The use of light-transparent acetate sheet (LAS) may generate variations in the height measured in the sward. However, we compared the use or non-use of LAS to measure the average sward height of marandu palisadegrass (Urochloa brizantha syn. Brachiaria brizantha cv. Marandu) pastures. In the first experiment, the methods were applied in pastures with different average heights (15, 25, 35, and 45 cm), managed under continuous stocking with sheep, and with predominance of vegetative tillers. The shorter pastures (15 and 25 cm) had similar heights with or without the use of the LAS. However, in the taller pastures (35 and 45 cm), the use of LAS resulted in lower height values, compared with its absence. In the second experiment, we compared both methods for measuring the height of deferred marandu palisadegrass and fertilized with two nitrogen levels (50 and 200 kg ha-1) and with high presence of reproductive tillers. A completely randomized design with four replicates was adopted. With 200 kg ha-1 of N, the canopy height was greater than when it was fertilized with 50 kg ha-1 of N. With the use of LAS, the height of deferred and reproductive canopy was greater than without it. In a taller marandu palisadegrass pasture with predominance of vegetative tillers, the use of LAS results in smaller height, contrary to what occurs in deferred swards with reproductive tillers.

Cathranthus roseus also known as periwinkle, an ornamental plant contains several medicinal values, was found with the symptoms of little leaf and witches’ broom at Shahjahanpur location with the incidence of up to 8%. The phytoplasma etiology was confirmed through scanning electron microscopy examination in all the four-leaf samples. Molecular analysis through PCR with universal primer pairs P1/P6 followed by nested PCR with R16F2n/R16r2 primers yielded ~1.2kbp amplicons in all the four symptomatic leaf samples. One amplicon was eluted, purified, sequenced, and used in BLASTn searches, which showed maximum identity of periwinkle isolate with several isolates of 16SrIX group of phytoplasma. Further, phylogenic analysis and in silico RFLP confirmed the association of 16SrIX-C subgroup phytoplasma in little leaf and witches broom plants which is the first report from India.

Pastures cover, in Brazil, around 165 million ha, most of them are at some degradation level. Recovering these pastures is essential. The objective of this study was to evaluate the sensibility of soil organic matter (SOM) and its physical attributes in pasture soil at different degradation levels (1 to 4), Capoeiras (1 and 2) and secondary Forest (Control). Samples were collected at four depths (0.00-0.05, 0.05-0.10, 0.10-0.20 and 0.20-0.40 m). We verified higher values for SOM and hydraulic conductivity (Ko) in Forest, especially in the most superficial layer (0.00-0.05). We noted low values for macroporosity in this area. In all areas under Pastures lower values for Ko were observed in the superficial layer (0.00-0.05 m) when compared to layers 0.05-0.10 and 0.10-0.20 m, which indicates that the pasture management and cattle trampling negatively affect the physical quality of the pastures. For field capacity and wilting point the average values were observed in Pasture 4 (more degraded), which caused higher values for water availability. According to the principal components analysis, except for SOM at 0.20-0.40 m, all variables studied presented correlation above 0.6. From grouping analysis, we separated 3 groups: Forest; Capoeiras 1 and Capoeira 2 and Pastures 1 to 4.

Despite being associated with more accuracy, the indirect bonding procedure is not yet the gold standard, probably because of sensitivity of the numerous variables that must be controlled. The aim of this article was to present a modified, standardized, and low-cost indirect bonding technique that allows this procedure to be performed successfully. The technique covers an initial clinical stage, to obtain the models; a laboratory stage, which involves placement of brackets on the models following the facial axis of the clinical crown and the labial projection of the marginal ridges of the posterior teeth and construction of transfer tray using hot glue; and a second clinical stage, to properly transfer the brackets to patient’s teeth. Hot glue used to build the tray molds the teeth and perfectly adapts to the teeth, having enough stiffness to maintain their anatomy and the position of the brackets, but also presenting adequate flexibility to allow removal of the tray avoiding excessive stress over the brackets. In conclusion, the new simplified indirect bonding technique presented here provides a precise placement of brackets on the models, a cheaper way to transfer them to patient, and an easy removal of transfer tray, being a very simple and cost-effective method.

Anthracnose is a foliar and fruit disease caused by Colletotrichum spp. affecting a wide range of crops. Infection occurs early followed by quiescence in fruits, such as in banana, where chemical-based pesticides are used as a dependable fungal control for many years. There is an increasing need for a safe control and as implicated in the Organic Agriculture Act of 2010 (RA 10068) in the Philippines. This scenario drove the use of alternative pest control such as the use of biologicals and natural products. In this study, seven bacteria were isolated from wild honey, produced by Apis mellifera, wherein four (BC2, BC3, BC6 and BC7) were found to be an effective antagonist against Colletotrichum musae in in vitro conditions. These bacteria were identified to belong to the genus Lactobacillus spp. (BC2, BC3, BC7) and Bacillus spp. (BC6) based on sugar utilization tests, morphological and cultural growth in PDPA. For the in vivo test, different dilutions of wild honey were used and it was found out that lower concentrations were effective as biopesticide spray to prevent anthracnose infection. Lastly, we report herewith the first isolation of bacteria with biological control potential from wild honey, and to apply the raw or natural product as biopesticide in postharvest fruits.

This study aimed to determine the gas exchange and the chlorophyll content of green pepper plants under doses and times of application of bio-fertilizers based on manure and enriched organic compost. Two experiments were carried out simultaneously with applications of bio-fertilizers prepared from manure and enriched organic compost, one using cattle manure (CBF) and the other sheep manure (SBF). For these, four doses of biological fertilizers (100, 200, 300 and 400 dm³ ha-1), three application times (0, 30 and 60 days after transplantation - DAT) and absolute control, referring to the absence of fertilization, were used. treatments. were arranged in a randomized block design, totaling 13 treatments. The variables evaluated were: the relative chlorophyll a, b and total content; liquid photosynthesis (A); stomatal conductance (gs); internal CO2 concentration (Ci); instant carboxylation efficiency (iCE - A/Ci); transpiration rate (T); intrinsic water use efficiency (iWUE - A/gs); and water use efficiency (WUE - A/E). Gs, A and T, showed significant effect at 60 DAT with the application of SBF and Ci at 30 DAT with CBF. The dose of 400 dm³ ha-1 of SBF provided greater gas results, and the doses of 200 and 300 dm³ ha-1 of CBF promoted a greater Ci, greater stomatal conductance, greater liquid photosynthesis and better water use efficiency, which results in a greater plant fresh weight at the time of flowering induction.

This study was developed to examine morpho-agronomic traits of 18 sunflower cultivars and identify superior cultivars in terms of grain yield, forage quality, or both, for animal feeding. Twenty-two morpho-agronomic traits related to plant development and architecture; earliness of maturity; grain yield (achenes); dry matter yield; and dry matter content were evaluated. Cultivars Hélio 253, Hélio 358, Embrapa 122, BRS 321, and Hélio 360 showed inflorescence at the final stage. Aguará 4 showed the lowest flowering rate, characterizing it as late-maturing. For grain yield, cultivars Charrua, Olisun 3, BRS 321, Paraíso 103CL, Paraíso 65, Aguará 6, and CF 101 are recommended, as they showed the highest achene yields (average: 1,541.67 to 2,148.81 kg.ha−1, respectively). Cultivars Charrua, Hélio 251, Olisun 3, Hélio 360, Paraíso 55, and Paraíso 103CL exhibited higher dry matter yields (9,550.93 to 11,789.91 kg ha−1) and were thus indicated for forage production. Cultivars Charrua, Olisun 3, BRS 321, Paraíso 103CL, Paraíso 65, Aguará 6, and CF 101 are recommended for grain yield, for the diet of monogastric animals; Charrua, Hélio 251, Olisun 3, Hélio 360, Paraíso 55, and Paraíso 103CL for forage yield, for ruminant feeding; and Charrua, Olisun 3, and Paraíso 103CL for both purposes.

Late pregnancy is accompanied by dramatic changes in the metabolism of sheep, in which the nutrient requirements increase and the metabolic capacity of ewes is under severe stress due to the rapid foetal growth and development of the mammary gland. The blood metabolic profile can be used to monitor these alterations, which can lead to metabolic disorders such as pregnancy toxaemia. However, data available on serum parameters in sheep do not consider physiological state. Therefore, the present study aimed to determine the biochemical reference ranges for pregnant ewes, including serum energy, protein and enzyme-related metabolites. Data from a variety of metabolites were obtained from experiments performed in several institutions and commercial farms using Santa Inês, Dorper, Lacaune, Morada Nova, Bergamacia and Suffolk ewes reared under different conditions (grazing, feedlot, semi-feedlot, collective and/or individual pens, and metabolic cages) from 2006 to 2017. All animals were healthy and without feed restriction. Data from ewes with any clinical manifestations were removed. The metabolic energy profile included data of glucose, cholesterol, triglyceride, high-density lipoprotein, low-density lipoprotein and very-low-density lipoprotein levels; the metabolic protein profile included the metabolites total protein, uric acid, urea, albumin, and creatinine; and the metabolic enzymatic profile included the enzymes aspartate aminotransferase, gamma-glutamyl transferase and alkaline phosphatase. The reference ranges were estimated using the software RefVal 4.11. Dixon’s test was used to identify and remove outliers. The confidence intervals and percentiles were estimated using the nonparametric method of bootstrap when data were not normally distributed. A 95% confidence level was used. The serum biochemical reference ranges for pregnant sheep determined in our study were strongly divergent from those established by one of the most cited books on the topic, especially considering the high serum urea and cholesterol concentrations and low levels of blood glucose observed. Therefore, it is essential to consider physiological status when evaluating the blood metabolic profile of pregnant ewes in order to maintain an adequate nutritional management and to prevent health disorders that may lead to productive and reproductive losses.

This study assessed the potential of termite gut inhabiting bacteria towards bioconversion of cellulosic waste into biofuel. Total seven bacterial isolates from the gut of Heterotermes indicola were isolated. Among all the isolates, HI-1 produced the largest zone upon primary screening. Untreated paper had more cellulose content (73.03%) than acid (0.5%) treated paper that was used as a lignocellulosic substrate for saccharification. Among all the isolates tested, glucose yield (1.08mg/mL) was high for HI-1 isolate. Several factors were considered for optimizing augmented glucose yield (8.57mg/mL) and growth (8.07×108cfu/mL), such as temperature 37°C, pH 4.5, 5% (w/v) substrate concentration, 6 % bacterial inoculum size, agitation 150 rpm with PEG 0.25 % and Ca2+ ions 0.002 g/L. Overall 8-fold increase in glucose yield was achieved. Enzyme activity of HI-1 showed higher endoglucanase 0.29 ± 0.01 (U/mL/min) and exoglucanase 0.15±0.01 (U/mL/min) activity under optimum conditions, mentioned above. temperature 37°C, pH 4.5, substrate concentration 5%, inoculum size 6%, surfactants PEG 0.01%, ions Ca2+(0.002g/L) and agitation (120 rpm). Simultaneous saccharification and fermentation (SSF) of hydrolyzed office paper yielded 5.43mg/mL bioethanol. According to 16S rRNA sequence homology, the bacterial isolate H1 was identified as Alcaligenes faecalis. Bioethanol production from office paper untreated waste proved an effective strategy. Bacteria having natural tendency towards cellulosic waste consumption are promising for bioconversion of cellulosic waste to valuable products.

The present work aims to establish an efficient protocol for in vitro regeneration of peanut (Arachis hypogaea) cultivar L14. The study showed that de-embryonated cotyledon was a suitable explant for shoot multiplication on MS medium containing 4 mg/L BAP. The highest number of shoots per explant obtained after 4 weeks of culture was up to 6.8 shoots. Shoots in vitro were able to produce a large number of approximately 11 roots on MS medium supplemented with 0.5 mg/L NAA. These results will be very useful in establishing an in vitro regeneration protocol for peanut cultivar L14 during gene transfer in the next studies to improve their disease resistance.