All Research Papers Published In Enzyme and Microbial Technology

Research Article
5
10.1016/j.enzmictec.2021.109918

Functional expression and extracellular secretion of Clostridium thermocellum Cel48S cellulase in Escherichia coli via the signal recognition particle-dependent translocation pathway

Sep 15, 2021
Enzyme and Microbial Technology
Hyun-Jae Lee + 4 more +4

Research Article
9
10.1016/j.enzmictec.2021.109915

Catalytically active inclusion bodies (CatIBs) induced by terminally attached self-assembling coiled-coil domains: To enhance the stability of (R)-hydroxynitrile lyase

Sep 09, 2021
Enzyme and Microbial Technology
Xiaolin Pei + 5 more +5

Research Article
19
10.1016/j.enzmictec.2021.109916

Design of a PL18 alginate lyase with flexible loops and broader entrance to enhance the activity and thermostability

Sep 09, 2021
Enzyme and Microbial Technology
Bingmei Su + 5 more +5

Research Article
5
10.1016/j.enzmictec.2021.109899

Bioanalytical insight into the life of microbial populations: A chemical monitoring of ureolytic bacteria growth

Sep 03, 2021
Enzyme and Microbial Technology
Justyna Bzura + 2 more +2

In this publication an alternative approach to investigations of bacterial growth is proposed. Contrary to the conventional physical methods it is based on enzyme activity detection. The procedure for real-time and on-line monitoring of microbial ureolytic activity (applied as a model experimental biosystem) in the flow analysis format is presented. The developed fully-mechanized bioanalytical flow system is composed of solenoid micropumps and microvalves actuated by Arduino microcontroller. The photometric detection based on Nessler reaction is performed using dedicated flow-through optoelectronic detector made of paired light emitting diodes. The developed bioanalytical system allows discrete assaying of microbial urease in the wide range of activity up to 5.4 U mL−1 with detection limit below 0.44 U mL−1, a high sensitivity in the linear range of response (up to 200 mV U−1 mL and relatively high throughput (9 detection per hour). The proposed differential procedure of measurements (i.e. a difference between peaks register for sample with and without external addition of urea is treated as an analytical signal) allows elimination of interfering effects from substrate and products of biocatalysed reaction as well as other components of medium used for microbial growth. The developed bioanalytical system was successfully applied for the control of growth of urease-positive bacteria strains (Proteus vulgaris, Klebsiella pneumoniae and Paracoccus yeei) including examination of effects from various microbial cultivation conditions like temperature, composition of culture medium and amount of substrate required for induction of bacterial enzymatic activity. The developed bioanalytical flow system can be applied for metabolic activity-based estimation of parameters of lag and log phases of microbial growth as well as for detection of decline phase.

Research Article
1
10.1016/j.enzmictec.2021.109881

Identification of methionine adenosyltransferase with high diastereoselectivity for biocatalytic synthesis of (S)-S-adenosyl-l-methionine and exploring its relationship with fluorinated biosynthetic pathway

Aug 08, 2021
Enzyme and Microbial Technology
Siyu Ren + 2 more +2

Research Article
9
10.1016/j.enzmictec.2021.109887

Assembling mini-xylanosomes with Clostridium thermocellum XynA, and their properties in lignocellulose deconstruction

Aug 02, 2021
Enzyme and Microbial Technology
Pedro R.v Hamann + 3 more +3

Research Article
11
10.1016/j.enzmictec.2021.109857

Improving the catalytic behaviors of Lactobacillus-derived fructansucrases by truncation strategies

Jun 24, 2021
Enzyme and Microbial Technology
Dawei Ni + 6 more +6

Research Article
13
10.1016/j.enzmictec.2021.109855

Triple signal amplification strategy for the ultrasensitive electrochemical detection of human papillomavirus 16 E6/E7 mRNA

Jun 19, 2021
Enzyme and Microbial Technology
Nanfei Yang + 7 more +7

Research Article
12
10.1016/j.enzmictec.2021.109853

Characterization of polyphosphate kinases for the synthesis of GSH with ATP regeneration from AMP

Jun 12, 2021
Enzyme and Microbial Technology
Caixia Cui + 4 more +4

Research Article
65
10.1016/j.enzmictec.2021.109836

A review on enzyme-producing lactobacilli associated with the human digestive process: From metabolism to application

Jun 11, 2021
Enzyme and Microbial Technology
Bruna L Maske + 9 more +9