Shaping efficiency versus dentin conservation: a micro-CT taper-based analysis.

ABSTRACTRoot canal morphology and its anatomical variations pose a great challenge to endodontistsAimThe aim of this in silico study was to perform a qualitative and quantitative analysis of the threedimensional morphological characteristics of the isthmus in the mesial root canals of mandibular molars using microcomputed tomography (micro-CT)Material and MethodSix hundred first mandibular molars were selected, including 317 with two mesial canals with isthmuses between the canals, and fully formed root. Isthmus morphology was determined in 3D longitudinal sections using Fan et al. (2010) classification. Root length, and the volume and area of apical and coronal level were measured. Additionally, the structural model index (SMI) of the canals were also assessedResultsThe prevalence of isthmuses in the mesial root canals was 32% type II, 29% type III, 22% type IV, and 17% type I. The root length was found to be 9.1±0.5 mm, the volume and area, of all root canal system, were 41.8±40.1 mm3 and 63.6±24.2 mm2 respectively. The isthmi volume and area alone were 11.06±9.03 mm3 and 30.02±11.02 mm2. The study confirmed that isthmuses are present in mesial canals of mandibular first molars, being more frequent in the apical thirdConclusionThe high prevalence of isthmuses with complex morphological features underscores the importance of using intracanal medications to disinfect areas unprepared by instruments.

The Impact of TruNatomy and ProTaper Gold Instruments on the Preservation of the Periradicular Dentin and on the Enlargement of the Apical Canal of Mandibular Molars

The aim of this study was to compare the filling capacity in curved root canal using a new continuous wave of condensation technique (Termo Pack II, Easy Dental Equipments, Brazil) or lateral compaction. The percentage of voids in the filling of mesial root canals of mandibular molars was assessed by micro-computed tomography (micro-CT). Mesial root canals (n=24) of mandibular molars with a degree of curvature between 20° and 40° were prepared using rotary system (ProDesign Logic, Easy, Brazil) up to #35, .05 taper. The root canals were filled by using the continuous wave of condensation system or lateral compaction and AH Plus sealer (n=12). Scanning at 9 µm was performed after preparation and after filling by using micro-CT SkyScan 1176. The volumetric percentage of filling material and voids (total length and in each root canal third) were calculated. Data were analyzed using ANOVA/Tukey and Student’s t tests (α=0.05). Before the filling techniques, the root canals volume after preparation was similar (p>0.05). The root canals filled by the continuous wave of condensation technique presented the lowest percentage of voids, and the greatest percentage of filling material in total length and thirds (cervical, middle and apical) (p<0.05). Both techniques were not able of completely filling the root canals. The continuous wave of condensation technique Termo Pack II promoted better root canal filling in curved root canals, when compared with lateral compaction.

To evaluate, using micro-CT, the remaining dentine thickness after preparation of the mesiobuccal (MB), mesiolingual (ML) and middle mesial (MM) canals of mandibular first molars with the ProTaper Next rotary system. Eleven mesial roots of mandibular first molars having three independent canals from the furcation level up to at least 5mm towards the apical direction were selected. Preparation of MM canals was performed in two steps using ProTaper Next X2 (step 1) and X3 (step 2) instruments, whilst MB and ML canals were prepared in a single step up to X3 instruments. The roots were scanned (pixel size of 10µm) before and after each step, and the dentine volume was calculated. Postoperative models of the roots were coregistered with their preoperative dataset and colour-coded cross-sections of the roots were used to measure the smallest dentine thickness surrounding each canal at 1.0-mm intervals from the furcation level up to 5mm in the apical direction, in both mesial and distal aspects of the roots. Changes in the remaining wall thickness between mesial canals were analysed with repeated-measures anova and post hoc Tukey test. Significance level was set at 5%. Mean percentage reduction of dentine volume after steps 1 and 2 was 4.66% and 5.16%, respectively. Overall, pre- and postoperative dentine thickness of the MM canal walls, in both mesial and distal aspects of the root, were significantly thinner than those of MB and ML canals (P<0.05). No significant difference was observed when comparing dentine thickness in the mesial and distal directions of MM canal after preparation step 1 (0.88±0.18mm and 0.73±0.26mm, respectively) or 2 (0.83±0.17mm and 0.67±0.26mm, respectively) (P >0.05). Dentine thickness values less than 0.5mm were observed mostly towards the distal aspect of the MM canal. Mesial roots were not associated with strip perforations after canal preparation procedures. A significant decrease in the thickness of canal walls at all levels evaluated in the mesial roots of mandibular molars suggests that files with small tapers should be used in preference to instruments with large tapers to prepare mesial canals in mandibular molars.

Objective: The aim of this study was to investigate the accuracy of endoscopic visualization to detect root canal anastomoses at the coronal half of the mesial root canals of mandibular molars using micro-computed tomographic (micro-CT) images as reference.Material and methods: Seventy-four mesial roots of mandibular first molars with (n = 47) or without (n = 27) intercanal anastomosis were selected based on the micro-CT scans of 269 mandibular first molars at a pixel size of 10 µm. The specimens were mounted on the mannequins and their root canals were evaluated using dental operating microscope (DOM) and endoscope. The endoscopic probe was inserted into the main mesial root canals and 2 blinded observers evaluated the presence of a divergence point of anastomosis (where the branching occurs) as ‘present’ or ‘absent’. The scorings were compared with the three-dimensional reconstructed images of the specimens and recorded as ‘correct’ or ‘incorrect’ evaluation. Degree of agreement between evaluators was assessed with Kappa test and the accuracy of endoscopic visualization according to the presence and location of anastomosis was compared using Fisher exact tests with a significance threshold at 5%.Results: High inter-examiner reliability was reported (0.91). None of the divergence points were identified using DOM whereas 11 divergence points were detected using endoscope, corresponding the 23.4% of the intercanal anastomoses. The endoscope also showed the absence of an intercanal anastomosis correctly in all of the specimens without an anastomosis. Detectability of a divergence point using endoscope was not affected by its location within the coronal half of root canal (p > .05).Conclusions: The endoscopes were able to visualize the divergence points of 23.4% of the intercanal anastomoses located at the coronal halves of root canals.

To analyze the ability of two manual instrumentation techniques to center mesial root canals of mandibular molars, studied by computed microtomography (micro-CT). Twenty mesial root canals of mandibular molars were matched based on similar morphological dimensions using micro-CT evaluation and divided in 2 groups (n=10): (1) Crown-down technique with Gates-Glidden drills and K-Flexofiles and (2) M manual NiTi rotary system. Changes in volume, surface area and canal transportation were compared using an unpaired t-test with a 5% significance level. No significant differences were observed between groups regarding volume surface area after root canal preparation (p>0.05). Variation in the centroid differed between groups in the total canal length, and in the cervical and middle thirds, with better centralization for the M files (p<0.05). Both manual instrumentation techniques had similar volume and surface area variation. Both techniques left unprepared canal areas with similar values. M manual NiTi files caused minor canal transportation.

To compare the efficacy of ultrasonically activated irrigation (UAI), photon-induced photoacoustic streaming (PIPS) and shock wave enhanced emission photoacoustic streaming (SWEEPS) activation for the removal of accumulated hard-tissue debris (AHTD) from the root canal system of mandibular molars when assessed using microcomputed tomography (micro-CT). A total of 30 mandibular first and second molars with joining mesial root canals containing an isthmus and a single distal canal were subjected to three micro-CT scans (before and after canal instrumentation and after final irrigation) at a resolution of 15μm. Mesial canals were prepared up to aProTaper F3 rotary file, and distal canals were prepared up to aProTaper F4 rotary file. Teeth were randomly assigned to three groups according to the irrigant activation method (n=10): the UAI group, the PIPS group and the SWEEPS group. The final irrigation procedures were performed using a total of 15.5mL of 1% NaOCl for each tooth with an activation time of 3×30s. After three-dimensional model reconstruction and volumetric measurement of root canals, the percentage reduction (%Rd) of AHTD was calculated. The %Rd of AHTD was analysed statistically using one-way analysis of variance and nonparametric tests at a significance level of 5%. There were no significant differences between the three groups in terms of canal volume before or after instrumentation, or the volume of debris after canal preparation (P>0.05). In the mesial canals, irrigation with SWEEPS reduced the overall debris by 84.31%, which was significantly more than the reduction associated with PIPS and UAI (58.79% and 50.27%, respectively). In the distal canals, the %Rd of AHTD was significantly different between PIPS and SWEEPS and between UAI and SWEEPS (P<0.05), but was not significantly different between PIPS and UAI groups (P>0.05). The SWEEPS was associated with a greater %Rd of AHTD than the PIPS and UAI groups. SWEEPS was associated with significantly less debris compared than PIPS and UAI, especially in isthmus-containing mesial roots. None of the activation techniques completely removed debris from root canal systems.

This study evaluated unprepared canal areas, volume of dentine removal, and dentine thickness after the progressive enlargement of the buccal canals of 22 maxillary molars, with and without the MB2, using instruments with the same tip size (0.25 mm) but 4 different tapers (0.03, 0.05, 0.06 and 0.08 v). Micro-CT scans were performed after each instrument. Data were compared using a general linear mixed model post hoc comparisons with Kenward-Roger for Wald F tests (α = 5%). After each treatment step, it was observed an increase in the percentage volume of removed dentine and a decrease in the unprepared areas and dentine thickness at the pericervical region of all canals (p < 0.05). The percentage reduction of dentine thickness in MB2 canal was higher than in the mesiobuccal and distobuccal canals (p < 0.05). Knowledge of pre-operative dimensions and dentine thickness before enlargement of buccal canals of maxillary molars is an important factor to avoid excessive dentine removal.

The risk of furcal perforation in mandibular molars using Gates-Glidden drills with anticurvature pressure

Introduction: The purpose of this article is to emphasize on the thorough knowledge of root canal anatomy. Methods: This case report describes the endodontic treatment of mandibular first molar with seven root canals (five mesial and two distal root canals) which was confirmed using angulated radiographs and computed tomography (CT-scan) scanning. Results: CT scan and radiographs showed that both mesiobuccal and mesiolingual root have Vertucci type IV canal pattern, whereas distal root showed Vertucci type II canal configuration. Conclusion: This case report describes the endodontic treatment of mandibular first molar with five mesial and two distal canals, with emphasis on rate of occurrence of multiple canals, and the importance of their identification and treatment.

To evaluate the influence of traditional and conservative access cavity preparations on the remaining dentine thickness in the coronal third of mesial canals of extracted mandibular molars prepared with reciprocating instruments using micro-computed tomography as the analytical tool. Seventy extracted mandibular molars were scanned at a pixel size of 19μm. From this initial sample, 20 teeth were selected, pair-matched and distributed into two groups (n=10) according to the access cavity preparation: traditional (TradAC) or conservative (ConsAC). The root canals were sequentially enlarged with Reciproc Blue R25 (size 25, 0.08v taper) and R40 (size 40, 0.06v taper) instruments. A new scan was performed and the postoperative stacks were coregistered with their respective preoperative datasets. A colour-coded cross sections of the roots were created and used to identify and measure the smallest dentine thickness related to both MB and ML canals at 1.0-mm intervals from the furcation level of up to 5mm in the apical direction, in both mesial and distal aspects of the roots, before and after preparation. The statistical analyses were performed with paired-samples t-test, independent-samples Student T-test and Chi-Square test with a significance level of 5%. At all levels of both groups, dentine thickness before preparation was greater than after preparation (p<.05). No difference in the percentage of dentine reduction was observed between TradAC and ConsAC groups (p>.05), but a significantly greater reduction was observed to the distal aspect of the roots (p<.05). After root canal preparation, dentine thinner than 0.5mm was observed mostly along the distal aspect of the root (10% to 15%) of the MB and ML canals, with no influence of the access cavity type on its incidence to either mesial (X2 =1.66; p=.2) or distal (X2 =0.40; p=.5) directions. In the TradAC group, dentine thickness in most slices was greater than 1.0mm after preparation (n=124) whilst, in the ConsAC, it ranged from 0.5 to 1.0mm (n=136). Traditional or conservative access cavity preparation in extracted mandibular molars did not influence the remaining dentine thickness in the coronal third of mesial canals enlarged with thermomechanically treated nickel-titanium reciprocating instruments.

To compare the percentage of gutta-percha, sealer and voids and the influence of isthmuses in mesial root canals of mandibular molars filled with different techniques. Canals in 60 mesial roots of mandibular first molars were prepared with ProTaper instruments to size F2 (size 25, 0.08 taper) and filled using a single-cone, lateral compaction, System B or Thermafil techniques. An epoxy resin sealer was labelled with Rhodamine-B dye to allow analysis under a confocal microscope. The percentage of gutta-percha, sealer and area of voids was calculated at 2, 4 and 6 mm from the apex, using Image Tool 3.0 software. Statistical analysis was performed using nonparametric Kruskal-Wallis and Dunn tests (P < 0.05). The influence of isthmuses on the presence or absence of voids was evaluated using the Fisher test. At the 2 mm level, the percentage of gutta-percha, sealer and voids was similar amongst the System B, lateral compaction and single-cone techniques. The single-cone technique revealed significantly less gutta-percha, more sealer and voids in comparison with the Thermafil technique at the 2 and 4 mm level (P < 0.05). The analysis of all sections (2, 4 and 6 mm) revealed that more gutta-percha and less sealer and voids were found in root canals filled with Thermafil and System B techniques (P < 0.05). The Fisher test revealed that the presence of isthmuses increased the occurrence of voids in the lateral compaction group only (P < 0.05). Gutta-percha, sealer filled area and voids were dependent on the canal-filling technique. The presence of isthmuses may influence the quality of root filling.

Effects of Instrumentation and NaOCl Concentration on Microbial DNA Load Employing a Sonic Activation Device.

Comparison of root canal preparation using different automated devices and hand instrumentation

The effects of Er:YAG laser irradiation on the periodontal region during root canal preparation were evaluated using rats histopathologically. The effects on periodontal tissues along the root surface and apical area during root canal preparation using Er:YAG laser irradiation in vivo are not clear. One hundred mesial root canals of mandibular first molars in rats were divided into four groups. In three of these groups, root canals were irradiated using an Er:YAG laser at 2 Hz and 34, 68, and 102 mJ/pulse for 30 sec. Non-irradiated canals served as controls. The effects of laser irradiation on the periodontal tissues along the root surface and apical area were evaluated at 0, 2 days, 1, 2, and 4 weeks after irradiation under light microscopy. At 4 weeks after treatment, no inflammation or resorption was observed in any cases in the control or 34 mJ/pulse-irradiated groups. However, moderate to severe inflammation with resorption of root surface was observed in four of five cases (80%) in the 102 mJ/pulse-irradiated group, which was significantly different from the control (p < 0.01). These results suggest that the effects on periodontal tissues during root canal preparation by Er:YAG laser irradiation after pulpectomy are minimal, if appropriate parameters are selected, and this is a potential therapy for human root canals of teeth.