Trace element concentrations in breast cancer patients

Risk and severity of SARS-CoV-2 infection in breast cancer patients undergoing a structured infection screening program at the University and Hospital Trust of Verona

This study was conducted to evaluate the association of serum leptin and hs C-reactive protein with breast cancer. Two groups were included in the study. The first group included 45 newly diagnosed women with breast cancer. The second group included 42 women with benign breast lump as a control group. Blood samples (5 mL) were taken from the patient and the control groups and analyzed for serum leptin and hs C-reactive protein. Serum CA15-3 was also measured in breast cancer patients. The epidermal growth factor receptor 2 (HER2/neu), estrogen and progesterone receptors were determined in breast cancer patients by using immune-chemical method. Serum leptin was significantly higher (p ≤ 0.05) in breast cancer patients than that in the control group; however, no significant difference was noticed between the two groups for serum hs C-reactive protein. No significant difference was noticed between HER2/neu positive or negative in breast cancer patients for serum leptin or hs C-reactive protein. However, serum CA15-3 in HER2/neu positive patients was significantly higher (p ≤ 0.05) than that in HER2/neu negative patients. No significant difference was noticed between positive and negative estrogen breast cancer patients for serum leptin, hs C-reactive protein or CA15-3. In addition, no significant difference was noticed between positive and negative progesterone for serum leptin, hs C-reactive protein or CA15-3. A strong significant positive correlation was noticed between serum leptin and BMI in the control group; however, no significant correlation was noticed between serum leptin and BMI in the breast cancer patients. In conclusion, serum leptin may be used as a prognostic factor for breast cancer. Serum C-reactive protein in HER2/neu positive breast cancer patients is higher than in HER2/neu negative patients.

This study was conducted to evaluate the association of serum leptin and hs C-reactive protein with breast cancer. Two groups were included in the study. The first group included 45 newly diagnosed women with breast cancer. The second group included 42 women with benign breast lump as a control group. Blood samples (5 mL) were taken from the patient and the control groups and analyzed for serum leptin and hs C-reactive protein. Serum CA15-3 was also measured in breast cancer patients. The epidermal growth factor receptor 2 (HER2/neu), estrogen and progesterone receptors were determined in breast cancer patients by using immune-chemical method. Serum leptin was significantly higher (p ≤ 0.05) in breast cancer patients than that in the control group; however, no significant difference was noticed between the two groups for serum hs C-reactive protein. No significant difference was noticed between HER2/neu positive or negative in breast cancer patients for serum leptin or hs C-reactive protein. However, serum CA15-3 in HER2/neu positive patients was significantly higher (p ≤ 0.05) than that in HER2/neu negative patients. No significant difference was noticed between positive and negative estrogen breast cancer patients for serum leptin, hs C-reactive protein or CA15-3. In addition, no significant difference was noticed between positive and negative progesterone for serum leptin, hs C-reactive protein or CA15-3. A strong significant positive correlation was noticed between serum leptin and BMI in the control group; however, no significant correlation was noticed between serum leptin and BMI in the breast cancer patients. In conclusion, serum leptin may be used as a prognostic factor for breast cancer. Serum C-reactive protein in HER2/neu positive breast cancer patients is higher than in HER2/neu negative patients.

Low local recurrence rate without postmastectomy radiation in node-negative breast cancer patients with tumors 5 cm and larger

Soil pH is one of the main factors influencing the solubility and availability of trace elements in arable soils. Thus pH can affect the trace element contents of agricultural crops and thereby indirectly influence human health. The aim of this study was to determine Cd, Ni, Zn, Cu, Mn, Cr, Al and Se contents in spring wheat, potatoes and carrots (Cd, Ni and Zn) and estimate their correlations with certain soil factors (surface and subsurface soil pH and organic matter content) governing the plant availability of these elements. Commercial fields were sampled in Sweden in order to cover a wide range of soil types with respect to pH, soil texture and organic matter content. Concentrations of Zn, Mn, Ni (grain) and Cd (straw) in spring wheat (n=43); Cd, Ni, Zn, Mn, Cu and Al in potatoes (n=69); and Cd, Ni and Zn in carrots (n=36) showed significant negative correlations with surface soil pH (0–25 cm). The Se content of potatoes and Cr content of spring wheat straw were positively correlated with soil pH. Stepwise multiple regressions including a combination of soil pHs (0–25 and 25–50 cm) and organic matter contents (0–25 cm) showed that the organic matter content as well as the surface and subsurface soil pH significantly influenced concentrations of several trace elements in one or more of the studied crops. It was concluded that, if acid deposition together with other acidifying processes (fertilisation, harvest of biomass, etc.) are not balanced by a sufficient amount of liming there might be a decrease in the pH of arable soils, which, in turn will lead to decreased levels of Se in edible crops but an overall increase concentrations of other trace elements.

Investigation on Circulating Neonatal Nav1.5 (nNav1.5) and Its Antibodies in the Blood and Serum of 4T1 Orthotopic Mice Model and Breast Cancer Patients from Hospital Universiti Sains Malaysia (HUSM)

607 Background: Women with a history of radiation therapy (RT) for Hodgkin lymphoma (HL) have an elevated risk of breast cancer (BC). The characteristics, treatment and outcomes of these cancers, compared with sporadic breast cancers, are not well described. In particular, rates of synchronous and metachronous bilateral BC may be greater in patients with a history of RT for HL. Methods: Women diagnosed with BC between 1980 and 2006 following RT for HL were identified retrospectively from 8 institutions in North America. Each patient in this cohort was matched with 3 BC patients with no history of HL, from the same institution, by age at BC diagnosis, year of BC diagnosis and race. Information on patient and tumor characteristics, disease recurrence and new contralateral tumors was obtained from medical records. Analysis of metachronous contralateral breast cancer (CBC) was limited to women with a breast at risk, and adjusted hazard ratios (HRs) for CBC were estimated using Cox proportional hazards regression. Results: 253 BC patients with a history of RT for HL were matched with 741 patients with sporadic BC. The median interval between HL and first BC diagnosis was 19 years. Median age at BC diagnosis was 44 years and median follow-up was 5 years from BC diagnosis. Synchronous bilateral BC was diagnosed in 6% of the HL survivors and 2% of the matched patients with sporadic BC. Among women with unilateral disease, 21% of HL survivors and 3% of sporadic BC patients had a bilateral mastectomy. Overall 64 women with a breast at risk developed metachronous CBC. BC patients with a history of RT for HL had a greater rate of metachronous CBC (adjusted hazard ratio 3.3, 95% CI 2.1–5.8), controlling for patient, tumor and treatment characteristics. In addition, those who received chemotherapy for BC were less likely to develop CBC (adjusted HR 0.51, 95% CI 0.31–0.85), and those with a family history of BC in a 1st degree relative were more likely to develop CBC (adjusted HR 1.8, 95% CI 1.1–3.0). Conclusions: Women who develop BC after RT for HL have more than 3 times the risk of CBC than similar women with sporadic BC. Our findings support close follow-up for CBC in BC patients with a history of RT for HL and may inform decisions about prophylactic mastectomy in this population. No significant financial relationships to disclose.

FAM64A is a mitotic regulator which promotes cell metaphase-anaphase transition and is highly expressed in a cell-cycle-dependent manner. In this study, we examined the clinicopathological and prognostic significance of FAM64A mRNA expression in gynecological cancers. We conducted a bioinformatics analysis of FAM64A mRNA expression using Gene Expression Omnibus (GEO), The Cancer Genome Atlas (TCGA), xiantao, The University of ALabama at Birmingham CANcer data analysis Portal (UALCAN), and Kaplan-Meier (KM) plotter databases. FAM64A expression was elevated in breast, cervical, endometrial, and ovarian cancers when compared with normal tissue. Expression was positively correlated with white race, low T stages, infiltrating ductal carcinoma, or favourable PAM50 classification in breast cancer patients, and with clinical stage, histological grade and TP53 mutation, and endometrial cancer serous subtype. FAM64A expression was negatively associated with overall and/or recurrence-free survival rates in breast and endometrial cancer patients, while the opposite was observed in cervical and ovarian cancer patients. FAM64A functioned as an independent predictor of overall and disease-specific survival in breast cancer patients. FAM64A-correlated genes were involved in ligand-receptor interactions, and chromosomal, cell cycle, and DNA replication processes in breast, cervical, endometrial and ovarian cancers. Top hub genes primarily included cell cycle-related proteins in breast cancer, mucins and acetylgalactosaminyl transferases in cervical cancer, kinesin family members in endometrial cancer, and synovial sarcoma X and the cancer/testis antigen in ovarian cancer. FAM64A mRNA expression was positively related to Th2 cell infiltration, but negatively associated with neutrophil and Th17 cell infiltration in breast, cervical, endometrial, and ovarian cancers. FAM64A expression may be considered a potential biomarker reflecting carcinogenesis, histogenesis, aggressive behaviour, and prognosis in gynecological cancers. Impact statement What is already known on this subject? FAM64A is located in cell nucleolar and nucleoplasmic regions, and during mitosis it putatively controls metaphase-to-anaphase transition. FAM64A appears to regulate different physiological processes, including apoptosis, tumorigenesis, neural differentiation, stress responses, and the cell cycle. What the results of this study add? FAM64A expression was up-regulated in breast, cervical, endometrial, and ovarian cancers, and positively correlated with white race, low T stages, infiltrating ductal carcinoma, or favourable PAM50 classification in breast cancer patients, and with clinical stage, histological grade, and TP53 mutation, and a serous subtype in endometrial cancer. FAM64A expression was negatively associated with overall and/or recurrence-free survival rates in breast and endometrial cancer patients, while the opposite was observed in cervical and ovarian cancer patients. FAM64A functioned as an independent predictor of overall and disease-specific survival in breast cancer. FAM64A-correlated genes were involved in ligand-receptor interactions, chromosomal, cell cycle, and DNA replication processes, while FAM64A mRNA expression was positively related to Th2 cell infiltration but negatively correlated with neutrophil and Th17 cell infiltration in four gynecological cancers. What the implications of these findings for clinical practice and/or further research? In the future, abnormal FAM64A mRNA expression may serve as a biomarker of carcinogenesis, histogenesis, aggressiveness, and prognosis in gynecological malignancies.

This study aimed to use non-invasive methods to assess and compare the levels of oxidative indices and non-enzymatic antioxidants in breast and colorectal cancer (CRC) patients. Various studies have reported on lipid peroxidation, hydrogen peroxide (H(2)O(2)) and ferric-reducing antioxidant power (FRAP) levels in the serum of cancer patients but this is the first report that highlights the significance of urinary-advanced oxidative protein product (AOPP) in cancer patients. The levels of advanced oxidative protein product (AOPP), hydrogen peroxide (H(2)O(2)), malondialdehyde (MDA) which is a marker for lipid peroxidation and ferric-reducing antioxidant power (FRAP) were measured in urine samples of breast (n = 101) and colorectal cancer (n = 49) patients attending the Oncology Clinic, University Malaya Medical Centre, Kuala Lumpur and were compared with 95 age-matched healthy individuals. AOPP, H(2)O(2) and MDA levels in the urine were significantly higher in the CRC patients compared to the control subjects and breast cancer patients. In breast cancer patients, only AOPP level was elevated. FRAP level did not differ between breast and colorectal cancer patients but the levels were significantly lower compared to control subjects. Urinary oxidative indices such as AOPP, H(2)O(2), and MDA as well as FRAP could serve as useful non-invasive oxidative stress markers in colorectal cancer but only AOPP serves as a useful urinary oxidative biomarker in breast cancer.

Background: The role axillary lymph node dissection (ALND) in staging, prognostic assessment and local control in patients of breast cancer with clinically evident disease in the axilla is well established. ALND has its share of morbidity in the form of restriction of range of motion of arm, pain, paraesthesia and lymphedema influencing quality of life. This prospective study evaluates the effect of infiltrative anaesthesia injected along the nerve bundles exposed during ALND in reducing the restriction of range of motion of arm and pain scores following ALND. Methods: A total of 60 ALND in patients of operable invasive breast cancer were randomized in to two groups:- Group 1: The control group where patients of ALND were managed in the postoperative period with standard analgesia of injection acetaminophen 1 gm 8 hourly and early arm and shoulder movements from the evening of the procedure. Group 2: The test group had 5 ml of 0.125% Bupivaccaine injected each around the three nerve bundles exposed during ALND along with early arm and shoulder exercises and standard analgesia similar to the control group. Medical records of patients were analysed for age, laterality, clinical staging, histopathological parameters and surgical complications. Range of motion of ipsilateral at the shoulder and pain scores as recorded by visual analog scale (VAS) were objectively noted on day 0 to day 28 postoperatively at various intervals. Results: The range of motion at ipsilateral shoulder was measured by flexion, abduction and extension. The degrees of flexion and abduction were found to have statistically significant difference on Days 0, 3 and 7 in the test group in comparison to control group (p=0.001 for flexion on all Day 0,3 and 7 and p=0.009 for Day 0, p=0.001 for Day 3 and p<0.001 for Day 7 for abduction). Extension was better in the test group on Days 0 and 3 (p<0.001 and p=0.005 respectively). Pain scores assessed by VAS were significantly lower in test group when compared to control group on Days 0,1, 3 and 7 (p ≤ 0.01). There was no statistically significant difference in the restriction of flexion, extension and abduction and pain scores on Day 28 between the groups. Analgesia requirement was significantly reduced in the test group on days 0 and 1 (p ≤ 0.05). Conclusion: Infiltrative anaesthesia with 0.125% bupivaccaine along the nerve bundles exposed during ALND improves range of motion at shoulder and pain scores in the immediate postoperative period in patients of breast cancer.

Background. Some studies have shown that exercise increases breast cancer (BC) patients’ quality of life (QoL) during and after treatments. The aim of this study was to investigate the effect of an specific exercise intervention in QoL and in the exercise levels of these patients. Methods. A randomized controlled trial evaluated an intervention (IG) vs a control group (CG) in early stage BC patients who recently finished chemo &amp; radio therapies. Intervention consists on controlled group classes combining aerobic and resistance activities. CG maintained their previous lifestyle. QoL, leisure-time exercise levels (LTEL), chest (CMS) and legs maximal strength (LMS), physical capacity (PC) and some psychological variables, were assessed at baseline and after 3 months in both groups. Women who participated in the IG were followed up (FU) after 6 months. Statistical analysis (SA) were performed using unpaired t-test on continuous variables comparing CG vs IG, considering possible confounders. Pearson correlation analyses were employed to examine possible correlations. 6 months FU data were analyzed comparing baseline and after intervention results using non-parametric test (n=13). SA was performed with SPSS v18 software. 95% CI was calculated and statistical significance level of p &amp;lt; 0.05 was used. Results. 59 women (median 48.97±8.35 years old) completed this study. No differences between groups were observed at baseline. Average attendance rate to the program was 89%. There was a significant rise in LTEL (t123=16.33; p=0.0001) and in QoL (t123=2.88; p=0.005) comparing IG vs CG. Results also showed a correlation between QoL and LTEL in patients of IG only (r=0.22; p=0.013). Significant differences between groups were observed in both physical and psychological variablesafter training program. Table 1. Results summary comparing intervention and control group.VARIABLESIntervention Group (n= 27) Mean±SDControl Group (n=32) Mean±SDFollow-Up (n=13) Mean±SDQoL*+112.88±17.74±103.11±19.26110.96±14.40LTEL*+45.11±14.6114.87±4.7834.56±19.51Body Fat Mass34.63±6.736.47±7.4735.74±4.56Muscle mass41.3±3.1638.78±8.5141.48±2.01Strength Index*+2.6±0.832.12±0.692.32±0.58CMS*+45.89±11.0731.47±9.8753.76±12.42LMS*+93.07±27.369.00±24.94114.65±24.29PC*+32.58±4.9627.08±3.7332.11±7.10FACT-F *+135.94±18.20124.00±24.20138.24±17.49SF-36 Physical Dimension*+48.49±4.8345.61±5.8249.75±2.75SF-36 Psychological Dimension*+43.00±8.1137.03±12.5844.33±7.33Depression*+6.83±7.8312.55±10.775.88±5.19*Significant differences were found between control and intervention group. + Significant differences were maintained between baseline and follow-up assessments. No differences taking into account confounders were observed.Variable changes were maintained in FU participants assessed after 6 months. Correlation between QoL and LTEL (r=0.52; p=0.008) and significant weight loss (χ22=6.08; p=0.048) were observed. Conclusion. These results suggest that a specifically designed BC exercise program increases LTEL, which correlates to a better QoL. This may reduce psychological and physical side effects of systemic treatment in patients with early BC that have recently finished treatments, even producing lifestyle changes in BC patients that could be long lasting. Citation Format: Soraya Casla, Sara López-Tarruella, Yolanda Jerez, Iván Márquez-Rodas, Ricardo Cubedo, Isabel Calvo, Ana Martinez, Sara Cano, Rubén Barakat, Miguel Martín. Exercise intervention to run away from breast cancer treatment side effects: An integrative approach [abstract]. In: Proceedings of the Thirty-Seventh Annual CTRC-AACR San Antonio Breast Cancer Symposium: 2014 Dec 9-13; San Antonio, TX. Philadelphia (PA): AACR; Cancer Res 2015;75(9 Suppl):Abstract nr P5-15-08.

The trigger for some cases of juvenile diabetes has been suggested to be an interaction between a virus and various trace elements. Infection with human coxsackievirus B3 (CB3) in the murine model results in viral replication and inflammation in the pancreas. To determine how infection affects the trace element balance in the pancreas. Concentrations of the following trace elements were measured in the serum and pancreas during the early phase (days 1 and 3) of CB3 infection in female Balb/c mice: aluminium, arsenic, cadmium (Cd), calcium (Ca), cobalt (Co), copper (Cu), iron (Fe), lead (Pb), magnesium (Mg), manganese (Mn), mercury (Hg), selenium, silver, vanadium (V), and zinc (Zn). The trace element concentrations were measured through inductively coupled plasma mass spectrometry. The histopathology was established by hematoxylin-eosin techniques and immunohistochemical staining of both CD4 and CD8 cells of the pancreas. Infected mice developed expected clinical signs of disease. The only changes at day 1 occurred in the serum, with a pronounced decrease in the Zn concentration and a small increase in the V concentration. At day 3, concentrations of several trace elements, including Cu, Zn, Fe, Ca, V, and Mn, showed pronounced changes in both the serum and the pancreas. Ca, Cu, Mg, Mn, and V, but none of the potentially toxic elements, accumulated in the pancreas. Cu and V concentrations increased in the serum as well. Several trace element changes, preceding the development of pancreatitis, occurred in the pancreas in this viral infection, the exact pathogenic interpretation of which warrants further studies.

Interannual patterns of variation in concentrations of trace elements in arms of Octopus vulgaris

Актуальность мониторинга содержания тяжёлых металлов в водах Атлантического сектора Антарктики определяется необходимостью современной оценки качества водной среды для принятия ответственных решений по сохранению морских живых ресурсов в этом уникальном регионе Мирового океана. Цель проводимых исследований — получить новые данные об уровнях и пространственном распределении концентрации микроэлементов, преимущественно тяжёлых металлов, в поверхностной воде. Отбор проб поверхностной морской воды производили в антарктической экспедиции 79-го рейса НИС «Академик Мстислав Келдыш» на 21 станции в районе проливов Дрейка, Брансфилда, Антарктика, а также в морях Уэдделла и Скотия. Экстракцию и концентрирование растворённой формы 13 микроэлементов (Be, Se, Sb, Tl, V, Pb, Cd, Cu, Zn, Ni, Mo, Co и Fe) осуществляли с помощью диэтилдитиокарбамата натрия и четырёххлористого углерода (CCl4). Измерение элементов проводили масс-спектрометрическим методом. Только для Mo на девяти станциях, расположенных в проливах Дрейка и Брансфилда, в северном районе моря Уэдделла, а также возле южного побережья острова Огненная Земля, отмечали превышение его концентрации в морской воде в 1,2–2,8 раза по отношению к ПДК микроэлементов в воде рыбохозяйственных объектов РФ (ПДКРХ). Согласно международным нормативно-правовым актам, таким как «Голландские листы», зарегистрированы единичные случаи превышения MPC (maximum permissible concentration — ПДК при краткосрочном воздействии) для Cd и Zn, а также превышение TV (target value — контрольные уровни при хроническом воздействии) для Cu, Pb, Cd, Zn, Se и Co на нескольких станциях. Исследования показали, что, несмотря на ограниченный режим антропогенной нагрузки в этом регионе Южного океана, в морской воде отдельных районов Атлантического сектора Антарктики в современный период зафиксированы повышенные концентрации некоторых микроэлементов, включая тяжёлые металлы. Необходимо дальнейшее изучение источников поступления и особенностей распределения микроэлементов в морских водах Атлантической части Антарктики для объяснения происходящих процессов, а также для принятия мер по рациональному управлению и экологически приемлемому природопользованию в Антарктическом регионе.

Background. Physical exercise increases breast cancer (BC) patients' survival. However, only about two thirds of them follow the American Guidelines of Exercise to cancer survivors. The aim of this study was to examine the effect of an exercise intervention in breast cancer patients' lifestyle. Methods. A randomized clinical trial evaluated an intervention (EXE) vs. a control (CON) group in early stage BC patients who recently finished the chemo and radio (neo) adjuvant. Intervention consists on exercise group classes combining aerobic and resistance activities designed specially for the necessities of these patients. Intensity was increasing gradually and intervention was controlled by a qualified in oncologic exercise specialist. CON group maintained their lifestyle without changes. Leisure-time exercise levels (LTEL), quality of life (QoL), grip strength index (GSI), physical capacity (VO2max) and fatigue, were assessed at baseline and after 3 months in both groups. Women who had been participated in the intervention group were followed up after six months to know if lifestyle levels were maintained. ANCOVA and Pearson Test were used to analyze the continuous variables of baseline and final data and ANOVA test was used to analyze the follow-up data. A 95% of CI was calculated and p &amp;lt; 0.05 was determined as statistically significant. Results 89 women, aged 49.06±8.75, completed the study, 44 in EXE group and 45 in CON group. Adherence rate was on average of 89%. Main results are presented in the Table. Main ResultsMEASUREEXE GroupEXE GroupEXE GroupCON GroupCON Group BLFFUBLFQoL*+107.53±17.99112.88±17.74110.96±14.40104.94±17.65103.11±19.26LTEL*+18.73±20.5345.11±14.6134.56±19.5116.02±8.5215.04±4.75GSI*+2.07±1.082.60±0.832.48±0.582.03±0.722.12±0.69VO2max*+26.99±4.3532.58±4.9632.11±7.1027.73±4.8227.08±3.73Fatigue *+130.09±19.68135.94±18.20138.24±17.49124.83±24.66124.00±24.20BL=baseline; F= Final; FU= Follow-Up. *Significant differences between EXE and CON. + Significant differences between BL and FU. There was a significant improvement in LTEL (p=0.0001) and in QoL (p=0.0001) comparing EXE vs. CON group. In addition, results showed a correlation between this two variables in EXE group (r=0.22; p=0.013). These significant improvements, as well as the mentioned correlation, were maintained in the 6-months follow-up assessment in EXE group. Significant differences between groups were observed in GSI (p=0.004), and in VO2max levels (p=0.001). EXE group showed a significant improvement in fatigue levels compared with CON group (p=0.0001). All these significant improvements were maintained in the 6-months follow-up assessment in EXE group, as well as previous variables. Conclusion. These results suggest that an exercise intervention increases LTEL correlated to a better QoL, improving patients' lifestyle that could be long lasting. These changes may ameliorate psychological and physical BC treatments side effects, such as fatigue, in patients with early breast cancer that has recently finished adjuvant treatments. Citation Format: Casla S, López-Tarruella S, Jerez Y, Marquez-Rodas I, Barakat R, Martín M. An integrative intervention to change breast cancer patients' lifestyle: A medical challenge. A randomize controlled trial. [abstract]. In: Proceedings of the Thirty-Eighth Annual CTRC-AACR San Antonio Breast Cancer Symposium: 2015 Dec 8-12; San Antonio, TX. Philadelphia (PA): AACR; Cancer Res 2016;76(4 Suppl):Abstract nr P1-10-10.