Silver Nanoparticles Production with Probiotic Bacteria

Production of silver nanoparticles by laser ablation in open air

Green synthesis of nanoparticles is a more eco-friendly, cost-effective, and sustainable alternative to physical and chemical approaches of synthesis. Using medicinal herbs like Phyllanthus emblica can improve the therapeutic potential of manufactured nanoparticles. While numerous plant extracts have been utilized to synthesize silver nanoparticles, few investigations have focused on Phyllanthus emblica leaf extracts. The primary goal of this study was to produce silver nanoparticles from Phyllanthus emblica leaf extract and assess their structural characteristics and antibacterial activity. Silver Nitrate is used as metal precursor and Phyllanthus emblica is used as reducing and stabilizing agent. After a visual colour shift from yellow to brown, the formation was verified by Ultraviolet-Visible spectroscopy. It was then further described using Fourier transform infrared spectroscopy to determine the functional group's involvement and the Agar well diffusion method to assess its antibacterial activity against both Gram-positive and Gram-negative bacterial strains. A far-off Surface Plasmon Resonance peak at about 440 nm was visible in the Ultraviolet-Visible Spectroscopy, indicating the production of Silver Nanoparticles. Hydroxyl (O–H), carbonyl or aromatic (C=O or C=C) and metal ligands (Ag–O or Ag–N) functional groups were detected by Fourier Transform Infrared Radiation analysis, suggesting their role in the production and stabilization of silver nanoparticles. The produced silver nanoparticle demonstrated significant antibacterial action, particularly against bacteria that are Gram-negative. These results demonstrate the potential of silver nanoparticles mediated by Phyllanthus emblica as strong antibacterial agents. The objective of this study was to synthesize silver nanoparticles using Phyllanthus emblica leaf extract, characterize them using standard techniques, and evaluate their antibacterial potential against selected bacterial strains.

In this nanoregime attempts to bring forth nanoparticles and nanomaterials are myriads, with there interesting and demanding applications in almost every field. Today the field of nanoscience has bloomed with the confluence of nanotechnology with material science, biology, biotechnology and medicine and the need for nanotechnology will only increase as miniaturization becomes extremely important in various arrays of life. Since time immemorial silver nanoparticles have been extensively used for hygienic and healing purposes, and even until most recently, it has indispensible vital role especially in the biomedical arena. Thus in an attempt to generate silver nanoparticles employing green, environmentally benign route, we have designed to converge mythology with technology, with the mystical production of silver nanoparticles, enabled by the blueberry beads of the plant Elaeocarpus granitrus Roxb., the Rudraksha. This non-degradable bead does not disintegrate, but retains the potentiality, even after unlimited production of silver nanoparticles, assisting infinite times. The extremely cost-efficient nanoparticles thus developed in a superiorly efficient manner were characterized through different techniques; like UV/visible spectroscopy, PL spectroscopy, transmission electron microscopy, energy dispersive X-ray analysis and nanoparticle size analysis.

Objectives: The aim of this study is to synthesize silver nanoparticles using the algal extract of Padina tetrastromatica and evaluate its antifungal activity against pathogenic fungus isolated from clinical samples.Results: Formation of brown color at 15 minutes indicates the production of silver nanoparticles by the extract of brown algae P. tetrastromatica. Surface plasmon resonance band was centered at 440 nm which was observed by UV-vis spectrophotometer. SEM image revealed spherical and cubical nanoparticles with high agglomeration, and energy-dispersive X-ray illustrates elemental components of silver formed at 3 keV. TEM shows spherical, truncated, and ellipsoidal nanoparticles and also it evidences the algae compounds that are capped with nanoparticles. SAED pattern proved four diffraction face-centered cubic rings at (111), (200), (220), and (311) which indicates the crystalline nature of nanoparticles. Silver nanoparticles show high inhibition activity against Fusarium sp, Aspergillus niger, Candida albicans, Aspergillus fumigatus, and Aspergillus flavus at different concentrations. P. tetrastromatica-mediated synthesis of silver nanoparticles shows rapid and eco-friendly silver ion reduction process.Methods: Dried algal biomass was used to prepare the pure algal extract and added with 1 mM AgNO , and the color change was noted and recorded by ultraviolet (UV)-vis spectrophotometer. The morphological characteristics were analyzed by scanning electron microscopy (SEM) and transmission electron microscopy (TEM). Crystalline structure was analyzed by SAED pattern. Antifungal activity was performed by agar well diffusion method against various pathogenic fungi.Conclusion: Therefore, this present study elucidates that algae-mediated synthesized silver nanoparticles have antifungal activity against pathogenic fungi, so it can be developed as a novel medicine for human welfare in biomedical applications in the near future.Keywords: Padina tetrastromatica, Silver nanoparticles, Transmission electron microscopy, Antifungal activity, Green synthesis.

In the present study, synthesis, characterization, and the antibacterial activity of silver nanoparticles from native isolate of Corynebacterium glutamicum has been reported. Silver nanoparticles were synthesized by challenging the dried biomass of C. glutamicum with aqueous diamine silver ([Ag (NH3)2]+) containing 1 mM AgNO3. Synthesized silver nanoparticles (AgNPs) were characterized by ultraviolet–visible spectroscopy and energy-dispersive X-ray (EDX) spectroscopy analysis. Morphological study of silver nanoparticles was carried out using transmission electron microscopy (TEM) and scanning electron microscope (SEM). The spherical morphology of silver nanoparticles was confirmed from SEM image. The TEM image showed the average particle size of silver nanoparticles was about 15 nm. Silver nanoparticles synthesized from C. glutamicum were found to have enhanced antimicrobial activity against selected pathogenic strains. Silver nanoparticles from pure strains of Corynebacterium species was done by many investigators, but as per the present literature, this is the first report on the production of silver nanoparticles using a native strain of Corynebacterium.

AimsThe study aimed to explore the sustainable synthesis of metal nanoparticles using a green and eco-friendly resource. Specifically, it investigated the utilization of Cannabis sativa waste extract for the production of gold and silver nanoparticles, focusing on their antimicrobial activity against gram-negative bacteria, particularly Pseudomonas aeruginosa strains, which are significant in nosocomial infections.MethodsCannabis sativa waste extract was employed to synthesize gold and silver nanoparticles through a green synthesis approach. The produced nanoparticles were characterized using transmission electron microscopy (TEM), atomic absorption spectrometry (AAS), X-ray diffraction (XRD), and Fourier transform infrared spectroscopy (FTIR). The antimicrobial efficacy of the synthesized nanoparticles was assessed through their minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC), and minimal biofilm inhibitory concentration (MBIC) against Pseudomonas aeruginosa, utilizing a microcultivation device, solid medium cultivation, and a metabolic activity assay in a polystyrene microtiter plate, respectively.ResultsThe TEM analysis revealed the size and morphology of the nanoparticles, while AAS confirmed their concentration. XRD provided insights into the crystalline structure, and FTIR analysis identified the molecular structure of the nanoparticle’s stabilizing layer. The synthesized nanoparticles showed significant antimicrobial activity against Pseudomonas aeruginosa, with determined MIC, MBC, and MBIC values of produced silver nanoparticles, showcasing their potential as effective antimicrobial agents.ConclusionsThis study successfully demonstrated the synthesis of silver and gold nanoparticles using Cannabis sativa waste extract and highlighted their potent antimicrobial properties. It underscores the potential of utilizing plant waste extracts in sustainable nanomaterial synthesis and contributes to the fields of green nanotechnology and waste valorization within the circular economy. The findings also offer valuable insights into developing natural waste source-based antimicrobial agents.

An important aspect of food technology is that vitamin compounds can be used for a variety of purposes, such as developing methods to enhance the nutritional value of foods. This paper discusses the synthesis and properties of β-cyclodextrin (β-CD)-functionalized silver nanoparticles, and the use of the resulting β-CD-AgNP inclusion complex when loading vitamin D3 (cholecalciferol, VD3) molecules. β-Cyclodextrin was used as a reducing agent and a stabilizer in the production of silver nanoparticles. The preparation of VD3-β-CD-AgNP nanocompositions was confirmed by UV spectroscopy, transmission electron microscopy, and X-ray diffraction spectroscopy. Scanning electron microscopy (SEM) and transmission electron microscopy (TEM) revealed that the resulting β-CD-VD3-AgNP nanocomposite was well dispersed with particle sizes ranging from 6 to 15 nm. 1H-, 13C-NMR and FTIR spectroscopy showed the reduction of silver ions and the formation of β-CD-encapsulated AgNPs. The kinetic parameters of the thermal decomposition reaction of the VD3-β-CD-AgNP nanocomposition have been determined under nonisothermal conditions that ensure the preservation of the kinetic triplet and a more accurate description of the process. The nanocomposition of VD3 with silver nanoparticles demonstrated antibacterial activity against the used bacteria.

In this paper we report a facile route for synthesis of myristic acid (C13H27COOH) capped silver nanoparticles under ambient reaction conditions. AgNO3 has been reduced by NaBH4 in the presence of myristic acid in a water-methanol medium at room temperature. pH-induced optical properties of silver nanoparticle were investigated. Particles synthesized were characterized by ultraviolet–visible spectroscopy, transmission electron microscopy (TEM), X-ray diffraction (XRD) and Fourier transform infrared (FTIR) spectroscopy. The myristic acid capped silver nanoparticles were found to be 3–7 nm in diameter and exhibit remarkable stability for more than six months. This synthetic route would be advantageous over previous reports of carboxylate stabilized silver nanoparticles using extreme reaction conditions. Noteworthy of the advantages of this synthetic method includes its production of silver nanoparticles in an aqueous medium at room temperature under no inert atmosphere, making the synthesis more environment...

Silver nanoparticles are potent antimicrobials and could be used as a promising alternative of conventional antibiotics. The aim of this study was to isolate bacteria from soil that have ability to produce AgNPs by secondary metabolite activity and their elucidation against human pathogens. These strains Escherichia coli, Exiguobacterium aurantiacumm, and Brevundimonas diminuta with NCBI accession number MF754138, MF754139, and MF754140 respectively were grown for secondary metabolite production. The nanoparticles were confirmed and characterized by UV-Vis spectroscopy and transmission electron microscopy. The optimization study was also carried out to obtain the maximum production of silver nanoparticles. Three parameters, temperature, pH, and AgNO3 concentration, were used to optimize the production of silver nanoparticles. Antimicrobial potential of these nanoparticles was addressed on the Muller-Hinton Agar, and their zones of inhibitions were measured. TEM analysis revealed the size and shape of the silver nanoparticles. All types of AgNPs were spherical in shape; their size range is from 5 to 50nm. The findings of optimization study showed the maximum production of silver nanoparticles at the pH9, temperature 37°C, and 1mM AgNO3 concentration. All the strains exhibited the great potential as antimicrobial agents against MRSA and several other MDR bacteria with minimum 10mm to maximum 28mm zone of inhibition. It was concluded that the present study is an eco-friendly approach for the synthesis of AgNPs that will be beneficial to control the nosocomial infections triggered by MRSA and other human pathogens.

This study reports screening of 102 Iranian medicinal plant species belonging to 49 families for the biosynthesis of colloidal silver nanoparticles (AgNPs). Among tested plants, 37 species in 19 plant families are new bio-resources in this regard. Water extracts of macerated plant parts are used in this survey. Six of the bioactive plants are selected for further analysis. They produced amorphous spherical 40-70 nm AgNPs. Biosynthesised colloidal AgNPs are characterised by UV-visible spectroscopy, transmission electron microscopy and scanning electron microscopy. Families of Lamiaceae (8 out of 12), Leguminosae (7 out of 9), Liliaceae (4 out of 7) contained most bio active species. No correlation noticed between bioactivity and pH of plant water-extracts. The simple procedure used in this study may form a platform for mass production of environmentally safe and eco-friendly green production of these multipurpose AgNPs in future; however, optimisation protocols need to be well documented.

In this work the effects of the pressure between 1-150 Bar on pulsed laser ablation in liquids (PLAL) during the production of silver nanoparticles (AgNPs) in water was investigated. The produced NPs are the results of two different well-known stages which are the plasma and the bubble evolution occurring until the generated material is released into the solution. The main aim of this work is to show which roles is played by the variation of water pressure on the laser induced plasma and the cavitation bubble dynamics during the NPs formation. Their implication on the comprehension of the as-produced NPs formation mechanisms is treated. The typical timescales of the different stages occurring in water at different pressures have been studied by optical emission spectroscopy (OES), imaging and shadowgraph experiments. Finally surface plasmon resonance (SPR) spectroscopy, transmission electron microscopy (TEM), dynamic light scattering (DLS) and scanning electron microscopy (SEM) for characterization of the material released in solution, have been used.

Utilization of aqueous broccoli florets extract for green synthesis and characterization of silver nanoparticles, with potential biological applications

Synthesis characterization and application of butyl acrylate mediated eco-friendly silver nanoparticles using ultrasonic radiation

Compared to traditional production method (chemical method), biological synthesis of nanoparticles tend to be cheaper since no chemical reducing agents are required, secondary metabolites easily reduced metal ions to nanoparticles in simple steps. The present study compared the antimicrobial activities of both chemically and biologically produced nanoparticles. Aqueous extract of Pittosporum senacia, an endemic medicinal plant, was used to produce silver nanoparticle. Sodium citrate was used as reducing agent in the chemical production of silver nanoparticle. Produced silver nanoparticles were then characterised using UV-Vis spectroscopy. Absorption peak were produced at 400 nm by both type of nanoparticles indicating their spherical nature. Peak at 600 nm was also observed for the biosynthesized nanoparticle denoting other shapes of nanoparticles or possible aggregation. Phytochemical test revealed the presence of saponins, carbohydrates and phenols that were responsible for the reduction and stabilisation of biosynthesized silver nanoparticle. The antimicrobial activities of both type of synthesized nanoparticle were tested against both Gram positive and Gram negative bacteria and a fungus. Excellent antimicrobial activities were recorded against Escherichia coli using both chemically and biologically synthesized silver nanoparticles, reporting inhibition zone of 20.0 ± 0.0 mm and 21.67 ± 1.15 mm respectively. No synergistic interaction was found between biologically synthesized nanoparticles and antibiotic, chloramphenicol. This study demonstrated that there was no significant difference in the antimicrobial properties of both type of nanoparticle and Pittosporum senacia leaves can be effectively used to synthesize silver nanoparticles.

Nanotechnology is a rapidly growing field due to its unique functionality and a wide range of applications. Nanomedicine explores the possibilities of applying the knowledge and tools of nanotechnology for the prevention, treatment, diagnosis and control of disease. In this regard, silver nanoparticles with diameters ranging from 1 to 100 nm are considered most important due to their unique properties, ability to form diverse nanostructures, their extraordinary range of bactericidal and anticancer properties, wound healing and other therapeutic abilities and their cost-effectiveness in production. The current paper reviews various types of physical, chemical and biological methods used in the production of silver nanoparticles. It also describes approaches employing silver nanoparticles as antimicrobial and antibiofilm agents, as antitumour agents, in dentistry and dental implants, as promoters of bone healing, in cardiovascular implants and as promoters of wound healing. The paper also explores the mechanism of action, synthesis methods and morphological characterisation of silver nanoparticles to examine their role in medical treatments and disease management.