Introduction: The administration of antioxidant combination has been shown to be more effective than that of individual antioxidant in male infertility. Objective: to determine the effect of different combination of antioxidants (vitamin/vit C, vit E, glutathione and zinc) on the number of Sertoli and Leydig cells, sperm quality and texpression of caspase-3 in rats exposed to cigarette smoke. Methods: This was an experimental study using a post test only control group design. Thirty six rats were randomly divided into 6 groups to receive one of the following combinations of antioxidants: vit C and E, glutathione, zinc (T4-G); vit C and E, glutathione (T3-G); vit C and vit E (T2-G); Glutathione, zinc (T1-G); control positive (CP-G) cigarette smoke exposed only, normal (CN-G). The dose of vit C, vit E, glutathion and zinc was 9 mg/day, 1.8 IU/day, 1.8 mg/day, 0.2 mg/day respectively. All groups were exposed to cigarette smoke (3 cigarettes/day for 21 days), except for the CN-G group. On day 22, sperm samples were taken. Testicular tissue was taken for measurements of sperm quality, sertoli and leydig cell.numbers. Results: There was a significant difference in mean number of Leydig cells, sperm motility, sperm count and caspase-3 between groups (p<0.05). Post hoc LSD showed that the administration of combination of four antioxidant agent increasing the number of Sertoli cells and sperm morphology. The results also showed that group treated with 4 antioxidants combination had the highest number of Leydig cells, sperm motility, sperm counts and the lowest expression of caspase-3. Conclusion:The combination of vitC, vit E, glutathione and zinc affects the number of Sertoli and Leydig cells, sperm quality, expression of caspase-3 in rats exposed to cigarette smoke .

Introduction: Excessive fat accumulation in the obese child causes complex problems in the respiratory system. Respiratory system function can be improved by exercise. One exercise model that is suitable for children is Senam Sehat Anak Indonesia (SSAI). The quality of the respiratory function system is measured using Peak Expiratory Flow Rate (PEFR).Objective: to validate the PEFR’s differences in obese children before and after performing SSAI.Methods: This research using one group pre-posttest experimental design with total sample of 20 obese children using purposive sampling method. The research instrument uses weight scale, height scale, and peak flow meter. SSAI was given to all subjects twice a week for six weeks. Paired T Test statistical analysis was used to examine the PEFR’s differences before and after performing SSAI, considered significant if p&lt;0.05.Results: The PEFR’s mean of all subjects before performing SSAI was 258.25 ± 39.51 L∕minute, while PEFR’s mean after performing SSAI twice a week for 6 weeks was 282.50 ± 43.03 L∕minute, (p=0,000).Conclusion: The PEFR’s mean of obese children was increase significantly after performing SSAI twice a week for 6 weeks

Background:�Oxidative stress�and�inflammation�play�an important role in�pathogenesis of�brain aging�and neurodegenerative diseases such as�Alzheimer. Green tea�has been shown to have antioxidant, anti-inflammatory, anticancer, and neuroprotective�activity. Objectives: to determine the effect of green tea extract on�spatial memory function and superoxide dismutase�enzyme activity in mice with D-galactose induced dementiaMethods:�An experimental study using "post test only control group design".�Twenty male�BALB/c Mice aged 6-8�weeks were divided into�4�groups.�Negative control group�(NG)�was induced by subcutaneous injection of�D-galactose�(150�mg/kg BW)�once�daily for�6�weeks.�GT-90, GT-270,�GT-540�were induced by�D-galactose�and orally administered with 90, 270, and 540 mg/kg BW of�green tea extract�once daily for 6 weeks.�The spatial memory functions were assessed using Morris water maze and�SOD enzyme activities�were evaluated using ELISA.�One-way Anova and Kruskal-Wallis were used for statistical analysis.�Results: mean�percentage of latency time in the GT-90�(35.29�(SD=�2.69)%),�GT-270�(35.28 (SD= 2.62)%), and�GT-540�(35.62�(SD=5.05)%)�were�significantly�higher compared to that of NG�(20.38�(SD =�3.21)%), p�&lt;0.05). SOD enzyme activity in the�GT-270�(0.78 (SD = 0.07) U/ml)�was�significantly�higher�compared to that of NG�(0.51 (SD = 0.01) U�ml), p= 0.004).Conclusion:�Green tea extract�may�improve�spatial memory�function�and�the activity of�superoxide dismutase�enzyme in mice�with D-galactose induced�dementia.

Introduction: Stroke ranks as the first cause of disabilities and the third cause of death in the world. Many factors associated with stroke. Objective: this study aimed at evaluating the factors associated with stroke. Study Design: This study was an analytical observational study using cross sectional design. Samples were obtained from medical records of 263 patients who were admitted in neurology’s ward of Sultan agung Islamic hospital during 1 January 2012-31 August 2013. Samples categorized in 2 groups: 97 hemorrhagic’s stroke patients and 166 non hemorrhagic’s stroke patients with simple random sampling technique. This study were conducted by analyzing 5 variables such as gender, age, hypertension, diabetes mellitus and total cholesterol levels. Results: Logistic regression test using SPSS showed that hypertension was the most dominant factor associated with stroke (p=0.001; PR=1.707; CI=1.249–2.433). Conclusion: Hypertension was the most dominant risk factor for stroke.

Background: The tutorial plays a central role in problem-based learning (PBL). The effective implementation of small group tutorials is one of the key contributors to the students success in examinations. This study aimed to identify factors perceived as the predictors for effectiveness of PBL group tutorial.Design and method: The subjects of the study were all of the students (year of 2008) of the Medical College of Sultan Agung Islamic University taking the Tropical Disease Module. The adapted version of the scales adopted in this study included the scale developed by Van den Bossche et al for assessing team (group) effectiveness, trust dimension and learning behavior, a scale developed by Dolmans &amp; Ginns (2005) for assessing tutor’s performance, a 5 points likert scale was used to asses the student opinions. Quantitative approach with correlation and multiple regression analysis was applied.Result: Of 238 students included in the study 84 (37.3%) were male and 139 (62.3%) were female. There was a significantly positive correlation between the trust dimension related to the interpersonal relationship among the members of tutorial group with the effectiveness of tutorial group ( r = 0.631, p&lt; 0.01). There was a medium correlation among cohesion, group potency and psychological (r= 0,700, p &lt; 0.05 there was correlation among the variables of (r = 0,557, p &lt; 0,001), group potency (r = 0,517, p &lt; 0,001), and psychological assurance (r = 0,437, p &lt; 0.001). These three variables contributed to the group effectiveness as much as 48.4% with a F value of 87.857. The component of independence was not found to be the predictive factors for group effectiveness. The correlations among the variables of cohesion, group potency and psychological assurance resulted in contingency coeficien of 0.700 with p&lt;0.05.Conclusion: The higher trust related to the interpersonal relationship among the member of groups, the more effective the group will be. Cohesion, group potency and psychological assurance were found to be the predictive factors for group effectiveness (Sains Medika, 3(1):16-23).