Recent progress in tissue-engineered skin grafts has alleviated the demand for autologous split thickness skin grafts for treatment of large skin wounds. In this study, a series of cost-effective nanofibrous scaffolds aimed at full-thickness wound healing are fabricated by blending gelatin (Gel) with poly(l-lactic acid)-b-poly( ε-caprolactone) (PLLCL) and electrospun to obtain composite Gel/PLLCL nanofibers in four different weight ratios (w/w) of 80:20 [Gel/PLLCL(20)], 70:30 [Gel/PLLCL(30)], 60:40 [Gel/PLLCL(40)], and 50:50 [Gel/PLLCL(50)]. The mechanical properties of these nanofibrous scaffolds were evaluated in both dry and wet conditions, and the Gel/PLLCL(40) retained suitable tensile stress (1.16 ± 0.03 MPa) to be handled even in wet conditions. Moreover, the proliferations of fibroblast cells on Gel/PLLCL(40) were 15%, 7% and 10% higher compared to cell proliferations on Gel/PLLCL(20), Gel/PLLCL(30), and Gel/PLLCL(50), respectively. In vitro results confirmed Gel/PLLCL(40) as the optimized scaffold composition suitable for skin tissue engineering. The healing ability of this scaffold was studied in vivo using mouse models. The Gel/PLLCL(40) greatly accelerated wound closure and regeneration occurring in the first 10 days of implantation compared to the control group. In addition, newly regenerated epidermis was only found in the nanofibrous scaffolds–treated group, and it was comparable to the epidermis of normal skin.

Poly(ε-caprolactone) is an established polymer used in the fabrication of scaffolds for tissue engineering applications. Poly(ε-caprolactone)’s intrinsic hydrophobicity and toxicity, however, is greater than other natural polymers which limits its applicability. In this study, these problems were addressed by the modification of poly(ε-caprolactone) nanofibers with nanoparticles made from natural polymers, such as silk fibroin. Silk fibroin nanoparticles were prepared by desolvation and blended with poly(ε-caprolactone) to form a colloidal solution capable of forming nanofibers by electrospinning. Fabricated silk fibroin nanoparticles and three different nanofibers were characterized by transmission electron microscopy, variable pressure field emission scanning electron microscope, contact angle, Fourier transform infrared spectroscopy, thermogravimetric analysis, as well as an evaluation of their mechanical properties. The hybrid nanofibers incorporated with silk nanoparticles improved water absorbability compared to pure poly(ε-caprolactone) nanofibers and had much better mechanical properties than the silk fibroin nanofibers. The cytotoxicity and cell attachment tests were carried by culturing NIH 3T3 fibroblasts with the nanofibers. The hybrid nanofibers exhibited better cell viability and cell attachment than the pure poly(ε-caprolactone) nanofibers. Furthermore, the silk fibroin nanoparticles improved the water contact angle and enhanced cell interaction compared to the unmodified poly(ε-caprolactone). Based on these results, the modification of poly(ε-caprolactone) nanofibers with silk nanoparticles is a promising strategy for the improvement of poly(ε-caprolactone)-based nanofibers for future tissue engineering applications.

Crack prevention of biodegradable polymer coatings on drug-eluting stents was investigated by introducing a nano-coupled layer at the interface between the metal surface and the polymer coating layer using surface-initiated ring-opening polymerization of ε-caprolactone. Poly(d,l-lactide-co-glycolide) coating on cobalt-chromium control and ricinoleic acid-poly(caprolactone)–grafted cobalt-chromium was carried out using electrospraying. The cracking of the biodegradable polymer coating on drug-eluting stents during ballooning was addressed by introducing a nano-coupled interlayer on the cobalt-chromium surface. The ricinoleic acid-poly(caprolactone) nano-coupled interlayer and poly(d,l-lactide-co-glycolide)-coated top layer were characterized using attenuated total reflection Fourier transform infrared, contact angle, ellipsometry, X-ray photoelectron spectroscopy, and atomic force microscopy. Based on scratch tests, the nano-coupled samples had stronger interfacial adhesion compared to the control sample without the nano-coupled layer. Scanning electron microscope images indicated that the cracking on the poly(d,l-lactide-co-glycolide) coating was addressed. Introducing a nano-coupling interlayer may be an important strategy to preventing polymer coating cracking on drug-eluting stents.

This corrigendum is in reference to the manuscript “ Enzymatically cross-linked injectable gelatin gel as osteoblast delivery vehicle” Journal of Bioactive and Compatible Polymers July 2012 27: 342-355, DOI: 10.1177/0883911512444713 , by Ashley A Amini and Lakshmi S Nair.

The encapsulation of living cells within a semi-permeable matrix is an attractive process for transplanting nonautologous cells by limiting the interaction with the host immune system. The electrohydrodynamic process is a low-cost and high-throughput system to encapsulate cells by means of a static potential. We evaluated the use of this system for cell entrapment by assessing and then manufacturing capsules that had the best dimensions. The effect of different cell densities on the beads was determined to set up the basic parameters of the encapsulation system. The cell viability inside the beads and as a function of release time was observed for their biological response.

In recent years, progress in the field of hybrid materials has been accelerated through use of the sol–gel process for creating materials and devices, which benefit from the incorporation of both inorganic and organic components. In this work, organic–inorganic hybrid membranes were prepared from tetraethoxysilane and a blend system composed of chitosan and soy protein. By introducing a small amount of siloxane bond into the chitosan/soy protein system, the chitosan/soy protein hybrid membranes were improved in terms of structure, topography and mechanical properties. It appears that the chitosan/soy protein hybrid membranes were formed by discrete inorganic moieties entrapped in the chitosan/soy protein blend, which improved the stability and mechanical performance assessed by the dynamic mechanical analysis as compared to chitosan/soy protein membrane. Also, in vitro cell culture studies evidenced that the chitosan/soy protein hybrid membranes are non-cytotoxic over a mouse fibroblast-like cell line. The hybrid membranes of silane-treated chitosan/soy protein developed in this work have potential in biomedical applications, including tissue engineering.

This study describes a strategy for the covalent immobilization of active adhesion peptide moieties onto polymers through the intermediacy of itaconic acid. The arginine–glycine–aspartic acid peptide was grafted to a novel poly(caprolactone 2-(methacryloyloxy) ethyl ester)- co-itaconic acid bulk biomaterial, in order to improve the cell adhesion of the polymer. First, the arginine–glycine–aspartic acid sequence was grafted onto itaconic acid via an amidation reaction using N-(3-dimethylaminopropyl)- N′-ethylcarbodiimide hydrochloride/ N-hydroxysuccinimide as activation complex. The itaconic acid–arginine–glycine–aspartic acid macromer was characterized by Fourier transform infrared spectroscopy and 1H-NMR, yielding a functionalization degree of 85%. In a second step, poly(caprolactone 2-(methacryloyloxy) ethyl ester- co-itaconic acid–arginine–glycine–aspartic acid) (with a feed mixture of 90 wt% of caprolactone 2-(methacryloyloxy) ethyl ester and 10 wt% of itaconic acid–arginine–glycine–aspartic acid macromer) and a series of copolymers of caprolactone 2-(methacryloyloxy) ethyl ester and itaconic acid with different compositions (weight fractions of itaconic acid up to 20 wt%) were synthesized by radical copolymerization. The microstructure and network architecture of the new polymer systems were investigated. Mechanical moduli of poly(caprolactone 2-(methacryloyloxy) ethyl ester- co-itaconic acid), evaluated by dynamic–mechanical analysis, increase with the itaconic acid content. In poly(caprolactone 2-(methacryloyloxy) ethyl ester- co-itaconic acid–arginine–glycine–aspartic acid), the glass transition temperature and the mechanical moduli of the system are smaller than in the nonfunctionalized poly(caprolactone 2-(methacryloyloxy) ethyl ester- co-itaconic acid) copolymers, and the polymer is less hydrophilic. The results indicate that arginine–glycine–aspartic acid grafting of poly(caprolactone 2-(methacryloyloxy) ethyl ester- co-itaconic acid) copolymer networks can be useful for tissue engineering applications, because regenerative processes in the nervous system can be promoted and accelerated, thus, opening a possibility to generate materials with a high potential for clinical applicability.

To address the problem of surface-induced thrombosis and restenosis, an extracellular matrix–like biological membrane was constructed from collagen, heparin, vascular endothelial growth factor, and an anti-CD34 antibody. This membrane was assembled on a titanium surface using a layer-by-layer self-assembly technique and induced the spontaneous endothelialization of synthetic cardiovascular implants in vivo. The multilayer growth process was carried out by first depositing a single layer of positively charged poly-L-lysine on the negatively charged NaOH-treated titanium substrate. This was followed by alternating depositions of negatively charged heparin, containing vascular endothelial growth factor and an anti-CD34 antibody and positively charged collagen, terminating with an outermost layer of heparin containing vascular endothelial growth factor and the anti-CD34 antibody. The uncoated and coated titanium samples were exposed to platelet-rich plasma and endothelial progenitor cells, respectively, under static and flow conditions in vitro. Then, the samples were implanted into dog femoral arteries. The results suggest that the multilayering process led to reduced platelet adhesion and activation, promoted the attachment and growth of endothelial progenitor cells in vitro, and induced the rapid and complete endothelialization of the lumenal surface of the implant. Thus, the approach described here may be used in the fabrication of titanium-based vascular implant surfaces to induce endothelialization in vivo.

The rapid protein adsorption on a material surface causes blood coagulation, platelet activation, and complement system activation, which poses a risk for failure of cardiovascular devices. In this study, a chemically hydroxylated titanium surface was aminosilanized and covalently grafted with poly(ethylene glycol). The reaction conditions on the grafted quantity were studied by the respective amine and carboxyl densities. The blood compatibility of the PEGylated surfaces with different poly(ethylene glycol) densities and chain lengths was evaluated; the PEGylated surfaces with higher grafted density and longer chain length had less fibrinogen adsorption, less fibrinogen γ-chain exposed, less adherent platelets, and lower activation of the adherent platelets. In addition to the influence on blood, the longer chain PEGylated surfaces resisted, not only smooth muscle cell attachment and proliferation, but also macrophage attachment and death. This method is a good candidate for improving cardiovascular implant surfaces.

Drug eluting coronary stents coated with a bioactive and biostable polymer system (THBA70), based on acrylate copolymers bearing a salicylic acid derivative as side substituent, were evaluated. The microstructural architecture of the copolymer THBA70 comprises a random copolymer of a methacrylate derivative from triflusal (4-trifluoromethyl salicylic acid) with 45 mol% of THEMA (2-methacryloyloxyethyl [2-(acetyloxy)-4-(trifluoromethyl)] benzoate), which makes the system anti-thrombogenic with good adhesion to the surface of metallic stents. The bioactive coating prevented thrombosis, an adverse effect associated with the implantation of drug-eluting stents. The in vitro drug delivery of drug-eluting stents under dynamic conditions indicated excellent controlled drug release. The THBA70 films loaded with relatively low concentrations of taxol or simvastatin in contact with fibroblasts produced inhibition of cell proliferation with a dose-dependent bioactivity.