 300 healthy donors (plateletpheresis donors) were involved in the study. The plateletpheresis (Haemonetics MCS), predonation and postdonation haematological parameters such as haemoglobin concentration, Haematocrit (Hct), platelet, white and red blood cell count were calculated in all donors. The samples for Complete Blood Count (CBC) were secured from the donors, at the beginning and end of the procedure. Postdonation haematological parameters such as platelet count, haemoglobin, haematocrit, White Blood Cells (WBC), Red Blood Cells (RBC) counts of the donor was inscribed and comparison was done with the pre donation haematological parameters. Quality control of all Single Donor Platelet (SDP) products was done. All donors were evaluated for adverse donor reactions. The mean pre and post plateletpheresis values comparison was done utilising paired t-test. Statistical analysis was accomplished utilising Statistical Package for the Social Sciences (SPSS) software version 16.0. Results: Platelet count, haemoglobin, WBC count, RBC count and haematocrit were jotted down from 262 donors and a significant decrease was noticed in these parameters postdonation. Donor parameter platelet count (lac/mL) value was decreased from 273.57-224.28 whereas WBC count (cu/mm) predonation value decreased from 9.91-8.86 Postdonation, haemoglobin (g/dL) value decreased from 14.46-12.91, haematocrit (%) decreased slightly from 45.19-44.19, RBC count (million/mm3) decreased from 5.21-5.01. This concluded that the values decreased postdonation. Conclusion: The study conducted was safe from donor’s point of view. SDP is very effective in treatment of thrombocytopenia and is safe from recipient’s point of view.

The present study was aimed to assess the effects of dietary flaxseed supplementation in mitigating the toxic impacts of Bisphenol A on the haematological parameters of food fish Labeo rohita (rohu) (Hamilton, 1822). Bisphenol A, a chemical integral to plastic manufacturing, has potential to impact humans and aquatic fauna. The experiment involves three groups: A, B, &amp; C. The group A was served as control group, while group B was exposed to BPA dose and fed a standard diet. Group C received the same BPA dose as Group B but supplemented with a flaxseed- enriched diet. Blood samples were collected at 7th, 14th, and 21st days. Haematological parameters, including red blood cells count (RBC), haematocrit (hct) &amp; Haemoglobin concentration (Hb), platelets count, white blood cells (WBC) count and blood indices like mean corpuscular haemoglobin (MCH) &amp; mean corpuscular volume (MCV) were analysed. The BPA treated group shows significant (p &lt; 0.05) changes, reduction in values of RBC, Hb, Hct, MCV and Platelets, while the value of WBC and MCH get increased. Group C shows significantly (p &lt; 0.05) increase in Hb, RBC &amp; platelet count, Hct percentage and in MCV value from group B. The WBC count also get increase in group C as compare to control group. The study highlights that a flaxseed diet positively impacts hematological parameters in L rohita exposed to BPA toxicity. Incorporating bioactive compounds like flaxseed into fish diets enhances their health and resilience against waterborne toxicants. Additionally, hematological parameters are identified as reliable biomarker for assessing fish health in ecotoxicological research.