sion="1.0" encoding="utf-8"?> FP Functional Plant Biology Funct. Plant Biol. 1445-4408 1445-4416 CSIRO Publishing 36 Gardiner Road Clayton VIC 3168 Australia FP22050 10.1071/FP22050 Foreword Advanced technologies in studying plant photosynthesis: principles and applications A. Zavafer et al . https://orcid.org/0000-0002-8905-1618 Zavafer Alonso A Fan Dayong B * https://orcid.org/0000-0001-8150-9535 Murakami Keach C Handling Editor Shabala Sergey Plant Science Division, Research School of Biology, The Australian National University, Canberra, ACT 2001, Australia. Hokkaido Agricultural Research Center (HARC), National Agriculture and Food Research Organization (NARO), 1 Hitsujigaoka, Toyohira, Sapporo 062-8555, Japan. College of Forestry, Beijing Forestry, University, Beijing 100083, China. * Correspondence to: Dayong Fan Hokkaido Agricultural Research Center (HARC), National Agriculture and Food Research Organization (NARO), 1 Hitsujigaoka, Toyohira, Sapporo 062-8555, Japan Email: dayong73fan@163.com 9 May 2022 49 6 Special Issue i iii 9 May 2022 Published: 9 May 2022 © 2022 The Author(s) (or their employer(s)). Published by CSIRO Publishing 2022 The Authors The foreword to this special issue on the advanced technologies in studying photosynthesis focuses on the main contributions of Fred Chow, one of the key Australian scientists studying light reactions in plants.

Starch is a major component of the endosperm, directly determining grain yield and quality. Although the key enzymes of starch synthesis have been identified and characterised, the regulatory mechanisms remain unclear. In this study, we identified the novel maize STARCH SYNTHESIS REGULATING PROTEIN1 (ZmSSRP1 ), which encodes a typical carbohydrate-binding module 48 (CBM48) protein. Expression analysis revealed that ZmSSRP1 was highly expressed in the maize endosperm, while transient expression in maize leaf protoplasts showed localisation in the plastids, dependent on the N-terminal transit peptide. In addition, overexpression of ZmSSRP1 in rice resulted in a decrease in grain thickness and the 1000-grain weight, as well as affecting the starch content and structure of the rice endosperm. The physicochemical properties of starch in the rice endosperm were also altered compared with the wild-type seeds. Real-time quantitative reverse transcription polymerase chain reaction (qRT-PCR) was subsequently performed to determine the expression of starch synthesis-related genes, revealing upregulation of mRNA expression of most genes in the transgenic compared with wild-type lines. Collectively, these findings suggest that ZmSSRP1 acts as a potential regulator of starch synthesis, providing new insight for molecular breeding of high-yielding high-quality maize.

Tolerance to freezing and seedling diseases caused by Microdochium spp. is an essential trait for the wintering of triticale (×Triticosecale Wittmack) and other cereals. Preceding multi-year studies indicate that after long-term exposure to the low temperature, cereal seedlings acquire a genotype-dependent cross-tolerance to other subsequent stresses. This paper presents the first non-gel protein profiling performed via high performance liquid chromatography coupled with Mass Spectrometry as well as Fourier Transform-Raman spectroscopy measurements performed directly on leaves of triticale seedlings growing under different conditions. The research used doubled haploid lines selected from the mapping population, with extreme tolerance/susceptibility to freezing and M. nivale infection. These non-targeted methods led to the detection of twenty two proteins cold-accumulated in the most tolerant seedlings in relation to susceptible ones, classified as involved in protein biosynthesis, response to different stimuli, energy balancing, oxidative stress response, protein modification, membrane structure and anthocyanin synthesis. Additionally, in seedlings of the most freezing- and M. nivale -tolerant line, cold-hardening caused decrease of the carotenoid and chlorophyll content. Moreover, a decrease in the band intensity typical for carbohydrates as well as an increase in the band intensity characteristic for protein compounds were detected. Both studied lines revealed a different answer to stress in the characteristics of phenolic components.

Transcriptomic analyses are needful tools to gain insight into the molecular mechanisms underlying plant responses to abiotic stresses. The aim of this study was to identify key genes differentially regulated in response to chilling stress in various plant species with different levels of tolerance to low temperatures. A meta-analysis was performed using the RNA-Seq data of published studies whose experimental conditions were comparable. The results confirmed the importance of ethylene in the hormonal cross-talk modulating the defensive responses against chilling stress, especially in sensitive species. The transcriptomic activity of five Ethylene Response Factors genes and a REDOX Responsive Transcription Factor 1 involved in hormone-related pathways belonging to ethylene metabolism and signal transduction were induced. Transcription activity of two genes encoding for heat shock factors was enhanced, together with various genes associated with developmental processes. Several transcription factor families showed to be commonly induced between different plant species. Protein-protein interaction networks highlighted the role of the photosystems I and II, as well as genes encoding for HSF and WRKY transcription factors. A model of gene regulatory network underlying plant responses to chilling stress was developed, allowing the delivery of new candidate genes for genetic improvement of crops towards low temperatures tolerance.

Calcium signals serve an important function as secondary messengers between cells in various biological processes due to their robust homeostatic mechanism, maintaining an intracellular free Ca2+ concentration. Plant growth, development, and biotic and abiotic stress are all regulated by Ca2+ signals. Ca2+ binding proteins decode and convey the messages encoded by Ca2+ ions. In the presence of high quantities of Mg2+ and monovalent cations, such sensors bind to Ca2+ ions and modify their conformation in a Ca2+ -dependent manner. Calcium-dependent protein kinases (CPKs), calmodulins (CaMs), and calcineurin B-like proteins are all calcium sensors (CBLs). To transmit Ca2+ signals, CPKs, CBLs, and CaMs interact with target proteins and regulate the expression of their genes. These target proteins may be protein kinases, metabolic enzymes, or cytoskeletal-associated proteins. Beyond its role in plant nutrition as a macroelement and its involvement in the plant cell wall structure, calcium modulates many aspects of development, growth and adaptation to environmental constraints such as drought, salinity and osmotic stresses. This review summarises current knowledge on calcium sensors in plant responses to osmotic stress signalling.

Severe water stress is responsible for reducing plant growth and reproduction. This study aimed to evaluate the physiological and biochemical mechanisms associated with the tolerance of two genotipes of bahiagrass (Paspalum notatum Flügge) with different ploidy level to water deficit and flooding at the reproductive stage. Photosynthetic performance of diploid and tetraploid plants was not affected by flooding. In contrast, the water deficit decreased stomatal conductance, increased leaf temperature, and resulted in a decrease in the assimilation rate of the two genotypes. Despite the greater activities of antioxidant enzymes, flooded roots accumulated hydrogen peroxide and malondialdehyde. Roots of plants exposed to water deficit maintained an accumulation of biomass similar to that of control plants; however, with higher levels of total phenol content, total soluble sugars and proline. Diploid plants subjected to flooding had more inflorescences, however, the drought reduced the total number of filled florets per plant. Less starch degradation allows the maintenance and recovery of biomass in the tetraploid genotype, which allows it to maintain its reproductive performance even under drought conditions. Overall, the synthesis of osmoprotectants and activation of antioxidant machinery are important strategies in the tolerance of bahiagrass to water stress at the reproductive stage.

Functional Plant Biology is an international journal of plant function publishing high quality research papers in all areas of plant physiology, applied agricultural research and pure molecular biology

Nitric oxide (NO) and hydrogen sulfide (H2 S) are signalling molecules that regulate adventitious rooting in plants. However, little is known about the cross-talk between NO and H2 S during adventitious rooting. Tomato (Solanum lycopersicum L.) explants were used to investigate the roles of and relationships between NO and H2 S during rooting. Effects of the NO donor sodium nitroprusside (SNP) and the H2 S donor sodium hydrosulfide (NaHS) on adventitious rooting were dose-dependent, and the greatest biological responses were observed under 25μM SNP and 50μM NaHS. The positive effect of NaHS was reversed by the NO scavenger 2-(4-carboxy-2-phenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide (cPTIO), indicating that the H2 S-induced response was partially NO-dependent. Peroxidase (POD), polyphenol oxidase (PPO), and superoxide dismutase (SOD) activities significantly increased by SNP and NaHS treatment, and indoleacetic acid oxidase (IAAO) activity and the O2 - and H2 O2 content significantly decreased by SNP and NaHS treatment. SNP and NaHS treatment also increased the content of soluble sugar and protein and indole-3-acetic acid (IAA). cPTIO significantly mitigated the increases in POD, PPO and SOD activity and soluble sugar, protein and IAA content induced by NaHS. SNP and NaHS upregulated the expression of auxin-related genes (ARF4 and ARF16 ), cell cycle-related genes (CYCD3 , CYCA3 and CDKA1 ), and antioxidant-related genes (TPX2 , SOD and POD ); whereas cPTIO significantly inhibited the increase in the expression of these genes induced by NaHS. Overall, these results show that NO may be involved in H2 S-induced adventitious rooting by regulating the activity of rooting-related enzymes, the expression of related genes, and the content of various nutrients.

Plants take up nitrogen (N) both day and night. The diurnal variation in N uptake results from interactions between aboveground and belowground tissues. We examined the long-term effects of interrupted N supply (day only or night only) under hydroponic conditions to test whether plant acclimatisation response to the interrupted N supply differs by day or night. Seedlings experienced 32 days under daytime-fed (DF), night-time-fed (NF), or continuous (CT) N supply. The root N uptake rate (NUR) differed between DF and NF from day 3 of treatment, after which NUR was significantly increased (by up to 82%) in DF and NF plants. The increased NUR during each half-day did not fully compensate for lost access to N during the other half-day, resulting in lower N accumulation by the end of the treatment. The reduction was smaller in DF plants than NF plants. The underlying mechanism of diurnal variation of N uptake is discussed in terms of transpiration demand and gene expression in roots.

The current hypotheses for the mechanisms of photosystem II (PSII) photodamage in vivo remain split on the primary damage site. However, most researchers have considered that PSII is inhibited by a sole mechanism and that the photoinhibited PSII consists of one population. In this perspective, we propose 'the mixed population hypothesis', in which there are four PSII populations: PSII with active/inactive Mn4 CaO5 oxygen-evolving complex respectively with functional/damaged primary quinone (QA ) reduction activity. This hypothesis provides a new insight into not only the PSII photoinhibition/photoprotection studies but also the repair process. We discuss our new data implying that the repair rate differs in the respective PSII populations.