Outside Front Cover: The cover image is based on the article Optimised Ribosome Profiling Reveals New Insights Into Translational Regulation in Synchronised Chlamydomonas reinhardtii Cultures by Yen-Ling Lin et al., https://doi.org/10.1111/pce.15681.

ABSTRACTSoybeans, one of the most important legumes, face multiple stresses including drought and herbivory. But the potential transfer of the interactive effects of these stressors onto the transgenerational plants has not been understood. We hypothesised that progeny from stressed parents will have enhanced ecophysiology and defence traits through priming, affecting fitness. Experimental plants were derived from seeds of drought (D), drought and herbivory interaction (D × H), herbivory (H) and well‐watered (WW) treatments imposed on parental generation. The findings revealed significant transgenerational effects of D, D × H, H and WW treatments; D × H treatment had negative impacts on seed germination and seedling vigour. The percent change in leaf and root nitrogen and protein content in seedling roots and leaves in D × H compared to D revealed possible remobilisation of nutrients in transgenerational seedlings. The transgenerational effects due to D × H persisted throughout the growing period reducing physiological parameters including net photosynthesis rate and stomatal conductance. But the trichomes were significantly induced indicating potential tradeoff for strengthened defences confirmed by soybean looper bioassays. And the tradeoff between defence and morpho‐physiology also led to negative effects on yield and fitness. Taken together, transgenerational induction, offers opportunities for developing resilient crops under multiple stressors like drought and herbivory.

ABSTRACTLegume cultivation has great potential for improving soil microbial communities and soil quality in reclaimed ecosystems. However, little is known about the effects of long‐term legume cultivation on the soil microbial community structure in agroecosystems in coal mine reclamation areas. To address this issue, we used metagenomics to examine the variations in the soil bacterial community composition and functionality under three leguminous crops in a 10‐year reclamation experiment. After 10 years, we found dissimilarity in the microbial communities between the control and leguminous crops. Furthermore, three legume cultivation greatly promoted the diversity and abundance of the dominant bacteria The keystone phyla of the soil bacterial communities in the reclamation area included Proteobacteria, Acidobacteriota, Gemmatimonadota and Myxococcota. In particular, soil pore structure was the main factor influencing the variation in the bacterial community. Therefore, long‐term legume cultivation can significantly improve the soil microbial community structure, which likely serves as the basis for soil microbial abundance and functionality and soil health. This study confirmed the ability of the spongy structure formed by legumes to optimize resource use in a nutrient‐impoverished mine reclamation agroecosystem. These findings may have important implications for assessing microbial multifunctionality in agroecosystems in coal mine reclamation areas.

Outside Front Cover: The cover image is based on the article Mitochondrial Energy Homeostasis and Membrane Interaction Regulate the Rapid Growth of Moso Bamboo by Yanli Gao et al., https://doi.org/10.1111/pce.15559.

The 18O enrichment (Δ18O) of cellulose (Δ18OCel) is recognized as a unique archive of past climate and plant function. However, there is still uncertainty regarding the proportion of oxygen in cellulose (pex) that exchanges post-photosynthetically with medium water of cellulose synthesis. Particularly, recent research with C3 grasses demonstrated that the Δ18O of leaf sucrose (Δ18OSuc, the parent substrate for cellulose synthesis) can be much higher than predicted from daytime Δ18O of leaf water (Δ18OLW), which could alter conclusions on photosynthetic versus post-photosynthetic effects on Δ18OCel via pex. Here, we assessed pex in leaves of perennial ryegrass (Lolium perenne) grown at different atmospheric relative humidity (RH) and CO2 levels, by determinations of Δ18OCel in leaves, Δ18OLGDZW (the Δ18O of water in the leaf growth-and-differentiation zone) and both Δ18OSuc and Δ18OLW (adjusted for εbio, the biosynthetic fractionation between water and carbohydrates) as alternative proxies for the substrate for cellulose synthesis. Δ18OLGDZW was always close to irrigation water, and pex was similar (0.53 ± 0.02 SE) across environments when determinations were based on Δ18OSuc. Conversely, pex was erroneously and variably underestimated (range 0.02-0.44) when based on Δ18OLW. The photosynthetic signal fraction in Δ18OCel is much more constant than hitherto assumed, encouraging leaf physiological reconstructions.