PURPOSE: to define the standard operating procedure for collecting isolate metadata using BioNumerics for submission of food/environmental isolates to NCBI. SCOPE: to provide a standardized procedure to collect isolate metadata using BioNumerics for submission of food/environmental isolates to NCBI. RESPONSIBILITIES- SOP Responsible Officials: Ruth Timme, Maria Balkey The GenomeTrakr Network Management will be responsible to monitor GenomeTrakr submissions processed through Bionumerics and ensure that all GT labs are familiar with the mandatory metadata fields required for submission of GenomeTrakr sequencing records to NCBI. V3: Added dropdown menus from controlled vocabulary to sequenced by and project name to metadata template PulseNet_Bionumerics_Isolate_Metadata V4: Changes in metadata template PulseNet_Bionumerics_Isolate_Metadata. - Added dropdown menus from controlled vocabulary to collected_by , SourceCountryState - Added fields: collected by, isolation source - Added mapping table of attribute names. - Remove requirement to send biosample update to NCBI to make changes on sequenced by and project name.