Optofluidic adaptive optics in multi-photon microscopy

Adaptive optics full-filed OCT (FFOCT) with a transmissive liquid crystal spatial light modulator (LCSLM) as wavefront corrector is used without strict plane conjugation for low order aberrations corrections. We validated experimentally that FFOCT resolution is independent of aberrations and only reduce the signal level. A signal based sensorless algorithm was thus applied for wavefront distortion compensation. Image quality improvements by the wavefront sensorless control of the LCSLM were evaluated on in vitro samples. By replacing the FFOCT sample arm objective with an artificial eye used to train ophthalmologists, adaptive optics retinal imaging was achieved. In vivo experiments using a liquid lens to correct focus and astigmatism are underway.

We present an open-loop adaptive optics (AO) system based on two liquid-crystal spatial light modulators (LCSLMs) that profit from high precision wavefront generation and good repeatability. A wide optical bandwidth of 300 nm is designed for the system, and a new open-loop optical layout is invented to conveniently switch between the open and closed loop. The corresponding control algorithm is introduced with a loop frequency (the reciprocal of the total time delay of a correction loop) of 103 Hz. The system was mounted onto a 2.16 m telescope for vertical atmospheric turbulence correction. The full width at half-maximum of the image of the star α Boo reached 0.636 arc sec after the open-loop correction, while it was 2.12 arc sec before the correction. The result indicates that the open-loop AO system based on LCSLMs potentially has the ability to be used for general astronomical applications.

Author Index to volume 202

Adaptive optics (AO) can efficiently compensate for turbulence-induced distortion in orbital angular momentum (OAM)-based optical wireless communication (OWC) systems. In this paper, we design a modified phase diversity algorithm (MPDA)-based wavefront sensor to enhance the reconstruction accuracy of distorted OAM wavefront information. Aiming to further strike a compelling trade-off between AO system complexity and compensation accuracy, we first construct a novel AO system that applies a quickly and electronically controlled focus-tunable lens (FTL). It decontaminates distorted OAM signaling beams while having a low systemic complexity and superior convergence performance. Furthermore, we propose the 3-modified phase diversity algorithm (3-MPDA) AO scheme relying upon a Fourier intensity and two defocused intensities as the prior information, which beneficially balances the compensation effect and the number of defocused intensities and exhibits good noise robustness against charge-coupled device (CCD) detectors. In summary, this paper provides new insight for designing AO schemes with high compensation performance in communication links.

A Full-Field OCT (FFOCT) setup coupled to a compact transmissive liquid crystal spatial light modulator (LCSLM) is used to induce or correct aberrations and simulate eye examinations. To reduce the system complexity, strict pupil conjugation was abandoned. During our work on quantifying the effect of geometrical aberrations on FFOCT images, we found that the image resolution is almost insensitive to aberrations. Indeed if the object channel PSF is distorted, its interference with the reference channel conserves the main feature of an unperturbed PSF with only a reduction of the signal level. This unique behavior is specific to the use of a spatially incoherent illumination. Based on this, the FFOCT image intensity was used as the metric for our wavefront sensorless correction. Aberration correction was first conducted on an USAF resolution target with the LSCLM as both aberration generator and corrector. A random aberration mask was induced, and the low-order Zernike Modes were corrected sequentially according to the intensity metric function optimization. A Ficus leaf and a fixed mouse brain tissue slice were also imaged to demonstrate the correction of sample self-induced wavefront distortions. After optimization, more structured information appears for the leaf imaging. And the high-signal fiber-like myelin fiber structures were resolved much more clearly after the whole correction process for mouse brain imaging. Our experiment shows the potential of this compact AO-FFOCT system for aberration correction imaging. This preliminary approach that simulates eyes aberrations correction also opens the path to a simple implementation of FFOCT adaptive optics for retinal examinations.

Spatial beam shaping can be achieved using wavefront modulators to increase ultrafast laser processing efficiency. These modulators can display a pre-calculated phase mask on the beam path in order to shape the laser intensity distribution following a user defined target in the processing plane or volume. Due to the non-perfect optical response of wavefront modulators, the experimental distribution may differ from the target. We investigate the use of electrically addressed and optically addressed liquid crystal spatial light modulators with ultrafast laser pulses. Applications in parallelized surface and bulk processing are achieved with both modulators showing the advantages and drawbacks of these technologies. In particular, we focus on the limitations of these devices in terms of spatial and phase delay resolution, showing the consequences on the shaped beam distribution. Calculation strategies to overcome these limitations are discussed.

Liquid crystal (LC) based spatial light modulators (SLM) offer a low cost alternative to the expensive deformable mirrors used in most adaptive optics systems. Real time correction of simulated atmospheric turbulence is demonstrated. The corrective element is a 10 by 10 pixel ferroelectric liquid crystal (FLC) SLM. An improvement in the Strehl ratio from 23% to 33% with a frame rate of 6Hz was demonstrated. A nematic liquid crystal (NLC) SLM was used to generate realistic atmospheric turbulence.

Adaptive optics systems have been integrated in many imaging modalities in order to correct for aberrations that are introduced by samples and optical elements. Usually, the optical system has access to a guide star (i.e., a point-like structure that is smaller than the diffraction limit). This guide star can be used as a beacon for adaptive optics enhancement. In contrast, for spectroscopy and densely-labeled fluorescent samples, the signal is diffused throughout the entire beam path and is not confined to a well-defined point-like structure. Here, we show analytically and experimentally that, in these scenarios, adaptive optics systems are expected to yield significantly lower signal enhancement than when a guide star is available. We discuss adaptive optics' performance degradation for different imaging modalities (e.g., confocal, multi-photon microscopy) and identify solutions to overcome low signal enhancements.

(abridged) Extreme adaptive optics (XAO) encounters severe difficulties to cope with the high speed (>1kHz), high accuracy and high order requirements for future extremely large telescopes. An innovative high order adaptive optics system using a self-referenced Mach-Zehnder wavefront sensor (MZWFS) allows counteracting these limitations. This sensor estimates very accurately the wavefront phase at small spatial scale by measuring intensity differences between two outputs, with a $\lambda /4$ path length difference between its two legs, but is limited in dynamic range due to phase ambiguity. During the past few years, such an XAO system has been studied by our team in the framework of 8-meter class telescopes. In this work, we report on our latest results with the XAO testbed recently installed in our lab, and dedicated to high contrast imaging with 30m-class telescopes (such as the E-ELT or the TMT). After reminding the principle of a MZWFS and describing the optical layout of our experiment, we will show the results of the assessment of the woofer-tweeter phase correctors, i.e., a Boston Micromachine continuous membrane deformable mirror (DM) and a Boulder Nonlinear Systems liquid crystal spatial light modulator (SLM). In particular, we will detail the calibration of the DM using Zygo interferometer metrology. Our method consists in the precise measurement of the membrane deformation while applying a constant deformation to 9 out of 140 actuators at the same time. By varying the poke voltage across the DM operating range, we propose a simple but efficient way of modeling the DM influence function using a Gaussian model. Finally, we show the DM flattening on the MZWFS allowing to compensate for low order aberrations.

Binary adaptive optics involves using a simple two-state algorithm for wavefront correction. The technique lends itself to implementation using ferroelectric liquid crystal spatial light modulators (FLC-SLMs) as half-wave and quarter-wave phase shifters. In this paper we demonstrate further the effect of half-wave and quarter-wave correction, we compare the ideal performance with conventional analogue adaptive optics and we discuss the relevant optical properties of FLC-SLMs.

We present results of adaptive optics compensation at the receiver of a 3km optical link using a beacon laser operating at 635nm. The laser is transmitted from the roof of a seven-storey building over a near horizontal path towards a 127 mm optical receiver located on the second-floor of the Applied Optics Group at the National University of Ireland, Galway. The wavefront of the scintillated beam is measured using a Shack-Hartmann wavefront sensor (SHWFS) with high-speed CMOS camera capable of frame rates greater than 1kHz. The strength of turbulence is determined from the fluctuations in differential angle-of-arrival in the wavefront sensor measurements and from the degree of scintillation in the pupil plane. Adaptive optics compensation is applied using a tip-tilt mirror and 37 channel membrane mirror and controlled using a single desktop computer. The performance of the adaptive optics system in real turbulence is compared with the performance of the system in a controlled laboratory environment, where turbulence is generated using a liquid crystal spatial light modulator.

Wavefront reconstruction method based on wavelet fractal interpolation for coherent free space optical communication

We discuss the design and implementation of a low-cost, high-resolution adaptive optics test-bed for vision research. It is well known that high-order aberrations in the human eye reduce optical resolution and limit visual acuity. However, the effects of aberration-free eyesight on vision are only now beginning to be studied using adaptive optics to sense and correct the aberrations in the eye. We are developing a high-resolution adaptive optics system for this purpose using a Hamamatsu Parallel Aligned Nematic Liquid Crystal Spatial Light Modulator. Phase-wrapping is used to extend the effective stroke of the device, and the wavefront sensing and wavefront correction are done at different wavelengths. Issues associated with these techniques will be discussed.

Using the liquid crystal spatial light modulator (LC-SLM) as the wavefront corrector, an open-loop adaptive optics (AO) system for fundus imaging in vivo is constructed. Compared with the LC-SLM closed-loop AO system, the light energy efficiency is increased by a factor of 2, which is helpful for the safety of fundus illumination in vivo. In our experiment, the subjective accommodation method is used to precorrect the defocus aberration, and three subjects with different myopia 0, -3, and -5 D are tested. Although the residual wavefront error after correction cannot to detected, the fundus images adequately demonstrate that the imaging system reaches the resolution of a single photoreceptor cell through the open-loop correction. Without dilating and cyclopleging the eye, the continuous imaging for 8 s is recorded for one of the subjects.

In space optical communication, owing to the influence of atmospheric turbulence, optical beams lose focus and become phase-distorted, which reduces the communication quality. Considering the polarization dependence of liquid crystal spatial light modulators and the dispersion effect of liquid crystal materials, the energy utilization rate of liquid crystal adaptive optics systems is low. In this study, a dual liquid crystal spatial light modulator adaptive optics system based on the GS algorithm is used to correct the wavefront distortion of a signal beam under different atmospheric turbulence intensities, and the Strehl ratio (SR) is used as the evaluation index. The simulation results show that the SR of the corrected system can be increased from 0.23, 0.41, and 0.72 to 0.77, 0.89, and 0.95, respectively. The corrected beam spot was more concentrated and the light intensity at the center of the beam spot was stronger. The experimental results show that, after the distortion wavefront is corrected by the dual liquid crystal spatial light modulator, the average gray value of the 10 × 10 pixels in the center of the spot increases from 159.3, 113.1, and 58.4 to 253.4, 247.7, and 198.3, respectively.