ABSTRACTIn this work, the adjoint to the transport operator is used to estimate the spatial distribution of an isotropic neutral particles source in a homogeneous one-dimensional medium, from readings of internal detectors. An analytical discrete ordinates formulation, the ADO method, is applied to derive a spatially explicit solution for the adjoint flux. Simulations are performed for reconstructing Gaussian and piecewise localized sources. Numerical results indicate that the source estimates may be considered satisfactory although scalar fluxes are reconstructed more precisely.