All Research Papers Published In Biochemical Engineering Journal

Research Article
10.1016/j.bej.2026.110173

Mechanisms and influencing factors in Ni2+ removal through microbially induced calcite precipitation (MICP) by indigenous ureolytic bacteria

Mar 18, 2026
Biochemical Engineering Journal
Kai Yang + 4 more +4

Research Article
1
10.1016/j.bej.2025.110043

Enhanced microalgae cultivation and simultaneous achievement of nutrient removal from actual anaerobic digestion effluent of a full-scale kitchen waste plant

Mar 01, 2026
Biochemical Engineering Journal
Wenwei Zhang + 5 more +5

Research Article
1
10.1016/j.bej.2025.110052

Comparative evaluation of a hybrid membrane treatment train versus full-scale aerobic/anoxic activated sludge system: C-N removal performances, syntrophic microbial dynamics, and energy savings

Mar 01, 2026
Biochemical Engineering Journal
Santhana Krishnan + 6 more +6

Research Article
10.1016/j.bej.2025.110051

Hydrocyclone-enhanced nutrient removal and sludge densification in activated sludge system: Insights into microbial response

Mar 01, 2026
Biochemical Engineering Journal
Wentao Xu + 7 more +7

Research Article
10.1016/j.bej.2025.110013

Investigating the effect of media composition on growth and mAb production in CHO cells using a piecewise hybrid dFBA-PLS framework

Mar 01, 2026
Biochemical Engineering Journal
Zahra Negahban + 5 more +5

In this study, we present a hybrid modeling framework that integrates piecewise Partial Least Squares (PLS) regression with Dynamic Flux Balance Analysis (dFBA) to simulate and optimize Chinese Hamster Ovary (CHO) cell fed-batch culture. Twenty-four Ambr15 experiments were conducted to systematically vary feed and inoculum compositions. Time-resolved metabolite, biomass, and Monoclonal antibodies (mAb) concentrations were collected and modeled. The hybrid model achieved high prediction accuracy (Normalized Mean Squared Error (NMSE) < 0.15 for most metabolites) and provided interpretable flux profiles. Multivariate analysis revealed consistent metabolic signatures tied to media formulation, where specific feed–inoculum combinations drove shifts in glycolysis, TCA cycle flux, and nitrogen metabolism. These insights demonstrate the model’s capacity to capture key metabolic adaptations and support data-driven media optimization in CHO cell culture. • A piecewise hybrid model developed and validated for CHO fed-batch culture. • Bioreactor experiments were performed with 24 different media blends. • A balanced production\consumption of key metabolites results in higher mAb titer. • Specific feed–inoculum blends drive shifts in glycolytic and TCA cycle fluxes. • Hybrid model results in good predictions for varying media composition (NMSE<0.15).

Research Article
10.1016/j.bej.2025.110044

Microbial synthesis of L-fucose from glycerol and glucose in engineered Escherichia coli

Mar 01, 2026
Biochemical Engineering Journal
Yongwei Zhang + 4 more +4

Research Article
2
10.1016/j.bej.2025.110008

Bamboo biochar boosts methane production, microbial resilience, and economic performance in ammonia-rich anaerobic digestion

Mar 01, 2026
Biochemical Engineering Journal
Kazutaka Ueno + 5 more +5

Research Article
10.1016/j.bej.2026.110166

Modeling of Low-Energy Electrochemical Disinfection and Evaluation of Performance via Response Surface Methodology

Mar 01, 2026
Biochemical Engineering Journal
Hala Hossam + 5 more +5

Research Article
10.1016/j.bej.2025.109999

Process-based strategies improve xylitol production by recombinant S. cerevisiae from sugarcane straw hydrolysate

Feb 01, 2026
Biochemical Engineering Journal
Thiago I Gaspar + 3 more +3

Research Article
10.1016/j.bej.2025.109991

Stepwise enhancement of selectivity in hydrophobic charge-induction chromatography through grafted and dual-ligand systems

Feb 01, 2026
Biochemical Engineering Journal
Wei Shi + 7 more +7