The darkness of the kernel and the lack of simultaneous ripening of the mesocarp and endocarp are among the most significant challenges in Iranian walnut orchards. The mentioned problems appear more in areas with hot and humid summers. The research focused on the quality of the kernel in terms of colour and marketability, as well as the dry matter of the kernels. Additionally, the ease of mesocarp separation from endocarp was examined. Experimental trials were designed with 27 treatment combinations to address the mentioned issues using varying concentrations of 2-chloroethylphosphonic acid (ethephon) at different harvest dates. In this study, using 1 920 ppm of ethephon on August 22nd resulted in the best quality walnut kernels and mesocarp. Researchers in this project successfully achieved the highest quality of walnut kernels and nuts in a region where over 90% of the harvest consisted of dark-kernel walnuts.

Optimum nutrition is essential for quality cut flower production and for improving soil health. The study aims to evaluate the interaction between bio-organic and inorganic nutrient sources in enhancing alstroemeria (<i>Alstroemeria hybrida</i> L.) cut flower production and soil health for sustainable cultivation practice. Randomised block methodology involving 20 treatment combinations of inorganic fertilisers (NPK) and biostimulants (Panchgavya and Jeevamrit) applied at varying concentrations. Significant outcomes emerged from the combined influence of inorganic and organic sources of nutrients. The application of the 100% recommended dose of fertiliser (RDF) with 75 mL of Panchgavya resulted in substantial improvements in alstroemeria growth parameters, including plant height (7.8%), early flowering (13.08%), flower diameter (20.03%) and the number of flowering stems (25.3%) over the control (100% RDF). Plant spread (24.1%) and number of florets/stem (26.6%) were improved with the application of 100% RDF with 50 mL of Panchgavya when compared with the control. Soil nutrient content, i.e. available nitrogen (11.5%), phosphorus (28.7%), potassium (13.8%) and microbial populations, i.e. fungal (35.4%) and bacterial (28.2%) colonies also exhibited noteworthy enhancements with the application of 100% RDF with 75 mL of Panchgavya over the control. The study concludes that the application of 100% RDF with 75 mL of Panchgavya increased quality cut flower production in alstroemeria.

Stem rot disease poses a significant challenge in passion fruit production, necessitating the identification of resistant genes for the development of stem rot resistant varieties. In this study, we conducted artificial inoculation of <i>Fusarium solani</i> on leaves of two passion fruit varieties, ‘Huangjinguo’ and ‘Ziguo 7’. Leaf samples were collected at 0 h, 24 h, and 48 h post-inoculation for RNA-sequencing (RNA-seq) analysis, and 3 370, 4 464, and 3 974 differentially expressed genes (DEGs) were identified at these stages. Gene Ontology (GO) analysis revealed associations with functions such as response to reactive oxygen species (ROS), response to hydrogen peroxide, and protein complex oligomerisation. Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis highlighted the enrichment of DEGs in the phenylpropanoid biosynthesis pathway, including genes such as <i>ZX.06G0025070</i>,<i> ZX.01G0064640</i>,<i> ZX.04G0011040</i>,<i> ZX.05G0011380</i>, all implicated in lignin biosynthesis. Weighted gene co-expression network analysis (WGCNA) identified three modules significantly associated with passion fruit stem rot resistance. Network analysis highlighted<i> ZX.08G0013660</i> as the gene with the highest connectivity in these modules, featuring a leucine-rich repeat domain. Reverse transcription quantitative real-time polymerase chain reaction (RT-qPCR) analysis further validated <i>ZX.08G0013660</i> and other genes as potential candidates for passion fruit stem rot resistance. Overall, genes related to ROS, phenylpropanoid biosynthesis and leucine-rich repeat domain protein likely play critical roles in passion fruit stem rot resistance. This study provides new insights for breeding passion fruit varieties resistant to stem rot disease.

The availability of a broad variety of cultivars in many ornamental species has increased recently, in particular for attractive annuals, which are valued commercially for their use as cut flowers, potted plants, loose flowers, and in landscape gardening. The breeding of ornamental plants in the current scenario is a challenging endeavour with constantly evolving new obstacles. Modern genomic technologies provide prospects for improved precision breeding and selection for characteristics that are more difficult to determine. Traditionally, ornamental breeding has been focused on increasing resistance to biotic or abiotic stress, novelty, yield, and quality. However, accomplishing these objectives necessitates tedious cross-breeding, and exact breeding methods have been noticed to be not applied constantly. Though the purposes of ornamental crop breeding may vary, the process generally does not differ from the breeding of other crops. Furthermore, vegetatively propagated ornamentals constitute most of the crops. The expanding interest in ornamental crops that are produced by modern crop breeding methods such as genome editing, chromosome manipulation, molecular marker-assisted breeding, mutation breeding, and exploiting somaclonal variations, particularly in relation to altering desirable plant features and producing new ornamental traits of the crops which is the main objective of crop improvement practices. Hence, it has become obligatory to evaluate the current state of any technology created following an in-depth study carried out by several research organisations.

Crops belonging to the Solanaceae family, including potato, tomato, pepper, and tobacco possess considerable economic importance worldwide. However, their production is continuously under threat from plant pathogens. Farmers typically rely on resistant cultivars carrying one or several disease resistance (R) genes introduced through conventional breeding. Over time, a competitive host-pathogen coevolution can lead to major resistance breakdown. Genome editing is a significant research tool and avenue for the genetic improvement of crop species, as it enables the precise introduction of targeted genetic changes. This technology has been successfully used in various food crops, including those belonging to the Solanaceae family. The advent of the CRISPR/Cas9 genome editing system allows the rapid knockout of desirable genes. Plant pathogens often exploit host genes known as susceptibility (S) genes to facilitate their proliferation. Inactivation of these S genes may reduce the pathogen’s ability to infect plants and confer durable and broad-spectrum resistance. This review provides an overview of the current application of CRISPR/Cas9 to disrupt the S genes for the development of disease-resistant solanaceous crops. The technological limitations and potential strategies for overcoming these challenges are discussed.

This study was conducted to determine the effects of phosphite and phosphate fertilisers on broccoli’s yield, growth, and nutritional status (<i>Brassica oleracea</i> L. var. <i>italica</i> cv. ‘Fiorentino’). In factorial combinations, experiments were conducted at three pH levels (5.5, 7.0, and 8.5) and three phosphorus source levels [Pi (phosphate), Phi (phosphite), and Pi + Phi]. Phi had a statistically significant negative effect on yield, producing immature and button-like flower heads. Decreases in chlorophyll index, Fv/Fm [ratio of variable fluorescence (Fv) to maximum fluorescence (Fm)], and leaf area were observed using the Phi treatment. These results indicate that fertilisation with Phi alone did not affect plant growth and yield. P (phosphorus) and K (potassium) concentrations in plants were increased by root fertilisation with Phi (floating system), but did not affect growth characteristics; increased Pi uptake in Phi-treated plants increased P and K concentrations in the sink source, resulting in reduced growth, phytotoxicity, and no head formation. While it may appear that Phi is upsetting the balance of solution nutrients and stressing the plants, the Phi-induced stress condition was identified by measuring proline levels and electrolyte leakage. Thus, phosphite could not be used as a P source for plants. However, Phi could be used as a plant nutrient source combined with conventional Pi fertilisers.

In the study, the effects of 0, 100, 200 and 400 ppm spray applications of GA3 on root and above-ground parts of ‘Jan Reus’, ‘Bloody Mary’ and ‘Yokohama’ tulip cultivars were determined. According to the results, on the development of plant upper part properties, 200 ppm in ‘Jan Reus’ and ‘Yokohama’ and 100 ppm in ‘Bloody Mary’ were the most effective applications. Again, the least increases in upper part properties were obtained from the control plants in ‘Jan Reus’ and ‘Bloody Mary’, and from the 100 ppm application in ‘Yokohama’. While 100 ppm was the most effective application in ‘Jan Reus’ and ‘Yokohama’ in terms of root development, the effect of the applications in ‘Bloody Mary’ was lower than the control. The application that least increased root development was determined as 400 ppm in ‘Jan Reus’ and 200 ppm in ‘Bloody Mary’ and ‘Yokohama’. Root growth was found to be negatively related to GA3 content. High GA3 is thought to negatively affect overall root growth, possibly by suppressing the effect of auxin. It is assumed that GA3 produced by the plant itself may be sufficient for root development or may be effective at much lower dose applications. On the other hand, it has been determined that the effects of GA3 application vary depending on the variety. According to these results, it is recommended to apply it at a dose of 200 ppm to ensure flowering by providing cooling in tulip cultivars and cut flower cultivation. High doses of GA3 are not recommended for root development.

Exogenous polyamines can elevate postharvest fruit cold resistance and alleviate cold injury. However, the mechanism, by which polyamines mediate cold tolerance, is still to be explored. So in this paper, the conjugated polyamines in tonoplast and vacuole conformation were illuminated in the flesh cells of peach fruit subjected to cold stress, which were pretreated with exogenous putrescine. The results showed that under cold condition, fruit firmness decreased and flesh browning area increased, and vacuole conformation changed. The cold injury could be alleviated by pretreatment with exogenous putrescine, as judged by stabilization of the vacuole conformation, increased fruit firmness and reduced flesh browning area. Furthermore, the cold alleviation was coupled with the increases in the levels of covalently conjugated putrescine and non-covalently conjugated spermidine and spermine in the tonoplast. The results suggested that these conjugated polyamines in tonoplast and vacuole conformation might be involved in putrescine-mediated cold tolerance. The suggestion was further verified by applications with inhibitors, phenanthroline and methylglyoxal-bis (guanylhydrazone) (MGBG). Phenanthroline and MGBG could restrain the putrescine-induced increases in covalently and non-covalently conjugated polyamines mentioned above in the tonoplast, respectively, and increase flesh browning area, decrease fruit firmness and vacuole conformation stabilization. So, we can conclude that exogenous putrescine alleviates cold injury in peach fruit via elevating the contents of the polyamines conjugated to the tonoplast and thereby maintaining vacuole conformation.

The aim of this study was to determine the effect of a granular poultry manure fertiliser on the yield and the quality of white cabbage (Brassica oleracea var. capitata), and to determine the effect on the soil agrochemical properties. The effect of the granular poultry manure fertiliser and mineral fertiliser (applied separately and combined at different times - in early spring and in autumn) on the cabbage yields was determined. Investigations were carried out in the fields of the Institute of Horticulture, the Lithuanian Research Centre for Agriculture and Forestry. The object of the research - white cabbage 'Socrates' H. The highest white cabbage marketable yield (80.5 t/ha) and the highest amount of vitamin C (7.80 mg per 100 g of products) was obtained by applying the granular poultry manure fertiliser in the autumn and the mineral fertiliser in the spring, where the granular poultry manure fertiliser applied in the early spring and the granular poultry manure fertiliser applied in the autumn with the mineral fertiliser applied in spring retained a higher content of humus (1.82-1.94%), organic carbon (1.06-1.12%) and total nitrogen (0.099-0.147%). The mineral nitrogen residue in the soil decreased when the cabbage was fertilised in the spring with the mineral fertiliser and when fertilised in the spring with the mineral fertiliser in combination with the granular poultry manure fertiliser by 8.1-10.8 kg/ha. The minimum content of nitrates in the heads of the white cabbage was found after using the granular poultry manure fertiliser in the autumn.

In an environment where fruit production is increasingly affected by unpredictable weather patterns, it is important to look for ways to minimise the impact of climate change on production. Under Central European conditions, a limiting factor for apricot (P. armeniaca L.) and Asian plum (P. salicina Lindley) growing in certain years is the occurrence of late spring frosts. One measure to eliminate their impact is to delay the actual flowering of the trees. This can be done by breeding or just by applying various plant growth regulators (PGRs). In our experiment, the effect of a total of 6 active substances in 16 different concentrations was evaluated. The results show that the application of Ethrel-based mixtures (concentrations of 0.05 and 0.5%) had the greatest influence, delaying flowering by up to 3-5 days, but also had the most destructive effect on tree health. The application of the commercial product Rhodofix (NAA - 0.3%) and the application of a proprietary mixture based on NAA 1.0% did not have a very significant effect, with a delay in flowering of just 2 to 3 days. One interesting finding was that the application of the above products had a statistically significant effect on the ripening date of apricot fruit, with a difference of up to 4 days.