- Research Article
- 10.21423/aabppro20259420
Ultrasound-guided cerebrospinal fluid collection at the atlantoaxial space in mature cattle with a comparative evaluation of lumbosacral cerebrospinal fluid collection
- Apr 28, 2026
- American Association of Bovine Practitioners Conference Proceedings
- J Brozek + 4 more +4
The introduction of an alternative cerebrospinal fluid (CSF) collection site in equine practice at the atlantoaxial (AA) space has grown in popularity with increased proficiency and application of ultrasound-guided techniques. In cattle, CSF is routinely collected at the lumbosacral (LS) space or the atlantooccipital space, which requires general anesthesia. Although described in camelids, the technique has not been evaluated in cattle. The objectives of the present study were to demonstrate that ultrasound-guided AA CSF collection could be performed safely and reliably in standing beef cattle and that ultrasound guidance between the first and second cervical vertebrae (C1-C2, i.e., AA) would minimize iatrogenic blood contamination in samples compared to the LS centesis site.
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