Atlantic tarpon (Megalops atlanticus) are prized sportfish found through the Gulf of Mexico/America. Atlantic tarpon populations are also considered vulnerable to extinction, and thus many of the recreational fisheries targeting Atlantic tarpon in North America are limited to catch-and-release (CAR). While CAR procedures are common and effective means of protecting recreational sportfish species, it is important to recognize that species-specific traits can impact their efficacy. Here, we sought to explore the importance of Atlantic tarpon air-breathing behaviour in the context of recovery from exercise, which may impact their vulnerability to CAR angling events. A first series of experiments demonstrated that Atlantic tarpon increased air breathing rate following exposure to hypoxia—reinforcing their status as a facultative air-breather—but not following exhaustive exercise. A second series of experiments assessed whether the recovery of biochemical indicators of exhaustive exercise stress in the white muscle and plasma would be impacted by restricted air access during recovery. For fish with access to air, normal patterns of exhaustive exercise were noted in the plasma and white muscle with the exception that haematological parameters were unaffected by exercise. Access to air resulted in no significant differences in recovery profiles at the 1-h time point. Interestingly, exercise resulted in a significant and sustained reduction in red blood cell pH, which coincided with a significant impairment in oxygen binding affinity at higher oxygen partial pressures, possibly explaining why air-breathing behaviour is not beneficial during exercise recovery. Overall, these data suggest that Atlantic tarpon conform to typical patterns of exercise recovery in fishes and that no special consideration are required with respect to CAR angling.

The endangered green sea turtle (Chelonia mydas; hereafter C. mydas) plays a crucial role in maintaining the balance of marine ecosystems. However, its populations are highly vulnerable to various threats, including marine pollution. Rapa Nui (Easter Island), an isolated location in the southeastern Pacific, provides vital foraging habitats for both morphotypes of Pacific C. mydas (black and yellow). In this study, we examined the demographic structure (morphotype, life stage, sex) and health status (based on blood analytes and mercury-Hg concentration) of C. mydas on Rapa Nui during 2018 and 2023. Turtles from various life stages and sexes were observed, with a predominance of yellow morphotype juveniles, likely recently recruited or emerging from brumation. Haematological analyses revealed low levels of several key analytes (e.g. cholesterol, calcium, phosphorus, total protein, globulins), suggesting poor nutritional status, potentially related to the brumation process, limited food availability or poor food quality in the region. Alterations in both red and white blood cell lines, including anaemia and lymphopenia, indicate ongoing inflammatory states and infections, consistent with clinical observations. Rapa Nui turtles exhibited some of the highest blood Hg concentrations globally. Abnormalities in blood profiles, along with correlations between various analytes and blood Hg concentrations, suggest altered immune function and probable renal and liver dysfunction, likely resulting from both natural and anthropogenic sources of this heavy metal. Additionally, a very high body condition index in turtles with carapace lesions suggests a negative impact from human food subsidies in local bays, particularly from high-trophic-level fish, which may also serve as a pathway for Hg accumulation, both for the turtle aggregation and the human population. Our findings underscore the urgent need for long-term mercury monitoring and turtle movement studies to identify pollution sources, inform effective conservation strategies for this endangered species, and address potential public health concerns on this remote Pacific island.

Defining plant ecophysiological responses across natural distributions enables a greater understanding of the niche that plants occupy. Much of the foundational knowledge of species' ecology and responses to environmental change across their distribution is often lacking, particularly for rare and threatened species, exacerbating management and conservation challenges. Combining high-resolution species distribution models (SDMs) with ecophysiological monitoring characterized the spatiotemporal variation in both plant traits and their interactions with their surrounding environment for the range-restricted Aluta quadrata Rye & Trudgen, and a common, co-occurring generalist, Eremophila latrobei subsp. glabra (L.S.Sm.) Chinnock., from the semi-arid Pilbara and Gascoyne region in northwest Western Australia. The plants reflected differences in gas exchange, plant health and plant water relations at sites with contrasting suitability from the SDM, with higher performance measured in the SDM-predicted high-suitability site. Seasonal differences demonstrated the highest variation across ecophysiological traits in both species, with higher performance in the austral wet season across all levels of habitat suitability. The results of this study allow us to effectively describe how plant performance in A. quadrata is distributed across the landscape in contrast to a common, widespread co-occurring species and demonstrate a level of confidence in the habitat suitability modelling derived from the SDM in predicting plant function determined through intensive ecophysiology monitoring programmes. In addition, the findings also provide a baseline approach for future conservation actions, as well as to explore the mechanisms underpinning the short-range endemism arid zone systems.

Thermoregulation is critical for endotherms living in hot, dry conditions, and maintaining optimal core body temperature (Tb) in a changing climate is an increasingly challenging task for mammals. Koalas (Phascolarctos cinereus) have evolved physiological and behavioural strategies to maintain homeostasis and regulate their Tb but are thought to be vulnerable to prolonged heat. We investigated how weather, behaviour and disease influence Tb for wild, free-living koalas during summer in north-west New South Wales. We matched Tb with daily behavioural observations in an ageing population where chlamydial disease is prevalent. Each individual koala had similar Tb rhythms (average Tb = 36.4 ± 0.05°C), but male koalas had higher Tb amplitude and more pronounced daily rhythm than females. Disease disrupted the 24-hr circadian pattern of Tb. Koala Tb increased with ambient temperature (Ta). On the hottest day of the study (maximum Ta = 40.8°C), we recorded the highest (Tb = 40.8°C) but also the lowest (Tb = 32.4°C) Tb ever documented for wild koalas, suggesting that they are more heterothermic than previously recognized. This requires individuals to predict days of extreme Ta from overnight and early morning conditions, adjusting Tb regulation accordingly, and it has never been reported before for koalas. The large diel amplitude and low minimum Tb observed suggest that koalas at our study site are energetically and nutritionally compromised, likely due to their age. Behaviour (i.e. tree hugging and drinking water) was not effective in moderating Tb. These results indicate that Ta and koala Tb are strongly interconnected and reinforce the importance of climate projections for predicting the future persistence of koalas throughout their current distribution. Global climate models forecast that dry, hot weather will continue to escalate and drought events will increase in frequency, duration and severity. This is likely to push koalas and other arboreal folivores towards their thermal limit.

Measurement of cortisol in fish scales is attracting considerable attention as a non-invasive indicator of chronic stress in wild populations. For many fish species of management and conservation interest, extensive scale collections exist that could provide extended records of individual stress responses, by combining cortisol measurements with life history information. However, it is not yet known how well cortisol is preserved in the scale during storage. To investigate the stability of scale cortisol, we accelerated potential degradation by storing scales from an individual farmed Atlantic salmon (Salmo salar) in an oven at 50°C for between 2 and 12 weeks. We found no significant relationship between scale cortisol concentration and either storage time or storage temperature. Cortisol concentrations in scales from the same fish were consistent (18.54–21.82 ng. g−1; coefficient of variation (CV) = 3.6%), indicating that scale cortisol can be reliably quantified, even in scales stored for varying periods of time or under different conditions. We also examined the effects of storage in real time using Atlantic salmon scales that were stored in paper envelopes at room temperature for between 3 and 32 years and found no significant relationship between scale cortisol concentration and storage time. Scale cortisol concentrations ranged from 4.05 to 135.37 ng.g−1 and levels of between-individual variability were high (CV = 61%). Given that scale cortisol does not degrade during long-term storage, historical scale collections and associated data describing fish life histories could potentially be used to develop bioindicators of physiological responses in fish populations. Further research is needed to understand scale cortisol variability and its biological relevance.

Catch-and-release angling exposes fish to challenges that may result in sub-lethal effects or mortality. Lake trout (Salvelinus namaycush) undergo high rates of release because of size-based harvest regulations or voluntary angler behaviour. Here, we examine short-term impairment in lake trout angled during the summer (n = 74) and fall spawning period (n = 33) to inform best practices for angling. Immediately following capture or 0.5h post-capture, fish underwent reflex and barotrauma assessments, and a small blood sample was collected. Fish were also fitted with an externally mounted biologger equipped with depth, temperature and tri-axial acceleration sensors, that was tethered to allow retrieval of the logger after 14min. In the summer, reflex impairment and barotrauma at 0 and 0.5h were significantly correlated. Loss of orientation and bloating were the most observed indicators. Larger fish and those captured at increased depth had higher barotrauma scores, while prolonged fight times decreased the barotrauma score regardless of sampling time. Plasma cortisol, lactate and glucose increased 0.5h after capture, and extracellular and intracellular pH decreased, all signs that angling was inducing a metabolic response. However, no relationships were found between blood indices and mortality (18.9%). The time required to reach maximum depth after release was longer for fish with increased air exposure but shorter for those with longer fight times. During the fall, fish displayed no mortality or reflex impairment. Anal prolapse was the most observed indicator of barotrauma but only observed in females. Blood indices were most altered 0.5h after capture, with increased cortisol values for fish that were female, particularly large or captured at deeper depth. Locomotor activity was highest for males and increased with depth. Together, our findings suggest that the effects of catch-and-release angling may be dependent on several factors, including sex, season and angling depth.

Declining sea ice and increased variability in sea ice dynamics are altering Arctic marine food webs. Changes in sea ice dynamics and prey availability are likely to impact pagophilic (ice-dependent and ice-associated) species, such as thick-billed murres (Uria lomvia), through changes in foraging behaviour and foraging success. At the same time, extrinsic factors, such as chick demand, and intrinsic factors, such as sex, are also likely to influence foraging behaviour and foraging success of adult murres. Here, we use 3 years of data (2017-2019) to examine the impacts of environmental conditions (sea ice concentration and sea surface temperature), sex and chick age (as a proxy for chick demand) on foraging and diving behaviour (measured via biologgers), energy expenditure (estimated from activity budgets) and foraging success (measured via nutritional biomarkers) of thick-billed murres during the incubation and chick-rearing stages at Coats Island, Nunavut. Murres only exhibited foraging flexibility to environmental conditions during incubation, which is also the only stage when ice was present. When more ice was present, foraging effort increased, murres foraged farther and made deeper dives, where murres making deeper dives had higher foraging success (greater relative change in mass). During incubation, murre behaviour was also influenced by sex of the individual, where males made more and shorter trips and more dives. During chick-rearing, murre behaviour was influenced primarily by the sex of the individual and chick age. Males made shallower dives and fewer dive bouts per day, and more dives. Birds made longer, deeper dives as chicks aged, likely representing increased intra-specific competition for prey throughout the season. Our results suggest variation in sea ice concentration does impact foraging success of murres; however, sex-specific foraging strategies may help buffer colony breeding success from variability in sea ice concentration.

The study of repeatability in behaviour and activity level can be used to evaluate inter-individual differences, which are fundamental to assess the resilience of populations to environmental variation. Previous work on repeatability in wild fish populations has largely been based on acoustic telemetry or mark-and-recapture and has revealed repeatable activity patterns over relatively long periods in a number of species. Although accelerometry is a promising tool for investigating the swimming activity of fish in the wild, little is known about the repeatability of accelerometry-based traits in wild fish. Here, we used external accelerometers to investigate the swimming activity of the Patagonian grouper Acanthistius patachonicus, a rocky-reef fish with high site fidelity, which ensures a high recapture rate of accelerometer tags. Accelerometry was used to investigate the short-term repeatability of a number of activity traits, including swimming, hovering, daily median tailbeat frequency, percentage of high tailbeat frequency and total number of tailbeats at different times of the year. We found that all of the variables are repeatable over the daily scale and four out of five variables are repeatable over weekly periods. Overall, our work suggests that these traits are individual-specific for the short time period investigated. In addition, the percentage of time spent in swimming and hovering was greater in the warm season compared to the cold season, suggesting higher activity levels related to higher temperatures. These results suggest that activity traits related to swimming are repeatable and likely related to the physiological state of each individual. Finally, our work shows that accelerometry can be considered a valuable tool to explore inter-individual differences with potential applications for assessing the resilience of wild populations.

This is an Editor’s Note regarding: Tusheema Dutta, Uttpal Anand, Suchismita Chatterjee Saha, Abhijit Bhagwan Mane, Dorairaj Arvind Prasanth, Ramesh Kandimalla, Jarosław Proćków, Abhijit Dey, Advancingurban ethnopharmacology: a modern concept of sustainability, conservation and cross-cultural adaptations of medicinal plant lore in the urban environment, Conservation Physiology, Volume 9, Issue 1, 2021, coab073, https://doi.org/10.1093/conphys/coab073

Marine heatwaves (MHWs) are increasing in frequency and intensity. Coral reefs are particularly susceptible to MHWs, which cause mass coral bleaching and mortality. However, little is known about how MHWs affect coral reef fishes. Here, we investigated how MHWs affect the physiology of a coral reef mesopredator, Lutjanus carponotatus. Specifically, we exposed mature adults to two different MHW intensities, +1°C (29.5°C) and + 2°C (30.5°C) and measured physiological performance at 2 and 4 weeks of exposure and at 2 weeks post-exposure. At these time points, we measured oxygen consumption at rest and after a simulated fishing capture event, recovery time, excess post-exercise oxygen consumption (EPOC) and associated biochemical markers in the blood (baseline lactate, post-capture lactate, glucose, haemoglobin levels and haematocrit proportion). We found that 2 weeks of exposure to MHW conditions increased resting oxygen consumption (+1°C = 23%, +2°C = 37%), recovery time (+1°C = 62%, +2°C = 77%), EPOC (+1°C = 50%, +2°C = 68%), baseline lactate (+1°C = 27%, +2°C = 28%), post-capture lactate (+1°C = 62%, +2°C = 109%) and haemoglobin levels (+1°C = 13%, +2°C = 28%). This pattern was maintained at 4 weeks of exposure except for post-capture lactate which was reduced (+1°C = -37%, +2°C = 27%). In combination, these results suggest a greater reliance on anaerobic glycolysis to maintain homeostasis in MHW conditions. At 2 weeks post-exposure, when compared to control fish, we found that capture oxygen consumption was increased (+1°C = 25%, +2°C = 26%), recovery rate was increased (+2°C = 38%) and haemoglobin was still higher (+1°C = 15%, +2°C = 21%). These results show that MHW conditions have direct physiological demands on adult coral reef snapper and ecologically relevant residual effects can last for at least 2 weeks post-MHW; however, individuals appear to recover from the negative effects experienced during the MHW. This provides new insight into the effects of MHWs on the physiological performance of coral reef fishes.