Autologous platelet rich plasma (PRP) plays an important role in soft tissue healing. The purpose of this study was to evaluate the efficacy of this facial skin treatment through objective parameters. Biometric parameters, including anisotropy, transepidermal water loss (TEWL), micro-relief, and hydration were used to determine the relative success of the treatment. The study, which included 37 female patients, demonstrated an average of 24% improvement in the anisotropy coefficient after the third week. The TEWL and micro-relief parameters also improved significantly (P < 0.05 in all parameters tested). In addition to these results, this study suggests that certain measured values may be used to predict the success of the aesthetic use of PRP.