Aim.The aim of the work is the study of a spectral-fluorescent response of a series of dioxaborine dyes in the presence of serum albumins.Methods.UV-Vis absorption and fluorescence spectroscopy.Results.The fluorescent response of eight polymethine dyes (six of them being dioxaborine-based) in the presence of human, bovine, and equine serum albumins was studied and compared to the response in the presence of DNA and ovalbumin.It was shown that dianionic dioxaborine A74 and thiobarbituric-based merocyanine In4tb demonstrated the most promising properties as fluorescent probes for human serum albumin detection, with fluorescence intensity increasing by 62-and 56-fold, respectively.Meanwhile, A74 shows the highest selectivity to HSA among other serum albumins: in the presence of ESA and BSA, the signal increased by only 28-fold and 7.9-fold, respectively.Thus, one can suppose that A74 may be sensitive to slight variations in the globular conformation of serum albumins.Besides, dioxaborine merocyanines B33 and B36 demonstrated nearly 10-fold fluorescence intensity increase in the presence of serum albumins.Conclusions.The most promising dyes A74 and In4tb can be further studied for various applications that require fluorescent detection or imaging of HSA, and, in the case of A74, as a probe sensitive to minor changes in the HSA globular structure.

Background.Monoclonal antibodies (mABs) and their fragments are widely used for therapeutic, diagnostic, research and other purposes.At the same time, the de ve lop ment of this group of pharmacotherapeutic agents still encounters a number of challenges.One of the areas of improvement in translational (T) studies is the use of pharmacometrics in silico, which can be explained by significant progress in the computing facilities, increased data volume and information availability.Aim.The aim of the study was to identify trends and directions of using physiologically based pharmacokinetic modelling (PBPK) in silico in translational studies of mABs.Methods.The analysis of scientific publications of the PubMed database for the period up to May 2024 inclusive was used.The keywords used in the advanced search were combinations of the words "PBPK" and "mAB", "antibody", "antibody drug conjugate", and combinations of "PBPK" with drugs (mABs) that fit our scope.Results.In total, 129 scientific articles were identified according to the selected criteria, including 110 (85%) original and 19 (15%) review articles.Although the first paper was published in 1994, 90% (116 publications) were published in the last 10 years (2013-May 2024), including 61% (79) in the last 5 years (2019-May 2024).Over the past 5 years, more than two thirds of original publications were related to the basic (T0) and preclinical (T1) stages of translational studies.43% of the papers were devoted to the study of parameters that may affect the absorption, distribution, metabolism and excre tion (ADME) of mABs, including physicochemical char