Raspberry has acquired great interest in human health due to its content of bioactive compounds that provide protection against diseases caused by non-communicable diseases. Bioactive compounds are mainly represented by secondary metabolites such as phenols, anthocyanins, and flavonoids. Biostimulants and elicitors are substances or microorganisms that provide protection and defence to the physiological processes of plants. The present study evaluated the effect of two elicitors (hydrogen peroxide, salicylic acid) and three biostimulants (humic and fulvic acids, glutamic acid, seaweed extracts) on the content of bioactive compounds in raspberry fruits, agronomic and fruit yield parameters in plants. Hydrogen peroxide increased the content of bioactive compounds such as flavonoids, anthocyanins, omega 3 and oleic acid. Salicylic acid increased the content of flavonoids, anthocyanins, and citric acid in raspberry fruits; the number of fruit loaders and fruits per plant was also increased. Humic and fulvic acids, glutamic acid, and glutamic acid combined with seaweed extracts increased the content of flavonoids and anthocyanins, without affecting growth parameters and fruit yield. Glutamic acid and seaweed extracts were the only treatments that increased the content of palmitic acid, while seaweed extracts increased °Brix content in fruits.

The excessive use of chemicals in plant production, including mineral fertilizers has a harmful effect on the morpho- and physiological state of strawberry plants, their yielding, and the physicochemical properties of the fruit and soil. Increasing the effectiveness of beneficial microorganisms, i.e. filamentous fungi, arbuscular mycorrhizal fungi (AMF) and bacteria, is an essential method of reducing the amount of fertilizers used to fertilize the soil. The aim of the study was to assess the effect of beneficial soil microorganisms (filamentous fungi and bacteria) applied alone or together with mineral fertilizers on the morpho- and physiological state of ‘Marmolada’ strawberry plants, their yielding, and the physico-chemical properties of the fruit. The experiment included the application of fungi (Aspergillus niger and Purpureocillium lilacinum), bacteria (Bacillus sp., Bacillus amyloliquefaciens and Paenibacillus polymyxa) alone or together with mineral fertilizers (Polifoska 6, Urea, Super Fos Dar 40). The study investigated the number of inflorescences and flowers, the intensity of the green colour of leaves, fruit yield and weight (g and %), pH, extract (°Brix), titratable acidity (g/100 g), fruit firmness (N), as well as the concentrations of macro-and microelements in the leaves of the plants. The obtained results depend on the type of fertilizer and its concentration. Strains of filamentous fungi or bacterial strains only (without fertilizer), increase the fruit yield by 60 and 35%, respectively. Reduced to 60% doses of Polifoska 6, Urea, and Super Fos Dar 40 combined with beneficial soil microorganisms influence the strawberry fruit yield more beneficially than the fertilizers applied in standard doses (100%). The mineral fertilizers enriched with soil beneficial fungi and bacteria increase the mean fruit weight by 25–30%. Both Super Fos Dar 40 enriched with bacterial strains and Polifoska 6 at 100% positively affect the physical and chemical properties of strawberry fruits. The application of reduced to 60% doses of Fos Dar 40 and Polifoska 6 enriched with beneficial bacterial strains increase the nitrogen and potassium contents in the leaves, respectively. Urea and Super Fos Dar 40 at 100% enriched with beneficial bacteria positively affect magnesium content in the leaves. Application of Polifoska 6 and NPK enriched with bacteria beneficially influences the iron content in the leaves. The calcium content in strawberry leaves decreases due to fertilization.

Rosa damascena is one of the oldest valuable rose flower varieties that is almost drough tolerant. However, selecting and identifying landraces that are more tolerant to drought conditions will be effective in developing the cultivation of the plant under stress. The most important step in developing drought-tolerant plants is the evaluation and identification of resistant and susceptible genotypes. In this case, an experiment was performed under in vitro conditions using five levels (0, 25, 50, 75, and 100 g/L) of polyethylene glycol (PEG) on four landraces of the Damask rose (Maragheh, Urmia, Pakdasht, and Kashan). Based on the findings, the resistance of the different landraces to a water deficit was measured by the Design-Expert software using the response level (RSM) method. The results showed that Maragheh, with the highest fresh and dry weight, total chlorophyll, chlorophyll a, chlorophyll b, proline and an increase in the superoxide dismutase activity, had a high tolerance to drought stress. Moreover, Maragheh with a decreasing leaf number, height, malondialdehyde, hydrogen peroxide and having a higher membrane stability index, showed a better defence mechanism against oxidative stress than the other landraces. Also, after Maragheh, Pakdasht had the best performance compared to the other two landraces up to 75g/L of PEG, but not as well as Maragheh at the highest level of the tested PEG. Urmia and especially Kashan probably do not have much tolerance to drought stress regarding all the results and levels of desirability.

Apples (Malus domestica) are one of the most important temperate fruit crops in the world. Apple production provides, directly or indirectly, employment and improvement in the living standards to millions of people, playing a key role in rural economies. We have conducted an interview-based survey to assess the current status and challenges faced by apple production in the Batna and Khenchela provinces, the two most important apple producing regions of Eastern Algeria. The survey was conducted between March and November 2019. A total of 50 apple growers answered 40 questions, including 13 direct and 20 qualitative questions. A scale ranging from 1 (very low) to 5 (very high) was used as a quantitative measurement for 12 questions. The reliability index of the questionnaire was 0.827. The results identified the main challenges faced by apple growers in Eastern Algeria as: limited access to certified and clean planting material, limited knowledge of disease and pest identification, inadequate access to appropriate disease management, lack of storage facilities and transportation of harvested fruit, and insufficient knowledge of marketing and access to markets. The study also identified a need for the development of cost-effective plant multiplication, horticultural and disease management practices and educational programmes suitable for local conditions to enhance the productivity and economic benefits of apple orchards.

This paper presents the important features of indigenous wild cherry genotypes suitable for processing. The research took place between 2017–2020, having ten Romanian wild cherry genotypes as study material. The harvesting maturity was recorded in the first decade of June for the early cultivar (G10) and decades two and three of June for the other studied genotypes, all of them with a middle season maturation. The number of days between the end of the flowering and the maturation was between 54 days (G10) and 66 days (G9). The average weight of the fruit and the equatorial diameter varied between 2.1 g and 13.80 mm for G4, respectively, and 5.5 g and 21.35 mm for G10. The proportion (%) between the pulp and waste (stone and peduncle) was measured as 81.95% pulp (G4) and 93.64% pulp (G10). The values of the soluble dry substance content were between 13.1–24.0 °Brix, the titratable acidity was between 0.48–1.16 mg malic acid/100 mL juice and the total content of the polyphenols was between 229.00–720.00 mg GAE/100 mL fresh juice. In terms of the bitter taste intensity, G10 has a weak intensity, G3, G7, G8 and G9 have an average intensity and G1, G3, G4, G5 and G6 have a high intensity.

Phenolic compounds limit micropropagation of many orchids in vitro. The aim of the study was to estimate the effect of activated charcoal (AC);1, 2 or 4 g/L) or ascorbic acid (AA; 10, 20 or 30 mg/L) added to the half strength MS medium on the growth and o-dihydroxyphenols content in Paphiopedilum insigne in vitro. A positive effect of AC on the shoot and root formation has been found. The highest multiplication rate (5.6 shoots/explant) and rooting frequency were obtained on medium containing 2 g/L of AC. However, AC reduced the leaf number as compared to the control. The lowest content of o-dihydroxyphenols was marked in Paphiopedilum insigne leaves when the shoots were grown on medium with 10 mg/L AA, followed by AC at 1 or 2 g/L.

Potassium (K+) deficiency inhibits the transport of photosynthetic products and causes severe crop yield losses. However, the underlying mechanisms are poorly understood. In this study, we used two tomato lines 081018 (K+-deficiency-sensitive) and 081034 (K+-deficiency-tolerant), showing tolerance to K+ deficiency to investigate the relationship between the H2O2 and sucrose in the tomato under K+-deficiency. The H<sub>2</sub>O<sub>2</sub> accumulation was increased by the low K+ condition (0.5 mM) after 8 h in 081018. The enzymes related to the metabolism of H<sub>2</sub>O<sub>2</sub> were decreased, and more malondialdehyde (MDA) was produced. After 24 h, the sucrose content had accumulated significantly in the leaves, however, it was deficient in the roots, and the expression level of the sucrose transporters (SUT1) was inhibited. In 081034, the activity of antioxidant enzymes was increased under K+-deficiency, and then the H2O2 subsequently returned to the control treatment (4 mM) levels and did not produce more MDA. The sucrose content was not significantly different from the control treatment after 24 h. The expression of SUT1 was not suppressed. These results suggested that the H2O2 dynamics played different roles in the two different strains. The transportation of sucrose was suppressed by the H<sub>2</sub>O<sub>2</sub> from the leaf (source) to the root (sink) in 081018, and unrestricted by the advantageous reactive oxygen species dynamics capacity in 081034.

Plenty of studies have demonstrated that DIF has an effect on the fruit growth. To study the effects of day and night temperature differences on the strawberry sugar quality, an experiment using climate chambers was conducted. Five different differences between the day and night temperatures (DIF) were set, which were 6 °C (28 °C/22 °C, day/night temperature), 8 °C (29 °C/21 °C), 10 °C (30 °C/20 °C), 12 °C (31 °C/19 °C), 14 °C (32 °C/18 °C). The results showed the following indices peaked with a DIF of 12 °C, including the photosynthesis rate, glucose content, fructose content, sucrose content, soluble sugar content and sugar metabolic enzyme activity. The respiration rate increased with the DIF during the day and decreased with the DIF at night. The root dry weight peaked at a DIF of 10 °C, the stolon dry weight peaked at a DIF of 8 °C and the leaf dry weight peaked at a DIF of 6 °C; however, the fruit dry weight reached maximum values at a DIF of 12 °C. The Grey correlation analysis showed that the most important factor in our experiment affecting the fructose content was the sucrose phosphate synthase; however, for the sucrose, glucose, and soluble sugars, the most important factor was the photosynthesis. We found that a DIF of 12 °C (31 °C/19 °C, day/night temperature) was the most suitable for strawberry growth, especially for the sugar content accumulation.

Mediterranean farming is facing increasing periods of water shortage and, in the coming decades, the water reduction is expected to exert the most adverse impact upon growth and productivity. This study was performed to assess the response of the physico-biochemical quality parameters of mango fruits to different doses of irrigation in a Mediterranean subtropical area in Spain. During two-monitoring seasons, trees were subjected to deficit-irrigation strategies receiving 33, 50, and 75% of a crop evapotranspiration (ETC), and a control at 100% ETC. According to the findings and respect to control, the yield was reduced in 8, 11, and 20% for the water-stressed trees at 75, 50, and 33% ETC, respectively, producing smaller fruits in line with the amount of applied irrigation. However, the water-stressed fruits significantly enhanced their quality, in particular at 33% ETC, with regards to the content of the health-promoting phytochemicals (total soluble solids, vitamin C, and β-carotenoids). Thus, sustainable water management without a detrimental effect on the yield could be possible, and farmers should be encouraged to adapt to the environmental constraints, producing improved quality fruits.

The flowering and quality of Freesia refracta Klatt ‘Argentea’ were assessed after the application of fungi of the Trichoderma genus and assimilation lighting. The assimilation lighting accelerated the flowering of the Freesia refracta ‘Argentea’ plants by 3–4 weeks. The fungi of the Trichoderma genus accelerated the flowering of the Freesia refracta ‘Argentea’ plants with light deficit by about one week. The assimilation lighting resulted in the development of shorter main inflorescence shoots regardless of the fact whether the plants had been treated with the fungi of the Trichoderma genus or not. The assimilation lighting and the fungi of the Trichoderma genus stimulated the development of lateral inflorescence shoots in the ‘Argentea’ cultivar. The fungi of the Trichoderma genus stimulated the development of flowers in the ‘Argentea’ cultivar. This effect was particularly noticeable when the plants were exposed to the assimilation lighting. The assimilation lighting stimulated the uptake of potassium in the ‘Argentea’ cultivar. The fungi of the Trichoderma genus stimulated the uptake of phosphorus and calcium in the plants underexposed to light. They also stimulated the uptake of potassium in the plants exposed to the assimilation lighting. The assimilation lighting stimulated the uptake of microelements. The fungi of the Trichoderma genus stimulated the uptake of iron, manganese and zinc both in the plants exposed to the assimilation lighting and those underexposed to light. The assimilation lighting combined with the treatment with the fungi of the Trichoderma genus stimulated the uptake of copper.