Short protocol for antioxidant therapy in men with pathospermia

The state of reproductive system of 15 dogs of German shepherd breed at the age of 1.5-5 according to the indexes of quality of sperm (by its volume, activity, concentration and amount of dead spermatozoa) and bioresonance testing by a diagnostic complex «ПАРКЕС-Д», the principle of action of which is based on determination of conductivity of biologically active points at bringing in an electromagnetic contour micro resonance contours were investigated. On the final stage of research a comparison of the indicated methods of research was made. It was determined that unlike animals of control group, dogs of experimental group had a volume of ejaculate on average in 3 times (p<0,001) less, concentration of spermatozoa in sperm in 2,05 times (p<0,001) less, capacity of direct motion by 37,0 % (p<0,001) less, and the amount of dead spermatozoa was by 40 % (p<0,001) more. The survival of spermatozoa of dogs of experimental group at the temperature of 5°C did not exceed 14–20 hours and was less compared to the dogs of control group. Consequently, the indexes of sperm of dogs of experimental group characterize the low functional state of their reproductive function. Using the complex «ПАРКЕС-Д» it was determined that the volume of conductivity in bioactive points ranged from 24 to 43 c.u. and reliable bioresonance was within the limits of 8–20 c.u. During the research of the phenomenon of bioresonance in 19 dogs with the use of nozode in relation to the state of the reproductive system bioresonance was observed in 13 dogs. Testing with nozode in relation to the decreased functional state of reproductive function of dogs in 6 dogs of experimental group bioresonance was observed within the limits of 10-20 c.u. and in animals of the control group – 0-4 c.u. The conducted regressive analysis of index of bioresonance of the state of the reproductive system in dogs indicated that the value of index of bioresonance in animals is connected to the volume of ejaculate (b=-0,859; p < 0,01), concentration of spermatozoa (b=-11,5; p < 0,01), amount of dead spermatozoa (b=0,451; p < 0,01) and their survival at the temperature of 5°C (b=-0,288; p < 0,01). Consequently, the results of research of the functional state of the reproductive system of dogs according to different methods accord by 89,5 % that allows to use programmatic diagnostic complex «ПАРКЕС-Д» for determination of the functional state of the reproductive system in dogs.

Evaluating Spermatogenesis Using Semen: The Biology of Emission Tells Why Reporting Total Sperm per Sample Is Important, and Why Reporting Only Number of Sperm per Milliliter Is Irrational

Objective. Timely diagnosis of adenomyosis, identification of distinctive and similar signs in comparison with other benign diseases of the uterus, as well as the study of the features of reproductive disorders in this disease. Material and methods. 4 groups of patients were examined: the control group consisted of 50 practically healthy women; the 1st group consisted of 165 patients without adenomyosis, with impaired reproductive function and with other benign uterine diseases; the 2nd group consisted of 19 patients with adenomyosis and impaired reproductive function; the 3rd group consisted of 16 patients with impaired reproductive function — functions in which adenomyosis was combined with one of the benign diseases of the uterus (uterine fibroids, endometrial hyperplasia, endometrial polyps). Results. With adenomyosis, the frequency of reproductive function disorders increased with increasing age of the patients. Menstrual cycle disorders most often appeared in the form of menometrorrhagia and dysmenorrhea. In group 2, secondary infertility was more common than in the other groups. In the group with isolated adenomyosis, the levels of estradiol, luteinizing hormone, were higher, and the level of progesterone in the blood was lower than in the other groups. Blood prolactin levels were higher in the groups with adenomyosis (groups 2 and 3) compared with those in the control and 1st groups. Changes in uterine artery hemodynamics were observed between the groups. In the 2nd group with adenomyosis, the systolic ratio, resistance index, and relaxation index were higher than in the other groups. Conclusion. Timely assessment and diagnosis of clinical signs of adenomyosis, as well as identification of differences in laboratory and instrumental examination parameters from those in other benign uterine diseases, form a pathogenetically based approach to the realization of reproductive function in patients with adenomyosis.

The aim of the study was to estimate genetic characteristics for important qualitative and quantitative parameters of Czech Fleckvieh bulls’ ejaculate and to define factors which influence these parameters. a total number of 2,929 entries about samples from 163 bulls of a selected artificial insemination centre were used for calculation. the analysed ejaculate characteristics were: volume of ejaculate, sperm concentration, total sperm count, native sperm activity and activity after thawing. In all evaluated semen characteristics, the age of individual, coefficient of inbreeding, interval between colection and year of colection were statistically significant (P &lt; 0.05). In the case of season of colection, statistically significance (P &lt; 0.01) was proved in all monitored semen parameters, except concetration of spermatozoa where statistically significant difference was not found (P &gt; 0.05). Estimated coefficients of heritability correspond to the values standardly specified for reproductive parameters (h2= 0.003 – 0.14), except for the volume of ejaculate (h2= 0.31). the highest value of genetic correlation was recorded between the volume of ejaculate and the total sperm count (rG= 0.9), on the contrary, the lowest value was between the volume of ejaculate and the sperm concentration (rG= 0.32).

The assessment of reproduction characteristics of an Algerian local sheep in a semi-arid zone was the objective of this study.Twenty rams of Ouled Djellal breed were used.The parameters analyzed were testosterone rate, testicular diameter, ejaculate volume, sperm concentration, mass motility, and percentage of live and dead spermatozoids (SPZ).The results obtained showed that the mean monthly testosterone rate was high throughout the year (4 ng/mL), increased in spring, peaked in summer (July) (4.9 ng/mL), and decreased in autumn and winter to reach a minimum value (January) of 1.9 ng/mL.Similarly, testicular diameter, ejaculate volume, sperm concentration and count showed high levels in spring and summer and decreased in autumn and winter.The maximum and minimum values were respectively, 7.09 cm, 1.55 mL, 4 billion/mL, 3.5, 79.37%, 21% and 5.46 cm, 0.80 mL, 2.50 billion SPZ/mL, 2.81, 77.36%, and 20.42%.Significant effects of season on serum testosterone levels, testicular conformation, ejaculate volume, and concentration were recorded in this study.Indeed, the rates of these parameters increased significantly (P<0.05) during spring/summer and decreased during autumn/winter except for motility, and the percentage of live and dead sperm.Testicular conformation was positively correlated with testosterone concentration, ejaculate volume, and semen concentration.To conclude, Ouled Djellal breed seemed not to be very sensitive to the photoperiod and little affected by high temperature.

Research in brief

Protective role of ascorbic acid to enhance reproductive performance of male rabbits treated with stannous chloride

Aim: Present investigation was carried out to study the physical characteristics of Kankrej bulls semen by evaluation of various semen parameters from neat semen and at various stages of semen preservation. Materials and Methods: A total of 60 ejaculates, 10 each from 6 mature Kankrej bulls, once in a week for 10 weeks, were collected and analyzed for various semen attributes. Result: The mean values for different seminal attributes were: ejaculate volume 4.84 &#177; 0.01 ml, pH 6.88 &#177; 0.01, mass motility 3.72 &#177; 0.02, sperm concentration 1253.83 &#177; 14.68 million / ml, individual motility 86.15 &#177; 0.30 per cent, live sperm count 90.58 &#177; 0.20 per cent, abnormal sperm count 4.24 &#177; 0.03 per cent and acrosomal integrity 81.17 &#177; 0.11 per cent. The colour of the Kankrej bull semen under the investigation was creamy white. Mean values of ejaculate volume, sperm concentration, live sperm count and acrosomal integrity of semen differed significantly (P < 0.05) among the bulls under investigation. The ejaculate volume was positively correlated with mass motility (+ 0.392) and sperm concentration (+ 0.385) and inversely proportional to the mass motility whereas mass motility positively correlated with volume (+0.392), individual sperm motility (+0.329) and live sperm count (+0.527). Conclusion: It can be concluded that the volume, pH, mass motility and sperm concentration of Kankrej bull semen were well comparable with other breeds of Indian cattle, however higher individual motility, live sperm count, acrosomal integrity and lower abnormal sperm count were recorded in the Kankrej bull semen. [Vet World 2013; 6(7.000): 405-408]

A total of 18 hybrid strain Martini male rabbits were randomly divided into three experimental groups. The animals in the first and second groups drank water with 0.1 and 0.5 g/l of lycopene addition (B and C groups, respectively), while water without any supplement was administered to the control group rabbits (A group) for 8 weeks. Semen was collected from 18 bucks (6 animals/group) for 5 consecutive weeks. Ejaculate volume was determined by graduated test tube and sperm concentration was calculated by Neubauer chamber. Sperm motility was evaluated subjectively by a phase contrast microscope and forward progressive motility (FPM) scored 1÷4 (low-high). Sperm viability was assessed by nigrosin/eosin (N/E) staining procedure. At the end of the semen evaluation period, 20 females per group were inseminated on day 11 post partum with a pool of semen collected from each experimental group. Data showed that the highest level of lycopene (C group) resulted in a significantly greater volume of ejaculate and total number of sperm than in the control group (0.98 vs. 0.78 ml and 364 vs. 227, P&lt;0.05), while sperm concentration was not affected. Lycopene addition did not significantly affect the sperm quality of fresh semen, but influenced the semen traits during storage (24h at 5°C). In particular, the motility, FPM and viability of the C group were significantly higher (P&lt;0.05) than those found in the A and B groups. No significant differences were found in reproductive performance (P&gt;0.05). This study shows the positive effects of lycopene supplementation on semen production and refrigerated semen characteristics. Lycopene supplementation of drinking water could have interesting applications in field conditions, although further research into the role of this antioxidant in animal production is needed.

ABSTRACT: The aim of this study was to evaluate the effects of inclusion aqueous extract of Tribulus genuses (ETT) with selenium on semen quality and physiological response of mature buck rabbits during summer season. Thirty six of mature buck rabbits and forty-eight does of Californian breed were randomly divided into six equal treatment groups (6 buck + 8 does per each). The first treatment group was fed on basal diet (unsupplied group) and served as control. The other five treatments were fed the same basal diet and orally supplied with 0.4 mg Se/L (Se), 50 mg ETT/kg BW (ETT1), 100 mg/kg BW (ETT2), 0.4 mg Se/L+ 50 mg ETT/kg BW (ETT1+Se) and 0.4 mg Se/L+100 mg ETT/kg BW (ETT2+Se), respectively. The results indicated that the different supplied groups had a significantly higher follicle stimulating (FSH), luteinizing (LH), testosterone, progesterone and estrogen hormones levels than the control group. The groups supplemented with of ETT and Se showed high levels of FSH, LH, FT and E217β than another groups. However, no significant differences were found among all supplemented groups regarding blood biochemical parameters. Administrations of ETT and Se significantly increased plasma total antioxidant capacity and immunoglobulin M levels. On the other hand, the level of malondialdehyde was significantly decreased for the treated groups compared with the control group. Also, ejaculate volume, sperm motility, sperm concentration, cell integrity, fertility rate and litter size at birth were significant increases, while reaction time, abnormal sperm and dead sperm were significantly decreased. The groups ETT1+Se and ETT2+Se recorded height rate of fertility and increase number of litter size at birth. Treated groups showed statistically heaviest final body weight, body weight gain and lowest feed conversion compared with the control group. Conclusively, extract Tribulus Terrestris (al-Hasakah) plantwith selenium led to improve the activity of sex hormones, oxidative status and immunity, and the additives improved the quality of semen and increased fertility and Litter size at birth abdominal size at birth during summer condition. Key word:

IntroductionGastric acid-related symptoms are highly prevalent in the general population (21–40%), and more than 11% of individuals use medication for the treatment of these symptoms. The uptake of micronutrients is dependent on the gastrointestinal potential of hydrogen (pH).ObjectiveWe hypothesized that medication affecting gastrointestinal pH reduces the availability of B vitamins, thereby deranging one-carbon metabolism and detrimentally affecting spermatogenesis.MethodsThis explorative nested case–control study in men of subfertile couples investigated associations between medication used for gastric acid-related symptoms and semen parameters. We included 40 men using medication for gastric acid-related symptoms and 843 men not using medication. Semen analyses were performed between 70 days before and 21 days after the visit.ResultsThe use of medication was associated with a twofold higher risk of a low total motile sperm count [TMSC <1 × 106, odds ratio (OR) 2.090, p = 0.049] and negatively with sperm concentration (β −0.320, p = 0.028). Red blood cell folate was positively associated with TMSC (β 0.257, p = 0.026), sperm count (β 1.679, p = 0.013) and ejaculate volume (β 0.120, p = 0.023), and total homocysteine (tHcy) was negatively associated with sperm count (β −0.077, p = 0.021).ConclusionHere we delineate associations between the use of medication for gastric acid-related symptoms and poor semen quality in men of subfertile couples. The use of medication for gastric acid-related symptoms is associated with a twofold higher risk of a low TMSC and a decreased sperm concentration. Although these findings warrant further research on causality, the associations between folate, tHcy and semen quality emphasize the importance of preconception counselling in male subfertility.Electronic supplementary materialThe online version of this article (doi:10.1007/s40264-016-0488-8) contains supplementary material, which is available to authorized users.

Influence of increasing body mass index on semen and reproductive hormonal parameters in a multi-institutional cohort of subfertile men

For the first time in our country for the purposes of artificial insemination, semen was regularly obtained from trained boars of the East Balkan Swine breed. The aim of the study was to determine ejaculate volume, concentration, number of doses for insemination. The study covered the period from October 2023 to May 2024, and seventy-seven ejaculates from ten boars were examined. Analysis revealed an average volume of the ejaculate of 161.417 ± 5.281 ml, with the minimum and maximum values being within very wide range – 40 ÷ 300 ml. Sperm concentration and total concentration were, respectively, 0.575 x109/ml ± 0.012, and 90.89 x109/ml ± 2.675. The reported mean number of doses received from one ejaculate was 10.618 ± 0.43. The boar as a fixed factor had a significant effect on the studied parameters of the sperm (p&lt;0.001). The month of semen collection appeared to be a significant source of variation in the total concentration and number of insemination doses. The boar, as a factor within the year, significantly influenced ejaculate volume and concentration (p&lt;0.001), as well as total concentration (p&lt;0.05). In the analysis of monthly dynamics of the main semen traits, lower values were observed for most traits during the first two months of the experiment. Despite the relatively aggressive temperament of animals from primitive breeds like the East Balkan Swine breed, obtaining semen manually was entirely feasible and applicable with appropriate training and adaptation of animals. Keywords: East Balkan Swine, boars, sperm concentration, ejaculate volume

Electroejaculation in the Chinchilla ( Chinchilla lanigera): effects of anesthesia on seminal characteristics

Seventy-two mature New Zealand White (NZW) rabbit's bucks were randomly and equally divided into eight groups each of nine bucks to evaluate the impact each of rocket seeds (RS), carrot seeds (CS), or bay laurel leaves (BLL) and their mixtures on semen quality, biochemical components and the physiological response of NZW rabbits bucks during hot summer conditions. The study started in June and lasted for 8 weeks. Eight experimental diets were formulated such that diet (D1) free additives as a control group. Diets 2, 3 and 4 contained 1.0% RS, 1.0% CS and 1.0% BLL, respectively. Diet 5, 6 and 7 contained a mix of 0.5% RS+0.5% CS, 0.5% CS+0.5% BLL and 0.5% RS+0.5% BLL, respectively, while diet 8 contained 0.33% RS+0.33% CS+0.33% BLL. Insignificant effect on buck's body weight, however, feed intake was significantly increased as compared to bucks group given the control diet. Supplementation with RS, CS, BLL, and their mixtures caused significant improvement in ejaculate volume, individual motility, total motile sperm, sperm concentration, live sperm%, total functional sperm fraction, total sperm output, and decreased abnormal sperm%. Seminal plasma initial fructose and globulin increased significantly with RS, CS, BLL, and their mixtures treatments. Seminal plasma alkaline phosphatase (ALP), alanine aminotransferase (ALT), and aspartate aminotransferase (AST) appeared reduction significantly with the RS, CS, BLL and their mixtures treatments. Seminal plasma total antioxidant capacity (TAC) increased, while, malondialdehyde (MDA) levels significantly decreased in all supplemented groups compared with the control bucks' value. Most of the supplemented groups showed a significant decrease in blood plasma glucose, cholesterol, triglycerides, and total lipids, low-density lipoproteins, AST and ALT as compared to the control group. However, blood plasma total protein and albumin were increased in comparison with the control group. Blood plasma TAC significantly increased due to the addition of RS, CS, BLL, and their mixtures as compared to the control group, but MDA levels decreased.