Seeing the locust in the swarm: accounting for spatiotemporal hierarchy improves ecological models of insect populations

In hierarchical patch systems, small-scale patches of high density are nested within large-scale patches of low density. The organization of multiple-scale hierarchical systems makes non-random strategies for dispersal and movement particularly important. Here, we apply a new method based on first-passage time on the pathway of a foraging seabird, the Antarctic petrel (Thalassoica antarctica), to quantify its foraging pattern and the spatial dynamics of its foraging areas. Our results suggest that Antarctic petrels used a nested search strategy to track a highly dynamic hierarchical patch system where small-scale patches were congregated within patches at larger scales. The birds searched for large-scale patches by traveling fast and over long distances. Once within a large-scale patch, the birds concentrated their search to find smaller scale patches. By comparing the pathway of different birds we were able to quantify the spatial scale and turnover of their foraging areas. On the largest scale we found foraging areas with a characteristic scale of about 400 km. Nested within these areas we found foraging areas with a characteristic scale of about 100 km. The large-scale areas disappeared or moved within a time frame of weeks while the nested small-scale areas disappeared or moved within days. Antarctic krill (Euphausia superba) is the dominant food item of Antarctic petrels and we suggest that our findings reflect the spatial dynamics of krill in the area.

Future climate change likely to reduce the Australian plague locust (Chortoicetes terminifera) seasonal outbreaks

To know the role of meteorological systems of synoptic scale in outbreaks of desert locust (Schistocerca gregaria Forsk) at various phases of its life cycle in western India, data on locust situation and synoptic disturbances during period from 1945 to 1980 were analysed.
 The analysis of the fortnightly data of the infestation of egg layings, hoppers, scattered adults and swarms for seven stations shows that the chronological orders of these activities follow the spatial progress of the normal monsoon onset with pre-monsoon rains. However, the highest swarm activity was found in the first fortnight of August.
 Simple linear correlation technique was used to know the degree of association of various meteorological disturbances with locust activity at different stages for a station with good record of data set. The egg-laying activity was parallel with rainfall and slight decrease in rainfall between peaks did not affect the activity- Hopper emergence peaks were seen generally after a month of rainfall. Scattered adults activity showed no good association with rainfall. The swarms intensity was highest during first fortnight of August and September. The main meteorological systems causing rainfall were western disturbances, depressions over Arabian Sea and a few depressions developed over land.
 It was also found that a negative correlation exists between swarm infestation and sunspot number.

Most fluvial systems exhibit systematic, continuous upstream to downstream variations in channel morphology and related ecological and hydrological parameters (emphasized by conceptual frameworks such as downstream hydraulic geometry and the river continuum concept), and discontinuous, shorter range variations (emphasized by hierarchical patch dynamics). This study investigates the relative importance of broader-scale upstream to downstream variation and local variation at the hydraulic unit scale in a bedrock-controlled stream in central Kentucky, USA. A nested ANOVA analytical approach was used to determine the relative importance of three nested spatial scales in explaining variations in channel morphology and riparian trees. Results show that channel morphology is largely controlled by local-scale variation explaining about 92% of slope, 46% of bankfull width, 99% of average depth, 54% of width-depth ratio, 86% of channel cross-sectional area, and 100% of the hydraulic radius of the channel. Different categories of substrate characteristics, however, represent anomalies with respect to variance explained at different levels. Furthermore, local-scale controls explain 60% of variations in species richness, 59% of variations in the total number of individual trees, 68% of variation in the proportion of Platanus occidentalis basal area and 43% of variation in the total number of biogeomorphic impacts. These results are consistent with the idea of tight coupling between channel morphology and riparian vegetation, although they do not, by themselves, prove such interactions. The morphological variation of the channel at the local scale is primarily attributable to the geological controls (e.g., faults, bedding planes, joints and fractures) and incision status associated with the study area. The local scale variation in vegetation pattern can be explained by the highly local edaphic differences along the riparian corridor, which is likely to be related to the local scale fluvial process-form variations, and biogeomorphic impacts and feedbacks. These patterns may therefore be common in bedrock rivers strongly influenced by geological controls.

A WIDESPREAD outbreak of the migrating grasshopper, Chortoicetes terminifera, Walk., occurred in Australia during 1933–35. Reports show that, in South Australia, small, local swarms appeared during the summer 1932–33 in certain districts in the pastoral country about 150 miles north of the 10 in. annual isohyet (see Fig. 1). During 1933–34 swarms were widely distributed and in some areas extended about 100 miles south of this rainfall line. During 1934–35 they became generally distributed southwards over the agricultural areas of the State. These widespread invasions develop only at irregular intervals of several years; the presence of the insects in numbers, in the southern areas of the State, is temporary. Restricted outbreaks occur more frequently in certain areas situated immediately north of the 10 in. isohyet; in some instances the swarms may extend southwards into the wheat belt.

Processed Landsat multispectral data were used to determine areas in the Riverina region of New South Wales, Australia, in which eggs were likely to have been laid by swarms of the Australian plague locust ( Chortoicetes terminifera (Walker)), which invaded the region at the end of March 1987. A map was produced from the processed data with potential eggbed areas highlighted, which could be used for field survey purposes in the spring and summer when hatching started. The results showed that > 95 per cent of all nymphal band targets were in the areas defined by a normalized difference vegetation index as being those where vegetation growth had resulted from rain during March. Furthermore, a maximum-likelihood classification using a known eggbed location as a training area, produced an excellent correlation with locusts hatching in September/October 1987. The results are discussed in relation to the future operational use of such techniques by the Australian Plague Locust Commission.

A dozen species of locusts (Orthoptera: Acrididae) are a major threat to food security worldwide. Their outbreaks occur on every continent except Antarctica, threatening the livelihood of 10% of the world’s population. The locusts are infamous for their voracity, polyphagy, and capacity for long-distance migrations. Decades of research revealed very complex bio-ecology of locusts. They exist in two, inter-convertible and density-dependent states, or “phases.” Despite the evident progress in understanding locust behavior, our ability to predict and manage locust outbreaks remains insufficient, as evidenced by locust plagues still occurring during the 21st century. One of the main reasons is that locusts typically inhabit remote and scarcely populated areas, and their distribution ranges often spread across continents. This creates tremendous obstacles for locust population monitoring and control. Traditional ground locust surveys are inadequate to address the enormous spatial scale of the locust problem in a limited window of time dictated by the pest’s development. Remote sensing (satellite information) appears a promising tool in locust monitoring. Satellite data are increasingly used for monitoring and forecasting two locust species, the desert and the Australian plague locust. However, applications of this geospatial technology to other locust species remain rare.

Many pest species exhibit huge fluctuations in population abundance. Understanding their large-scale and long-term dynamics is necessary to develop effective control and management strategies. Occupancy models represent a promising approach to unravel interactions between environmental factors and spatiotemporal dynamics of outbreaking populations. Here, we investigated population dynamics of the Australian plague locust, Chortoicetes terminifera, using density data collected between 1988 and 2010 by the Australian Plague Locust Commission over more than 3 million km2 in eastern Australia. We applied multistate and autologistic multi-season occupancy models to test competing hypotheses about environmental and demographic processes affecting the large-scale dynamics of the Australian plague locust. We found that rainfall and land cover predictors best explained the spatial variability in outbreak probability across eastern Australia. Outbreaks are more likely to occur in temperate than tropical regions, with a faster and more continuous response to rainfall in desert than in agricultural areas. Our results also support the hypothesis that migration tends to propagate outbreaks only locally (over distances lower than 400 km) rather than across climatic regions. Our study suggests that locust outbreak forecasting and management systems could be improved by implementing key environmental factors and migration in hierarchical spatial models. Finally, our modeling framework can be seen as a step towards bridging the gap between mechanistic and more phenomenological models in the spatial analysis of fluctuating populations.

Locusts and grasshoppers cause considerable economic damage to agriculture worldwide. The Australian Plague Locust Commission uses multiple pesticides to control locusts in eastern Australia. Avian exposure to agricultural pesticides is of conservation concern, especially in the case of rare and threatened species. The aim of this study was to evaluate the probability of pesticide exposure of native avian species during operational locust control based on knowledge of species occurrence in areas and times of application. Using presence-absence data provided by the Birds Australia Atlas for 1998 to 2002, we developed a series of generalized linear models to predict avian occurrences on a monthly basis in 0.5 degrees grid cells for 280 species over 2 million km2 in eastern Australia. We constructed species-specific models relating occupancy patterns to survey date and location, rainfall, and derived habitat preference. Model complexity depended on the number of observations available. Model output was the probability of occurrence for each species at times and locations of past locust control operations within the 5-year study period. Given the high spatiotemporal variability of locust control events, the variability in predicted bird species presence was high, with 108 of the total 280 species being included at least once in the top 20 predicted species for individual space-time events. The models were evaluated using field surveys collected between 2000 and 2005, at sites with and without locust outbreaks. Model strength varied among species. Some species were under- or over-predicted as times and locations of interest typically did not correspond to those in the prediction data set and certain species were likely attracted to locusts as a food source. Field surveys demonstrated the utility of the spatially explicit species lists derived from the models but also identified the presence of a number of previously unanticipated species. These results also emphasize the need for special consideration of rare and threatened species that are poorly predicted by presence-absence models. This modeling exercise was a useful a priori approach in species risk assessments to identify species present at times and locations of locust control applications, and to discover gaps in our knowledge and need for further focused data collection.

An important outbreak of the Australian plague locust, Chortoicetes terminifera (Wlk.), occurred in 1973 in central western New South Wales. The dynamics of the outbreak could be resolved into a sequence of five principal interacting events: (1) Sustained population increases in low-density populations in northern central western New South Wales during three successive breeding periods in 1972. The increases were a result of successful, local multiplication, immigration from more northerly breeding areas and restricted opportunities for dispersal. The continuing concentration of locusts in the northern central west led to local gregarisation and swarm formation in autumn and spring 1972. (2) Massive southward displacements at night during an intense tropical disturbance between 29 January and 4 February 1973, which resulted in an invasion and concentration of infestations in southern central western New South Wales. (3) Substantial multiplication increases (10- to 30-fold), as a result of the drought-breaking rainfall in the autumn and spring of 1973 in the central-west. This resulted in an intensification of the plague with the production of numerous, slow-moving swarms. (4) Massive southward displacements during several periods of disturbed weather in spring 1973, which led to the disappearance of adult infestations from the central west and a dispersal of infestations in areas invaded further south. (5) Heavy mortality in residual populations of hoppers and eggs. The combined effects of parasites, predators and a reduced viability of eggs and hoppers resulted in a complete collapse of the plague in the central west by December 1973.Although displacement by adults at night is shown to be the principal factor regulating both population fluctuations in specific localities and the spread of plagues through southward invasions, outbreaks are ultimately determined by initial multiplication increases in relatively sedentary recession populations. Both multiplication and migration were influenced more by variations in synoptic weather than by any other factor. Diurnal swarm movements, although conspicuous, did not significantly influence the overall spread of the outbreak studied.The quality and quantity of available fresh green food did not appear to limit further successful breeding during the decline of the plague. Although influxes of locusts into the central-west continued during disturbed weather in 1974, none remained to breed and densities fell to levels (<1/ha) lower than those of drought seasons. It appears unlikely, therefore, that plagues can be sustained in any one area, even when environmental conditions remain favourable for breeding, because of: (1) mass emigration during disturbed weather; (2) increased effectiveness of natural enemies; and (3) declines in viability and fecundity in moist conditons.

Outbreaks of the Australian Plague Locust

A field experiment was tested in the Central Laboratory for Agricultural Climate Experimental Farm, Dokki, Giza Governorate, Egypt, during the two growing seasons of 2019/2020 and 2020/2021 on cauliflower. The aim of this research was to study the effect of plant spacing foliar spraying with different concentrations of application of boron (B) on the vegetative growth, chemical content and curds yield of cauliflower cv. Amshiry under field conditions. Plants were sprayed with 50, 100 and 200 ppm at 20, 40, 60 and 80 days after transplanting, whilst the in-row plant spacing was 0.3 and 0.5 m between plants. Results showed that boron foliar application significantly improved vegetative growth parameters, curds yield and its components and chemical composition of leaves and cauliflower plant. Likewise, using 0.50 m plant spacing significantly enhanced plant fresh weight, plant height, leaves fresh weight and leaves dry weight and chemical composition of leaves and curds. The highest yield per plant was obtained by plant spacing 0.5m combined with 200 ppm boron followed by plant spacing 0.50 m combined with 100 ppm boron. On the other hand, the production from land units increased by using 0.3 m in-row plant spacing while the production of individual plants was less than 0.5m treatment. The 200 ppm boron application gave the highest net profit per feddan followed by 100 ppm. The lowest net profit was obtained by 0.30 m plant spacing combined with control treatment.

The sole Na+ /H+ antiporter Nhx1 has been generally unexplored in filamentous fungi. We characterized Nhx1 in the entomopathogenic fungus Beauveria bassiana. An eGFP-tagged Nhx1 fusion accumulated in small punctuate structures, presumably endosomal and trans-Golgi network compartments, between septum and tubular vacuole of each wild-type cell stained with a vacuole-specific dye. Deletion of nhx1 resulted in significant acidification and severe fusion defect in vacuoles, which were fragmented and distinct from large or tubular wild-type vacuoles. The deletion also caused a drastic reduction in aerial conidiation or submerged blastospore production and more severe defect in vegetative growth than in conidial germination. The Δnhx1 mutant became more sensitive to high osmolarity, heat shock and several metal ions during growth but its conidia showed increased UV-B tolerance. Intriguingly, Δnhx1 was unable to infect a model insect through cuticle penetration or intrahaemocoel injection because it produced much less biomass and cuticle-degrading enzymes in a minimal broth and failed to form blastospores in the insect haemolymph. All changes were completely or largely restored by targeted nhx1 complementation. Our results provide novel insight into an indispensability of Nhx1 for not only vacuolar fusion but also life cycles in vitro and in vivo in B. bassiana.