Testicular torsion and detorsion are significant clinical issues for infertile men. Torsion of the spermatic cord is an emergency condition resulting from the rotation of the testis and epididymis around the axis of the spermatic cord. A rat testis model was used to assess the effects of polyethylene glycol on ischemia-reperfusion injury. Twenty-four healthy male Wistar rats were used. The rats were included and randomized into four investigational groups (n = 6): Group Sham: Merely laparotomy was implemented. Group Ischemia: Merely 3-hour interval ischemia was done. Group IS/REP: A 3-hour interval ischemia, 3-hour reperfusion for left testis, one-week reperfusion for right testis were done and 20 µl normal saline was administered intraperitoneally (IP) 30 min before termination of ischemia. Group IS/REP/PEG: The same as group IS/REP as well as 20 µl PEG solution 3% (IP) 30 min before termination of ischemia. Evaluations were based on biochemical analyses and sperm parameters morphometry. Polyethylene glycol enhanced antioxidant activity and quality of sperm parameters (p < 0.05). In conclusion, polyethylene glycol could be helpful in minimizing ischemia-reperfusion injury in testicular tissue exposed to ischemia

Case Description- A four-month-old female Holstein calf was presented to the Veterinary Teaching Hospital of Urmia University with a history of abnormal hairy growth on the right eye since birth. Clinical Findings- On general examination, the animal was alert. Body temperature, heart rate, respiratory rate, menace test, and pupillary light reflex were normal. On ophthalmic examination, a hairy mass was observed on the conjunctivae, third eyelid, sclera, and cornea of the right eye. Histopathological examination revealed the mass with three distinct areas of skin. It was diagnosed as an ocular dermoid cyst and surgical treatment was warranted. Treatment and Outcome- The mass was surgically excised to prevent damage to the anterior chamber of the eye. Ocular healing was satisfactory in a 4 week follow up. Clinical Relevance- This report described an ocular dermoid cyst in a four-month Holstein calf. There was just lacrimation, possibly due to irritation caused by the hairs. The ocular dermoid cyst could be excised surgically, and the prognosis is good.

Objective- The aim of the present study was to assess the wound-healing activity of extract of Allium stipitatum. Design- Experimental study.Animals- Thirty-six male Wistar diabetic rats.Procedures- The rats weighing approximately 160-180 g and seven weeks of age were randomized into three groups of 12 rats each: Control surgery group (Control) including the creation of wounds and no treatment, base formulation groups positive (POS) with the creation of wounds and application of base formulation ointment, treatment group 1 (T1) with 2 g of powder extract of the plant material in the ointment. A wound was induced by an excisional based wound model in male rats. The mature green leaves of Allium stipitatum were collected and authenticated. Extractions of dried leaves were carried out. For wound-healing activity, the extracts were applied topically in the form of ointment and compared to control groups. The healing of the wound was assessed based on the wound area, histomorphometry, and hydroxyproline estimation studies. Results- Reduction in the wound area and hydroxyproline contents indicated that there was a significant difference (p = 0.001) between group T1 and other groups. Quantitative histological studies and mean rank of the qualitative studies demonstrated that there was a significant difference between group T1 and other groups (p = 0.001).Conclusions and Clinical Relevance- The extract of Allium stipitatum leaves enhanced wound-healing activity significantly in both the wound models studied. Enhanced wound contraction, decreased epithelialization time, increased hydroxyproline content, improved histological characteristics studies suggested Allium stipitatum leaves extract might have therapeutic benefits in wound healing.

Objective- Cutaneous wound healing is an essential physiological process consisting of the collaboration of many cell strains and their products. Initiation of new management for treatment of wound infections caused by multidrug resistant Staphylococcus aureus is required. The aim of the present study was to assess wound healing activity of Ferula assa-foetida loaded chitosan nanoparticle biofilm in methicillin resistant S. aureus (MRSA) infected wounds in rats. Design- Experimental study Animals- Forty eight male healthy Wistar rats. Procedures- The animals were randomized into four groups of 12 animals each. In group I, the wounds were infected with MRSA and only treated with 0.1 mL the sterile saline 0.9% solution. In group II, the infected wounds were dressed with chitosan nanoparticles biofilm. In group III, animals with infected wounds were treated with 0.1 mL topical application of Ferula assa-foetida. In group IV, animals with infected wounds were dressed with Ferula assa-foetida loaded chitosan nanoparticles biofilm. Results- Microbiological examination, planimetric, biomechanical, histological and quantitative morphometric studies and determination of hydroxyproline levels showed that there was significant difference between animals in group IV compared to other groups (p = 0.001). Conclusion and Clinical Relevance- Ferula assa-foetida loaded chitosan nanoparticles biofilm could be useful for treatment of MRSA infected wounds in diabetes.

Objective- This study was designed to investigate the effect of Lactobacillus plantarum gel on cutaneous burn wound healing in diabetic rats.Design- Randomized experimental studyAnimals- Forty adult male ratsProcedures- Two circular 1 cm cutaneous wounds were created in the dorsum back of each rat. 48 h post-burning, debridement with a 1 cm biopsy punch was performed. The wounds were divided into the following four treatment groups (n= 10, each): 1. Untreated or negative control (NC), 2. silver sulfadiazine (positive control-SSD), 3. base gel (BG) 4. Lactobacillus plantarum Gel (LP gel). The wound surface area and epithelialization were monitored. The animals were euthanized at 10 (n = 5), and 20 (n = 5) days post-injury (DPI) and the skin samples were used for histopathological, biochemical, TGF-β gene expression and biomechanical investigations. Results- It was indicated that the L. plantarum and SSD treated lesions had the lowest percentage of wound size and collagen content and also the L. plantarum treated group showed shortest inflammatory period and highest amount of TGF-β at 10 days post injury. The L. plantarum gel treated lesions also demonstrated greater ultimate load compared to the untreated and based gel treated wounds.Conclusions and Clinical Relevance- In conclusion, L. plantarum gel therapy improved wound healing and resulted in better outcomes after severe burn injury in diabetic rats compared with the silver sulfadiazine treatment.

Objective: Busulfan(Bus) is a chemotherapy drug that is widely used for cancer treatment. The protective effect of CoQ10 evaluated on testis and sperm parameters after busulfan treatment. Design: Experimental StudyAnimals: Thirty tow adult male Wistar rats Procedures: In this experimental study 32 adult male Wistar rats have randomly divided into four groups: Control group received normal saline (0.1 mL, daily, intraperitoneally). Sham group received a single dose of busulfan 10 mg/kg, IP. Positive control group received 0.1 mL CoQ10 (10 mg/kg, IP). The treatment group received busulfan along with CoQ10 (10 mg/kg, IP). All procedures were continued for 35 days. For histomorphometric analyses, the thickness of testicular capsule, the germinal epithelium height and the diameter of the seminiferous tubules were measured. Semen analysis was used for the assessment of sperm parameters. Results: Histomorphometric analyses showed the thickness of testicular capsule was increased in busulfan groups (P<0.05). Compared to the control group, germinal epithelium height was decreased significantly in Sham and treatment groups (P<0.05). There was a significant decrease in seminiferous tubules diameter in busulfan and treatment groups compared to the control group. In Sham group, the sperm count, motility, viability, nuclear maturation and sperm morphology were significantly decreased compared to control and CoQ10 treated groups (P<0.05). The percentage of sperm with DNA damage in the CoQ10 treated group was significantly increased compared to Sham groups (P<0.05).Conclusion and Clinical Relevance: Administration of CoQ10 in busulfan-treated animals improved histological and sperm quality.

<strong>Case Description</strong>-An adult golden eagle with a wound on its right wing caused by gunshot was presented to the University of Tabriz, Veterinary Teaching Hospital.<br /> <strong>Clinical Finding</strong>-Clinical examination and radiographs, revealed compound fracture in distal third of humerus, severe soft tissue compromise and irreparable loss of blood supply. Anemia and leukocytosis were revealed on CBC test.<br /> <strong>Treatment and Outcome</strong>- Based on clinical and radiographic figures, amputation of the wing was considered to save the bird life. The bird was treated with Ringer's lactate solution and Lincospectin, preoperatively. Under general anesthesia with intramascular injection of Ketamine 10mg/kg and Xylazine 1mg/kg amputation of wing at proximal third of the humerus was done. The bird recovered without any complication. Healing was uneventful and no complications were observed during six months postoperatively.<br /> <strong>Clinical Relevance</strong>-Hereby, we reported unilateral wing amputation for management of humeral fracture due to gunshot in a Golden eagle. Amputation of the humerus at its proximal third was recommended to prevent osteomyelitis and as a life-saving treatment in such a complicated case.<br />

Objective- One of the important causes of nasal fracture is a trauma that can be diagnosed in clinical examinations and diagnostic imaging but it should be noted that radiography may show false fractures in the nose. This study evaluates the value of ultrasonography in the diagnosis of nasal fractures and compared it with plain radiography. Design- Retrospective study. Animals- 12 dog patients with suspected nasal fracture. Procedure- These dogs were evaluated by ultrasonography and radiography in comparison with the clinical examination (golden standard) and the results were analyzed by statistical method. Sensitivity, specificity, positive and negative predictive values, positive and negative odds ratio, and accuracy with 95% confidence intervals and the accuracy of both tests were calculated. Results- In the diagnosis of nasal fracture by ultrasonography, sensitivity was 96.4%, specificity of 93.3% and accuracy of 96% and in the radiographic method, sensitivity was 81.1%, specificity of 86.7% and accuracy of 82%. In ultrasonography and radiography, the most sensitivity in diagnosis of nasal bridge fracture was 100% and 92.6% and the most specificity in diagnosis of the outer wall nasal fracture was 100% and 50%, respectively. Conclusion and Clinical Relevance- According to the results of this study, ultrasonography can be used as a non-invasive method with high sensitivity and specificity in the diagnosis of nasal bone fractures in dogs.