Smartphone-based Colorimetric Analysis for the Detection of H2O2 Using a ¼PAD

Abstract

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Paper-based microfluidic analytical devices (¼PAD) have great potential for use in a variety of areas, from environmental monitoring to clinical and bedside testing. Such devices are generally designed to be disposable, cost effective, practical and user-friendly. In this study, a smartphone based ¼PAD was developed which can be used in high sensitive quantitative analysis of H2O2 in transparent liquids such as water. Solid ink technology is used in the production of ¼PADs. The H2O2 concentration dependent color change (colorimetric) used in the measurement was catalyzed by horseradish peroxidase (HRP). In addition, the effect of chitosan on the measurement sensitivity was investigated. The resulting color changes were recorded in a dark environment with a special apparatus integrated smartphone camera and the color changes were digitized by an algorithm developed in MATLAB. The results showed that the produced ¼PADs can successfully be used to quantitatively analyze H2O2 between X and Y, and the chitosan significantly increases the sensitivity.