Selenium nanoparticles (SeNPs) synthesized through green routes have emerged as promising nanobiostimulants in sustainable agriculture due to their ability to enhance plant growth and antioxidant defense. The aim of this study was to evaluate the biostimulant effect of SeNPs on Capsicum annuum at two stages of crop development to characterize the response to SeNP exposure and identify concentration-dependent effects and application methods. Physiological indicators, including growth, photosynthetic pigment content, and antioxidant activity, were evaluated. Different concentrations of SeNPs were tested during germination, and dosage and two types of application were compared during the vegetative phase in a hydroponic experiment. SeNPs at concentrations of 1.25, 2.5, 5, 10, 20, 40, and 80 µM were applied to chili seeds for 20 days. The plants were exposed to SeNPs concentrations ranging from 1.25 to 80 µM, applied through the roots and leaves. Germination parameters were not significantly affected except for the seed vigor index, which increased at all concentrations, particularly at 20 µM. Low to moderate doses (1.25–20 µM) acted as biostimulants, enhancing plant height, root length, biomass accumulation, photosynthetic pigment content, and phenolic and flavonoid compound synthesis. Conversely, high doses (80 µM) induced phytotoxic effects, especially via root exposure, reflected by growth inhibition, and reduced chlorophyll content. Foliar application demonstrated a systemic biostimulant response, improving root growth and photosynthetic activity without toxicity symptoms. Antioxidant assays (DPPH and ABTS) revealed dose-dependent modulation of redox balance, suggesting adaptive responses to SeNP-induced oxidative conditions. These findings highlight the potential of SeNPs as biostimulants that improve physiological performance in chili plants, while emphasizing the importance of an optimal dosing and application method for sustainable nanotechnology-based crop management.

Microbial inoculation represents an environmentally friendly biocontrol strategy that can enhance soil quality, improve crop growth efficiency, and promote sustainable agriculture. However, the long-term effects and ecological safety of non-native microbial inoculants in soil remain uncertain. Here, we explore and evaluate a safer and potentially more effective inoculation strategy—the reintroduction of native microbiota—to maintain agricultural ecosystem health. Native microbiota were extracted from black soil in northeastern China and reintroduced into the indigenous soil. Two treatments were established: original soil (control) and original soil with a mixture of native microbiota, each with nine replicates. Soil samples were collected at 0, 21, and 90 days post-inoculation. Using high-throughput sequencing and agronomic chemical analyses, we dynamically monitored soil nitrogen, phosphorus, and potassium contents, as well as microbial community composition. Crops were harvested at day 90 to measure dry weight, fresh weight, and SPAD values. The results revealed that the number of colonizing species was lower than the number of inoculated species, yet crop agronomic traits and chemical composition were significantly improved, particularly SPAD values and total phosphorus content. Soil abiotic factors exhibited limited resistance but retained partial recovery capacity, showing a notable increase in readily available potassium at days 0 and 21. Native microbiota inoculation promoted positive synergistic interactions within the microbial community. Furthermore, this study underscores the practical significance of cultivable microorganisms in agricultural applications. Collectively, our findings demonstrate the feasibility of native microbiota reintroduction, highlighting its potential to optimize soil microbial communities, enhance soil properties, and improve crop performance, thereby providing a scientific basis for soil remediation and sustainable agriculture.

The analysis of water and emission efficiency in cropping systems is vital for sustainable agriculture in Mediterranean regions, which face increasing water shortages. This study offers a site-specific assessment of the Water Footprint (WFP) and Carbon Footprint (CFP) of organic cotton grown under Mediterranean conditions, integrating environmental indicator measurements with economic valuation of greenhouse gas (GHG) emissions via the EU Emissions Trading System (ETS) and the Social Cost of Carbon (SCC). Experiments were carried out at three sites with different soil types, testing two cultivars (Armonia and ST-318) under three irrigation scenarios: severe water deficit (I30), moderate water deficit (I70), and full irrigation (I100). The results reveal significant site-specific variability, with average WFP_lint values ranging from about 1.440 m3 per ton at the most productive site to over 4.100 m3 per ton at the least productive site. Similarly, CFP_lint is lower under high-yield conditions, emphasizing the strong influence of yield on mass-based indicators. At the Carboj and Primosole sites, shifting from (I30) to I100 results in roughly a 50% reduction in emissions, while at Buonfornello, increased irrigation does not consistently produce benefits. The cultivar response is key: Armonia shows greater resilience to water stress, while ST-318 performs best with full irrigation. Overall, the findings highlight that the sustainability of the Mediterranean cotton system depends on factors such as yield performance, site-specific conditions, and cultivar choice.

Soil-borne diseases have become increasingly serious due to continuous planting. Soil fumigation may be inadequate because of the persistence of soil-borne pathogens on ginger seed rhizome. A combined strategy of soil fumigation and seed rhizome disinfection would be necessary to achieve synergistic control. In this study, the approach of soil fumigation with chloropicrin (CP) coupled with seed rhizome disinfection (Copper, Cu) was first adopted to evaluate the synergistic effects on soil physicochemical properties, enzyme activities and microbial communities, and therefore reveal mechanisms for soil microecological health and crop yield promotion. The results showed the comprehensive strategy could reduce NO3−-N content, and the activities of soil enzymes, while increased NH4+-N content, EX-Cu, and OXI-Cu content, which were positively correlated with ginger yield but negatively correlated with soil-borne pathogens and plant mortality. On the other hand, there was a reduction in bacterial diversity and richness, which was positively correlated with the abundance of soil-borne pathogens. Moreover, some beneficial soil microorganisms’ relative abundance (such as Firmicutes, Actinobacteria, Bacillus, and Sphingomonas.) was increased. The strategy decreased the abundance of Fusarium spp. and Phytophthora spp. by 49.41–90.07% and 43.34–89.21%, respectively. Compared with other treatments, the combination decreased the ginger mortality by 5.70–57.02% and increased the growth of ginger plants and yield by 3.58–139.96%, and 13.11–399.74%, respectively. This study highlights a prospect to promote ginger growth and yield by blocking the transmission of primary infection pathogens in ginger cultivation and improving soil ecological environment.

The longitudinal study investigated the impact of age at first mating (AFM) on milk yield (MY) across the productive lifespan of Alpine goats born between 2005 and 2018. Data from 740 animals across three herds and 3200 lactations were analyzed. The AFM of the studied population ranged from 7 to 23 months. The impact of AFM on MY was estimated using a linear mixed model, accounting for the fixed effects of parity, litter size, season, herd, and suckling and milking durations, with the individual goat included as a random effect to control for repeated measures. The impact of AFM on lifetime production was estimated by regressing total milk yield (TMY) and number of lactations (TNL) on AFM, while accounting for herd effect. The study revealed a notable shift in productivity patterns across the animal’s life. Every additional month of AFM significantly increased milk yield in the first lactation (13.28 kg; p < 0.001), but this influence vanished in subsequent parities (p > 0.05). These higher initial yields were insufficient to compensate for the losses caused by a shortened productive lifespan. Specifically, each month of mating delay resulted in a loss of ~0.08 TNL and 34 kg TMY, totaling ~1 lactation and ~400 kg of milk for a 12-month delay. Results suggest that earlier mating may improve lifetime productivity under intensive production systems.

In areas with frequent wildlife activity, coordinating biodiversity conservation with agricultural production is a critical issue for achieving agricultural sustainability. This study uses farm household survey data collected in 2022 from Asian elephant distribution areas in Yunnan Province, China. It systematically evaluates the effects of Human–Elephant Conflict (HEC) and the wildlife damage compensation policy on farm households’ farmland use behavior. Focusing on farmland adjustment behavior under the context of biodiversity conservation, we develop an analytical framework of “HEC–policy intervention–farm household farmland use behavior.” Using survey data from 1276 farm households, we examine the effects of HEC on farmland transfer-out and farmland abandonment. We also analyze the moderating role of the wildlife damage compensation policy. In addition, we explore the heterogeneity between areas inside and outside nature reserves. The results show that: (1) HEC significantly increase the likelihood of farmland transfer-out and farmland abandonment among farm households; (2) the wildlife damage compensation policy partially mitigates the positive effects of HEC on farmland transfer-out and farmland abandonment; and (3) the effects of HEC on farmland transfer-out and farmland abandonment are more pronounced for farm households outside nature reserves. The wildlife damage compensation policy shows a stronger inhibitory effect on farmland transfer-out inside nature reserves. In contrast, it has a stronger inhibitory effect on farmland abandonment outside nature reserves. From the perspective of farmland use, this study reveals how HEC and policy intervention influence farm households’ farmland allocation behavior. It also provides empirical evidence for improving wildlife damage compensation mechanisms. In addition, the findings help promote synergy between agricultural sustainability and biodiversity conservation.

This study evaluated the effects of fly ash (F) and effective microorganisms (EM) on nutrient dynamics and heavy metal transformations during vermicomposting of camel manure (CM). Four treatments (CM, CM + F, CM + EM, and CM + F + EM) were arranged in a completely randomized design and monitored over 12 weeks. Significant (p < 0.05) treatment and time interactions were observed for pH, NH4-N, Mn, Pb, and Mo. The addition of EM resulted in a greater decline in pH compared to other treatments. After 12 weeks, Olsen P increased from 300.62 to 398.71 mg/kg in CM + EM, while NH4-N increased markedly from 22.74 to 86.62 mg/kg. In contrast, NO3/NO2-N declined in EM-amended treatments but increased in the control and CM + F. Trace metal concentrations generally increased due to mass reduction during vermicomposting yet remained within internationally acceptable limits. Germination index (GI) values varied significantly among crops and treatments, ranging from phytotoxic to non-phytotoxic responses. Although CM + EM produced superior nutrient enrichment, several vegetables exhibited GI values below 50%, indicating potential phytotoxicity for sensitive crops. In case of established crops for which nutrient supply outweighs early phytotoxic concerns, CM + EM represents the most agronomically beneficial option. Future studies should explore blending CM + EM and CM + F with stabilizing amendments such as biochar to optimize nutrient availability while minimizing salinity and phytotoxic risks.

Against the backdrop of escalating global climate challenges, minimizing carbon emissions while enhancing energy efficiency in rice production has emerged as a core pathway toward achieving agricultural carbon neutrality. A two-year field study conducted in the Yangtze River Delta evaluated three rice cultivation practices: the farmers’ practice pattern (FPP), surface-applied controlled-release fertilizer with machine transplanting (S-CRF), and side-deep applied controlled-release fertilizer with machine transplanting (SD-CRF). Compared to FPP and S-CRF, SD-CRF increased grain yields by 11.3% and 9.2%, respectively, while reducing total energy input by 2.5% and 2.4%. It lowered the carbon intensity of production by 9.7% and 8.2% relative to FPP and S-CRF, primarily through reducing fertilizer/labor-associated carbon inputs and enhancing carbon-use efficiency via higher yield. Economically, SD-CRF outperformed traditional practices, achieving an 81.8% increase in net income and a 37.4% higher benefit-to-cost ratio compared with FPP, respectively, driven by labor cost savings and improved productivity. Notably, SD-CRF reduces labor input by 40.0% compared with FPP, simplifies fertilization operations, lowers farmers’ operational technical thresholds, and effectively boosts their economic income. Data envelopment analysis (DEA) further validated SD-CRF’s superior eco-efficiency, highlighting its dual advantage in balancing yield enhancement and environmental sustainability. Further clarification of SD-CRF application technical indicators, refinement of agronomic practices and machinery efficiency, and promotion of the integrated system’s synergistic benefits and scalable adoption are required to support global sustainable food systems and carbon neutrality goals.

This study evaluated the effects of biostimulant treatments on the ionic composition and yield of ‘Formosa’ papaya (Carica papaya L.) subjected to varying water replacement levels. The research utilized a randomized complete block design in a split-plot scheme comprising three irrigation depths (100%, 75%, and 50% ETc) and four biological treatments: control, Trichoderma harzianum, Ascophyllum nodosum, and Bacillus aryabhattai. Contrary to initial expectations, water restriction was associated with increased yield and leaf concentrations of zinc (Zn) and nitrogen (N), challenging the hypothesis that water restriction limits production. Quantitative results showed that T. harzianum under 75% ETc attained 93.29 kg plant−1, whereas the control at 50% ETc recorded 19.14 g kg−1 of N. Under 50% ETc, B. aryabhattai increased the bacterial population to 10.46 log10 CFU g−1 soil compared to the control. The T. harzianum-based biostimulant reduced leaf sodium (Na) under 75% ETc and maintained the nutrient accumulation order K > N > Ca > Fe > Mn > Zn > Cu > Na. Conversely, B. aryabhattai and A. nodosum improved yield under 100% ETc through N accumulation. This study confirms that microbial and seaweed-based biostimulants mitigate water stress through rhizosphere modification and nutritional homeostasis, offering a practical strategy for sustainable fruit production in semi-arid regions.

Tourists’ environmentally responsible behavior (ERB) is a key requirement in the promotion of the sustainable development of tourism destinations. Although extensive studies have explored the antecedents of tourists’ ERB, research within the context of rural tourism remains limited. Addressing this gap and guided by the Stimulus–Organism–Response theory, this study develops an integrated model in which nature relatedness and place attachment serially mediate the effect of the rural tourscape on tourists’ ERB. Based on 398 valid questionnaires collected from Huangling Village in Wuyuan, China—a UNWTO Best Tourism Village—data were analyzed using structural equation modeling. The findings reveal that (1) the ambience and agricultural products dimensions of the rural tourscape have a direct positive impact on tourists’ ERB, and (2) both nature relatedness and place attachment serve as independent mediators between the rural tourscape and tourists’ ERB. Furthermore, there exists a serial mediating pathway of “nature relatedness → place attachment” between them. This study uncovers the complex mechanisms underlying the formation of tourists’ ERB in rural tourism settings. By introducing nature relatedness as a mediator, it offers new insights for both theoretical advancement and the practical management of tourists’ ERB.