STURGEON DISEASES IN AQUACULTURE

A wide range of pets, including aquarium and terrarium species, as well as associated organisms, are available via numerous formal, informal, poorly-regulated, or even illegal outlets. Once present in a country, escapes and even deliberate releases are likely to occur. If freed pets become established in the area, they frequently turn invasive. In the Caribbean, internet trade easily eludes proper procedures, and the sheer volume of exotic pets kept, traded, or released in Florida represents a threatening source for invasive alien species (IAS) for the Wider Caribbean Region (WCR). The negative impact can be via predation, competition, habitat alteration, hybridization, vectoring of animal pathogens or parasites (aggravated by shared facilities in trade hubs), zoonoses, and interference with human activity or infrastructure. These mechanisms have led to extinctions of previously rare endemic species in the WCR and continue to threaten others. Examples of IAS originating from the pet trade span across terrestrial, marine, and freshwater species, including plants and live feed for the aquarium trade, as well as bird seeds. Aquatic organisms and increasing outbreaks of zoonoses are particularly problematic. Only a few international standards address risks of invasions associated with the pet trade and only for specific taxa, that is, the Convention on International Trade in Endangered Species of Wild Fauna and Flora (CITES), the International Plant Protection Convention (IPPC), and the World Organisation for Animal Health (OIE). This gap is now being addressed by an ad hoc Technical Expert Group under the Convention of Biological Diversity (CBD). Existing and incipient preventative tools include pre-importation measures (i.e., importation bans and post-importation measures) that focus on the prevention of release/escape or reproduction of IAS. Their successful deployment invariably involves close collaboration between the government and the private sector, coupled with public involvement and broad-based awareness-raising.

Pathogens of wildlife can have direct impacts on human and livestock health as well as on biodiversity, as causative factors in population declines and extinctions. The World Organization for Animal Health (OIE) seeks to facilitate rapid sharing of information about animal diseases to enable up-to-date risk assessments of translocations of animals and animal products. The OIE also produces manuals of recommended methods to standardize diagnostic testing. Ranaviruses are important amphibian pathogens that may have spread through international trade, and infections became notifiable to OIE in 2009. We surveyed and reviewed published literature for data on sampling, diagnostic testing, and reporting of ranavirus during 2009-14. We also investigated attitudes and awareness of the OIE and its recommendations for best practice. We found that sampling effort is uneven and concentrated in the northern hemisphere. We also identified citizen science projects that have the potential to improve the quantity and quality of data on the incidence of ranavirus infection and the circumstances surrounding disease outbreaks. We found reporting of infection to be inconsistent: reporting was split between the published literature (where it was subject to a 2-yr lag) and the OIE with little overlap, results of negative diagnostic tests were underreported, and scientific researchers lacked awareness of the role of the OIE. Approaches to diagnostic screening were poorly harmonized and heavily reliant on molecular methods. These flaws in the mechanisms of ranavirus detection and reporting hamper the construction of a comprehensive disease information database.

9 - The perspective of the World Organisation for Animal Health

Owing to its large size and ethnic, social, cultural and economic diversity, the Americas' production volume is set to make the region one of the world's leading providers of animal foodstuffs. Animal husbandry, transport and slaughter conditions vary from country to country in response to their differing climatic and geographic characteristics. This article examines the main drivers of animal welfare in the Americas, including the standards of the World Organisation for Animal Health (OIE), legislation, codes of practice and advances in education, training, research and development. It recognises the important roles played by all the various stakeholders in changing perceptions of animal welfare by raising public awareness and promoting communication and cooperation as drivers of overall change in the Americas. Regional and international organisations, public and private-sector bodies, academia and non-governmental organisations have launched a number of initiatives with encouraging results. In 2009, the OIE established the Chile-Uruguay Collaborating Centre for Animal Welfare Research, which is now the OIE Collaborating Centre for Animal Welfare and Livestock Production Systems and has recently incorporated Mexico. The Collaborating Centre works closely with official OIE Delegates and the Focal Points for Animal Welfare of national Veterinary Services. The OIE Regional Animal Welfare Strategy for the Americas was adopted in 2012, under the coordination of the OIE Regional Representation for the Americas, as a guide for developing future policies based on a regional approach. The way to achieve cultural change for improving animal welfare, operator safety and the sector's profitability is through training and knowledge transfer. The results demonstrate that the joint efforts of all institutions and the active role of the Collaborating Centre have been most effective, as have the continuing education programmes implemented by universities.

Curriculum mapping provides a systematic approach for analyzing the conformity of an educational program with a given set of standards. The Chiang Mai University Faculty of Veterinary Medicine and the University of Minnesota College of Veterinary Medicine joined together in an educational twinning project to map their Doctor of Veterinary Medicine curricula against core competencies identified by the World Organisation for Animal Health (OIE) as critically important for Day 1 veterinary graduates to meet the needs for global public good services. Details of curriculum coverage for each specific and advanced competency were collected through a review of syllabi and course descriptions, followed by in-depth interviews of key faculty members. The depth of coverage of each competency was estimated by the tabulating the number of hours assigned. The teaching methods and levels of learning were also captured. While the overall design of the curricula conformed to the OIE Guidelines for Veterinary Education Core Curricula, the mapping process identified variability in the depth and breadth of coverage on individual competencies. Coverage of the Day 1 Specific Competencies was greater early in the curricula. More gaps existed in terms of the Advanced Competencies than the specific core competencies. Discussion of the identified gaps with faculty members led to opportunities for strengthening the curricula by adjustments of individual courses throughout the curricula. Documentation of teaching methods also led to professional development of new pedagogical skills and redesign of the teaching methods for particular subjects.

This study represents a complete comparative analysis of the most widely used African swine fever (ASF) diagnostic techniques in the European Union (EU) using field and experimental samples from animals infected with genotype II ASF virus (ASFV) isolates circulating in Europe. To detect ASFV, three different PCRs were evaluated in parallel using 785 field and experimental samples. The results showed almost perfect agreement between the Universal ProbeLibrary (UPL-PCR) and the real-time (κ = 0.94 [95% confidence interval {CI}, 0.91 to 0.97]) and conventional (κ = 0.88 [95% CI, 0.83 to 0.92]) World Organisation for Animal Health (OIE)-prescribed PCRs. The UPL-PCR had greater diagnostic sensitivity for detecting survivors and allows earlier detection of the disease. Compared to the commercial antigen enzyme-linked immunosorbent assay (ELISA), good-to-moderate agreement (κ = 0.67 [95% CI, 0.58 to 0.76]) was obtained, with a sensitivity of 77.2% in the commercial test. For ASF antibody detection, five serological methods were tested, including three commercial ELISAs, the OIE-ELISA, and the confirmatory immunoperoxidase test (IPT). Greater sensitivity was obtained with the IPT than with the ELISAs, since the IPT was able to detect ASF antibodies at an earlier point in the serological response, when few antibodies are present. The analysis of the exudate tissues from dead wild boars showed that IPT might be a useful serological tool for determining whether or not animals had been exposed to virus infection, regardless of whether antibodies were present. In conclusion, the UPL-PCR in combination with the IPT was the most trustworthy method for detecting ASF during the epidemic outbreaks affecting EU countries in 2014. The use of the most appropriate diagnostic tools is critical when implementing effective control programs.

Summary A nationwide survey in July and August 2007 employed a representative sample of Chinese sturgeon farms from 2002–2006/2007, including data from previous surveys and official statistics dating back to 1998, showing that the development of China’s sturgeon aquaculture has progressed steadily in the past few years. Acipenser baerii and Amur hybrids (Acipenser schrenckii × Huso dauricus) have become the dominant cultured species, accounting for 80% of total production. Production centers moved from the southeastern coast towards the midwestern inland provinces, where cold freshwater resources are available in abundance. The paper presents production trends as well as the price structure for commercial fish/meat and juveniles in the various provinces and also considers import and export data for products, fertilized eggs and fingerlings. Results show that: (i) production of juveniles in hatcheries has risen only slightly during the survey period; (ii) the number of larvae obtained from the wild gradually declines each year; (iii) the price for commercial fish/meat drastically declined between 1998 to 2002, but remained stable from 2002 to 2007; and (iv) total production from the 83 surveyed farms was almost stable from 2002 to 2007. Major problems influencing sustainable development of sturgeon aquaculture were identified, including: (i) insufficient domestic supply of larvae and the ban for commercial use of A. sinensis; (ii) disorganized hybridization; (iii) the potential threat to the genetics of natural stocks in the Yangtze River; (iv) diseases originating from environmental pollution and poor-quality fish feed; (v) limited domestic markets; (vi) low volume of sturgeon export due to lack of intensive processing; and (vii) lack of organizational structure in the industry.

Costs and benefits of freedom from shrimp diseases in the European Union

SummaryAnimal welfare (BA) has been recently included into the Colombian health legislation, following the guidelines of the World Organization for Animal Health (OIE). Implementing BA concepts in the beef chain requires considering ethical issues and regulatory policies. Animal welfare has implications in meat quality and trade. Ignoring BA concepts leads to economic losses related to death of animals, higher weight losses and lower carcass yield. Furthermore, contusions and seizures decrease the commercial value of beef, among others. This paper reviews several BA concepts that were included into Colombian laws, the implication of its adoption, and the requirements for their implementation.Key words: animal welfare, freedom, health law. Resumen El bienestar animal (BA) ha sido integrado recientemente a la legislación sanitaria colombiana de acuerdo a los lineamientos de la Organización Mundial de Salud Animal (OIE). La importancia de su implementación en la cadena cárnica bovina responde a consideraciones éticas, a las exigencias reglamentarias, a sus implicaciones en la calidad de la carne y a las pérdidas económicas relacionadas con la muerte de los animales, pérdida de peso vivo, disminución de los rendimientos de la canal, decomisos por contusiones y disminución del valor comercial de la carne, entre otros. El presente artículo pretende hacer una revisión sobre los conceptos de BA incluidos en la legislación colombiana, las implicaciones de su adopción y los requerimientos para su implementación. Palabras clave: bienestar animal, bovinos de carne, legislación sanitaria. Resumo O bem-estar animal (BA), recentemente incluído na legislação sanitária colombiana, de acordo com as orientações da Organização Mundial de Saúde Animal (OIE). O BA é importante na cadeia de carne bovina porque responde ás considerações éticas, aos requisitos legais e da qualidade da carne. Também influencia nas perdas econômicas, relacionadas com a morte dos animais, perdas de peso, menor rendimento da canal, contusões e convulsões, diminuindo o valor comercial da carne, entre outros. Este artigo visa rever os conceitos do BA incluídos na legislação colombiana, as implicações da adopção e os requisitos para sua implementação. Palavras chave: bem-estar animal, bovinos de corte, o direito a saúde.

The complex and diverse nature of the World Organisation for Animal Health (OIE) region for Asia, the Far East and Oceania presents both challenges and opportunities in implementing improved approaches to animal welfare. Drivers for improvements include social values, culture, religion, political interest, trade, an increasing global awareness of animal welfare issues, an increasing demand for meat and dairy products, the interest of non-governmental organisations, and the mandate given to the OIE to develop science-based standards for animal welfare. The outcomes-based OIE standards can be amended in the light of new scientific knowledge and implemented by countries in a manner best suited to meet their needs. A number of regional initiatives are described, including a regional strategy, examples of national activities, projects run by the OIE Collaborating Centre for Animal Welfare Science and Bioethical Analysis, and trade measures. Although the overall outlook for improvements in the region looks promising, implementation of standards over the longer-term will require ongoing political commitment, resources and cultural change to ensure sustained improvements.

As an effect of increased globalisation, animal diseases, in particular those transmissible to man, have an immediate global economic and social impact. This fact, dramatically illustrated by the current avian influenza epizootic in South-East Asia and Eastern Europe, clearly demonstrates the crucial importance of the national Veterinary Services (VS) for the prevention, early detection and response for the efficient control of animal diseases. Complying with this mission for the VS presupposes the existence of appropriate governance and legislation and of an official system to control their quality and reliability- an obvious weakness in many developing and in transition countries. The World Organisation for Animal Health (OIE) has therefore developed a project aiming at strengthening the VS in those countries facing the greatest animal health threats and to bring them into line with OIE international standards already adopted by the same countries. Based on the evaluation of the VS and subsequent actions at the global, regional and national levels, the project will have a significant beneficial impact on the targeted countries as well as the international community as a whole, not only in the fields of agriculture, food security and production, and food safety, but also for the local and global prevention of emerging and re-emerging diseases of veterinary and public health importance. The project will be implemented in strong collaboration with the Food and Agriculture Organization. The actions proposed must be considered eligible for the concept of International Public Good.

The World Organisation for Animal Health (OIE) represents 182 countries with a focus on animal health, animal welfare and veterinary public health. The OIE has several Collaborating Centres that support the work of the organisation. The Istituto Zooprofilattico Sperimentale dell’Abruzzo e del Molise ‘Giuseppe Caporale’ (IZSAM) is the Secretariat for the OIE Collaborating Centre Network on Veterinary Emergencies (EmVetNet). In April 2020, the IZSAM initiated a COVID-19 Thematic Platform on Animal Welfare. The working group represented the EmVetNet Collaborating Centres, international institutions, veterinary associations, authorities and animal welfare organisations. Lincoln Memorial University College of Veterinary Medicine recruited summer research students whom catalogued over 1,200 animal welfare related reports and provided 64 report narratives for the working group. IZSAM launched the EmVetNet website (https://emvetnet.izs.it) for public and private exchange of information, materials, and guidelines related to veterinary emergencies. The EmVetNet COVID-19 Thematic Platform on Animal Welfare continues to meet to address emerging issues, strengthen the network for future emergencies, and share information with stakeholders including national Veterinary Services responding to the epidemic.

The Siberian sturgeon, Acipenser baerii Brandt, widely distributed throughout all major rivers in Siberia, inhabits the largest range compared to other sturgeon species. Nowadays, the Siberian sturgeon is the main species for sturgeon aquaculture in several countries. Swimming and respiratory characteristics of fish can be modulated, affected, and constrained by morphological, physiological, and environmental variables. Compared with other sturgeon species, Siberian sturgeon exhibits similar behaviors including station-holding under high water velocity. However, this species has some special swimming characteristics, including relatively strong swimming performance and a relative low drag coefficient incurred by their unique body form and scarce bony scutes and a high ability to cope with the lack of ambient oxygen. This chapter will try to summarize and discuss existing knowledge on behavior and kinematics, capacity, metabolism, and functional morphology in swimming performance of this species and to give enlightenment for potential application on sturgeon aquaculture.

Underestimation of zoonoses is exacerbated in low and middle-income countries due mainly to inequalities with serious consequences in healthcare. This is difficult to gauge and reduce the impact of those diseases. Our study focuses on Paraguay, where the livestock industry is one of the major components of the country's economy. Therefore, the rationale of this study was to develop a case study in Paraguay to estimate the dual impact of zoonotic diseases on both the human health and animal health sector and thus determine the societal burden of such diseases. We conducted a systemic review (including a meta-analysis) to assess the burden of zoonoses in Paraguay, including official reports and grey literature of disease incidence and prevalence. We estimated the Disability Adjusted Life Years (DALYs) and Zoonosis Disability Adjusted Life Years (zDALYs) to measure the difference between the current health status and the desired health situation of animals and the Paraguayan population based on 50 zoonotic diseases suggested by the WHO (World Health Organization), OIE (World Organization for Animal Health) and the National Health in Paraguay. The total DALYs represent 19,384 (95% CI: from 15,805 to 29,733), and zDALYs, 62,178 (95% CI: from 48,696 to 77,188). According to the results, the priority pathogens for DALYs are E. coli, Trypanosoma cruzi, Leishmania spp, and Toxoplasma gondii. When we include the additional animal health burden, the most important pathogens are Brucella spp, E. coli, Trypanosoma cruzi, and Fasciola hepatica for zDALYs. This is the first study to integrate DALYs and zDALYs with important clues related to the health status of Paraguay. Through DALYs and zDALYs, our perspective becomes more complete because we consider not only human health but also animal health. This is important for setting priorities in disease control, especially in a society where livestock contribute significantly to the economy and to human well-being.

The optimization of broodstock nutrition remains a critical challenge in sturgeon aquaculture. This study investigated the effects of wheatgrass juice (WGJ) supplementation of sterlet (Acipenser ruthenus) broodstock on reproductive performance, larval quality, hematological profile and oxidative status. Female sterlets were randomly assigned to three groups: control diet (V1), WGJ-supplemented diet (V2, 4 % inclusion), both (V1 and V2) hormonally stimulated and saline solution (V3) without hormonal stimulation. WGJ's chemical composition revealed substantial levels of bioactive compounds, including chlorophyll (a: 0.936 ± 0.01, b: 0.329 ± 0.01 mg/mL fw), carotenoids (0.167 ± 0.01 mg/mL fw), proteins (1.205 ± 0.04 mg PSB/mL fw), flavonoids (7.381 ± 0.31 mg/mL fw), and polyphenols (2.963 ± 0.05 mg/mL fw). WGJ supplementation significantly enhanced reproductive parameters, with V2 showing higher egg production (0.23 ± 0.03 kg vs. 0.13 ± 0.01 kg) and gonadosomatic index (14.48 ± 1.73 vs. 8.05 ± 0.51) compared to V1. Larvae from WGJ-supplemented females exhibited significantly improved length (10.9 ± 0.3 cm vs. 9.8 ± 0.14 cm, p < 0.001) and weight parameters. Analysis of oxidative stress markers revealed tissue-specific modulation of antioxidant systems, with WGJ supplementation reducing malondialdehyde levels across tissues (muscle: 1.061 ± 0.075 vs. 1.888 ± 0.105 nM/mg protein, p < 0.0001) and enhancing antioxidant enzyme activities. Hematological parameters remained within physiological ranges, with reduced neutrophil counts in the WGJ group suggesting potential immunomodulatory effects. These findings establish WGJ as a promising functional feed additive for enhancing reproductive performance in sterlet aquaculture, while maintaining optimal physiological status through improved antioxidant defense mechanisms.