Objective To explore the effects of miR-142-3p on CDDP resistance of neuroblastoma cells by targeting high mobility group box-1 protein (HMGB1). Methods The CDDP resistance neuroblastoma cell line SH-SY5Y/CDDP was constructed. And the expression level of miR-142-3p was detected by real-time polymerase chain reaction (PCR). Then the apoptosis level and IC50 value of SH-SY5Y/CDDP were quantified by CCK8 and enzyme-linked immunosorbent assays after a transfection of miR-142-3pmimic. The 3'UTR of HMGB1 was cloned into luciferase reporter vector to verify that miR-142-3p could target HMGB1. The post-transfection expression level of HMGB1 in SH-SY5Y cell was detected by Western-blot. And the IC50 value and apoptosis level of SH-SY5Y/CDDP cells after a knockdown of HMGB1 in SH-SY5Y/CDDP cell line by siRNA. Results The CDDP-resistance cell line SH-SY5Y/CDDP was successfully constructed. The IC50 value of SH-SY5Y/CDDP increased from (4.57±0.65) to (23.53±3.87) μg/ml. And it was 5.15 times higher than parental cell line SH-SY5Y (P<0.01). And the expression level of miR-142-3p decreased remarkably in SH-SY5Y/CDDP cell than SH-SY5Y cell. The apoptosis level of SH-SY5Y/CDDP increased, IC50 value of CDDP decreased(7.02±1.38 vs 24.27±4.13) μg/ml while the expression of miR-142-3p was up-regulated(P<0.05). MiR-142-3p could inhibit the enzymatic activity of luciferase reporter vector of HMGB1 (P<0.01). The expression level of HMGB1 decreased and there was an over-expression of miR-142-3p in SH-SY5Y cell. And the expression levels of HMGB1 and miR-142-3p were negatively correlated in SH-SY5Y cell (P<0.01). The apoptosis level of SH-SY5Y/CDDP increased and IC50 value of CDDP decreased from(24.61±4.07)to(9.65±1.39)and(8.43±1.76) μg/ml respectively after a siRNA knockdown of HMGB1 in SH-SY5Y/CDDP cell line (P<0.05). Conclusions MiR-142-3p can increase the CDDP resistance of neuroblastoma cell by targeting HMGB1. Key words: Neuroblastoma; Drug therapy, combination; Gene expression regulation

Objective To review the current outcomes of bidirectional Glenn （BDG）shunt in the treatment of children with complicated cyanotic congenital heart disease.Methods A total of 103 consecutive patients in high-risk Fontan procedure underwent Glenn shunt at our center from May 2008 to December 2012.The diagnoses included single ventricle （n = 61 ）,transposition of great arteries （TGA,n= 12）,corrected transposition of great arteries （c-TGA,n = 1 5 ）,double outlet of right ventricle （DORV,n = 5 ）,Ebstein anomaly （n =4）,pulmonary atresia with intact ventricular septum （PA/VSD,n = 2 ）and right ventricular hypoplasia （n = 4 ）.There were 1 7 patients with heterotaxy syndrome. A total of 83 patients underwent unilateral operations and the remainder bilaterally.Among them,the procedure was performed with cardiopulmonary bypass （CPB）（n=50） and without （n = 56）.Concomitant procedures included pulmonary artery reconstruction （n = 1 8 ）, atrial septectomy （n = 1 5 ）, pulmonary artery banding （n = 12 ）, correction of total anomalous pulmonary venous connection （TAPVC, n = 6 ）, atrioventricular valve repair （n = 6 ） and atrioventricular valve replacement （n = 2）.Results Two early operative mortalities occurred with a rate of 1 .9%.The mean pulmonary artery pressure was 16.74 ± 2.95 mmHg. And the mean systematic oxygen saturation increased from 68 ± 13% to 84±6% at discharge.The mean follow-up period was 1 .5±1 .2 （0.5-3）years.During the observational period,1 patient died.The procedures included Glenn takedown plus B-T shunt （n = 1 ）and atrioventricular valve replacement （n = 2 ）. Twenty-six patients,considered optimal candidates,underwent Fontan operation.The remainders were well-palliated with an arterial oxygen saturation of 80±1 1 % at rest,cardiac function （NYHA） I-II class and no anastomotic stenosis.Conclusions Glenn shunt is an excellent temporary palliation prior to a Fontan operation for various cyanotic CHDs leading eventually to a single ventricle repair.It is of great importance for preventing an elevation of pulmonary arterial pressure and improving patient outcomes. Key words: Congenital heart disease Single ventricle Cardiac surgical procedures

Objective To identify and quantify total proteins in liver tissue of patients with biliary atresia (BA) by isobaric tags for relative and absolute quantification (iTRAQ) technology to decipher the relationship between significant protein and its prognosis.Methods According to the serum level of total bilirubin (TBIL) in 3 months and native liver survival time (NLST),the patients were divided into 2 groups of good prognosis (3M TBIL≤ 25 μmol/L,NLST＞2 years) and poor prognosis (3M TBIL＞25μmol/L,NLST＜ 1 year) (n =10 each).Liver tissues were collected during Kasai operation.And iTRAQ technique was used to identify and quantify the expression of differential proteins.Then differential proteins were classified by Panther classification system.The results were further validated by Western blot.Results A total of 5177 proteins were identified by mass spectrometry.Among them,323 differential proteins with expression folds of over 1.5 were identified.Among these proteins,as compared with poor prognosis group,131 proteins were up-regulated and 192 proteins down-regulated in good prognosis group.These differential proteins were related with 14 kinds of biological processes (e.g.metabolic and immune system).Conclusions The technique of iTRAQ is effective for comparative proteomic studies.Thus these identified differential proteins will provide further clues for studying the pathogenesis and prognosis of BA. Key words: Biliary atresia; Mifferential proteins; Prognosis

Objective To investigate the expressions of androgen receptors (AR) in testicular and gubernaculum tissues in mice.Methods RNA and protein expressions of AR in testicular and tissues were studied by immtmohistochemistry and RT-PCR.Results AR mRNA was expressed in gubernaculum tissues of neonatal mouse.After birth,the expressions of AR in testis declined at first and then increased.In the group GD (gestation day) 17,GD19,PD (postnatal day,PD) 0,PD3 and PD7,the expression of AR in testes,were 39.49 ± 2.92、33.69 ± 3.46、41.21 ± 2.57、46.47 ± 4.68、55.73 ± 5.55 respectively.At the same time,the expression of AR in gubernaculum increased before birth and then declined.In the group GD17,GD19,PD0,PD3 and PD7,the expression of AR in gubemaculum,respectively,were 40.40 ± 4.08、46.77 ± 6.42、48.71 ± 3.71、45.06 ± 7.02、41.40 ±7.09.Conclusions AR is expressed in both the testis and gubernaculum tissue in neonatal mice,and they show corresponding changes with the aging of newborn mouse. Key words: Testis; Gubernaculum; Receptors,androgen