Abstract Oats ( Avena sativa L.) are cultivated worldwide for food and feed. Recently, there has been a growing interest in oats in South Korea owing to their nutritional and feed value. To support efficient breeding and genetic resource management, this study evaluated seven agronomic traits of 506 Avena accessions collected globally with two Korean oat varieties as standards. Seven agronomic traits – days to heading (DTH), plant height (PH), plant type, dehulling characteristics, 1,000-grain weight (TGW), seed length, and seed width (SW) – were evaluated over two to three years under Korean spring conditions. An augmented design was employed due to a lack of seeds and the field, and environmental bias was adjusted using Best Linear Unbiased Predictions. Statistical analyses revealed a strong positive correlation between TGW and SW and a moderate positive correlation between PH and DTH. Principal component analysis (PCA) identified that the first two principal components (PCs) explained 66.88% of the total variance, and that PC1 and PC2 were highly correlated with grain characteristics. K-prototype clustering analysis classified the 506 oat genetic resources into four distinct clusters based on the results of PCA, rather than their geographical origins. Cluster analysis indicated that Clusters 1 and 3 have potential for forage oat breeding, particularly in fodder use, whereas Clusters 2 and 4 may be valuable for grain-use oat breeding in South Korea. This study provides a comprehensive phenotypic assessment of oat germplasm obtained globally under Korean spring conditions and provides a useful foundation for germplasm management, cultivar development, and future genetic research.

Abstract The global ex situ conservation system reflects the collective efforts of individual genebanks, each influenced by its unique history, opportunities for acquisition and user demands. As a result, acquisition activities in most genebanks have been opportunistic rather than strategic to support representation of national crop diversity or driven by clearly identified conservation priorities. The objective of this study was to characterize the composition of national collections in 20 countries within the global ex situ conservation system. It assessed the representation of crop genetic diversity that originated nationally within the global system and evaluated the composition balance, as it relates to duplication and gaps in conservation. Across these 20 countries, numerous genebanks are conserving large number of accessions for only a few genera, while a much smaller number of genebanks are conserving fewer than 10 accessions from a broader range of genera. The findings indicate that ex situ conservation, both nationally and globally, has significant potential duplications and gaps in the genetic resource coverage. Individual institutes in the global system must carefully reconsider the composition of their collections, especially given the significant costs and limited financial and human resources available for long-term ex situ conservation. As national genebanks and the global system become increasingly important resources for the future, it will be essential for genebanks to ensure that the composition of their collections meets future demands.

Abstract Mulberry ( Morus spp.), a key moriculture crop, serves a dual role: its leaves are indispensable as exclusive fodder for silkworms, while its fruits, particularly in species such as Morus nigra, Morus laevigata and Morus macroura , are valued as functional foods enriched with antioxidants and vitamins. The genus exhibits remarkable natural chromosome-level variation ranging from 14 to 308, which offers a unique system to investigate the impact of genome expansion on plant function. Herein, the influence of different cytotypes on functional traits associated with cell division, cell size and biomass metrics was studied. Principal component analysis revealed that tetraploid (4x), followed by triploid (3x), exhibits the most favourable cytotype, suggesting a vigorous and balanced expression of polyploid advantage. However, downsizing of many key parameters with increasing ploidy levels was also evident, predominantly at 22-ploid (22x). Furthermore, the estimated plasticity index reveals that the lower limit of cell size and rate-limited attributes influenced the loss of growth superiority of the extreme ploidy level; in turn, data imply the large-genome constraint in mulberry, as the consequence of the functioning of a large genome is likely expensive. Therefore, the present study emphasizes that the benchmarking of ploidy levels for the selection of parental material is essential for improving cellular function and developmental efficiency, thereby optimizing leaf yield for the future sustainability of sericulture.

Abstract This study examines the critical situation faced by Sudan’s Agricultural Plant Genetic Resources Conservation and Research Centre (APGRC) during an ongoing civil war. The center houses over 17,000 accessions of diverse crop species, including globally significant collections of sorghum and pearl millet, which represent an irreplaceable repository of agricultural biodiversity. Recent militant attacks have severely damaged the center’s infrastructure and collections, threatening decades of conservation. Through an analysis of recent reports and institutional documentation, we document the APGRC’s history and achievements, assess current conflict impacts, and propose a framework for recovery and long-term resilience. The international response, including emergency seed rescue operations and safety duplication at the Svalbard Global Seed Vault, demonstrates the vital importance of global cooperation in preserving plant genetic resources during armed conflicts. This case highlights the vulnerability of ex-situ conservation facilities in politically unstable regions and the need for decentralized conservation networks, robust safety duplication systems, and sustained international support. We presented a phased recovery plan that addresses immediate needs, medium-term stabilization, and long-term resilience building. The global community has a shared interest in preserving the unique crop diversity of Sudan, particularly its drought-tolerant sorghum and millet varieties, which may be the key to agricultural adaptation to climate change. The response to the APGRC crisis demonstrates the recognition of this shared interest; however, sustained commitment is needed to ensure the long-term conservation of Sudan’s irreplaceable plant genetic heritage

Abstract Noug ( Guizotia abyssinica L.) is among the key oilseed crops of Ethiopia and India and is valued for its tolerance to drought, with its very nutrient-dense oil being rich in essential fatty acids, protein and minerals. Despite the economic potential of noug, its production is limited by biological factors such as self-incompatibility, seed shattering, parasitic weeds and disease susceptibility. This review consolidates advances in the past decade, highlighting the transition from conventional breeding to the application of genomic tools. We critically assess how the development of self-compatible lines, transcriptome sequencing and linkage mapping are being used to produce improved germplasm. There is, however, a huge gap between research products and farmer-preferred variety development. This review suggests an integrative approach that brings genomics, phenomics and participatory breeding to realize the faster generation of high-yielding, durable noug cultivars. This is an important strategy to ensure that noug is a sustainable crop that ensures food security and financial empowerment of Ethiopian smallholder farmers.

Abstract The conservation of plant genetic resources for food and agriculture (PGRFA) is essential for global food and nutrition security, climate resilience and sustainable agriculture. This study presents a monitoring and evaluation (M&E) framework applied across 20 countries in the Global South to assess the state of ex situ PGRFA conservation. Using mixed methods, we collected quantitative and qualitative data on genebank performance, validated operational status, and identified specific needs of genebanks. Our findings demonstrate the practical use of this M&E framework for genebanks to assess efficiency and effectiveness in genebank management, while also guiding targeted capacity development and context-specific interventions to address persistent challenges. We recommend adopting the approaches described in this study, including genebank reviews and the review checklist, to support more systematic and evidence-based assessment of genebanks. Aligning national efforts with global conservation objectives will help generate actionable recommendations to strengthen genebanks and ensure the long-term conservation and availability of PGRFA.

Abstract A set of 710 accessions of global durum wheat panel was obtained from the International Centre for Agricultural Research in Dryland Areas (ICARDA) and evaluated for variability in 32 agro-morphological traits at the ICAR-Indian Institute of Wheat and Barley Research, Karnal, India, during 2021–22 and 2022–23. The accessions were grouped based on the type of breeding material and geographical origin, and the Shannon–Wiener diversity index (H′) was calculated for each trait as well as each group. The H′ values ranged from 0.02 (grain crease) to 1.27 (wax of ear) with an overall mean of 0.63, when considering the entire set as a single group. Landraces exhibited higher variability (H′ = 0.64) as compared to released cultivars and evolutionary pre-breeding population lines (H′ = 0.55). Furthermore, accessions originating from West Asia exhibited the highest variability (H′ = 0.63). Traits such as coleoptile colour, plant growth habit, auricle colour and pubescence, flag leaf attitude, ear shape, presence or absence of awns, awns colour and attitude, glumes shape and pubescence, beak length, peduncle attitude, grain phenol colour, grain germ width and brush hair length were found suitable for distinguishing the present durum accessions due to their higher frequency distribution and Shannon–Wiener diversity index values. A core set of 13 accessions was developed using the programme Power Core. These accessions represent the overall variability of the entire set and can therefore serve as a potential source of new genes/alleles for the genetic enhancement of durum wheat. This durum panel may also serve as a valuable reference collection for global researchers in the characterization of durum wheat accessions.

Abstract Pteridium aquilinum, commonly known as bracken, is a fern species distributed in temperate and subtropical regions. In Iran, it primarily grows in isolated populations in the northern regions, and its genetic structure, gene flow and population genetics are not well understood. This study aimed to investigate gene flow and genetic diversity in 11 geographical populations of P. aquilinum in Iran for the first time. A total of 133 plants from different populations were examined, and both morphological and genetic diversity were assessed using start codon targeted (SCoT) molecular markers. Results showed genetic and morphological admixture within populations, likely due to the high mobility of dispersal units in this species. Significant genetic differences were found among populations, but a high degree of genetic variability was observed within individual populations. Additionally, there was a significant correlation between geographical and genetic distances, indicating gene flow between populations. The assignment test revealed genetic admixture among plants from different populations, and Gst analysis of SCoT loci yielded Nm = 0.95, confirming gene flow among the populations. Overall, this study demonstrates that the Iranian populations of P. aquilinum maintain substantial gene flow, which may contribute to the long-term survival and adaptability of this species in the region.

Abstract Distinctness, uniformity and stability (DUS) studies are essential components of assessment and registration processes for plant varieties, including those within the Morus alba species, commonly known as white mulberry. M. alba L. holds a unique place in agriculture sciences due to its historical significance in silk production and its cultivation for its edible fruits. This study provides an overview of DUS studies in M. alba carried out in the Department of Tree Improvement and Genetic Resources, Dr Yashwant Singh Parmar University of Horticulture and Forestry, Nauni, Solan (Himachal Pradesh) India, highlighting the critical features and characteristics evaluated to distinguish between different cultivars. It explores the factors contributing to uniformity and the stability of these characteristics over time, emphasizing the importance of these studies in guiding breeding programmes, supporting agricultural practices and preserving the diversity within this species. The study includes DUS characterization of M. alba germplasm based on 3 qualitative, 11 pseudo-qualitative and 11 quantitative characteristics. DUS studies play a pivotal role in ensuring the accuracy of cultivar identification, thus facilitating the sustainable cultivation and conservation of M. alba, a species with deep-rooted cultural and economic significance. It is concluded that many varieties with distinct and distinguishable characteristics and better economic and genetic values can be registered for their protection under the PPV&FR Act, 2001 and can be used in breeding programmes.

Abstract This study investigates the potential of Emblica officinalis Gaertn. (aonla) for cultivation across diverse soil types, with a particular focus on alkaline soils, as part of broader efforts to enhance biodiversity, promote sustainable agriculture and improve climate resilience. This experiment was focused on identifying and characterizing aonla accessions for alkaline conditions. Among the 11 genotypes characterized; CISH-A-33 and CISH-A-31 exhibited superior fruit yield per plant (53.99 ± 1.97 and 44.17 ± 0.91 kg/tree, respectively), indicating better adaptability to alkaline conditions. Additionally, CISH-A-33 genotypes showed higher content of biochemical traits such as total soluble solids (TSS) (9.70 ± 0.10°B), acidity (3.25 ± 0.10%), ascorbic acid (504.65 ± 1.12 mg/100 g), total sugar (8.21 ± 0.05%), reducing sugar (3.85 ± 0.01%), phenol (1.41 ± 0.01 g/100g GAE) and ferric reducing antioxidant power (FRAP) (233.74 ± 1.59 mg/g) followed by CISH-A-31. A correlation study between morphological and biochemical traits indicated that fruit weight was negatively correlated with TSS, ascorbic acid, total sugar and FRAP, but strongly positively correlated with yield, fruit diameter, acidity, reducing sugar and polyphenol content. TSS, total sugar and FRAP had a negative correlation with yield, whereas ascorbic acid, reducing sugar and polyphenols had a favourable correlation. Two principal components (PCs) with differing descriptive capacities were identified by principal component analysis; PC-1 explained 65.89% of the overall variability, while PC-2 explained 29.67%. These promising genotypes (CISH-A-33 and CISH-A-31) hold potential for crop genetic enhancement, benefiting alkaline soils not only in sub-tropical regions of India but also in other similar environments. This research identifies promising aonla accessions adaptable to alkaline soils, offering prospects for breeding climate-resilient varieties with enhanced productivity and fruit quality.