This study aimed to improve age prediction in a Danish cohort of 150 individuals by in-depth analyses of DNA methylation sequencing data. In previous work, 11 well-known age-associated CpGs located on 10 PCR amplicons associated with 10 distinct genes were selected, and a support vector machine model with a polynomial function (SVMp) was developed for age estimation in blood. The 10 amplicons were PCR-amplified after bisulphite conversion and the methylation levels were assessed by massively parallel sequencing. The 10 amplicons contained a total of 73 CpG sites, of which 63 CpG sites were shown to be medium to highly associated with age in this work (Pearson correlation coefficient, |r| > 0.3). More than 70 000 SVMp models with various combinations of the 63 CpGs were tested to identify the model with the lowest mean absolute error (MAE) and root mean square error (RMSE) in training and test datasets from the Danish cohort. A final model with 34 CpGs (SVMp-34CpG) was selected. The SVMp-34CpG model showed considerable improvement over many other forensic prediction models and achieved mean absolute errors of 1.64 years and 1.76 years for the training and test datasets, respectively. The SVMp-34CpG model indicated robust generalization and minimal prediction bias across the 18–50 years age range (MAE: 1.47 years), although slightly higher errors were observed for individuals aged 50–70 years (MAE: 2.62 years). This was most likely due to epigenetic drift, together with the small number of individuals analysed in this age group. Compared to the previous 11-CpG model, the SVMp-34CpG model showed superior accuracy in the Danish cohort by predicting the age of 91% of the individuals within 4 years of their actual age, versus 68% for the previous model.

Forensic science explores scientific knowledge to clarify legal and legal issues. Since I started in this area of knowledge in mid-2007, my priority has been to create a centre of reference and excellence that would advance forensic science, an area sometimes based on self-taught practices. Along this pathway, I always considered that we were dealing with an articulated set of “Forensic Sciences” and not with a single “Forensic Science”, but I was, at least partially, wrong! This work discusses the competencies of the different forensic subspecialties, seeking to be the starting point for reformulating forensic teaching, research, and public and private forensic activity. It also highlights the concern of offering a certain level of professionalization to forensic science. For this reflection, it was important to follow a path that had its foundations in the study of the historical roots of forensic science, namely the first Portuguese forensic case, to study the revolutionary scientific contribution triggered by the “CSI effect”, to understand in depth the competencies of each of the forensic subspecialties and bring this knowledge to specialized pre- and postgraduate training, to promote innovation through peer review of empirical forensic practices to increase the scientific evidence, the development of certification mechanisms for forensic experts and their respective code of ethics, and to open the doors for artificial intelligence to assist the courts and the opportunity that forensic science represents in the development of other areas of health sciences, life, and social behaviour. Thus, it was born, very unconsciously, a scientific project for the transdisciplinary reformulation of forensic activity, but over time, it allowed me to realize that we can have specialists trained in all its dimensions and plenitude. The pillars for the modernization of this science and, ultimately, the law, which, together with education and health, are the three main pillars of society. Not punishing innocent people, leaving criminals unharmed through scientific evidence, seeking justice, and repairing the damage is the supreme spirit of Forensic Science. Our One Forensics model is now being considered to be implemented by other countries.

Age estimation is a critical aspect of forensic entomology, especially in the examination of pupae. The use of proteins as a means for age identification shows great promise. In this study, proteomic techniques were employed to investigate differentially expressed proteins (DEPs) during the intrapuparial stage of Chrysomya megacephala. Specimens were sampled at four distinct time points: 0 h (Group A), 24 h (Group B), 48 h (Group C), and 72 h (Group D). Our analysis uncovered 56 DEPs between Groups B and A, 116 DEPs between Groups C and A, and a total of 152 DEPs between Groups D and A. These DEPs were categorized into nine clusters based on their expression patterns. Cluster 1 exhibited an increasing trend in protein expression, while Cluster 4 displayed the opposite pattern. Clusters 2, 6, and 9 showed an initial rise followed by a decline, whereas Cluster 3 demonstrated the reverse trend. Cluster 8 indicated an initial rise, a subsequent drop, and another rise, while Cluster 7 showed an initial decrease, followed by an increase and a minor decrease. Notably, the C-type lectin domain-containing (CTLD) protein and Failed axon connections (Fax) protein consistently displayed an upward trend. These two DEPs were selected for validation using the parallel reaction monitoring technique–targeted proteomics, confirming the trends observed in the initial analysis. In summary, this study highlights the potential of using proteins as reliable biomarkers for estimating pupal age.

Thermochromic ink disappears with heat but is reversible with cold.UV techniques and IR photography serve to make thermochromic ink visible.Initially use oblique white light opposite to the angle of observation to make strokes visible.

With various calls in the forensic scientific literature for pattern-matching disciplines to adopt evaluative reporting, which is also known as the likelihood ratio or logical approach, practitioners used to reporting evidence under a more traditional paradigm may have a level of apprehension and face a steep learning curve. There may be doubts about the benefits of evaluative reporting, when it applies and when it does not, what it actually entails, its validity, and how to explain it in court. These questions and more are answered in this comprehensive collection of frequently asked questions on evaluative reporting. While the focus is on providing context for forensic handwriting examiners, many of the questions and answers are general in nature and the concepts can be applied to other disciplines.

Traditional radiocarbon dating and bomb-pulse dating are essential tools to establish the archaeological relevance or medico-legal significance of human skeletal remains.Bomb-pulse dating enables assessment of an individual's years of birth and death.Bomb-pulse dating helps to narrow down the pool of candidates for identification.Radiocarbon analysis provides mutual benefits for archaeologists, forensic anthropologists, and the criminal justice system.

[This corrects the article DOI: 10.1093/fsr/owad046.].

Identification of unidentified remains involves a comparison of ante- and postmortem features using biological identifiers. Anthropological identifiers, referred to by International Criminal Police Organization (INTERPOL) as secondary identifiers, have often been judged less reliable than DNA, fingerprints and dental records (referred to as primary identifiers). However, anthropological identifiers have been proven as discriminatory as the primary sources in many instances, and play a decisive role in positive identification. To guarantee better use of anthropological identifiers, it is not only essential to develop standard protocols and statistical frameworks, but also to test different identification approaches in cases from daily practice. Evidence of skeletal antemortem trauma can be a valuable aid in the identification process, especially if the exact type of traumatic event causing the injury is identified. Here, we present a case in which the combination of anthropological analysis and imaging confirmed an interesting and unique sequence of antemortem traumatic events in incomplete skeletal remains. The remains were assumed to pertain to an individual who went missing several years earlier, and whose medical records revealed a unique history of trauma to the right femur. The individual had sustained a fracture due to a fall from a high height followed, 10 years after the primary trauma, by a gunshot wound to the same bone; both treated by intramedullary nail fixation. While the anthropological analysis matched the biological profile of the missing individual and identified a healed defect to the right femur compatible with a gunshot wound, the radiological examination indicated that the bone underwent three surgical procedures on different occasions. Radiological examination also identified a pre-existing healed fracture adjacent to the gunshot defect. In addition to presenting the identification process in this specific case, this article discusses the difficulties in antemortem trauma interpretation, importance of combining macroscopic and radiological analysis to aid the reconstruction of previous traumatic events and mechanisms of injury from healed fractures that can play important roles in forensic human identification.

Human skeletal remains were recovered from Sandy Point, Victoria.The absence of circumstantial information and the poor preservation (i.e. skeletonized) of the remains precluded the formation of an identification hypothesis, rendering the identification process complex.Only through the integration of anthropology, odontology, molecular biology, radiocarbon dating, historical research, and genealogy were the remains able to be identified as Mr. Christopher Luke Moore, who drowned in 1928.Human identification is a process that necessarily requires a multidisciplinary and collaborative approach.